CA2806858C - Reprogramming immortalized b cells - Google Patents

Reprogramming immortalized b cells Download PDF

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Publication number
CA2806858C
CA2806858C CA2806858A CA2806858A CA2806858C CA 2806858 C CA2806858 C CA 2806858C CA 2806858 A CA2806858 A CA 2806858A CA 2806858 A CA2806858 A CA 2806858A CA 2806858 C CA2806858 C CA 2806858C
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cells
pluripotent stem
induced pluripotent
reprogramming
immortalized
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French (fr)
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CA2806858A1 (en
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James Thomson
Deepika Rajesh
Sarah Jane Dickerson
Amanda Mack
Michael Miller
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Fujifilm Cellular Dynamics Inc
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Fujifilm Cellular Dynamics Inc
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CA2806858A 2010-08-04 2011-08-03 Reprogramming immortalized b cells Active CA2806858C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US37061510P 2010-08-04 2010-08-04
US61/370,615 2010-08-04
US201161441885P 2011-02-11 2011-02-11
US61/441,885 2011-02-11
PCT/US2011/046452 WO2012018933A2 (en) 2010-08-04 2011-08-03 Reprogramming immortalized b cells

Publications (2)

Publication Number Publication Date
CA2806858A1 CA2806858A1 (en) 2012-02-09
CA2806858C true CA2806858C (en) 2021-06-15

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CA2806858A Active CA2806858C (en) 2010-08-04 2011-08-03 Reprogramming immortalized b cells

