CA2696528A1 - Method and device for fixing/stabilising a sample - Google Patents
Method and device for fixing/stabilising a sample Download PDFInfo
- Publication number
- CA2696528A1 CA2696528A1 CA 2696528 CA2696528A CA2696528A1 CA 2696528 A1 CA2696528 A1 CA 2696528A1 CA 2696528 CA2696528 CA 2696528 CA 2696528 A CA2696528 A CA 2696528A CA 2696528 A1 CA2696528 A1 CA 2696528A1
- Authority
- CA
- Canada
- Prior art keywords
- sample
- composition
- fixing
- permeable container
- container
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 44
- 230000003019 stabilising effect Effects 0.000 title abstract description 5
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 11
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- 239000000203 mixture Substances 0.000 claims description 66
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 45
- 230000000087 stabilizing effect Effects 0.000 claims description 29
- 239000003795 chemical substances by application Substances 0.000 claims description 19
- 239000000654 additive Substances 0.000 claims description 18
- 229920005862 polyol Polymers 0.000 claims description 18
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 16
- 150000003077 polyols Chemical class 0.000 claims description 14
- 230000000996 additive effect Effects 0.000 claims description 13
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims description 12
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 12
- 239000000872 buffer Substances 0.000 claims description 12
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 11
- 239000008096 xylene Substances 0.000 claims description 11
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- 239000012620 biological material Substances 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 9
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000002202 Polyethylene glycol Substances 0.000 claims description 8
- WERYXYBDKMZEQL-UHFFFAOYSA-N butane-1,4-diol Chemical compound OCCCCO WERYXYBDKMZEQL-UHFFFAOYSA-N 0.000 claims description 8
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- 239000002904 solvent Substances 0.000 claims description 8
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- 230000015556 catabolic process Effects 0.000 claims description 7
- 238000006731 degradation reaction Methods 0.000 claims description 7
- 108020004707 nucleic acids Proteins 0.000 claims description 7
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- 150000007523 nucleic acids Chemical class 0.000 claims description 7
- 239000003381 stabilizer Substances 0.000 claims description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 6
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- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 6
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- JFDZBHWFFUWGJE-UHFFFAOYSA-N benzonitrile Chemical compound N#CC1=CC=CC=C1 JFDZBHWFFUWGJE-UHFFFAOYSA-N 0.000 claims description 6
- 230000003196 chaotropic effect Effects 0.000 claims description 6
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 claims description 6
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- ARXKVVRQIIOZGF-UHFFFAOYSA-N 1,2,4-butanetriol Chemical compound OCCC(O)CO ARXKVVRQIIOZGF-UHFFFAOYSA-N 0.000 claims description 4
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- FPZWZCWUIYYYBU-UHFFFAOYSA-N 2-(2-ethoxyethoxy)ethyl acetate Chemical compound CCOCCOCCOC(C)=O FPZWZCWUIYYYBU-UHFFFAOYSA-N 0.000 claims description 4
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- FXHOOIRPVKKKFG-UHFFFAOYSA-N N,N-Dimethylacetamide Chemical compound CN(C)C(C)=O FXHOOIRPVKKKFG-UHFFFAOYSA-N 0.000 claims description 4
