CA2686597C - System and method for the automated extraction of nucleic acids - Google Patents
System and method for the automated extraction of nucleic acids Download PDFInfo
- Publication number
- CA2686597C CA2686597C CA2686597A CA2686597A CA2686597C CA 2686597 C CA2686597 C CA 2686597C CA 2686597 A CA2686597 A CA 2686597A CA 2686597 A CA2686597 A CA 2686597A CA 2686597 C CA2686597 C CA 2686597C
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acids
- plate
- reagent
- cavities
- samples
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 208
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 208
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 208
- 238000000034 method Methods 0.000 title claims abstract description 141
- 238000000605 extraction Methods 0.000 title claims abstract description 35
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 223
- 230000008569 process Effects 0.000 claims abstract description 131
- 239000012530 fluid Substances 0.000 claims abstract description 81
- 238000012545 processing Methods 0.000 claims abstract description 32
- 239000000203 mixture Substances 0.000 claims abstract description 25
- 238000002156 mixing Methods 0.000 claims abstract description 11
- 239000002699 waste material Substances 0.000 claims description 32
- 238000005406 washing Methods 0.000 claims description 17
- 238000012546 transfer Methods 0.000 claims description 4
- 238000011068 loading method Methods 0.000 claims description 3
- 239000007769 metal material Substances 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 64
- 239000002245 particle Substances 0.000 description 30
- 238000010438 heat treatment Methods 0.000 description 21
- 238000012864 cross contamination Methods 0.000 description 8
- 239000006249 magnetic particle Substances 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- 230000003321 amplification Effects 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 238000011112 process operation Methods 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 239000012139 lysis buffer Substances 0.000 description 5
- 238000007885 magnetic separation Methods 0.000 description 4
- 239000007858 starting material Substances 0.000 description 4
- 229910000831 Steel Inorganic materials 0.000 description 3
- 230000009089 cytolysis Effects 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000010959 steel Substances 0.000 description 3
- 239000011534 wash buffer Substances 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000012149 elution buffer Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08170320.9 | 2008-11-28 | ||
| EP08170320.9A EP2192186B1 (en) | 2008-11-28 | 2008-11-28 | System and method for the automated extraction of nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2686597A1 CA2686597A1 (en) | 2010-05-28 |
| CA2686597C true CA2686597C (en) | 2016-10-11 |
Family
ID=40433960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2686597A Active CA2686597C (en) | 2008-11-28 | 2009-11-27 | System and method for the automated extraction of nucleic acids |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20100137574A1 (enExample) |
| EP (1) | EP2192186B1 (enExample) |
| JP (1) | JP5268870B2 (enExample) |
| CN (1) | CN101747397B (enExample) |
| CA (1) | CA2686597C (enExample) |