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US (2) US8765470B2 (cg-RX-API-DMAC7.html)
EP (1) EP2601289B1 (cg-RX-API-DMAC7.html)
JP (2) JP5936218B2 (cg-RX-API-DMAC7.html)
CA (1) CA2806858C (cg-RX-API-DMAC7.html)
WO (1) WO2012018933A2 (cg-RX-API-DMAC7.html)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2548950T3 (en) 2009-06-05 2017-12-11 Cellular Dynamics Int Inc Reprogramming of T cells and hematopoietic cells
KR101774206B1 (ko) 2009-08-07 2017-09-04 고쿠리츠 다이가쿠 호진 교토 다이가쿠 유도된 다능성 줄기 세포의 효율적인 확립 방법
KR101975688B1 (ko) * 2010-12-22 2019-05-07 페이트 세러퓨틱스, 인코포레이티드 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼
CA2841165A1 (en) * 2011-07-11 2013-01-17 Cellular Dynamics International, Inc. Methods for cell reprogramming and genome engineering
CN102604894B (zh) * 2012-02-29 2014-07-30 中国科学院广州生物医药与健康研究院 用于制备神经干细胞的培养基及其用途
CA2874259C (en) 2012-05-23 2021-02-09 Kyoto University Highly efficient method for establishing induced pluripotent stem cell
US10240125B2 (en) 2013-03-14 2019-03-26 Immusoft Corporation Methods for in vitro memory B cell differentiation and transduction with VSV-G pseudotyped viral vectors
WO2014186766A1 (en) * 2013-05-17 2014-11-20 The Broad Institute, Inc. Reprogrammed cells and methods of production and use thereof
US10738323B2 (en) 2013-07-12 2020-08-11 Cedars-Sinai Medical Center Generation of induced pluripotent stem cells from normal human mammary epithelial cells
KR102670874B1 (ko) 2014-03-04 2024-05-31 페이트 세러퓨틱스, 인코포레이티드 개선된 재프로그래밍 방법 및 세포 배양 플랫폼
CN105154386B (zh) * 2014-05-30 2018-04-24 中国人民解放军第二军医大学东方肝胆外科医院 人肝细胞长期维持和增殖传代培养的专用培养基和培养方法
DK3234107T3 (da) * 2014-12-19 2022-10-17 Immusoft Corp B-celler til in vivo-levering af terapeutiske midler
KR101768581B1 (ko) * 2015-08-13 2017-08-17 전북대학교 산학협력단 자발적 불멸화된 체세포의 단일 세포로부터 리프로그래밍 세포주의 제조방법
EP3362570A4 (en) 2015-10-16 2019-03-20 Fate Therapeutics, Inc. PLATFORM FOR THE INDUCTION AND MAINTENANCE OF BASIC STATUS PLURIPOTENCE
CA3010764A1 (en) * 2016-01-12 2017-07-20 Lonza Walkersville, Inc. Methods and vectors to produce vector free induced pluripotent stem cells
JP2019507609A (ja) * 2016-03-02 2019-03-22 フリークエンシー セラピューティクス インコーポレイテッド WNTおよびTGF−β阻害を用いる、前庭幹細胞の制御された増殖/内耳有毛細胞の発生のための方法
US10201540B2 (en) 2016-03-02 2019-02-12 Frequency Therapeutics, Inc. Solubilized compositions for controlled proliferation of stem cells / generating inner ear hair cells using GSK3 inhibitors: I
US10213511B2 (en) 2016-03-02 2019-02-26 Frequency Therapeutics, Inc. Thermoreversible compositions for administration of therapeutic agents
US11185555B2 (en) 2016-04-11 2021-11-30 Noah James Harrison Method to kill pathogenic microbes in a patient
KR101957370B1 (ko) * 2016-06-15 2019-03-13 한국생명공학연구원 자살유전자를 포함한 에피솜 벡터 및 이를 이용한 리프로그래밍 방법
US10221395B2 (en) 2016-06-16 2019-03-05 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US11572545B2 (en) 2016-06-16 2023-02-07 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
CN108148808B (zh) * 2016-12-05 2020-12-11 同济大学 有助于诱导生成神经前体细胞的诱导培养基
US11624056B2 (en) 2017-01-19 2023-04-11 Yipcell Inc. Method for producing cartilage cells induced to be differentiated from stem cells
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
US20200147146A1 (en) * 2017-05-02 2020-05-14 Koji Tanabe Pharmaceutical composition and cosmetic composition
WO2018232079A1 (en) 2017-06-14 2018-12-20 Daley George Q Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells by episomal plasmid gene transfer
US12247226B2 (en) * 2017-10-11 2025-03-11 Fate Therapeutics, Inc. Cellular reprogramming using temporal and transient plasmid vector expression system
WO2019106163A1 (en) * 2017-12-01 2019-06-06 Cellectis Reprogramming of genetically engineered primary immune cells