- 108091005461 Nucleic proteins Proteins 0.000 claims description 4
- SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 4
- RDOXTESZEPMUJZ-UHFFFAOYSA-N anisole Chemical compound COC1=CC=CC=C1 RDOXTESZEPMUJZ-UHFFFAOYSA-N 0.000 claims description 4
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 4
- 239000003638 chemical reducing agent Substances 0.000 claims description 4
- 239000008139 complexing agent Substances 0.000 claims description 4
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 claims description 4
- 239000003599 detergent Substances 0.000 claims description 4
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims description 4
- MTZQAGJQAFMTAQ-UHFFFAOYSA-N ethyl benzoate Chemical compound CCOC(=O)C1=CC=CC=C1 MTZQAGJQAFMTAQ-UHFFFAOYSA-N 0.000 claims description 4
- 239000003112 inhibitor Substances 0.000 claims description 4
- QPJVMBTYPHYUOC-UHFFFAOYSA-N methyl benzoate Chemical compound COC(=O)C1=CC=CC=C1 QPJVMBTYPHYUOC-UHFFFAOYSA-N 0.000 claims description 4
- AJFDBNQQDYLMJN-UHFFFAOYSA-N n,n-diethylacetamide Chemical compound CCN(CC)C(C)=O AJFDBNQQDYLMJN-UHFFFAOYSA-N 0.000 claims description 4
- 125000003729 nucleotide group Chemical group 0.000 claims description 4
- FDPIMTJIUBPUKL-UHFFFAOYSA-N pentan-3-one Chemical compound CCC(=O)CC FDPIMTJIUBPUKL-UHFFFAOYSA-N 0.000 claims description 4
- 230000035699 permeability Effects 0.000 claims description 4
- 239000003755 preservative agent Substances 0.000 claims description 4
- URAYPUMNDPQOKB-UHFFFAOYSA-N triacetin Chemical compound CC(=O)OCC(OC(C)=O)COC(C)=O URAYPUMNDPQOKB-UHFFFAOYSA-N 0.000 claims description 4
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical compound ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 claims description 3
- 150000002009 diols Chemical class 0.000 claims description 3
- 235000011187 glycerol Nutrition 0.000 claims description 3
- 238000002844 melting Methods 0.000 claims description 3
- 230000008018 melting Effects 0.000 claims description 3
- MGDNHIJGIWHQBL-UHFFFAOYSA-N n-ethyl-n-methylacetamide Chemical compound CCN(C)C(C)=O MGDNHIJGIWHQBL-UHFFFAOYSA-N 0.000 claims description 3
- DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 claims description 2
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 claims description 2
- UOCLXMDMGBRAIB-UHFFFAOYSA-N 1,1,1-trichloroethane Chemical compound CC(Cl)(Cl)Cl UOCLXMDMGBRAIB-UHFFFAOYSA-N 0.000 claims description 2
- YAXKTBLXMTYWDQ-UHFFFAOYSA-N 1,2,3-butanetriol Chemical compound CC(O)C(O)CO YAXKTBLXMTYWDQ-UHFFFAOYSA-N 0.000 claims description 2
- 229940015975 1,2-hexanediol Drugs 0.000 claims description 2
- YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 claims description 2
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 claims description 2
- HBFBFJVRBIGLND-UHFFFAOYSA-N 2,2-bis(hydroxymethyl)butane-1,4-diol Chemical compound OCCC(CO)(CO)CO HBFBFJVRBIGLND-UHFFFAOYSA-N 0.000 claims description 2
- LCZVSXRMYJUNFX-UHFFFAOYSA-N 2-[2-(2-hydroxypropoxy)propoxy]propan-1-ol Chemical compound CC(O)COC(C)COC(C)CO LCZVSXRMYJUNFX-UHFFFAOYSA-N 0.000 claims description 2
- XAWCLWKTUKMCMO-UHFFFAOYSA-N 2-nitroethylbenzene Chemical compound [O-][N+](=O)CCC1=CC=CC=C1 XAWCLWKTUKMCMO-UHFFFAOYSA-N 0.000 claims description 2
- AHHQDHCTHYTBSV-UHFFFAOYSA-N 3-methylpentane-1,3,5-triol Chemical compound OCCC(O)(C)CCO AHHQDHCTHYTBSV-UHFFFAOYSA-N 0.000 claims description 2
- 239000005711 Benzoic acid Substances 0.000 claims description 2
