CA2840568C - Compositions, uses, and preparation of platelet lysates - Google Patents

Compositions, uses, and preparation of platelet lysates Download PDF

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Publication number
CA2840568C
CA2840568C CA2840568A CA2840568A CA2840568C CA 2840568 C CA2840568 C CA 2840568C CA 2840568 A CA2840568 A CA 2840568A CA 2840568 A CA2840568 A CA 2840568A CA 2840568 C CA2840568 C CA 2840568C
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Prior art keywords
composition
cells
stem cells
fibrinogen
platelet lysate
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CA2840568A
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English (en)
French (fr)
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CA2840568A1 (en
Inventor
Ian B. Copland
Jacques Galipeau
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Emory University
Childrens Healthcare of Atlanta Inc
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Emory University
Childrens Healthcare of Atlanta Inc
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14Blood; Artificial blood
    • A61K35/19Platelets; Megacaryocytes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/70Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/726Glycosaminoglycans, i.e. mucopolysaccharides
    • A61K31/727Heparin; Heparan
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18Growth factors; Growth regulators
    • A61K38/1858Platelet-derived growth factor [PDGF]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/40Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42Use of materials characterised by their function or physical properties
    • A61L15/44Medicaments
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L17/00Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters
    • A61L17/005Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters containing a biologically active substance, e.g. a medicament or a biocide
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/0005Ingredients of undetermined constitution or reaction products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/001Use of materials characterised by their function or physical properties
    • A61L24/0015Medicaments; Biocides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • A61P17/02Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00Drugs for disorders of the senses
    • A61P27/02Ophthalmic agents
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
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    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
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    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0663Bone marrow mesenchymal stem cells (BM-MSC)
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    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0664Dental pulp stem cells, Dental follicle stem cells
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    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0665Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0666Mesenchymal stem cells from hair follicles
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0667Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0668Mesenchymal stem cells from other natural sources
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404Biocides, antimicrobial agents, antiseptic agents
    • A61L2300/406Antibiotics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/42Anti-thrombotic agents, anticoagulants, anti-platelet agents
    • CCHEMISTRY; METALLURGY
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    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/11Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/115Platelets, megakaryocytes

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  • Developmental Biology & Embryology (AREA)
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  • Public Health (AREA)
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  • Cell Biology (AREA)
  • Epidemiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Materials Engineering (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Surgery (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Dermatology (AREA)
  • Virology (AREA)
  • Vascular Medicine (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Ophthalmology & Optometry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CA2840568A 2011-06-27 2012-06-26 Compositions, uses, and preparation of platelet lysates Active CA2840568C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161501411P 2011-06-27 2011-06-27
US61/501,411 2011-06-27
US201161547897P 2011-10-17 2011-10-17
US61/547,897 2011-10-17
PCT/US2012/044211 WO2013003356A1 (en) 2011-06-27 2012-06-26 Compositions, uses, and preparation of platelet lysates

Publications (2)

Publication Number Publication Date
CA2840568A1 CA2840568A1 (en) 2013-01-03
CA2840568C true CA2840568C (en) 2021-05-04

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Application Number Title Priority Date Filing Date
CA2840568A Active CA2840568C (en) 2011-06-27 2012-06-26 Compositions, uses, and preparation of platelet lysates

Country Status (8)

Country Link
US (2) US9688959B2 (enExample)
EP (1) EP2723354B3 (enExample)
JP (1) JP6179955B2 (enExample)
CN (2) CN108671224B (enExample)
AU (1) AU2012275562B2 (enExample)
CA (1) CA2840568C (enExample)
ES (1) ES2811085T7 (enExample)
WO (1) WO2013003356A1 (enExample)

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AU2014312421A1 (en) * 2013-08-27 2016-04-14 Cook General Biotechnology Llc Bioactive compositions derivable from platelet concentrates, and methods for preparing and using same
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WO2015148723A1 (en) * 2014-03-25 2015-10-01 Cook Biotech Incorporated Extracellular matrix grafts loaded with exogenous factors
CA2974225C (en) * 2015-01-21 2023-08-08 Edvin TURKOF Platelet concentrate for increase of cell regeneration and cell growth
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WO2016196515A1 (en) * 2015-05-29 2016-12-08 Amit Patel Particulate lyophilized platelet lysate compositions
CN107304412A (zh) * 2016-04-22 2017-10-31 南京医科大学第附属医院 视网膜色素上皮细胞的培养基及其应用
EP3254684B1 (en) 2016-06-08 2019-10-23 Lysatpharma GmbH Human platelet lysate or fraction enriched in human platelet-derived extracellular vesicles, for use in medicine
WO2018005751A1 (en) * 2016-06-29 2018-01-04 Arteriocyte, Inc. Dressings comprising platelet lysate
EP3558411B1 (en) * 2016-12-22 2024-04-17 Regenexx, LLC Device and methods for platelet lysis or activation
IT201700026465A1 (it) * 2017-03-09 2018-09-09 Biorigen S R L Supplementi per colture cellulari
CA3067294A1 (en) 2017-06-16 2018-12-20 Centre Hospitalier Regional Et Universitaire De Lille (Chru) Process for preparing a pooled human platelet lysate, pooled human platelet lysate and its use for treating neurological disorders
JP7286651B2 (ja) * 2017-12-22 2023-06-05 キエージ・フアルマチエウテイチ・エツセ・ピ・ア 間葉系間質細胞及び臍帯から間葉系間質細胞を得るための方法
FR3077824B1 (fr) 2018-02-15 2022-01-14 Maco Pharma Sa Procede de preparation d'un lysat plaquettaire irradie
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CN108823156A (zh) * 2018-07-04 2018-11-16 陕西神州生物技术有限公司 用于修复的临床级人脐带间充质干细胞复合因子及冻干粉的制备方法
JP2021534231A (ja) * 2018-08-07 2021-12-09 アイフィックス メディカル プロプライアタリー リミティド 血小板溶解物組成物およびその使用
PL3880217T3 (pl) * 2018-11-14 2024-05-20 Onderzoeks en Ontwikkelingsfonds Rode Kruis-Vlaanderen Sposoby otrzymywania materiału uwalnianego z płytek krwi
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CA3152224A1 (en) * 2019-08-22 2021-02-25 Abdalla S. AWIDI Method of preparing a de-fibrinated platelet lysate, and uses of said method
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WO2023190736A1 (ja) * 2022-03-29 2023-10-05 学校法人日本大学 ヒト脱分化脂肪細胞の製造方法及びヒト成熟脂肪細胞からヒト脱分化脂肪細胞を製造するための培地
EP4276173A1 (en) * 2022-05-10 2023-11-15 Stemmatters, Biotecnologia e Medicina Regenerativa, S.A. Stable human platelet lysate composition, methods and uses thereof
CN120225662A (zh) * 2022-12-06 2025-06-27 苏州血霁生物科技有限公司 一种分化血小板的方法、培养基及应用
JP7731557B2 (ja) 2022-12-30 2025-09-01 スピリット サイエンティフィック カンパニー リミテッド 血小板含有組成物及びその製造方法

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