JP7473209B2 (ja) * 2018-01-29 2024-04-23 中国科学院動物研究所 細胞誘導の方法
US11162071B2 (en) 2018-08-17 2021-11-02 Frequency Therapeutics, Inc. Compositions and methods for generating hair cells by upregulating JAG-1
US11617745B2 (en) 2018-08-17 2023-04-04 Frequency Therapeutics, Inc. Compositions and methods for generating hair cells by downregulating FOXO
CA3115892A1 (en) * 2018-10-12 2020-04-16 Reneuron Limited Induced pluripotent cell comprising a controllable transgene for conditional immortalisation
GB2617512B (en) * 2019-04-05 2023-12-27 Earli Inc Improved methods and compositions for synthetic biomarkers
KR20220130158A (ko) 2020-01-23 2022-09-26 더 칠드런스 메디칼 센터 코포레이션 인간 만능 줄기 세포로부터의 무-간질 t 세포 분화
BR112022018092A2 (pt) 2020-03-11 2022-11-22 Bit Bio Ltd Método de gerar células hepáticas
US11661459B2 (en) 2020-12-03 2023-05-30 Century Therapeutics, Inc. Artificial cell death polypeptide for chimeric antigen receptor and uses thereof
EP4255922A1 (en) 2020-12-03 2023-10-11 Century Therapeutics, Inc. Genetically engineered cells and uses thereof
WO2022216524A1 (en) 2021-04-07 2022-10-13 Century Therapeutics, Inc. Combined artificial cell death/reporter system polypeptide for chimeric antigen receptor cell and uses thereof
IL307358A (en) 2021-04-07 2023-11-01 Century Therapeutics Inc Compositions and methods for generating gamma-delta t cells from induced pluripotent stem cells
AU2022253891A1 (en) 2021-04-07 2023-08-24 Century Therapeutics, Inc. Gene transfer vectors and methods of engineering cells
AU2022255166A1 (en) 2021-04-07 2023-09-28 Century Therapeutics, Inc. Compositions and methods for generating alpha-beta t cells from induced pluripotent stem cells
KR20240100375A (ko) * 2021-10-20 2024-07-01 엑시르, 인코포레이티드 전-임상 평가를 위한 비-인간 영장류의 개별화된 게놈 어셈블리 및 유도 만능 줄기 세포 라인을 사용하기 위한 조성물 및 방법
US20250064934A1 (en) 2021-12-29 2025-02-27 Century Therapeutics, Inc. Genetically engineered cells having anti-cd19 / anti-cd22 chimeric antigen receptors, and uses thereof
EP4519319A1 (en) 2022-05-04 2025-03-12 Century Therapeutics, Inc. Cells engineered with an hla-e and hla-g transgene
CA3257424A1 (en) 2022-06-08 2023-12-14 Century Therapeutics, Inc. Genetically modified cells expressing variants of CD16 and NKG2D and their uses
WO2023240212A2 (en) 2022-06-08 2023-12-14 Century Therapeutics, Inc. Genetically engineered cells having anti-cd133 / anti-egfr chimeric antigen receptors, and uses thereof
CA3257650A1 (en) 2022-06-08 2023-12-14 Century Therapeutics, Inc. Immunoeffector cells derived from genetically modified, multipotential stem cells with membrane IL-12 and their uses
WO2024102838A1 (en) 2022-11-09 2024-05-16 Century Therapeutics, Inc. Engineered interleukin-7 receptors and uses thereof
EP4615876A2 (en) 2022-11-10 2025-09-17 Century Therapeutics, Inc. Genetically engineered cells having anti-nectin4 chimeric antigen receptors, and uses thereof
WO2025101938A2 (en) 2023-11-10 2025-05-15 Century Therapeutics, Inc. Genetically engineered cells having multi-transmembrane domain chimeric antigen receptors utilizing g protein-coupled receptor scaffolds, and uses thereof
WO2025106626A1 (en) 2023-11-15 2025-05-22 Century Therapeutics, Inc. Genetically engineered cells expressing c-x-c chemokine receptor type 4, and uses thereof
WO2025207795A1 (en) 2024-03-26 2025-10-02 Juno Therapeutics, Inc. Genetically engineered cells having anti-cd33 / anti-cd123 chimeric antigen receptors, and uses thereof
WO2025207798A1 (en) 2024-03-26 2025-10-02 Century Therapeutics, Inc. Genetically engineered cells having anti-cd123 chimeric antigen receptors, and uses thereof

