CA2810757C - Mutant channelrhodopsin 2 - Google Patents

Mutant channelrhodopsin 2 Download PDF

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Publication number
CA2810757C
CA2810757C CA2810757A CA2810757A CA2810757C CA 2810757 C CA2810757 C CA 2810757C CA 2810757 A CA2810757 A CA 2810757A CA 2810757 A CA2810757 A CA 2810757A CA 2810757 C CA2810757 C CA 2810757C
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Prior art keywords
light
cell
ion channel
cys
inducible ion
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CA2810757A
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English (en)
French (fr)
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CA2810757A1 (en
Inventor
Ernst Bamberg
Christian Bamann
Sonja Kleinlogel
Phillip Wood
Robert E. Dempski
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Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Ophthalmology & Optometry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2810757A 2010-09-08 2011-09-08 Mutant channelrhodopsin 2 Active CA2810757C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US38079310P 2010-09-08 2010-09-08
EP10175832 2010-09-08
US61/380,793 2010-09-08
EP10175832.4 2010-09-08
PCT/EP2011/065510 WO2012032103A1 (en) 2010-09-08 2011-09-08 Mutant channelrhodopsin 2

Publications (2)

Publication Number Publication Date
CA2810757A1 CA2810757A1 (en) 2012-03-15
CA2810757C true CA2810757C (en) 2020-06-09

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2810757A Active CA2810757C (en) 2010-09-08 2011-09-08 Mutant channelrhodopsin 2

Country Status (20)

Country Link
US (1) US8748578B2 (cg-RX-API-DMAC10.html)
EP (1) EP2614079B1 (cg-RX-API-DMAC10.html)
JP (1) JP5933556B2 (cg-RX-API-DMAC10.html)
KR (1) KR101945962B1 (cg-RX-API-DMAC10.html)
CN (1) CN103261219B (cg-RX-API-DMAC10.html)
AU (1) AU2011298745B2 (cg-RX-API-DMAC10.html)
BR (1) BR112013005634A2 (cg-RX-API-DMAC10.html)
CA (1) CA2810757C (cg-RX-API-DMAC10.html)
DK (1) DK2614079T3 (cg-RX-API-DMAC10.html)
ES (1) ES2545992T3 (cg-RX-API-DMAC10.html)
HR (1) HRP20150846T1 (cg-RX-API-DMAC10.html)
HU (1) HUE025952T2 (cg-RX-API-DMAC10.html)
IL (1) IL225060A (cg-RX-API-DMAC10.html)
MX (1) MX347453B (cg-RX-API-DMAC10.html)
PL (1) PL2614079T3 (cg-RX-API-DMAC10.html)
PT (1) PT2614079E (cg-RX-API-DMAC10.html)
RU (1) RU2595384C2 (cg-RX-API-DMAC10.html)
SG (1) SG188409A1 (cg-RX-API-DMAC10.html)
SI (1) SI2614079T1 (cg-RX-API-DMAC10.html)
WO (1) WO2012032103A1 (cg-RX-API-DMAC10.html)

