CA2806858C - Reprogramming immortalized b cells - Google Patents
Reprogramming immortalized b cells Download PDFInfo
- Publication number
- CA2806858C CA2806858C CA2806858A CA2806858A CA2806858C CA 2806858 C CA2806858 C CA 2806858C CA 2806858 A CA2806858 A CA 2806858A CA 2806858 A CA2806858 A CA 2806858A CA 2806858 C CA2806858 C CA 2806858C
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- Prior art keywords
- cells
- pluripotent stem
- induced pluripotent
- reprogramming
- immortalized
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000008672 reprogramming Effects 0.000 title abstract 2
- 210000003719 b-lymphocyte Anatomy 0.000 abstract 2
- 239000013598 vector Substances 0.000 abstract 2
- 241000701044 Human gammaherpesvirus 4 Species 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 abstract 1
- 230000008707 rearrangement Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/06—Anti-neoplasic drugs, anti-retroviral drugs, e.g. azacytidine, cyclophosphamide
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/235—Leukemia inhibitory factor [LIF]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/602—Sox-2
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/603—Oct-3/4
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/604—Klf-4
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- C12N2501/60—Transcription factors
- C12N2501/605—Nanog
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/606—Transcription factors c-Myc
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/608—Lin28
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2510/00—Genetically modified cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2510/00—Genetically modified cells
- C12N2510/04—Immortalised cells
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- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16211—Lymphocryptovirus, e.g. human herpesvirus 4, Epstein-Barr Virus
- C12N2710/16241—Use of virus, viral particle or viral elements as a vector
- C12N2710/16243—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Developmental Biology & Embryology (AREA)
- Transplantation (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37061510P | 2010-08-04 | 2010-08-04 | |
| US61/370,615 | 2010-08-04 | ||
| US201161441885P | 2011-02-11 | 2011-02-11 | |
| US61/441,885 | 2011-02-11 | ||
| PCT/US2011/046452 WO2012018933A2 (en) | 2010-08-04 | 2011-08-03 | Reprogramming immortalized b cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2806858A1 CA2806858A1 (en) | 2012-02-09 |
| CA2806858C true CA2806858C (en) | 2021-06-15 |
Family
ID=45560057
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2806858A Active CA2806858C (en) | 2010-08-04 | 2011-08-03 | Reprogramming immortalized b cells |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US8765470B2 (enExample) |
| EP (1) | EP2601289B1 (enExample) |
| JP (2) | JP5936218B2 (enExample) |
| CA (1) | CA2806858C (enExample) |
| WO (1) | WO2012018933A2 (enExample) |
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087991B (zh) | 2009-06-05 | 2018-06-12 | 富士胶片细胞动力公司 | 重编程t细胞和造血细胞的方法 |
| WO2011016588A1 (en) | 2009-08-07 | 2011-02-10 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
| CN106893692B (zh) * | 2010-12-22 | 2021-11-26 | 菲特治疗公司 | 用于单细胞分选与增强ipsc重新编程的细胞培养平台 |
| JP2014520551A (ja) * | 2011-07-11 | 2014-08-25 | セルラー ダイナミクス インターナショナル, インコーポレイテッド | 細胞のリプログラミング方法およびゲノムの改変方法 |
| CN102604894B (zh) * | 2012-02-29 | 2014-07-30 | 中国科学院广州生物医药与健康研究院 | 用于制备神经干细胞的培养基及其用途 |
| EP2853592B1 (en) * | 2012-05-23 | 2019-02-20 | Kyoto University | Highly efficient method for establishing artificial pluripotent stem cell |
| EP2971039B1 (en) | 2013-03-14 | 2020-01-01 | Immusoft Corporation | Methods for in vitro memory b cell differentiation and transduction with vsv-g pseudotyped viral vectors |
| US20160115455A1 (en) * | 2013-05-17 | 2016-04-28 | The Broad Institute, Inc. | Reprogrammed cells and methods of production and use thereof |
| WO2015006725A2 (en) | 2013-07-12 | 2015-01-15 | Cedars-Sinai Medical Center | Generation of induced pluripotent stem cells from normal human mammary epithelial cells |
| SG10201807292YA (en) | 2014-03-04 | 2018-09-27 | Fate Therapeutics Inc | Improved reprogramming methods and cell culture platforms |
| CN105154386B (zh) * | 2014-05-30 | 2018-04-24 | 中国人民解放军第二军医大学东方肝胆外科医院 | 人肝细胞长期维持和增殖传代培养的专用培养基和培养方法 |
| EP4177336A1 (en) * | 2014-12-19 | 2023-05-10 | Immusoft Corporation | B cells for in vivo delivery of therapeutic agents |
| KR101768581B1 (ko) * | 2015-08-13 | 2017-08-17 | 전북대학교 산학협력단 | 자발적 불멸화된 체세포의 단일 세포로부터 리프로그래밍 세포주의 제조방법 |
| AU2016338680B2 (en) | 2015-10-16 | 2022-11-17 | Fate Therapeutics, Inc. | Platform for the induction and maintenance of ground state pluripotency |