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8200523A (nl) 1982-02-11 1983-09-01 Univ Leiden Werkwijze voor het in vitro transformeren van planteprotoplasten met plasmide-dna.
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4952500A (en) 1988-02-01 1990-08-28 University Of Georgia Research Foundation, Inc. Cloning systems for Rhodococcus and related bacteria
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
AU2515992A (en) 1991-08-20 1993-03-16 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
US5610042A (en) 1991-10-07 1997-03-11 Ciba-Geigy Corporation Methods for stable transformation of wheat
ATE398679T1 (de) 1992-07-07 2008-07-15 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US5702932A (en) 1992-07-20 1997-12-30 University Of Florida Microinjection methods to transform arthropods with exogenous DNA
BR9306802A (pt) 1992-07-27 1998-12-08 Pioneer Hi Bred Int Processo independente de genótipos para produção de planta de soja transgénica e processo de regeneração de plantas de soja a partir de nodos cotiledonais
DE4228457A1 (de) 1992-08-27 1994-04-28 Beiersdorf Ag Herstellung von heterodimerem PDGF-AB mit Hilfe eines bicistronischen Vektorsystems in Säugerzellen
GB9222888D0 (en) 1992-10-30 1992-12-16 British Tech Group Tomography
US5656610A (en) 1994-06-21 1997-08-12 University Of Southern California Producing a protein in a mammal by injection of a DNA-sequence into the tongue
FR2722208B1 (fr) 1994-07-05 1996-10-04 Inst Nat Sante Rech Med Nouveau site interne d'entree des ribosomes, vecteur le contenant et utilisation therapeutique
US5736524A (en) 1994-11-14 1998-04-07 Merck & Co.,. Inc. Polynucleotide tuberculosis vaccine
US5780448A (en) 1995-11-07 1998-07-14 Ottawa Civic Hospital Loeb Research DNA-based vaccination of fish
GB9807935D0 (en) 1998-04-14 1998-06-10 Univ Edinburgh Lineage specific cells and progenitor cells
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
US5945100A (en) 1996-07-31 1999-08-31 Fbp Corporation Tumor delivery vehicles
US5981274A (en) 1996-09-18 1999-11-09 Tyrrell; D. Lorne J. Recombinant hepatitis virus vectors
US5994624A (en) 1997-10-20 1999-11-30 Cotton Incorporated In planta method for the production of transgenic plants
AU729377B2 (en) 1997-10-23 2001-02-01 Asterias Biotherapeutics, Inc. Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
US6280718B1 (en) 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
US6458589B1 (en) 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
US7473555B2 (en) 2000-04-27 2009-01-06 Geron Corporation Protocols for making hepatocytes from embryonic stem cells
US7250294B2 (en) 2000-05-17 2007-07-31 Geron Corporation Screening small molecule drugs using neural cells differentiated from human embryonic stem cells
GB2379447B (en) 2000-05-17 2004-12-29 Geron Corp Neural progenitor cell populations
KR101073411B1 (ko) 2001-07-12 2011-10-17 제론 코포레이션 인간 다분화능 줄기 세포로부터 제조된 심근 세포 계통의 세포
US20030211603A1 (en) 2001-08-14 2003-11-13 Earp David J. Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells
IL162130A0 (en) 2001-12-07 2005-11-20 Robarts Res Inst Hematopoietic cells from human embryonic stem cells
CN1602351B (zh) 2001-12-07 2011-03-30 杰龙公司 源自人胚胎干细胞的胰岛细胞
CA2684022C (en) 2002-05-17 2014-09-23 Mount Sinai School Of Medicine Of New York University Mesoderm and definitive endoderm cell populations
DE10224242A1 (de) 2002-05-29 2003-12-11 Max Delbrueck Centrum Frog Prince, ein Transposonvektor für den Gentransfer bei Wirbeltieren
US7285415B2 (en) 2002-07-11 2007-10-23 The Regents Of The University Of California Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US7452718B2 (en) 2004-03-26 2008-11-18 Geron Corporation Direct differentiation method for making cardiomyocytes from human embryonic stem cells
US20080038820A1 (en) 2004-06-22 2008-02-14 Rudy-Reil Diane E Induction of pluripotent stem cells into mesodermal lineages
US7442548B2 (en) 2004-09-08 2008-10-28 Wisconsin Alumni Research Foundation Culturing human embryonic stem cells in medium containing pipecholic acid and gamma amino butyric acid
JP2008523823A (ja) 2004-12-22 2008-07-10 イーエス・セル・インターナショナル・プライヴェート・リミテッド ヒト胚性幹細胞の分化ならびにそれに由来する心筋細胞および心筋前駆細胞
WO2008005268A1 (en) 2006-06-30 2008-01-10 Schering Corporation Substituted piperidines that increase p53 activity and the uses thereof
AR061974A1 (es) 2006-07-14 2008-08-10 Novartis Ag Derivados de pirimidina como inhibidores de alk, composiciones farmaceuticas y procesos de obtencion
CN101641436A (zh) 2007-01-30 2010-02-03 佐治亚大学研究基金会 用于产生内胚层和中胚层细胞系及多能游走细胞(mmc)的早期中胚层细胞即稳定的中内胚层细胞群
MX352541B (es) 2007-04-07 2017-11-29 Whitehead Inst Biomedical Res Reprogramación de células somáticas.
CA2954948A1 (en) 2008-06-04 2009-12-10 Cellular Dynamics International, Inc. Methods for the production of ips cells using non-viral approach
JP2011525794A (ja) 2008-06-26 2011-09-29 国立大学法人大阪大学 iPS細胞の製造方法および製造キット
KR101773345B1 (ko) 2008-08-12 2017-08-31 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 iPS 세포의 생산 방법
WO2010027062A1 (ja) * 2008-09-04 2010-03-11 独立行政法人理化学研究所 B細胞由来iPS細胞およびその用途
CA2753208C (en) 2009-02-20 2018-08-21 Cellular Dynamics International, Inc. Methods and compositions for the differentiation of stem cells
EP2401364B1 (en) 2009-02-27 2015-04-22 Cellular Dynamics International, Inc. Differentiation of pluripotent cells
DK2548950T3 (en) * 2009-06-05 2017-12-11 Cellular Dynamics Int Inc Reprogramming of T cells and hematopoietic cells
EP4364797A3 (en) 2009-10-19 2024-07-10 FUJIFILM Cellular Dynamics, Inc. Cardiomyocyte production
JP5898086B2 (ja) 2009-11-04 2016-04-06 セルラー ダイナミクス インターナショナル, インコーポレイテッド 化学物質を用いるエピソームリプログラミング
CN102597524B (zh) * 2009-11-06 2015-11-25 三菱电机株式会社 热泵装置、双级压缩机及热泵装置的运转方法
CA2796251C (en) 2010-04-13 2019-05-14 Cellular Dynamics International, Inc. Hepatocyte production by forward programming
WO2012021632A2 (en) * 2010-08-10 2012-02-16 The Johns Hopkins University Generation and use of pluripotent stem cells

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WO2012018933A3 (en) 2012-05-10
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US20140349397A1 (en) 2014-11-27
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CA2806858A1 (en) 2012-02-09
US8765470B2 (en) 2014-07-01

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