EP2041316A4 - Procédé de modification d'une macromolécule sans extraction préalable d'un échantillon - Google Patents

Procédé de modification d'une macromolécule sans extraction préalable d'un échantillon

Info

Publication number
EP2041316A4
EP2041316A4 EP07810136A EP07810136A EP2041316A4 EP 2041316 A4 EP2041316 A4 EP 2041316A4 EP 07810136 A EP07810136 A EP 07810136A EP 07810136 A EP07810136 A EP 07810136A EP 2041316 A4 EP2041316 A4 EP 2041316A4
Authority
EP
European Patent Office
Prior art keywords
macromolecule
modifying
sample
prior extraction
extraction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP07810136A
Other languages
German (de)
English (en)
Other versions
EP2041316A2 (fr
Inventor
Christopher Steele
Abhijit Mazumder
Darin Oppenheimer
Sean Wuxiong Cao
Carrie Trust
George Green
Jyoti Mehrotra
Tatiana Vener
Shobha Varde
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Janssen Diagnostics LLC
Original Assignee
Janssen Diagnostics LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Janssen Diagnostics LLC filed Critical Janssen Diagnostics LLC
Publication of EP2041316A2 publication Critical patent/EP2041316A2/fr
Publication of EP2041316A4 publication Critical patent/EP2041316A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
EP07810136A 2006-06-29 2007-06-29 Procédé de modification d'une macromolécule sans extraction préalable d'un échantillon Withdrawn EP2041316A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US81787606P 2006-06-29 2006-06-29
US82879006P 2006-10-10 2006-10-10
PCT/US2007/015329 WO2008002680A2 (fr) 2006-06-29 2007-06-29 Procédé de modification d'une macromolécule sans extraction préalable d'un échantillon

Publications (2)

Publication Number Publication Date
EP2041316A2 EP2041316A2 (fr) 2009-04-01
EP2041316A4 true EP2041316A4 (fr) 2009-12-09

Family

ID=38846340

Family Applications (1)

Application Number Title Priority Date Filing Date
EP07810136A Withdrawn EP2041316A4 (fr) 2006-06-29 2007-06-29 Procédé de modification d'une macromolécule sans extraction préalable d'un échantillon

Country Status (8)

Country Link
EP (1) EP2041316A4 (fr)
JP (1) JP2009542216A (fr)
KR (1) KR20090036121A (fr)
BR (1) BRPI0713927A2 (fr)
CA (1) CA2656327A1 (fr)
IL (1) IL196198A0 (fr)
MX (1) MX2009000122A (fr)
WO (1) WO2008002680A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8298852B2 (en) 2008-12-29 2012-10-30 Jusung Engineering Co., Ltd. Thin film type solar cell and method for manufacturing the same
EP2407540A1 (fr) * 2010-07-15 2012-01-18 Qiagen GmbH Procédé de purification d'un acide nucléique cible
WO2014120918A1 (fr) * 2013-01-30 2014-08-07 Exact Sciences Corporation Traitement de vaisseau échantillonnés
CN107821989B (zh) * 2017-12-04 2020-11-17 南京农业大学 一种提高类pse鸡胸肉肌原纤维蛋白凝胶品质的糖基化方法
WO2022066844A1 (fr) * 2020-09-24 2022-03-31 Case Western Reserve University Compositions et procédés de préservation de la méthylation de l'adn

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004067775A2 (fr) * 2003-01-30 2004-08-12 Epigenomics Ag Technique de detection de configurations de methylation de cytosine a haute sensibilite

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004067775A2 (fr) * 2003-01-30 2004-08-12 Epigenomics Ag Technique de detection de configurations de methylation de cytosine a haute sensibilite

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GRUNAU C; CLARK S J; ROSENTHAL A: "Bisulfite genomic sequencing: systematic investigation of critical experimental parameters", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 29, no. 13, 1 January 2001 (2001-01-01), pages E65 - 1, XP002241346, ISSN: 0305-1048 *
OLEK A; ET AL: "A modified an improved method for bisulphite based cytosine methylation analysis", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 24, no. 24, 1 January 1996 (1996-01-01), pages 5064 - 5066, XP002106408, ISSN: 0305-1048 *
SHIRAISHI MASAHIKO; HAYATSU HIKOYA: "High-speed conversion of cytosine to uracil in bisulfite genomic sequencing analysis of DNA methylation.", DNA RESEARCH : AN INTERNATIONAL JOURNAL FOR RAPID PUBLICATION OF REPORTS ON GENES AND GENOMES 31 DEC 2004, vol. 11, no. 6, 31 December 2004 (2004-12-31), pages 409 - 415, XP002545192, ISSN: 1340-2838 *

Also Published As

Publication number Publication date
KR20090036121A (ko) 2009-04-13
WO2008002680A2 (fr) 2008-01-03
EP2041316A2 (fr) 2009-04-01
BRPI0713927A2 (pt) 2013-01-08
CA2656327A1 (fr) 2008-01-03
IL196198A0 (en) 2009-09-22
WO2008002680A3 (fr) 2008-11-27
MX2009000122A (es) 2009-01-26
JP2009542216A (ja) 2009-12-03

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RIN1 Information on inventor provided before grant (corrected)

Inventor name: VARDE, SHOBHA

Inventor name: VENER, TATIANA

Inventor name: MEHROTRA, JYOTI

Inventor name: GREEN, GEORGE

Inventor name: TRUST, CARRIE

Inventor name: CAO, SEAN, WUXIONG

Inventor name: OPPENHEIMER, DARIN

Inventor name: MAZUMDER, ABHIJIT

Inventor name: STEELE, CHRISTOPHER

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