| ES (1) | ES2567067T3 (enExample) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5846773B2 (ja) | 2010-06-29 | 2016-01-20 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | サンプルの分配 |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| EP2703820B1 (en) * | 2012-08-31 | 2019-08-28 | F. Hoffmann-La Roche AG | Mobile tip waste rack |
| TWI477755B (zh) * | 2013-06-04 | 2015-03-21 | Genereach Biotechnology Corp | 核酸萃取設備 |
| CN103789198B (zh) * | 2014-02-27 | 2015-09-16 | 苏州天隆生物科技有限公司 | 全自动核酸提取仪 |
| ES2854880T3 (es) * | 2014-06-11 | 2021-09-23 | Hoffmann La Roche | Procedimiento y sistema para el análisis de diagnóstico in vitro |
| CN105940305B (zh) * | 2014-06-17 | 2019-08-27 | 深圳迈瑞生物医疗电子股份有限公司 | 提取物分离装置及其工作方法 |
| KR102363674B1 (ko) * | 2015-04-06 | 2022-02-15 | 메소 스케일 테크놀러지즈, 엘엘시 | 소모성 진탕 장치를 포함하는, 전기화학발광을 이용하여 검정법을 수행하기 위한 고처리율 시스템 |
| CN106701527A (zh) * | 2015-08-11 | 2017-05-24 | 广州康昕瑞基因健康科技有限公司 | 一种试剂工作站 |
| CN105223054B (zh) * | 2015-10-13 | 2019-06-11 | 毛坤云 | 生物检材载体批量快速去除装置及去除方法 |
| JP6771903B2 (ja) * | 2016-02-29 | 2020-10-21 | シスメックス株式会社 | 検体前処理装置、検体前処理カートリッジおよび検体前処理方法 |
| CN109982778A (zh) * | 2016-09-23 | 2019-07-05 | 阿谢尔德克斯有限公司 | 流体系统及相关方法 |
| US20200363299A1 (en) * | 2016-11-23 | 2020-11-19 | Hangzhou Gene-Meta Medical Device Co., Ltd. | Apparatus for automating pretreatment of nucleic acid detection |
| CN106754349A (zh) * | 2017-01-13 | 2017-05-31 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | 加热振动装置及包括该加热振动装置的核酸提取设备 |
| CN107557356B (zh) * | 2017-09-05 | 2023-06-09 | 南京万承生物科技有限公司 | 一种小型多通道文库构建仪及构建文库的方法 |
| SG11202002507UA (en) * | 2018-03-02 | 2020-04-29 | Psomagen Inc | Method and system for high-throughput particle handling by use of magnetic fields and device |
| WO2019182903A1 (en) * | 2018-03-20 | 2019-09-26 | Nugen Technologies, Inc. | Methods for linear sample processing |
| CN108949553B (zh) * | 2018-08-23 | 2024-04-30 | 苏州英芮诚生化科技有限公司 | 一种核酸提取仪用弃废液装置 |
| US20210403846A1 (en) * | 2018-11-16 | 2021-12-30 | Aixinno Limited | Lab-ware and handling system to handle lab-ware in a cell culture process |
| CN110208063A (zh) * | 2019-06-27 | 2019-09-06 | 中国农业科学院农业质量标准与检测技术研究所 | 一种在线自动化磁性纳米材料震荡-解吸装置 |
| CN112226354A (zh) * | 2020-09-10 | 2021-01-15 | 襄阳市远杰精密机械有限公司 | 一种多样本通用型全自动核酸提取仪 |
| CN112920948A (zh) * | 2021-03-31 | 2021-06-08 | 苏州博捷特生物科技有限公司 | 一种核酸检测前处理仪 |
| EP4349953A4 (en) * | 2021-05-31 | 2025-06-04 | Universal Bio Research Co., Ltd. | SAMPLE TREATMENT DEVICE |
| CN113308364A (zh) * | 2021-06-09 | 2021-08-27 | 北京京东方技术开发有限公司 | 核酸提取基板、核酸提取设备和核酸提取方法 |
| CN113583812B (zh) * | 2021-08-04 | 2023-11-28 | 上海思路迪生物医学科技有限公司 | 一种适用于模块化工作站的核酸提取系统及方法 |
| CN118922523A (zh) * | 2022-09-30 | 2024-11-08 | 深圳华大生命科学研究院 | 生物芯片的自动化控制系统及其自动化控制方法 |
| CN117247834B (zh) * | 2023-11-17 | 2024-01-30 | 北京万泰生物药业股份有限公司 | 高通量全自动核酸检测仪器及方法 |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3115501B2 (ja) * | 1994-06-15 | 2000-12-11 | プレシジョン・システム・サイエンス株式会社 | 分注機を利用した磁性体の脱着制御方法及びこの方法によって処理される各種装置 |
| DE29623597U1 (de) * | 1996-11-08 | 1999-01-07 | Eppendorf - Netheler - Hinz Gmbh, 22339 Hamburg | Temperierblock mit Temperiereinrichtungen |
| US5779907A (en) * | 1996-12-06 | 1998-07-14 | Systems Research Laboratories, Inc. | Magnetic microplate separator |
| JP4015227B2 (ja) * | 1997-05-16 | 2007-11-28 | プレシジョン・システム・サイエンス株式会社 | 磁性体粒子を用いた化学反応処理装置及びその方法 |
