CA2656327A1 - Procede de modification d'une macromolecule sans extraction prealable d'un echantillon - Google Patents
Procede de modification d'une macromolecule sans extraction prealable d'un echantillon Download PDFInfo
- Publication number
- CA2656327A1 CA2656327A1 CA002656327A CA2656327A CA2656327A1 CA 2656327 A1 CA2656327 A1 CA 2656327A1 CA 002656327 A CA002656327 A CA 002656327A CA 2656327 A CA2656327 A CA 2656327A CA 2656327 A1 CA2656327 A1 CA 2656327A1
- Authority
- CA
- Canada
- Prior art keywords
- dna
- sample
- macromolecule
- bisulfite
- modification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 101
- 229920002521 macromolecule Polymers 0.000 title claims abstract description 26
- 238000000605 extraction Methods 0.000 title claims abstract description 14
- 239000000126 substance Substances 0.000 claims abstract description 10
- 239000000543 intermediate Substances 0.000 claims abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 45
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims description 39
- 230000004048 modification Effects 0.000 claims description 37
- 238000012986 modification Methods 0.000 claims description 37
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 36
- 210000001519 tissue Anatomy 0.000 claims description 32
- 210000002700 urine Anatomy 0.000 claims description 29
- 239000003153 chemical reaction reagent Substances 0.000 claims description 26
- 238000000746 purification Methods 0.000 claims description 23
- 210000004027 cell Anatomy 0.000 claims description 21
- 238000005869 desulfonation reaction Methods 0.000 claims description 21
- 230000006326 desulfonation Effects 0.000 claims description 17
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 13
- 238000011534 incubation Methods 0.000 claims description 13
- 239000006166 lysate Substances 0.000 claims description 12
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 10
- 150000002632 lipids Chemical class 0.000 claims description 10
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 235000014633 carbohydrates Nutrition 0.000 claims description 9
- 230000011987 methylation Effects 0.000 claims description 9
- 238000007069 methylation reaction Methods 0.000 claims description 9
- 238000001574 biopsy Methods 0.000 claims description 8
- 230000015572 biosynthetic process Effects 0.000 claims description 8
- 150000001720 carbohydrates Chemical class 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 210000001124 body fluid Anatomy 0.000 claims description 7
- 239000010839 body fluid Substances 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical group O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 6
- 238000005576 amination reaction Methods 0.000 claims description 6
- 239000012188 paraffin wax Substances 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 239000002207 metabolite Substances 0.000 claims description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000005909 Kieselgur Substances 0.000 claims description 4
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical group [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims description 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 4
- 230000001413 cellular effect Effects 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 4
- 238000006481 deamination reaction Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 239000002502 liposome Substances 0.000 claims description 4
- 210000000056 organ Anatomy 0.000 claims description 4
- 238000007254 oxidation reaction Methods 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 239000000377 silicon dioxide Substances 0.000 claims description 4
- 238000004513 sizing Methods 0.000 claims description 4
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 claims description 4
- 230000006287 biotinylation Effects 0.000 claims description 3
- 238000007413 biotinylation Methods 0.000 claims description 3
- 210000004369 blood Anatomy 0.000 claims description 3
- 239000008280 blood Substances 0.000 claims description 3
- 230000002438 mitochondrial effect Effects 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims description 2
- 102000035195 Peptidases Human genes 0.000 claims description 2
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- 230000000747 cardiac effect Effects 0.000 claims description 2
- 210000000170 cell membrane Anatomy 0.000 claims description 2
- 239000000356 contaminant Substances 0.000 claims description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical group NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 2
- 230000009615 deamination Effects 0.000 claims description 2
- 230000022811 deglycosylation Effects 0.000 claims description 2
- 230000030609 dephosphorylation Effects 0.000 claims description 2
- 238000006209 dephosphorylation reaction Methods 0.000 claims description 2
