CA2864276C - Improved methods of nucleic acid sequencing - Google Patents

Improved methods of nucleic acid sequencing Download PDF

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Publication number
CA2864276C
CA2864276C CA2864276A CA2864276A CA2864276C CA 2864276 C CA2864276 C CA 2864276C CA 2864276 A CA2864276 A CA 2864276A CA 2864276 A CA2864276 A CA 2864276A CA 2864276 C CA2864276 C CA 2864276C
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dna
nucleic acid
duplexes
strand
rna
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French (fr)
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CA2864276A1 (en
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Niall Anthony Gormley
Louise Fraser
Paula KOKKO-GONZALES
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Illumina Cambridge Ltd
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Illumina Cambridge Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
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  • General Physics & Mathematics (AREA)
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CA2864276A 2012-03-06 2013-03-06 Improved methods of nucleic acid sequencing Active CA2864276C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261607418P 2012-03-06 2012-03-06
US61/607,418 2012-03-06
PCT/EP2013/054517 WO2013131962A1 (en) 2012-03-06 2013-03-06 Improved methods of nucleic acid sequencing

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CA2864276A1 CA2864276A1 (en) 2013-09-12
CA2864276C true CA2864276C (en) 2020-05-05

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CA2864276A Active CA2864276C (en) 2012-03-06 2013-03-06 Improved methods of nucleic acid sequencing

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US (4) US9574226B2 (en:Method)
EP (2) EP2823058B1 (en:Method)
JP (2) JP6068517B2 (en:Method)
CN (1) CN104160040B (en:Method)
AU (1) AU2013229533B2 (en:Method)
CA (1) CA2864276C (en:Method)
DK (2) DK2823058T3 (en:Method)
ES (1) ES2565809T3 (en:Method)
NO (1) NO2694769T3 (en:Method)
TR (1) TR201807138T4 (en:Method)
WO (1) WO2013131962A1 (en:Method)

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