- UDSFAEKRVUSQDD-UHFFFAOYSA-N Dimethyl adipate Chemical compound COC(=O)CCCCC(=O)OC UDSFAEKRVUSQDD-UHFFFAOYSA-N 0.000 claims description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 2
- 239000004348 Glyceryl diacetate Substances 0.000 claims description 2
- WRQNANDWMGAFTP-UHFFFAOYSA-N Methylacetoacetic acid Chemical compound COC(=O)CC(C)=O WRQNANDWMGAFTP-UHFFFAOYSA-N 0.000 claims description 2
- SUAKHGWARZSWIH-UHFFFAOYSA-N N,N‐diethylformamide Chemical compound CCN(CC)C=O SUAKHGWARZSWIH-UHFFFAOYSA-N 0.000 claims description 2
- ALQSHHUCVQOPAS-UHFFFAOYSA-N Pentane-1,5-diol Chemical compound OCCCCCO ALQSHHUCVQOPAS-UHFFFAOYSA-N 0.000 claims description 2
- XSEQBUVOXWTHGU-UHFFFAOYSA-N Pieristoxin G Natural products CC1(O)CC2(C(C(O)C3(O)C4(C)C)O)C(O)C1CCC2(O)C(C)(O)C3C1C4O1 XSEQBUVOXWTHGU-UHFFFAOYSA-N 0.000 claims description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 claims description 2
- KXKVLQRXCPHEJC-UHFFFAOYSA-N acetic acid trimethyl ester Natural products COC(C)=O KXKVLQRXCPHEJC-UHFFFAOYSA-N 0.000 claims description 2
- 239000012345 acetylating agent Substances 0.000 claims description 2
- 150000001298 alcohols Chemical class 0.000 claims description 2
- 239000002168 alkylating agent Substances 0.000 claims description 2
- 229940100198 alkylating agent Drugs 0.000 claims description 2
- 150000001450 anions Chemical class 0.000 claims description 2
- 150000004945 aromatic hydrocarbons Chemical class 0.000 claims description 2
- 235000010233 benzoic acid Nutrition 0.000 claims description 2
- BMRWNKZVCUKKSR-UHFFFAOYSA-N butane-1,2-diol Chemical compound CCC(O)CO BMRWNKZVCUKKSR-UHFFFAOYSA-N 0.000 claims description 2
- OWBTYPJTUOEWEK-UHFFFAOYSA-N butane-2,3-diol Chemical compound CC(O)C(C)O OWBTYPJTUOEWEK-UHFFFAOYSA-N 0.000 claims description 2
- 125000004432 carbon atom Chemical group C* 0.000 claims description 2
- 150000003857 carboxamides Chemical class 0.000 claims description 2
- 150000001735 carboxylic acids Chemical class 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- IEJIGPNLZYLLBP-UHFFFAOYSA-N dimethyl carbonate Chemical compound COC(=O)OC IEJIGPNLZYLLBP-UHFFFAOYSA-N 0.000 claims description 2
- BDUPRNVPXOHWIL-UHFFFAOYSA-N dimethyl sulfite Chemical compound COS(=O)OC BDUPRNVPXOHWIL-UHFFFAOYSA-N 0.000 claims description 2
- SZXQTJUDPRGNJN-UHFFFAOYSA-N dipropylene glycol Chemical compound OCCCOCCCO SZXQTJUDPRGNJN-UHFFFAOYSA-N 0.000 claims description 2
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- XYIBRDXRRQCHLP-UHFFFAOYSA-N ethyl acetoacetate Chemical compound CCOC(=O)CC(C)=O XYIBRDXRRQCHLP-UHFFFAOYSA-N 0.000 claims description 2
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- XYXCXCJKZRDVPU-UHFFFAOYSA-N hexane-1,2,3-triol Chemical compound CCCC(O)C(O)CO XYXCXCJKZRDVPU-UHFFFAOYSA-N 0.000 claims description 2
- UFAPLAOEQMMKJA-UHFFFAOYSA-N hexane-1,2,5-triol Chemical compound CC(O)CCC(O)CO UFAPLAOEQMMKJA-UHFFFAOYSA-N 0.000 claims description 2
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- PUXPSUBLYDOJNF-UHFFFAOYSA-N hexane-2,3,5-triol Chemical compound CC(O)CC(O)C(C)O PUXPSUBLYDOJNF-UHFFFAOYSA-N 0.000 claims description 2
- QCIYAEYRVFUFAP-UHFFFAOYSA-N hexane-2,3-diol Chemical compound CCCC(O)C(C)O QCIYAEYRVFUFAP-UHFFFAOYSA-N 0.000 claims description 2
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- UZKWTJUDCOPSNM-UHFFFAOYSA-N methoxybenzene Substances CCCCOC=C UZKWTJUDCOPSNM-UHFFFAOYSA-N 0.000 claims description 2
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20070113714 EP2027922A1 (de) | 2007-08-02 | 2007-08-02 | Verfahren und Vorrichtung für das Fixieren/Stabilisieren einer Probe |
| EP07113714.5 | 2007-08-02 | ||