| EP2316571A3 (en) * | 1998-05-01 | 2011-07-27 | Gen-Probe Incorporated | Automated diagnostic analyzer and method |
| EP1876451A3 (en) * | 1998-07-27 | 2012-02-29 | Hitachi, Ltd. | Handling method of body fluid sample and analysis apparatus using the same |
| JP3609945B2 (ja) * | 1998-08-28 | 2005-01-12 | 株式会社日立製作所 | 自動分析方法及び装置 |
| JP3391734B2 (ja) * | 1998-07-27 | 2003-03-31 | 株式会社日立製作所 | 生体サンプルの取扱い方法及び分析装置 |
| DE59912484D1 (de) * | 1998-07-31 | 2005-10-06 | Tecan Trading Ag Maennedorf | Magnetseparator |
| US6413780B1 (en) * | 1998-10-14 | 2002-07-02 | Abbott Laboratories | Structure and method for performing a determination of an item of interest in a sample |
| US5973138A (en) * | 1998-10-30 | 1999-10-26 | Becton Dickinson And Company | Method for purification and manipulation of nucleic acids using paramagnetic particles |
| DE19917375C2 (de) | 1999-04-16 | 2001-09-27 | Hamilton Bonaduz Ag Bonaduz | Pipettiereinheit |
| WO2001011364A1 (en) * | 1999-08-09 | 2001-02-15 | Precision System Science Co., Ltd. | Method for automatic labeling using dispenser, method for automatically separating target substance, method for determining base sequence, and automatic dispensing system |
| JP4045475B2 (ja) * | 1999-09-06 | 2008-02-13 | 東洋紡績株式会社 | 核酸・蛋白質精製装置 |
| US7250303B2 (en) * | 2001-07-20 | 2007-07-31 | Ortho-Clinical Diagnostics, Inc. | Chemistry system for a clinical analyzer |
| US20030059823A1 (en) * | 2001-09-21 | 2003-03-27 | Juki Corporation | Hybridization apparatus and method for detecting nucleic acid in sample using the same |
| JP3996416B2 (ja) * | 2001-09-21 | 2007-10-24 | Ｊｕｋｉ株式会社 | 核酸ハイブリダイゼーションにおけるｂ／ｆ分離方法 |
| JP2003093038A (ja) * | 2001-09-21 | 2003-04-02 | Juki Corp | ハイブリダイゼーション装置及びこれを用いたサンプル中の核酸検出方法 |
| JP4017369B2 (ja) * | 2001-09-28 | 2007-12-05 | 株式会社ヤクルト本社 | 核酸抽出装置 |
| US20080014610A1 (en) * | 2004-06-02 | 2008-01-17 | Arkray,Inc. | Container for Nucleic Acid Extraction, Method of Cleaning Solid Matrix and Relevant Cleaning Mechanism, and Method of Purifying Nucleic Acid |
| EP1615037A1 (en) * | 2004-07-06 | 2006-01-11 | Boehringer Mannheim Gmbh | An apparatus for liquid handling with multiple transfer tools |
| JP4630786B2 (ja) * | 2005-10-04 | 2011-02-09 | キヤノン株式会社 | 生化学処理装置、ｄｎａ増幅精製装置、該装置を含むｄｎａ検査装置 |
| JP4804278B2 (ja) * | 2006-08-30 | 2011-11-02 | ユニバーサル・バイオ・リサーチ株式会社 | マイクロプレート分割処理装置およびマイクロプレート分割処理方法 |
| JP5156905B2 (ja) * | 2007-04-20 | 2013-03-06 | 独立行政法人科学技術振興機構 | アプタマー生成装置、アプタマー生成方法 |
-
2008
- 2008-11-28 ES ES08170320.9T patent/ES2567067T3/es active Active
- 2008-11-28 EP EP08170320.9A patent/EP2192186B1/en active Active
-
2009
- 2009-11-23 US US12/623,774 patent/US20100137574A1/en not_active Abandoned
- 2009-11-27 JP JP2009270047A patent/JP5268870B2/ja active Active
- 2009-11-27 CA CA2686597A patent/CA2686597C/en active Active
- 2009-11-30 CN CN200910225856.8A patent/CN101747397B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2686597A1 (en) | 2010-05-28 |
| JP2010127941A (ja) | 2010-06-10 |
| CN101747397B (zh) | 2015-02-11 |
| ES2567067T3 (es) | 2016-04-19 |
| EP2192186A1 (en) | 2010-06-02 |
| CN101747397A (zh) | 2010-06-23 |
| JP5268870B2 (ja) | 2013-08-21 |
| US20100137574A1 (en) | 2010-06-03 |
| EP2192186B1 (en) | 2016-03-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20140725 |