WO2008002680A3 - Procédé de modification d'une macromolécule sans extraction préalable d'un échantillon - Google Patents

Procédé de modification d'une macromolécule sans extraction préalable d'un échantillon Download PDF

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Publication number
WO2008002680A3
WO2008002680A3 PCT/US2007/015329 US2007015329W WO2008002680A3 WO 2008002680 A3 WO2008002680 A3 WO 2008002680A3 US 2007015329 W US2007015329 W US 2007015329W WO 2008002680 A3 WO2008002680 A3 WO 2008002680A3
Authority
WO
WIPO (PCT)
Prior art keywords
macromolecule
sample
modifying
prior extraction
converting
Prior art date
Application number
PCT/US2007/015329
Other languages
English (en)
Other versions
WO2008002680A2 (fr
Inventor
Christopher Steele
Abhijit Mazumder
Darin Oppenheimer
Sean Wuxiong Cao
Carrie Trust
George Green
Jyoti Mehrotra
Tatiana Vener
Shobha Varde
Original Assignee
Veridex Llc
Christopher Steele
Abhijit Mazumder
Darin Oppenheimer
Sean Wuxiong Cao
Carrie Trust
George Green
Jyoti Mehrotra
Tatiana Vener
Shobha Varde
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Veridex Llc, Christopher Steele, Abhijit Mazumder, Darin Oppenheimer, Sean Wuxiong Cao, Carrie Trust, George Green, Jyoti Mehrotra, Tatiana Vener, Shobha Varde filed Critical Veridex Llc
Priority to CA002656327A priority Critical patent/CA2656327A1/fr
Priority to MX2009000122A priority patent/MX2009000122A/es
Priority to BRPI0713927-6A priority patent/BRPI0713927A2/pt
Priority to JP2009518328A priority patent/JP2009542216A/ja
Priority to EP07810136A priority patent/EP2041316A4/fr
Publication of WO2008002680A2 publication Critical patent/WO2008002680A2/fr
Publication of WO2008002680A3 publication Critical patent/WO2008002680A3/fr
Priority to IL196198A priority patent/IL196198A0/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un procédé de modification d'une macromolécule sans extraction préalable d'un échantillon par la conversion de la macromolécule dans l'échantillon avec un produit chimique, l'élimination ou la conversion d'intermédiaires chimiques, le cas échéant, et la purification de la macromolécule modifiée ainsi obtenue.
PCT/US2007/015329 2006-06-29 2007-06-29 Procédé de modification d'une macromolécule sans extraction préalable d'un échantillon WO2008002680A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA002656327A CA2656327A1 (fr) 2006-06-29 2007-06-29 Procede de modification d'une macromolecule sans extraction prealable d'un echantillon
MX2009000122A MX2009000122A (es) 2006-06-29 2007-06-29 Un metodo para la modificacion de una macromolecula sin extraccion previa de una muestra.
BRPI0713927-6A BRPI0713927A2 (pt) 2006-06-29 2007-06-29 mÉtodo de modificar uma macromolÉcula sem extraÇço anterior de uma amostra
JP2009518328A JP2009542216A (ja) 2006-06-29 2007-06-29 サンプルから事前に抽出せずに高分子を修飾する方法
EP07810136A EP2041316A4 (fr) 2006-06-29 2007-06-29 Procédé de modification d'une macromolécule sans extraction préalable d'un échantillon
IL196198A IL196198A0 (en) 2006-06-29 2008-12-25 A method of modifying a macromolecule without prior extraction from a sample

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US81787606P 2006-06-29 2006-06-29
US60/817,876 2006-06-29
US82879006P 2006-10-10 2006-10-10
US60/828,790 2006-10-10

Publications (2)

Publication Number Publication Date
WO2008002680A2 WO2008002680A2 (fr) 2008-01-03
WO2008002680A3 true WO2008002680A3 (fr) 2008-11-27

Family

ID=38846340

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/015329 WO2008002680A2 (fr) 2006-06-29 2007-06-29 Procédé de modification d'une macromolécule sans extraction préalable d'un échantillon

Country Status (8)

Country Link
EP (1) EP2041316A4 (fr)
JP (1) JP2009542216A (fr)
KR (1) KR20090036121A (fr)
BR (1) BRPI0713927A2 (fr)
CA (1) CA2656327A1 (fr)
IL (1) IL196198A0 (fr)
MX (1) MX2009000122A (fr)
WO (1) WO2008002680A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8298852B2 (en) 2008-12-29 2012-10-30 Jusung Engineering Co., Ltd. Thin film type solar cell and method for manufacturing the same
EP2407540A1 (fr) * 2010-07-15 2012-01-18 Qiagen GmbH Procédé de purification d'un acide nucléique cible
WO2014120918A1 (fr) * 2013-01-30 2014-08-07 Exact Sciences Corporation Traitement de vaisseau échantillonnés
CN107821989B (zh) * 2017-12-04 2020-11-17 南京农业大学 一种提高类pse鸡胸肉肌原纤维蛋白凝胶品质的糖基化方法
WO2022066844A1 (fr) * 2020-09-24 2022-03-31 Case Western Reserve University Compositions et procédés de préservation de la méthylation de l'adn

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10304219B3 (de) * 2003-01-30 2004-08-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ALLERSON C.R., J. AM. CHEM. SOC., vol. 119, 1997, pages 7423 - 7433, XP008101741 *
KORNBLAU ET AL., LEUK. LYMPH., vol. 35, no. 3-4, 1999, pages 283 - 288, XP008101776 *
MARU N. ET AL., NUCLEIC ACIDS SYMPOSIUM SERIES, 2004, pages 95 - 96, XP008101743 *
MERI S. ET AL.: "Proteomics: posttranslational modifications, immune responses and current analytical tools", BIOMOLECULAR ENGINEERING, vol. 18, no. 5, November 2001 (2001-11-01), pages 213 - 220, XP004308886 *
SUGDEN M.C. ET AL., AM. J. PHYSIOL. ENDOCRINOL. METABOL., vol. 284, 2003, pages E855 - E862, XP008101778 *

Also Published As

Publication number Publication date
IL196198A0 (en) 2009-09-22
EP2041316A4 (fr) 2009-12-09
KR20090036121A (ko) 2009-04-13
WO2008002680A2 (fr) 2008-01-03
CA2656327A1 (fr) 2008-01-03
BRPI0713927A2 (pt) 2013-01-08
EP2041316A2 (fr) 2009-04-01
MX2009000122A (es) 2009-01-26
JP2009542216A (ja) 2009-12-03

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