CA2680794A1 - Trichoderma reesei .alpha.-amylase enhances saccharification of corn starch - Google Patents
Trichoderma reesei .alpha.-amylase enhances saccharification of corn starch Download PDFInfo
- Publication number
- CA2680794A1 CA2680794A1 CA002680794A CA2680794A CA2680794A1 CA 2680794 A1 CA2680794 A1 CA 2680794A1 CA 002680794 A CA002680794 A CA 002680794A CA 2680794 A CA2680794 A CA 2680794A CA 2680794 A1 CA2680794 A1 CA 2680794A1
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- Prior art keywords
- traa
- amylase
- variant
- glucose
- starch
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
- C12N9/242—Fungal source
Landscapes
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- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (5)
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| US90681207P | 2007-03-14 | 2007-03-14 | |
| US60/906,812 | 2007-03-14 | ||
| US60/906,811 | 2007-03-14 | ||
| PCT/US2008/056597 WO2008112727A2 (en) | 2007-03-14 | 2008-03-12 | Trichoderma reese a-amylase enhances saccharification of corn starch |
Publications (1)
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| CA2680794A1 true CA2680794A1 (en) | 2008-09-18 |
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| CA2680611A Expired - Fee Related CA2680611C (en) | 2007-03-14 | 2008-03-12 | Trichoderma reesei .alpha.-amylase is a maltogenic enzyme |
Family Applications After (1)
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| CA2680611A Expired - Fee Related CA2680611C (en) | 2007-03-14 | 2008-03-12 | Trichoderma reesei .alpha.-amylase is a maltogenic enzyme |
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| EP (2) | EP2132308B1 (enExample) |
| JP (2) | JP5194034B2 (enExample) |
| CN (2) | CN101657537B (enExample) |
| AT (2) | ATE533840T1 (enExample) |
| BR (2) | BRPI0808753A2 (enExample) |
| CA (2) | CA2680794A1 (enExample) |
| DK (2) | DK2132307T3 (enExample) |
| ES (2) | ES2376059T3 (enExample) |
| MX (2) | MX2009009692A (enExample) |
| WO (2) | WO2008112727A2 (enExample) |
Families Citing this family (24)
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| JP5192700B2 (ja) | 2007-01-19 | 2013-05-08 | サントリーホールディングス株式会社 | 新規配糖化酵素及びそれをコードするポリヌクレオチド |
| WO2008112727A2 (en) * | 2007-03-14 | 2008-09-18 | Danisco Us Inc., Genencor Division | Trichoderma reese a-amylase enhances saccharification of corn starch |
| MY157430A (en) | 2009-02-11 | 2016-06-15 | Xyleco Inc | Saccharifying biomass |
| GB0908770D0 (en) | 2009-04-24 | 2009-07-01 | Danisco | Method |
| GB201011513D0 (en) * | 2010-07-08 | 2010-08-25 | Danisco | Method |
| US20150111259A1 (en) | 2012-03-28 | 2015-04-23 | Danisco Us Inc. | Method for Making High Maltose Syrup |
| WO2013148663A1 (en) * | 2012-03-28 | 2013-10-03 | Danisco Us Inc. | Low temperature method for making high glucose syrup |
| US20140024064A1 (en) | 2012-07-23 | 2014-01-23 | Butamax(Tm) Advanced Biofuels Llc | Processes and systems for the production of fermentative alcohols |
| BR112015005439A8 (pt) | 2012-09-12 | 2022-01-11 | Butamax Advanced Biofuels Llc | Método para recuperar um produto alcoólico de um caldo de fermentação e sistema |
| EP2906706B1 (en) | 2012-10-11 | 2020-02-26 | Butamax Advanced Biofuels LLC | Processes and systems for the production of fermentation products |
| CN104822829A (zh) * | 2012-11-20 | 2015-08-05 | 丹尼斯科美国公司 | 具有产麦芽糖特性的淀粉酶 |
| US20180112203A1 (en) * | 2012-11-20 | 2018-04-26 | Danisco Us Inc. | Amylase with maltogenic properties |
| WO2014099415A1 (en) * | 2012-12-20 | 2014-06-26 | Danisco Us Inc. | Method of using alpha-amylase from aspergillus terreus and pullulanase for saccharification |
| WO2015099109A1 (ja) | 2013-12-27 | 2015-07-02 | 東レ株式会社 | 糖液の製造方法 |
| PL3166412T3 (pl) | 2014-07-08 | 2022-10-10 | Caravan Ingredients Inc. | Piekarnicze sposoby wytwarzania cukru i poprawy tekstury oraz utworzone z nich wyroby |