| PCT/EP2008/060132 WO2009016255A1 (de) | 2007-08-02 | 2008-08-01 | Verfahren und vorrichtung für das fixieren/stabilisieren einer probe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2696528A1 true CA2696528A1 (en) | 2009-02-05 |
| CA2696528C CA2696528C (en) | 2016-02-23 |
Family
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| Application Number | Title | Priority Date | Filing Date |
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| CA2694050A Active CA2694050C (en) | 2007-08-02 | 2008-08-01 | Method and device for fixing/stabilising a sample |
| CA2696528A Active CA2696528C (en) | 2007-08-02 | 2008-08-01 | Method and device for fixing/stabilising a sample |
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| CA2694050A Active CA2694050C (en) | 2007-08-02 | 2008-08-01 | Method and device for fixing/stabilising a sample |
Country Status (7)
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| US (2) | US9029138B2 (3Den) |
| EP (3) | EP2027922A1 (3Den) |
| JP (2) | JP5226785B2 (3Den) |
| CN (2) | CN101778672B (3Den) |
| AU (2) | AU2008281721B2 (3Den) |
| CA (2) | CA2694050C (3Den) |
| WO (2) | WO2009016254A1 (3Den) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| FR2948456B1 (fr) * | 2009-07-22 | 2011-09-30 | Reactifs Ral | Composition de fixation cytologique ou histologique et procedes de coloration |
| EP2468862A1 (en) * | 2010-12-23 | 2012-06-27 | Qiagen GmbH | Method and kit for processing wax-embedded biological samples |
| CN102251024B (zh) * | 2011-04-21 | 2013-01-09 | 中国海洋大学 | 一种激光显微切割染色体标本制作液及其应用 |
| CN107421796B (zh) | 2011-06-17 | 2021-05-04 | 罗氏血液诊断股份有限公司 | 用于生物样品的组织加工的溶液 |
| EP2741676A1 (en) * | 2011-08-09 | 2014-06-18 | Cook General Biotechnology LLC | Vial useable in tissue extraction procedures |
| EP2761020B1 (en) * | 2011-09-26 | 2018-06-13 | Qiagen GmbH | Stabilisation and isolation of extracellular nucleic acids |
| US11021733B2 (en) | 2011-09-26 | 2021-06-01 | Qiagen Gmbh | Stabilization and isolation of extracellular nucleic acids |
| AU2012314515B2 (en) | 2011-09-26 | 2018-03-15 | Preanalytix Gmbh | Stabilisation and isolation of extracellular nucleic acids |
| US10724074B2 (en) | 2012-09-25 | 2020-07-28 | Qiagen Gmbh | Stabilisation of biological samples |
| JP6381628B2 (ja) | 2013-03-18 | 2018-08-29 | キアゲン ゲーエムベーハー | 生物試料の安定化 |
| EP2976425B1 (en) | 2013-03-18 | 2019-11-06 | Qiagen GmbH | Stabilization and isolation of extracellular nucleic acids |
| CN104062160A (zh) * | 2014-06-29 | 2014-09-24 | 浙江大学 | 一种电镜生物样品批量组织处理装置及应用 |
| CN104673717B (zh) * | 2015-02-12 | 2017-11-28 | 中国医科大学 | 一种生物组织rna保护试剂及其制备方法和应用 |
| AU2016349644B2 (en) | 2015-11-06 | 2022-11-24 | Ventana Medical Systems, Inc. | Representative diagnostics |
| US11203777B2 (en) | 2015-11-20 | 2021-12-21 | Qiagen Gmbh | Method of preparing sterilized compositions for stabilization of extracellular nucleic acids |
| US20210018408A1 (en) * | 2017-12-29 | 2021-01-21 | Hoffmann-La Roche Inc. | Tissue sample preparation system |
| CN111397998A (zh) * | 2020-04-26 | 2020-07-10 | 中烨(山东)检验检测有限公司 | 一种用于组织细胞切片的染色液 |
| EP4153064A1 (en) * | 2020-07-16 | 2023-03-29 | Henderson, Davin | Kits and methods for diagnosing chronic wasting disease |
| KR102563528B1 (ko) * | 2021-02-23 | 2023-08-08 | (주)코아바이오텍 | 자궁 경부암 액상 세포 검사용 솔루션 조성물 |
| JPWO2022191336A1 (3Den) * | 2021-03-12 | 2022-09-15 |
Family Cites Families (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3515128A (en) * | 1967-12-11 | 1970-06-02 | Bernard F Mcevoy | Skin biopsy punch |
| US3949747A (en) * | 1974-10-03 | 1976-04-13 | Hevesy William K | Biopsy set |