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US9238150B2 (en) 2005-07-22 2016-01-19 The Board Of Trustees Of The Leland Stanford Junior University Optical tissue interface method and apparatus for stimulating cells
US20090093403A1 (en) 2007-03-01 2009-04-09 Feng Zhang Systems, methods and compositions for optical stimulation of target cells
US8926959B2 (en) 2005-07-22 2015-01-06 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US10052497B2 (en) 2005-07-22 2018-08-21 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
JP2009502140A (ja) 2005-07-22 2009-01-29 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 光活性化陽イオンチャネルおよびその使用
US9274099B2 (en) 2005-07-22 2016-03-01 The Board Of Trustees Of The Leland Stanford Junior University Screening test drugs to identify their effects on cell membrane voltage-gated ion channel
US8470790B2 (en) 2006-05-04 2013-06-25 Wayne State University Restoration of visual responses by in vivo delivery of rhodopsin nucleic acids
US8398692B2 (en) 2007-01-10 2013-03-19 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US8401609B2 (en) 2007-02-14 2013-03-19 The Board Of Trustees Of The Leland Stanford Junior University System, method and applications involving identification of biological circuits such as neurological characteristics
US10434327B2 (en) 2007-10-31 2019-10-08 The Board Of Trustees Of The Leland Stanford Junior University Implantable optical stimulators
US10035027B2 (en) 2007-10-31 2018-07-31 The Board Of Trustees Of The Leland Stanford Junior University Device and method for ultrasonic neuromodulation via stereotactic frame based technique
ES2701349T3 (es) 2008-04-23 2019-02-21 Univ Leland Stanford Junior Sistemas, métodos y composiciones para la estimulación óptica de células diana
EP2294208B1 (en) 2008-05-29 2013-05-08 The Board of Trustees of The Leland Stanford Junior University Cell line, system and method for optical control of secondary messengers
EP2303405A4 (en) 2008-06-17 2017-12-27 The Board of Trustees of the Leland Stanford Junior University Apparatus and methods for controlling cellular development
JP2011525130A (ja) 2008-06-17 2011-09-15 ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティ 光伝送素子を用いた標的細胞の光刺激のための方法、システム、および装置
WO2010006049A1 (en) 2008-07-08 2010-01-14 The Board Of Trustees Of The Leland Stanford Junior University Materials and approaches for optical stimulation of the peripheral nervous system
NZ602416A (en) 2008-11-14 2014-08-29 Univ Leland Stanford Junior Optically-based stimulation of target cells and modifications thereto
EP3399024A1 (en) 2010-03-17 2018-11-07 The Board of Trustees of The Leland Stanford Junior University Light-sensitive ion-passing molecules
WO2011140279A1 (en) 2010-05-04 2011-11-10 Wayne State University Aav-mediated subcellular targeting of heterologous rhodopsins in retinal ganglion cells
CN103384469B (zh) 2010-11-05 2016-06-15 斯坦福大学托管董事会 光控cns功能障碍
US10086012B2 (en) 2010-11-05 2018-10-02 The Board Of Trustees Of The Leland Stanford Junior University Control and characterization of memory function
ES2625179T3 (es) 2010-11-05 2017-07-18 The Board Of Trustees Of The Leland Stanford Junior University Control optogenético de comportamientos relacionados con la recompensa
CN106422081B (zh) 2010-11-05 2019-06-21 斯坦福大学托管董事会 用于光遗传学方法的光的上转换
US10568307B2 (en) 2010-11-05 2020-02-25 The Board Of Trustees Of The Leland Stanford Junior University Stabilized step function opsin proteins and methods of using the same
EP3486253A1 (en) 2010-11-05 2019-05-22 The Board of Trustees of The Leland Stanford Junior University Light-activated chimeric opsins and methods of using the same
US8696722B2 (en) 2010-11-22 2014-04-15 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic magnetic resonance imaging
CA2859364C (en) 2011-12-16 2019-05-07 The Board Of Trustees Of The Leland Stanford Junior University Opsin polypeptides and methods of use thereof
ES2628006T3 (es) 2012-02-21 2017-08-01 Circuit Therapeutics, Inc. Composiciones para el tratamiento de trastornos neurogénicos del suelo pélvico
EA201491643A1 (ru) 2012-03-05 2015-02-27 Уэйн Стейт Юниверсити Идентификация мутаций канал-опсина-2 (chop2) и способы применения
ES2742492T3 (es) 2013-03-15 2020-02-14 Univ Leland Stanford Junior Control optogenético del estado conductual
US9636380B2 (en) 2013-03-15 2017-05-02 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic control of inputs to the ventral tegmental area
US10220092B2 (en) 2013-04-29 2019-03-05 The Board Of Trustees Of The Leland Stanford Junior University Devices, systems and methods for optogenetic modulation of action potentials in target cells
JP6621747B2 (ja) 2013-08-14 2019-12-18 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 疼痛を制御するための組成物及び方法
JP6118239B2 (ja) * 2013-11-29 2017-04-19 雅敏 堀 害虫の防除方法及び防除装置
WO2015167639A1 (en) * 2014-02-07 2015-11-05 Massachusetts Institute Of Technology Blue light-activated ion channel molecules and uses thereof
US10047130B2 (en) 2014-03-18 2018-08-14 Washington University Methods and compositions for red-shifted chromophore substitution for optogenetic applications
WO2015161308A1 (en) * 2014-04-18 2015-10-22 Massachusetts Institute Of Technology Mutant channelrhodopsins with altered ion selectivity
US10568516B2 (en) 2015-06-22 2020-02-25 The Board Of Trustees Of The Leland Stanford Junior University Methods and devices for imaging and/or optogenetic control of light-responsive neurons
US11384132B2 (en) 2016-06-03 2022-07-12 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Mutant light-inducible ion channel of chrimson
EP3464341A1 (en) * 2016-06-03 2019-04-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Mutant light-inducible ion channel of channelrhodopsin
WO2018044912A1 (en) 2016-08-29 2018-03-08 Wayne State University Identification of mutations in channelopsin variants having improved light sensitivity and methods of use thereof
EP3535282A4 (en) * 2016-11-06 2020-04-08 Nanoscope Technologies LLC OPTOGENETIC MODULATION BY OPSINS WITH MULTIPLE CHARACTERISTICS FOR RESTORATION OF VISION AND OTHER ASSOCIATED APPLICATIONS
US11294165B2 (en) 2017-03-30 2022-04-05 The Board Of Trustees Of The Leland Stanford Junior University Modular, electro-optical device for increasing the imaging field of view using time-sequential capture
US20200115419A1 (en) * 2017-04-12 2020-04-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. New optogenetic tool
CN110483645B (zh) * 2018-05-14 2023-05-12 郑州大学第一附属医院 线粒体定位质子泵型视紫红质、其突变蛋白及其应用
JP7242851B2 (ja) * 2018-07-26 2023-03-20 カウンシル・オブ・サイエンティフィック・アンド・インダストリアル・リサーチ・アン・インディアン・レジスタード・ボディ・インコーポレイテッド・アンダー・ザ・レジストレーション・オブ・ソサエティーズ・アクト・(アクト・21・オブ・1860) 子宮頸がんのグレード検出のためのスクリーニングキットおよびその調製方法
CN112689675B (zh) * 2018-09-14 2023-09-19 国立大学法人名古屋工业大学 光响应性蛋白及其应用
US20230104240A1 (en) * 2020-03-13 2023-04-06 Nagoya Institute Of Technology Photoresponsive protein for color recognition and use thereof
WO2022176754A1 (ja) * 2021-02-18 2022-08-25 国立大学法人名古屋工業大学 青色光もしくは藍色光応答性タンパク質及びその利用
EP4223768B1 (en) * 2022-02-04 2026-05-06 Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin Novel mutant bacteriorhodopsin-like-channelrhodopsin ion channel
WO2024092248A1 (en) * 2022-10-28 2024-05-02 The Board Of Trustees Of The Leland Stanford Junior University Enhanced light gated potassium selective channelrhodopsin