- 239000003599 detergent Substances 0.000 claims description 2
- WBZKQQHYRPRKNJ-UHFFFAOYSA-L disulfite Chemical compound [O-]S(=O)S([O-])(=O)=O WBZKQQHYRPRKNJ-UHFFFAOYSA-L 0.000 claims description 2
- 210000003038 endothelium Anatomy 0.000 claims description 2
- 230000002255 enzymatic effect Effects 0.000 claims description 2
- 210000000981 epithelium Anatomy 0.000 claims description 2
- 238000003682 fluorination reaction Methods 0.000 claims description 2
- 230000002496 gastric effect Effects 0.000 claims description 2
- 230000013595 glycosylation Effects 0.000 claims description 2
- 238000006206 glycosylation reaction Methods 0.000 claims description 2
- 210000004020 intracellular membrane Anatomy 0.000 claims description 2
- 108020004999 messenger RNA Proteins 0.000 claims description 2
- 108091070501 miRNA Proteins 0.000 claims description 2
- 239000000693 micelle Substances 0.000 claims description 2
- 239000002679 microRNA Substances 0.000 claims description 2
- 210000003205 muscle Anatomy 0.000 claims description 2
- 230000001537 neural effect Effects 0.000 claims description 2
- 108020004707 nucleic acids Proteins 0.000 claims description 2
- 102000039446 nucleic acids Human genes 0.000 claims description 2
- 150000007523 nucleic acids Chemical class 0.000 claims description 2
- 230000003647 oxidation Effects 0.000 claims description 2
- 230000026731 phosphorylation Effects 0.000 claims description 2
- 238000006366 phosphorylation reaction Methods 0.000 claims description 2
- 210000002381 plasma Anatomy 0.000 claims description 2
- 210000002706 plastid Anatomy 0.000 claims description 2
- 229920000642 polymer Polymers 0.000 claims description 2
- 235000019833 protease Nutrition 0.000 claims description 2
- 210000003296 saliva Anatomy 0.000 claims description 2
- 210000002966 serum Anatomy 0.000 claims description 2
- 229940001584 sodium metabisulfite Drugs 0.000 claims description 2
- 235000010262 sodium metabisulphite Nutrition 0.000 claims description 2
- 238000000527 sonication Methods 0.000 claims description 2
- 229940035893 uracil Drugs 0.000 claims description 2
- 230000003612 virological effect Effects 0.000 claims description 2
- 125000000837 carbohydrate group Chemical group 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 claims 1
- 125000003473 lipid group Chemical group 0.000 claims 1
- 230000002934 lysing effect Effects 0.000 claims 1
- 230000002503 metabolic effect Effects 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 79
- 239000000523 sample Substances 0.000 description 61
- 239000008188 pellet Substances 0.000 description 25
- 238000006243 chemical reaction Methods 0.000 description 23
- 102100030943 Glutathione S-transferase P Human genes 0.000 description 19
- 101001010139 Homo sapiens Glutathione S-transferase P Proteins 0.000 description 19
- 239000011534 wash buffer Substances 0.000 description 18
- 239000003550 marker Substances 0.000 description 14
- 238000003556 assay Methods 0.000 description 13
- 230000008836 DNA modification Effects 0.000 description 12
- 239000000203 mixture Substances 0.000 description 11
- 230000007067 DNA methylation Effects 0.000 description 10
- 239000000872 buffer Substances 0.000 description 10
- 239000002699 waste material Substances 0.000 description 10
- 238000004113 cell culture Methods 0.000 description 9
- 238000007855 methylation-specific PCR Methods 0.000 description 9
- 239000013024 dilution buffer Substances 0.000 description 8
- 210000002307 prostate Anatomy 0.000 description 8
- 238000007399 DNA isolation Methods 0.000 description 7
- 239000011536 extraction buffer Substances 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- 108010067770 Endopeptidase K Proteins 0.000 description 6
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 239000008096 xylene Substances 0.000 description 6
- 238000007400 DNA extraction Methods 0.000 description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 239000012148 binding buffer Substances 0.000 description 5
- 230000029087 digestion Effects 0.000 description 5
- 239000011159 matrix material Substances 0.000 description 5
- 102000007469 Actins Human genes 0.000 description 4
- 108010085238 Actins Proteins 0.000 description 4
- 239000007983 Tris buffer Substances 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000009987 spinning Methods 0.000 description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical class CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- 239000013592 cell lysate Substances 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 239000012139 lysis buffer Substances 0.000 description 3