| JP6430753B2 (ja) * | 2014-09-09 | 2018-11-28 | 日清オイリオグループ株式会社 | 油脂組成物、該油脂組成物が配合された製菓製パン類 |
| AU2014406463B2 (en) | 2014-09-19 | 2020-04-02 | Xyleco, Inc. | Saccharides and saccharide compositions and mixtures |
| CN105524900A (zh) * | 2014-09-29 | 2016-04-27 | 湖南新鸿鹰生物工程有限公司 | 一种耐高温真菌α-淀粉酶 |
| CN105524839A (zh) * | 2014-09-29 | 2016-04-27 | 湖南新鸿鹰生物工程有限公司 | 一株高产耐高温真菌α-淀粉酶的里氏木霉 |
| CN105524899A (zh) * | 2014-09-29 | 2016-04-27 | 湖南新鸿鹰生物工程有限公司 | 一种变温调节生产耐高温真菌α-淀粉酶的方法 |
| CN104480690B (zh) * | 2014-12-01 | 2016-06-15 | 湖南新鸿鹰生物工程有限公司 | 一种含真菌α-淀粉酶的退浆复合酶及其制备方法 |
| EP3234166A1 (en) * | 2014-12-19 | 2017-10-25 | Danisco US Inc. | Glucoamylase blends |
| EP3576550A4 (en) | 2017-02-01 | 2020-09-09 | Manna Nutritional Group LLC | FLOUR HIGH IN FIBER, HIGH IN PROTEIN, LOW IN CARBOHYDRATES, SWEETNESS LIQUID, SWEETENERS, CEREALS AND THEIR PRODUCTION PROCESSES |
| FR3102489B1 (fr) * | 2019-10-24 | 2021-10-22 | Univ De Lorraine | Nouvelles souches de Carnobacterium maltaromaticumet leurs utilisations |
Family Cites Families (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5534046A (en) | 1978-09-01 | 1980-03-10 | Cpc International Inc | Novel glucoamyrase having excellent heat resistance and production |
| NO840200L (no) | 1983-01-28 | 1984-07-30 | Cefus Corp | Glukoamylase cdna. |
| US4536477A (en) | 1983-08-17 | 1985-08-20 | Cpc International Inc. | Thermostable glucoamylase and method for its production |
| US4587215A (en) | 1984-06-25 | 1986-05-06 | Uop Inc. | Highly thermostable amyloglucosidase |
| US4628031A (en) | 1984-09-18 | 1986-12-09 | Michigan Biotechnology Institute | Thermostable starch converting enzymes |
| DK154572C (da) | 1985-08-07 | 1989-04-24 | Novo Industri As | Enzymatisk detergentadditiv, detergent og fremgangsmaade til vask af tekstiler |
| DE3650202T3 (de) | 1985-08-29 | 2004-06-03 | Genencor International, Inc., South San Francisco | Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung. |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| GB8610600D0 (en) | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
| EP0287634B1 (en) | 1986-10-17 | 1995-01-11 | Novo Nordisk A/S | Positionally non-specific lipase from candida sp.; a method for producing it and its use |
| DK399387D0 (da) | 1987-07-31 | 1987-07-31 | Novo Industri As | Immobiliseret lipase og dennes anvendelse |
| ES2076939T3 (es) | 1987-08-28 | 1995-11-16 | Novo Nordisk As | Lipasa recombinante de humicola y procedimiento para la produccion de lipasas recombinantes de humicola. |
| DE4017150A1 (de) | 1990-05-28 | 1991-12-05 | Henkel Kgaa | Verfahren zur herstellung eines backaktiven pentosanase-praeparates |
| NL9001388A (nl) | 1990-06-19 | 1992-01-16 | Unilever Nv | Recombinant dna, cel die daarvan afgeleid dna bevat, enzym waarvoor het recombinant dna codeert en toepassingen daarvan. |
| US5162210A (en) | 1990-06-29 | 1992-11-10 | Iowa State University Research Foundation | Process for enzymatic hydrolysis of starch to glucose |
| PT98419B (pt) | 1990-07-24 | 1999-01-29 | Gist Brocades Nv | Processo para a preparacao de sequencias de adn que codificam para xilanases, de construcoes de adn que contem essas sequencias, paraa transformacao de hospedeiros microbianos com essas construcoes, para a preparacao de xilanases por expressao nesses hospedeiros e para a degradacao de xilano por accao dessas xilanases |
| US5290474A (en) | 1990-10-05 | 1994-03-01 | Genencor International, Inc. | Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp |
| US5475101A (en) | 1990-10-05 | 1995-12-12 | Genencor International, Inc. | DNA sequence encoding endoglucanase III cellulase |
| US5650322A (en) | 1990-10-05 | 1997-07-22 | Genencor International, Inc. | Methods for stonewashing fabrics using endoglucanases |