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DE10216005A1 (de) * 2002-04-11 2003-10-30 Max Planck Gesellschaft Verwendung von biologischen Photorezeptoren als direkt lichtgesteuerte Ionenkanäle
US8470790B2 (en) * 2006-05-04 2013-06-25 Wayne State University Restoration of visual responses by in vivo delivery of rhodopsin nucleic acids
DE102008020152B4 (de) * 2008-04-22 2012-04-05 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Verwendung von biologischen Photorezeptoren als direkt lichtgesteuerte Ionenkanäle
NZ602416A (en) * 2008-11-14 2014-08-29 Univ Leland Stanford Junior Optically-based stimulation of target cells and modifications thereto

Also Published As

Publication number Publication date
PT2614079E (pt) 2015-10-06
MX2013002591A (es) 2013-07-22
KR20130108335A (ko) 2013-10-02
HRP20150846T1 (hr) 2015-09-11
CN103261219B (zh) 2017-05-17
US20130281379A1 (en) 2013-10-24
MX347453B (es) 2017-04-27
SI2614079T1 (sl) 2015-10-30
PL2614079T3 (pl) 2016-01-29
JP5933556B2 (ja) 2016-06-15
KR101945962B1 (ko) 2019-02-08
WO2012032103A1 (en) 2012-03-15
CN103261219A (zh) 2013-08-21
AU2011298745B2 (en) 2015-05-07
DK2614079T3 (en) 2015-08-10
JP2013544494A (ja) 2013-12-19
EP2614079B1 (en) 2015-07-15
US8748578B2 (en) 2014-06-10
CA2810757A1 (en) 2012-03-15
EP2614079A1 (en) 2013-07-17
IL225060A (en) 2016-10-31
RU2595384C2 (ru) 2016-08-27
RU2013115454A (ru) 2014-10-20
AU2011298745A1 (en) 2013-03-21
BR112013005634A2 (pt) 2017-06-27
ES2545992T3 (es) 2015-09-17
SG188409A1 (en) 2013-04-30
HUE025952T2 (en) 2016-04-28

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