CA2869891A1 - Micro-organismes genetiquement modifies produisant du pha - Google Patents

Micro-organismes genetiquement modifies produisant du pha Download PDF

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Publication number
CA2869891A1
CA2869891A1 CA2869891A CA2869891A CA2869891A1 CA 2869891 A1 CA2869891 A1 CA 2869891A1 CA 2869891 A CA2869891 A CA 2869891A CA 2869891 A CA2869891 A CA 2869891A CA 2869891 A1 CA2869891 A1 CA 2869891A1
Authority
CA
Canada
Prior art keywords
pha
microorganism
ppu
genetically engineered
synthase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2869891A
Other languages
English (en)
Inventor
Sagrario ARIAS
Monica BASSAS
Gabriella Molinari
Kenneth Nigel Timmes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Original Assignee
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helmholtz Zentrum fuer Infektionsforschung HZI GmbH filed Critical Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Publication of CA2869891A1 publication Critical patent/CA2869891A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/874—Pseudomonas

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2869891A 2012-04-11 2013-04-11 Micro-organismes genetiquement modifies produisant du pha Abandoned CA2869891A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12163787.0 2012-04-11
EP12163787 2012-04-11
PCT/EP2013/057630 WO2013153180A1 (fr) 2012-04-11 2013-04-11 Micro-organismes génétiquement modifiés produisant du pha

Publications (1)

Publication Number Publication Date
CA2869891A1 true CA2869891A1 (fr) 2013-10-17

Family

ID=48128294

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2869891A Abandoned CA2869891A1 (fr) 2012-04-11 2013-04-11 Micro-organismes genetiquement modifies produisant du pha

Country Status (11)

Country Link
US (1) US20150203878A1 (fr)
EP (1) EP2836601A1 (fr)
JP (1) JP2015512648A (fr)
KR (1) KR20150004830A (fr)
CN (1) CN104520433A (fr)
CA (1) CA2869891A1 (fr)
HK (1) HK1206787A1 (fr)
IN (1) IN2014MN02184A (fr)
RU (1) RU2014144504A (fr)
SG (1) SG11201405636QA (fr)
WO (1) WO2013153180A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5288007B2 (ja) * 2010-07-14 2013-09-11 トヨタ自動車株式会社 変異型ポリヒドロキシアルカン酸シンターゼ遺伝子及びこれを用いた脂肪族ポリエステルの製造方法
JP6313708B2 (ja) 2012-10-22 2018-04-18 株式会社カネカ 高分子量ｐｈａ生産微生物とそれを用いた高分子量ｐｈａの製造方法
CN104328062A (zh) * 2014-06-13 2015-02-04 东北师范大学 一株假单胞菌诱变菌株及其在生产(r)-3-羟基丁酸中的应用
EP3551586A4 (fr) * 2016-12-09 2020-07-29 Knipbio, Inc. Production microbienne de protéine et de phb par des bactéries utilisant de l'alcool
CN110004182B (zh) * 2019-04-02 2021-03-05 清华大学 一种微生物胞内大颗粒内含物的制备方法及其应用
CN111909952B (zh) * 2020-08-04 2024-04-26 北京大学深圳研究院 构建食烷菌敲除酰基辅酶a硫脂酶基因的方法和应用
CN111982875A (zh) * 2020-08-20 2020-11-24 北京大学深圳研究院 一种基于三维荧光光谱分析的产聚羟基脂肪酸酯菌的筛选方法
CN115261346B (zh) * 2022-04-06 2023-04-07 深圳蓝晶生物科技有限公司 表达乙酰乙酰辅酶a还原酶变体的工程化微生物及提高pha产量的方法
CN116445440B (zh) * 2022-10-26 2024-01-02 华南理工大学 黏性聚羟基脂肪酸酯及其制备和应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4417169A1 (de) * 1994-05-17 1995-11-23 Vogelbusch Gmbh Rekombinante Herstellung von Polyhydroxyalkanoaten in glucosefreiem Medium
DE69838768T2 (de) * 1997-09-19 2008-10-30 Metabolix, Inc., Cambridge Biologische Systeme zur Herstellung von Polyhydroxyalkanoat-Polymeren die 4-Hy droxysäure enthalten

Also Published As

Publication number Publication date
US20150203878A1 (en) 2015-07-23
IN2014MN02184A (fr) 2015-08-28
CN104520433A (zh) 2015-04-15
KR20150004830A (ko) 2015-01-13
WO2013153180A1 (fr) 2013-10-17
EP2836601A1 (fr) 2015-02-18
RU2014144504A (ru) 2016-06-10
SG11201405636QA (en) 2014-11-27
HK1206787A1 (en) 2016-01-15
JP2015512648A (ja) 2015-04-30

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Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20170411