| JPH06502226A (ja) | 1990-10-05 | 1994-03-10 | ジェネンコア インターナショナル インコーポレーテッド | セルラーゼによる綿含有織物の処理方法 |
| US5246853A (en) | 1990-10-05 | 1993-09-21 | Genencor International, Inc. | Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I |
| EP1225227B1 (en) | 1990-12-10 | 2009-02-18 | Genencor International, Inc. | Improved saccharification of cellulose by cloning and amplification of the beta-glucosidase gene of trichoderma reesei |
| CA2106484A1 (en) | 1991-04-02 | 1992-10-03 | Martin Schulein | Xylanase, corresponding recombinant dna sequence, xylanase containing agent, and use of the agent |
| DK76893D0 (enExample) | 1993-06-28 | 1993-06-28 | Novo Nordisk As | |
| US5861271A (en) | 1993-12-17 | 1999-01-19 | Fowler; Timothy | Cellulase enzymes and systems for their expressions |
| EP0839224A1 (en) | 1995-07-19 | 1998-05-06 | Novo Nordisk A/S | Treatment of fabrics |
| KR100808499B1 (ko) | 1997-11-26 | 2008-02-29 | 노보자임스 에이/에스 | 열안정성 글루코아밀라제 |
| EP2316929B1 (en) | 1998-02-27 | 2016-04-27 | Novozymes A/S | Maltogenic alpha-amylase variants |
| CA2331340A1 (en) | 1998-07-15 | 2000-01-27 | Novozymes A/S | Glucoamylase variants |
| US6268328B1 (en) | 1998-12-18 | 2001-07-31 | Genencor International, Inc. | Variant EGIII-like cellulase compositions |
| MXPA05009566A (es) * | 2003-03-06 | 2005-12-14 | Diversa Corp | Amilasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas. |
| GB0305685D0 (en) * | 2003-03-12 | 2003-04-16 | Danisco | Enzyme |
| ATE542895T1 (de) | 2003-05-29 | 2012-02-15 | Danisco Us Inc | Neue trichoderma-gene |
| US7413887B2 (en) | 2004-05-27 | 2008-08-19 | Genecor International, Inc. | Trichoderma reesei glucoamylase and homologs thereof |
| DK1824970T3 (da) | 2004-11-30 | 2011-10-31 | Genencor Int | Thricoderma reesei-glycoamylase og homologer heraf |
| JP5184786B2 (ja) * | 2007-01-19 | 2013-04-17 | サントリーホールディングス株式会社 | フラボノイド類の配糖化方法 |
| WO2008112727A2 (en) * | 2007-03-14 | 2008-09-18 | Danisco Us Inc., Genencor Division | Trichoderma reese a-amylase enhances saccharification of corn starch |
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- 2008-03-12 ES ES08731952T patent/ES2376059T3/es active Active
- 2008-03-12 BR BRPI0808753-9A2A patent/BRPI0808753A2/pt not_active IP Right Cessation
- 2008-03-12 JP JP2009553732A patent/JP5194034B2/ja not_active Expired - Fee Related
- 2008-03-12 CN CN2008800081868A patent/CN101657537B/zh not_active Expired - Fee Related
- 2008-03-12 DK DK08731952.1T patent/DK2132307T3/da active
- 2008-03-12 MX MX2009009692A patent/MX2009009692A/es active IP Right Grant
- 2008-03-12 EP EP08743789A patent/EP2132308B1/en not_active Not-in-force
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- 2008-03-12 AT AT08731952T patent/ATE533840T1/de active
- 2008-03-12 EP EP08731952A patent/EP2132307B1/en not_active Not-in-force
- 2008-03-12 CA CA002680794A patent/CA2680794A1/en not_active Abandoned
- 2008-03-12 AT AT08743789T patent/ATE533841T1/de active
- 2008-03-12 CN CN2008800083365A patent/CN101636490B/zh not_active Expired - Fee Related
- 2008-03-12 US US12/532,083 patent/US8318157B2/en not_active Expired - Fee Related
- 2008-03-12 DK DK08743789.3T patent/DK2132308T3/da active
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- 2008-03-12 US US12/531,970 patent/US8916369B2/en not_active Expired - Fee Related
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- 2008-03-12 CA CA2680611A patent/CA2680611C/en not_active Expired - Fee Related
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2012
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Legal Events
| Date | Code | Title | Description |
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| EEER | Examination request |
Effective date: 20130306 |
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| FZDE | Discontinued |
Effective date: 20151112 |