- 238000007427 paired t-test Methods 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- -1 R-Actin Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- 238000000540 analysis of variance Methods 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000011033 desalting Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000012149 elution buffer Substances 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000001543 one-way ANOVA Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 210000005267 prostate cell Anatomy 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 101100491335 Caenorhabditis elegans mat-2 gene Proteins 0.000 description 1
- 108091029523 CpG island Proteins 0.000 description 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 230000007018 DNA scission Effects 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 101000993380 Homo sapiens Hypermethylated in cancer 1 protein Proteins 0.000 description 1
- 102100031612 Hypermethylated in cancer 1 protein Human genes 0.000 description 1
- 108700011259 MicroRNAs Proteins 0.000 description 1
- YGJHZCLPZAZIHH-UHFFFAOYSA-N N-Nitrosodi-n-butylamine Chemical compound CCCCN(N=O)CCCC YGJHZCLPZAZIHH-UHFFFAOYSA-N 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
- 241000011102 Thera Species 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 108020000999 Viral RNA Proteins 0.000 description 1
- 230000001594 aberrant effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 208000012191 childhood neoplasm Diseases 0.000 description 1
- 238000005094 computer simulation Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 238000002349 difference gel electrophoresis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007831 electrophysiology Effects 0.000 description 1
- 238000002001 electrophysiology Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000006607 hypermethylation Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000010627 oxidative phosphorylation Effects 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000002976 reverse transcriptase assay Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 229960001866 silicon dioxide Drugs 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 125000003396 thiol group Chemical class [H]S* 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Urology & Nephrology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US81787606P | 2006-06-29 | 2006-06-29 | |
| US60/817,876 | 2006-06-29 | ||
| US82879006P | 2006-10-10 | 2006-10-10 | |
| US60/828,790 | 2006-10-10 | ||
| PCT/US2007/015329 WO2008002680A2 (fr) | 2006-06-29 | 2007-06-29 | Procédé de modification d'une macromolécule sans extraction préalable d'un échantillon |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2656327A1 true CA2656327A1 (fr) | 2008-01-03 |
Family
ID=38846340
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002656327A Abandoned CA2656327A1 (fr) | 2006-06-29 | 2007-06-29 | Procede de modification d'une macromolecule sans extraction prealable d'un echantillon |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP2041316A4 (fr) |
| JP (1) | JP2009542216A (fr) |
| KR (1) | KR20090036121A (fr) |
| BR (1) | BRPI0713927A2 (fr) |
| CA (1) | CA2656327A1 (fr) |
| IL (1) | IL196198A0 (fr) |
| MX (1) | MX2009000122A (fr) |
| WO (1) | WO2008002680A2 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8298852B2 (en) | 2008-12-29 | 2012-10-30 | Jusung Engineering Co., Ltd. | Thin film type solar cell and method for manufacturing the same |
| EP2407540A1 (fr) * | 2010-07-15 | 2012-01-18 | Qiagen GmbH | Procédé de purification d'un acide nucléique cible |
| US20150368636A1 (en) * | 2013-01-30 | 2015-12-24 | Exact Sciences Corporation | Treatment of a sample vessel |
| CN107821989B (zh) * | 2017-12-04 | 2020-11-17 | 南京农业大学 | 一种提高类pse鸡胸肉肌原纤维蛋白凝胶品质的糖基化方法 |
| WO2022066844A1 (fr) * | 2020-09-24 | 2022-03-31 | Case Western Reserve University | Compositions et procédés de préservation de la méthylation de l'adn |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10304219B3 (de) * | 2003-01-30 | 2004-08-19 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität |
-
2007
- 2007-06-29 KR KR1020097001936A patent/KR20090036121A/ko not_active Application Discontinuation
- 2007-06-29 CA CA002656327A patent/CA2656327A1/fr not_active Abandoned
- 2007-06-29 MX MX2009000122A patent/MX2009000122A/es unknown
- 2007-06-29 BR BRPI0713927-6A patent/BRPI0713927A2/pt not_active Application Discontinuation
- 2007-06-29 JP JP2009518328A patent/JP2009542216A/ja active Pending
- 2007-06-29 WO PCT/US2007/015329 patent/WO2008002680A2/fr active Application Filing
- 2007-06-29 EP EP07810136A patent/EP2041316A4/fr not_active Withdrawn
-
2008
- 2008-12-25 IL IL196198A patent/IL196198A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP2041316A2 (fr) | 2009-04-01 |
| IL196198A0 (en) | 2009-09-22 |
| WO2008002680A2 (fr) | 2008-01-03 |
| MX2009000122A (es) | 2009-01-26 |
| EP2041316A4 (fr) | 2009-12-09 |
| JP2009542216A (ja) | 2009-12-03 |
| WO2008002680A3 (fr) | 2008-11-27 |
| KR20090036121A (ko) | 2009-04-13 |
| BRPI0713927A2 (pt) | 2013-01-08 |
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