| DE2903760C2 (de) * | 1979-02-01 | 1981-06-19 | Christophers, Enno, Prof. Dr. | Verschließbare Gewebestanze zur Entnahme von Gewebebiopsien aus Geweben |
| JPS59170249U (ja) * | 1983-04-28 | 1984-11-14 | トヨタ自動車株式会社 | 分析用試料サンプリング器具 |
| DE3501061A1 (de) | 1985-01-15 | 1986-07-17 | Bernhard Dr. 7801 Buchenbach Wolf | Vorrichtung zum entnehmen und fixieren von gewebeproben |
| US4946669A (en) * | 1987-10-09 | 1990-08-07 | Siegfried Barry A | Histological fixatives |
| US5104640A (en) | 1989-03-17 | 1992-04-14 | Wescor, Inc. | Fixative composition for fixing blood smears to slides |
| DE4019182A1 (de) * | 1989-06-24 | 1991-01-03 | Hermann Julius Dr Berger | Verfahren zum impraegnieren von gewebeproben in paraffin |
| US5089288A (en) * | 1989-06-24 | 1992-02-18 | Berger Hermann J | Method for impregnating tissue samples in paraffin |
| US5267572A (en) * | 1990-11-20 | 1993-12-07 | Bucalo Brian D | Biopsy instrument with tissue specimen retaining and retrieval device |
| US5635045A (en) * | 1993-05-20 | 1997-06-03 | Alam; Aftab | Apparatus for, and a method of, electroelution isolation of biomolecules and recovering biomolecules after elution |
| DE69433477T2 (de) * | 1993-09-20 | 2004-09-16 | Boston Scientific Corp., Natick | Vorrichtung zur mehrfach-entnahme von biopsie-proben |
| JP2602789B2 (ja) * | 1993-11-12 | 1997-04-23 | 篠 純子 | 組織標本作製用の脱水剤を置換するための脂溶性置換剤及び前記脂溶性置換剤を用いた組織標本作製方法 |
| US5827199A (en) * | 1996-06-24 | 1998-10-27 | Alexander; A. Melvin | Biopsy punch apparatus |
| JP4175675B2 (ja) * | 1996-11-13 | 2008-11-05 | オリンパス株式会社 | 連続生検具 |
| US5871454A (en) * | 1997-04-22 | 1999-02-16 | Majlessi; Heshmat | Percutaneous excisional biopsy device |
| JP2978847B2 (ja) * | 1997-08-01 | 1999-11-15 | 株式会社 ハードティッシュ | 含水土質試料の樹脂包埋方法と器具 |
| US5980468A (en) * | 1997-09-22 | 1999-11-09 | Zimmon Scientific Corporation | Apparatus and method for serial collection storage and processing of biopsy specimens |
| DE19820466C2 (de) * | 1998-05-07 | 2002-06-13 | Fraunhofer Ges Forschung | Vorrichtung und Verfahren zur gezielten Beaufschlagung einer biologischen Probe mit Schallwellen |
| US6440373B1 (en) * | 1999-10-15 | 2002-08-27 | Bel-Art Products, Inc. | Device for collecting and storing samples |
| US6337189B1 (en) * | 2000-02-08 | 2002-01-08 | Streck Laboratories, Inc. | Fixative system, method and composition for biological testing |
| US6468227B2 (en) * | 2000-03-17 | 2002-10-22 | Zimmon Science Corporation | Device for performing a medical procedure |
| EP1360509A1 (en) * | 2000-12-20 | 2003-11-12 | DSM IP Assets B.V. | Preparation of fluid samples by applying pressure |
| EP1262758A1 (de) * | 2001-05-25 | 2002-12-04 | Leica Mikrosysteme GmbH | Behälter und Vorrichtung zur Gewebepräparation für die Gewebeeinbettung |
| US6458598B1 (en) * | 2001-08-13 | 2002-10-01 | Dako A/S | Process for preparing and presenting a tissue sample for histological study |
| ES2636974T3 (es) | 2001-10-12 | 2017-10-10 | Becton, Dickinson And Company | Aparato de tipo cesta para el transporte de muestras biológicas |
| AU2002340158B8 (en) | 2001-10-12 | 2009-06-11 | Becton, Dickinson And Company | Method and apparatus for transporting biological samples |
| DE10154843A1 (de) * | 2001-11-08 | 2003-05-28 | Microm Int Gmbh | Verfahren und Vorrichtung zur Kennzeichnung von Objektträgern für mikrotomierte Gewebeproben und deren Bearbeitung |
| US7138226B2 (en) * | 2002-05-10 | 2006-11-21 | The University Of Miami | Preservation of RNA and morphology in cells and tissues |
| EP1530416A4 (en) * | 2002-07-30 | 2005-10-19 | Id Pty Ltd Ag | SAMPLING DEVICE |
