CA2822254A1 - Methods and compositions for detecting mutation in the human epidermal growth factor receptor gene - Google Patents

Methods and compositions for detecting mutation in the human epidermal growth factor receptor gene Download PDF

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Publication number
CA2822254A1
CA2822254A1 CA2822254A CA2822254A CA2822254A1 CA 2822254 A1 CA2822254 A1 CA 2822254A1 CA 2822254 A CA2822254 A CA 2822254A CA 2822254 A CA2822254 A CA 2822254A CA 2822254 A1 CA2822254 A1 CA 2822254A1
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CA
Canada
Prior art keywords
seq
oligonucleotide
nos
oligonucleotides
primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2822254A
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English (en)
French (fr)
Inventor
Keith Bauer
Nancy Schoenbrunner
Alison Tsan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2822254A1 publication Critical patent/CA2822254A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2822254A 2010-12-22 2011-12-17 Methods and compositions for detecting mutation in the human epidermal growth factor receptor gene Abandoned CA2822254A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201061426436P 2010-12-22 2010-12-22
US61/426,436 2010-12-22
PCT/EP2011/006399 WO2012084173A2 (en) 2010-12-22 2011-12-17 Methods and compositions for detecting mutation in the human epidermal growth factor receptor gene

Publications (1)

Publication Number Publication Date
CA2822254A1 true CA2822254A1 (en) 2012-06-28

Family

ID=45478263

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2822254A Abandoned CA2822254A1 (en) 2010-12-22 2011-12-17 Methods and compositions for detecting mutation in the human epidermal growth factor receptor gene

Country Status (8)

Country Link
US (1) US20120164641A1 (https=)
EP (1) EP2655659A2 (https=)
JP (1) JP2014500028A (https=)
KR (1) KR20130094342A (https=)
CN (1) CN103282515A (https=)
AU (1) AU2011348483A1 (https=)
CA (1) CA2822254A1 (https=)
WO (1) WO2012084173A2 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
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JP6050820B2 (ja) 2011-09-23 2016-12-21 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改良された対立遺伝子特異的ｐｃｒのためのｇ−クランプの使用
US9382581B2 (en) 2012-12-13 2016-07-05 Roche Molecular Systems, Inc. Primers with modified phosphate and base in allele-specific PCR
US9279146B2 (en) 2012-12-21 2016-03-08 Roche Molecular Systems, Inc. Compounds and methods for the enrichment of mutated nucleic acid from a mixture
US9873908B2 (en) 2013-11-27 2018-01-23 Roche Molecular Systems, Inc. Methods for the enrichment of mutated nucleic acid from a mixture
CN104087674B (zh) * 2014-07-15 2016-02-10 江苏同科医药科技有限公司 一种人表皮生长因子受体突变基因检测试剂盒
WO2016055380A1 (en) * 2014-10-09 2016-04-14 Roche Diagnostics Gmbh Mutations in the epidermal growth factor receptor kinase domain
CZ308881B6 (cs) * 2014-12-09 2021-08-04 Univerzita Palackého v Olomouci 6-aryl-9-glykosylpuriny a jejich použití
CN105177156B (zh) * 2015-10-12 2018-04-10 苏州华益美生物科技有限公司 人egfr基因突变检测试剂盒及其应用
CN108676848B (zh) * 2018-05-31 2022-04-22 上海科医联创医学检验所有限公司 用于检测融合基因的混合基因、标准质粒、试剂盒及其制备方法
CN111607593A (zh) * 2019-02-26 2020-09-01 成都华青精准医疗科技有限公司 一种用于检测egfr基因突变的核苷酸序列组及其应用
CN113355423B (zh) * 2021-07-07 2022-06-07 安徽科技学院 Egfr基因l858r突变检测的引物探针、试剂盒及其应用
CN117106865B (zh) * 2023-08-23 2024-05-10 北京医院 基于双扩增放大和双lna探针特异识别dna突变的高灵敏检测方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981725A (en) * 1989-09-08 1999-11-09 The Johns Hopkins Univiersity Structural alterations of the EGF receptor gene in human tumors
US5981176A (en) 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
ATE280177T1 (de) * 1997-03-20 2004-11-15 Hoffmann La Roche Modifizierte primer
AU2520799A (en) * 1998-02-05 1999-08-23 Bavarian Nordic Research Institute A/S Quantification by inhibition of amplification
US6235480B1 (en) * 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
AU2002210791A1 (en) * 2000-07-28 2002-02-13 Compugen Inc. Oligonucleotide library for detecting rna transcripts and splice variants that populate a transcriptome
CN101501211A (zh) 2004-03-31 2009-08-05 综合医院公司 测定癌症对表皮生长因子受体靶向性治疗反应性的方法
EP1766068A4 (en) 2004-06-04 2010-03-03 Genentech Inc EGFR Mutations
MX2007009963A (es) * 2005-02-24 2007-09-26 Amgen Inc Mutaciones del receptor del factor de crecimiento epidermico.
GB2424886A (en) * 2005-04-04 2006-10-11 Dxs Ltd Polynucleotide primers against epidermal growth factor receptor and method of detecting gene mutations
CN1710102A (zh) * 2005-06-20 2005-12-21 上海市肺科医院 一种肿瘤相关基因突变的pcr检测方法及试剂系统
US20070072211A1 (en) * 2005-06-30 2007-03-29 Roche Molecular Systems, Inc. Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids
CN101041850A (zh) * 2006-03-20 2007-09-26 吕成伟 一种人类表皮生长因子受体（egfr）基因外显子20的t790m突变快速检测方法和试剂盒
US8598333B2 (en) * 2006-05-26 2013-12-03 Alnylam Pharmaceuticals, Inc. SiRNA silencing of genes expressed in cancer
ES2527653T3 (es) 2008-10-20 2015-01-28 F. Hoffmann-La Roche Ag Amplificación específica de alelo usando un cebador con un nucleótido modificado
US20100143901A1 (en) 2008-12-09 2010-06-10 Roche Molecular Systems, Inc. Nuclease-Free Real-Time Detection of Nucleic Acids

Also Published As

Publication number Publication date
JP2014500028A (ja) 2014-01-09
US20120164641A1 (en) 2012-06-28
EP2655659A2 (en) 2013-10-30
AU2011348483A1 (en) 2013-06-13
CN103282515A (zh) 2013-09-04
WO2012084173A2 (en) 2012-06-28
KR20130094342A (ko) 2013-08-23
WO2012084173A3 (en) 2012-10-26

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20130619

FZDE Discontinued

Effective date: 20160310