CA2742594C - Modified type a dna polymerases - Google Patents

Modified type a dna polymerases Download PDF

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Publication number
CA2742594C
CA2742594C CA2742594A CA2742594A CA2742594C CA 2742594 C CA2742594 C CA 2742594C CA 2742594 A CA2742594 A CA 2742594A CA 2742594 A CA2742594 A CA 2742594A CA 2742594 C CA2742594 C CA 2742594C
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CA
Canada
Prior art keywords
seq
dna polymerase
polymerase
taq dna
modified
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Active
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CA2742594A
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English (en)
French (fr)
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CA2742594A1 (en
Inventor
William Bourn
Maryke Appel
Gavin Rush
John Foskett
Paul Mcewan
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Kapa Biosystems Inc
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Kapa Biosystems Inc
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Publication of CA2742594A1 publication Critical patent/CA2742594A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)
    • G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)
    • G01N2333/9126—DNA-directed DNA polymerase (2.7.7.7)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Ecology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2742594A 2008-11-03 2009-11-03 Modified type a dna polymerases Active CA2742594C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11087708P 2008-11-03 2008-11-03
US61/110,877 2008-11-03
PCT/US2009/063167 WO2010062777A2 (en) 2008-11-03 2009-11-03 Modified type a dna polymerases

Publications (2)

Publication Number Publication Date
CA2742594A1 CA2742594A1 (en) 2010-06-03
CA2742594C true CA2742594C (en) 2020-03-24

Family

ID=42226350

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2742594A Active CA2742594C (en) 2008-11-03 2009-11-03 Modified type a dna polymerases

Country Status (7)

Country Link
US (4) US10457968B2 (enExample)
EP (2) EP3101124B1 (enExample)
JP (2) JP5809059B2 (enExample)
CN (1) CN102245761A (enExample)
CA (1) CA2742594C (enExample)
ES (1) ES2742800T3 (enExample)
WO (1) WO2010062777A2 (enExample)

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EP3101124B1 (en) 2008-11-03 2019-06-19 Kapa Biosystems, Inc. Modified type a dna polymerases
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WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
WO2013008898A1 (ja) * 2011-07-12 2013-01-17 国立大学法人九州大学 新規dnaポリメラーゼ
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
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EP2882852B1 (en) * 2012-08-08 2017-03-22 Universität Konstanz New dna polymerases with increased substrate scope
WO2014062848A1 (en) * 2012-10-16 2014-04-24 Dna Polymerase Technology, Inc. Inhibition-resistant polymerases
US9758773B2 (en) * 2014-02-14 2017-09-12 Agilent Technologies, Inc. Thermostable type-A DNA polymerase mutant with increased resistance to inhibitors in blood
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WO2016100438A2 (en) 2014-12-16 2016-06-23 Life Technologies Corporation Polymerase compositions and methods of making and using same
CN107257854B (zh) 2015-02-02 2022-02-15 豪夫迈·罗氏有限公司 聚合酶变体
US11091745B2 (en) 2015-05-12 2021-08-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
US10526588B2 (en) * 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
JP6963238B2 (ja) * 2015-11-27 2021-11-05 国立大学法人九州大学 Ｄｎａポリメラーゼ変異体
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CN107299091B (zh) * 2017-08-17 2021-07-30 苏州新海生物科技股份有限公司 一种突变型a型dna聚合酶及其编码基因和应用
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CN113316636B (zh) * 2018-12-20 2023-04-28 深圳华大生命科学研究院 酶活性提高的dna聚合酶及其应用
US11168312B2 (en) * 2019-03-10 2021-11-09 Abclonal Science, Inc. Mutant taq polymerase for amplification in increased salt concentration or body fluids
US10865441B2 (en) * 2019-03-13 2020-12-15 Abclonal Science, Inc. Mutant Taq polymerase for faster amplification
US11613738B2 (en) * 2019-05-14 2023-03-28 Abclonal Science, Inc. Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye
CN110684752B (zh) * 2019-10-08 2020-09-29 南京诺唯赞生物科技股份有限公司 一种耐受性提高的突变型Taq DNA聚合酶及其制备方法和应用
CN110747183B (zh) * 2019-10-29 2020-10-23 南京诺唯赞生物科技股份有限公司 一种Taq DNA聚合酶突变体及其应用
CN111690626B (zh) * 2020-07-02 2021-03-26 南京诺唯赞生物科技股份有限公司 一种融合型Taq DNA聚合酶及其制备方法和应用
CN114480328B (zh) * 2020-10-26 2024-01-16 厦门大学 一种Taq DNA聚合酶突变体
CN118339283A (zh) * 2021-12-24 2024-07-12 深圳华大生命科学研究院 Dna聚合酶突变体及其应用
CN117467640B (zh) * 2023-09-28 2024-12-13 郑州玛特瑞斯生物科技有限公司 突变型Taq DNA聚合酶及其应用

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Publication number Publication date
JP5809059B2 (ja) 2015-11-10
EP3101124B1 (en) 2019-06-19
US20110281305A1 (en) 2011-11-17
US10457968B2 (en) 2019-10-29
US20210180102A1 (en) 2021-06-17
US11603547B2 (en) 2023-03-14
JP5976895B2 (ja) 2016-08-24
EP3101124A1 (en) 2016-12-07
WO2010062777A2 (en) 2010-06-03
EP2352819A2 (en) 2011-08-10
CA2742594A1 (en) 2010-06-03
US10961555B2 (en) 2021-03-30
EP2352819A4 (en) 2013-01-09
JP2015171384A (ja) 2015-10-01
CN102245761A (zh) 2011-11-16
US20170051326A1 (en) 2017-02-23
WO2010062777A3 (en) 2010-08-26
US20190100783A1 (en) 2019-04-04
ES2742800T3 (es) 2020-02-17
JP2012507987A (ja) 2012-04-05

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