| DK1545775T3 (da) * | 2002-09-26 | 2010-10-11 | Biopath Automation Llc | Kassette og indlejringsindretning, arrangeringsapparater og fremgangsmåder til håndtering af vævsprøver |
| JP3877213B2 (ja) * | 2003-02-07 | 2007-02-07 | 康彦 北山 | アレイブロック作成方法とこれに使用される組織くりぬき装置 |
| ATE285070T1 (de) * | 2003-03-05 | 2005-01-15 | Milestone Srl | Fixierungslösung |
| CA2849506C (en) * | 2003-10-17 | 2016-05-10 | Biopath Automation, L.L.C. | Cassette for handling and holding tissue samples during processing, embedding and microtome procedures, and methods therefor |
| US7445603B2 (en) | 2004-05-12 | 2008-11-04 | Zkz Science Corp. | Apparatus for removable distal internal cassette for in situ fixation and specimen processing with serial collection and storage of biopsy specimens |
| JP2006038847A (ja) * | 2004-06-22 | 2006-02-09 | Tadafumi Kawamoto | 凍結薄切片を粘着支持するのに適した粘着性プラスチックフィルム |
| JP3106269U (ja) * | 2004-06-29 | 2004-12-16 | 富士レビオ株式会社 | 生物試料採取具 |
| JP2006162489A (ja) * | 2004-12-09 | 2006-06-22 | Niigata Univ | ティッシュマイクロアレイの作製方法及びその作製キット |
| US20090124928A1 (en) * | 2005-01-21 | 2009-05-14 | Zimmon David S | Apparatus for Circumferential Suction Step Multibiopsy of the Esophagus or Other Luminal Structure with Serial Collection, Storage and Processing of Biopsy Specimens within a Removable Distal Cassette for In Situ Analysis |
| KR100679714B1 (ko) * | 2005-02-07 | 2007-02-07 | 재단법인서울대학교산학협력재단 | 3차원 구조의 마이크로 스파이크 및 그 제조 방법 |
| DE102005041781A1 (de) * | 2005-09-01 | 2007-03-08 | Leica Microsystems Nussloch Gmbh | Vorrichtung zur Herstellung von Gewebearrays |
| US7927564B2 (en) * | 2005-12-19 | 2011-04-19 | Leica Biosystems Richmond, Inc. | Histological specimen cassette |
| US8283134B2 (en) * | 2005-12-27 | 2012-10-09 | Kyoto University | Cassette for fixing, embedding and slicing biological tissues and method of using the cassette |
| EP1804045B1 (de) * | 2005-12-30 | 2014-03-12 | QIAGEN GmbH | Ein Verfahren und ein Kit zur Behandlung einer biologischen Probe |
| EP1965190A1 (en) * | 2007-02-27 | 2008-09-03 | Qiagen GmbH | Fixation of a biological sample |
| GB0714351D0 (en) | 2007-07-24 | 2007-09-05 | Home Office | A Sampling device |
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2007
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2008
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- 2008-08-01 JP JP2010518691A patent/JP5226785B2/ja active Active
- 2008-08-01 WO PCT/EP2008/060128 patent/WO2009016254A1/de active Application Filing
- 2008-08-01 US US12/671,369 patent/US9029138B2/en active Active
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Also Published As
| Publication number | Publication date |
|---|---|
| EP2173489A1 (de) | 2010-04-14 |
| CA2694050C (en) | 2015-07-14 |
| US20100255571A1 (en) | 2010-10-07 |
| CN101778672A (zh) | 2010-07-14 |
| JP2010535332A (ja) | 2010-11-18 |
| US9029138B2 (en) | 2015-05-12 |
| WO2009016254A1 (de) | 2009-02-05 |
| CN101778673B (zh) | 2013-10-23 |
| EP2027922A1 (de) | 2009-02-25 |
| EP2173489B1 (de) | 2015-03-11 |
| AU2008281721A1 (en) | 2009-02-05 |
| JP5226785B2 (ja) | 2013-07-03 |
| AU2008281721B2 (en) | 2014-02-06 |
| EP2175996A1 (de) | 2010-04-21 |
| EP2175996B1 (de) | 2015-10-14 |
| CA2694050A1 (en) | 2009-02-05 |
| AU2008281720A1 (en) | 2009-02-05 |
| AU2008281720B2 (en) | 2013-11-14 |
| US8673241B2 (en) | 2014-03-18 |
| CA2696528C (en) | 2016-02-23 |
| CN101778673A (zh) | 2010-07-14 |
| JP5265679B2 (ja) | 2013-08-14 |
| US20100267081A1 (en) | 2010-10-21 |
| JP2011511926A (ja) | 2011-04-14 |
| WO2009016255A1 (de) | 2009-02-05 |
| CN101778672B (zh) | 2013-02-27 |
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