| CA3010764A1 (en) * | 2016-01-12 | 2017-07-20 | Lonza Walkersville, Inc. | Methods and vectors to produce vector free induced pluripotent stem cells |
| US10201540B2 (en) | 2016-03-02 | 2019-02-12 | Frequency Therapeutics, Inc. | Solubilized compositions for controlled proliferation of stem cells / generating inner ear hair cells using GSK3 inhibitors: I |
| US10213511B2 (en) | 2016-03-02 | 2019-02-26 | Frequency Therapeutics, Inc. | Thermoreversible compositions for administration of therapeutic agents |
| US20190060371A1 (en) * | 2016-03-02 | 2019-02-28 | Frequency Therapeutics, Inc. | Methods for controlled proliferation of vestibular stem cells / generating inner ear hair cells using wnt and tgfbeta-inhibition |
| US11185555B2 (en) | 2016-04-11 | 2021-11-30 | Noah James Harrison | Method to kill pathogenic microbes in a patient |
| WO2017217756A2 (ko) * | 2016-06-15 | 2017-12-21 | 한국생명공학연구원 | 자살유전자를 포함한 에피솜 벡터 및 이를 이용한 리프로그래밍 방법 |
| US11572545B2 (en) | 2016-06-16 | 2023-02-07 | Cedars-Sinai Medical Center | Efficient method for reprogramming blood to induced pluripotent stem cells |
| US10221395B2 (en) | 2016-06-16 | 2019-03-05 | Cedars-Sinai Medical Center | Efficient method for reprogramming blood to induced pluripotent stem cells |
| CN108148808B (zh) * | 2016-12-05 | 2020-12-11 | 同济大学 | 有助于诱导生成神经前体细胞的诱导培养基 |
| US11624056B2 (en) | 2017-01-19 | 2023-04-11 | Yipcell Inc. | Method for producing cartilage cells induced to be differentiated from stem cells |
| US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| CN110621323B (zh) * | 2017-05-02 | 2022-10-14 | 田边刚士 | 医药品组合物及化妆品组合物 |
| WO2018232079A1 (en) | 2017-06-14 | 2018-12-20 | Daley George Q | Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells by episomal plasmid gene transfer |
| CA3078734A1 (en) * | 2017-10-11 | 2019-04-18 | Fate Therapeutics, Inc. | Cellular reprogramming using temporal and transient plasmid vector expression system |
| WO2019106163A1 (en) * | 2017-12-01 | 2019-06-06 | Cellectis | Reprogramming of genetically engineered primary immune cells |
| JP7473209B2 (ja) * | 2018-01-29 | 2024-04-23 | 中国科学院動物研究所 | 細胞誘導の方法 |
| WO2020037326A1 (en) | 2018-08-17 | 2020-02-20 | Frequency Therapeutics, Inc. | Compositions and methods for generating hair cells by downregulating foxo |
| US11162071B2 (en) | 2018-08-17 | 2021-11-02 | Frequency Therapeutics, Inc. | Compositions and methods for generating hair cells by upregulating JAG-1 |
| WO2020074925A2 (en) * | 2018-10-12 | 2020-04-16 | Reneuron Limited | Induced pluripotent cell comprising a controllable transgene for conditional immortalisation |
| EP3946470A4 (en) * | 2019-04-05 | 2023-05-31 | Earli Inc. | IMPROVED METHODS AND COMPOSITIONS FOR SYNTHETIC BIOMARKERS |
| MX2022008648A (es) | 2020-01-23 | 2022-12-15 | The Children´S Medical Center Corp | Diferenciacion de celulas t sin estroma a partir de celulas madre pluripotentes humanas. |
| BR112022018092A2 (pt) | 2020-03-11 | 2022-11-22 | Bit Bio Ltd | Método de gerar células hepáticas |
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| CN117441010A (zh) | 2021-04-07 | 2024-01-23 | 世纪治疗股份有限公司 | 从诱导多能干细胞产生α-βT细胞的组合物和方法 |
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| WO2025101938A2 (en) | 2023-11-10 | 2025-05-15 | Century Therapeutics, Inc. | Genetically engineered cells having multi-transmembrane domain chimeric antigen receptors utilizing g protein-coupled receptor scaffolds, and uses thereof |
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| WO2025207798A1 (en) | 2024-03-26 | 2025-10-02 | Century Therapeutics, Inc. | Genetically engineered cells having anti-cd123 chimeric antigen receptors, and uses thereof |
| WO2025207795A1 (en) | 2024-03-26 | 2025-10-02 | Juno Therapeutics, Inc. | Genetically engineered cells having anti-cd33 / anti-cd123 chimeric antigen receptors, and uses thereof |
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| NL8200523A (nl) | 1982-02-11 | 1983-09-01 | Univ Leiden | Werkwijze voor het in vitro transformeren van planteprotoplasten met plasmide-dna. |
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- 2011-08-03 EP EP11815273.5A patent/EP2601289B1/en active Active
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| JP2013534140A (ja) | 2013-09-02 |
| EP2601289A4 (en) | 2014-01-22 |
| US20120058562A1 (en) | 2012-03-08 |
| EP2601289A2 (en) | 2013-06-12 |
| CA2806858A1 (en) | 2012-02-09 |
| WO2012018933A2 (en) | 2012-02-09 |
| WO2012018933A3 (en) | 2012-05-10 |
| EP2601289B1 (en) | 2017-07-12 |
| US20140349397A1 (en) | 2014-11-27 |
| US8765470B2 (en) | 2014-07-01 |
| JP5936218B2 (ja) | 2016-06-22 |
| JP2016039828A (ja) | 2016-03-24 |
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