CA2635968A1 - Cryoprotective compositions and methods of using same - Google Patents
Cryoprotective compositions and methods of using same Download PDFInfo
- Publication number
- CA2635968A1 CA2635968A1 CA002635968A CA2635968A CA2635968A1 CA 2635968 A1 CA2635968 A1 CA 2635968A1 CA 002635968 A CA002635968 A CA 002635968A CA 2635968 A CA2635968 A CA 2635968A CA 2635968 A1 CA2635968 A1 CA 2635968A1
- Authority
- CA
- Canada
- Prior art keywords
- cryoprotective
- composition
- nanostructures
- cellular matter
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 230000000959 cryoprotective effect Effects 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims description 107
- 239000002086 nanomaterial Substances 0.000 claims abstract description 110
- 239000007788 liquid Substances 0.000 claims abstract description 74
- 239000002577 cryoprotective agent Substances 0.000 claims abstract description 35
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- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 69
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Classifications
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/125—Specific component of sample, medium or buffer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/113—Real time assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/155—Particles of a defined size, e.g. nanoparticles
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Public Health (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Immunology (AREA)
- Veterinary Medicine (AREA)
- Physics & Mathematics (AREA)
- Oncology (AREA)
- Nanotechnology (AREA)
- Communicable Diseases (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medical Informatics (AREA)
- Epidemiology (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cosmetics (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US75585106P | 2006-01-04 | 2006-01-04 | |
| US75585006P | 2006-01-04 | 2006-01-04 | |
| US75585206P | 2006-01-04 | 2006-01-04 | |
| US60/755,851 | 2006-01-04 | ||
| US60/755,852 | 2006-01-04 | ||
| US11/324,586 US20060177852A1 (en) | 2001-12-12 | 2006-01-04 | Solid-fluid composition |
| US11/324,586 | 2006-01-04 | ||
| US60/755,850 | 2006-01-04 | ||
| PCT/IL2007/000013 WO2007077560A2 (en) | 2006-01-04 | 2007-01-04 | Cryoprotective compositions and methods of using same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2635968A1 true CA2635968A1 (en) | 2007-07-12 |
Family
ID=39735923
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002635975A Abandoned CA2635975A1 (en) | 2006-01-04 | 2007-01-04 | Compositions and methods for enhancing in-vivo uptake of pharmaceutical agents |
| CA002635978A Abandoned CA2635978A1 (en) | 2006-01-04 | 2007-01-04 | Solid-fluid composition |
| CA002635976A Abandoned CA2635976A1 (en) | 2006-01-04 | 2007-01-04 | Antiseptic compositions and methods of using same |
| CA002635968A Abandoned CA2635968A1 (en) | 2006-01-04 | 2007-01-04 | Cryoprotective compositions and methods of using same |
Family Applications Before (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002635975A Abandoned CA2635975A1 (en) | 2006-01-04 | 2007-01-04 | Compositions and methods for enhancing in-vivo uptake of pharmaceutical agents |
| CA002635978A Abandoned CA2635978A1 (en) | 2006-01-04 | 2007-01-04 | Solid-fluid composition |
| CA002635976A Abandoned CA2635976A1 (en) | 2006-01-04 | 2007-01-04 | Antiseptic compositions and methods of using same |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20090029340A1 (OSRAM) |
| EP (4) | EP1981988A2 (OSRAM) |
| JP (4) | JP2009524600A (OSRAM) |
| KR (4) | KR20080090489A (OSRAM) |
| AU (4) | AU2007203958A1 (OSRAM) |
| CA (4) | CA2635975A1 (OSRAM) |
| WO (4) | WO2007077560A2 (OSRAM) |
Families Citing this family (58)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060177852A1 (en) * | 2001-12-12 | 2006-08-10 | Do-Coop Technologies Ltd. | Solid-fluid composition |
| US20090004296A1 (en) * | 2006-01-04 | 2009-01-01 | Do-Coop Technologies Ltd. | Antiseptic Compositions and Methods of Using Same |
| US20090253613A1 (en) * | 2006-01-04 | 2009-10-08 | Do-Coop Technologies Ltd. | Solid-Fluid Composition |
| WO2008081456A2 (en) * | 2007-01-04 | 2008-07-10 | Do-Coop Technologies Ltd. | Composition and method for enhancing cell growth and cell fusion |
| KR20090095674A (ko) * | 2007-01-04 | 2009-09-09 | 두-쿱 테크놀로지스 리미티드 | 분석물의 검출 |
| GB0705626D0 (en) * | 2007-03-23 | 2007-05-02 | Royal Veterinary College | Method for enhancing sperm survival |
| EP2187928A2 (en) * | 2007-09-11 | 2010-05-26 | Mondobiotech Laboratories AG | Use of anti-inflammatory peptide 1 as a therapeutic agent |
| WO2009033763A1 (en) * | 2007-09-11 | 2009-03-19 | Mondobiotech Laboratories Ag | Nafarelin and carboxymethyl-phe-leu as therapeutic agents |
| US20090081785A1 (en) | 2007-09-24 | 2009-03-26 | Hememics Biotechnologies, Inc. | Desiccated Biologics And Methods Of Preparing The Same |
| KR101467014B1 (ko) * | 2007-11-09 | 2014-12-01 | 프랙스에어 테크놀로지, 인코포레이티드 | 생물학적 물질의 프로그램 냉동을 위한 방법 및 시스템 |
| US20100285141A1 (en) * | 2008-01-03 | 2010-11-11 | Do-Coop Technologies Ltd. | Compositions and methods for enhancing the activity of podophyllotoxin |
| AR071478A1 (es) | 2008-04-17 | 2010-06-23 | Baxter Healthcare Sa | Peptidos de bajo peso molecular con actividad procoagulante para el tratamiento de pacientes con deficiencia de factor v (fv), fvii, fviii, fx y/o fxi |
| US20100209540A1 (en) * | 2008-11-05 | 2010-08-19 | Pharmacaribe | Inhalation formulation for treating and prophylactic use in bacteria, mycobacterial and fungal respiratory infections |
| CN102387793B (zh) | 2009-02-11 | 2014-10-01 | 雷蒙特亚特特拉维夫大学有限公司 | 包括银离子源和薄荷醇的抗菌组合物及其用途 |
| US9700038B2 (en) | 2009-02-25 | 2017-07-11 | Genea Limited | Cryopreservation of biological cells and tissues |
| KR101092411B1 (ko) * | 2009-07-22 | 2011-12-09 | (주)시지바이오 | 이식용 동종 피부의 처리 방법 및 그로부터 제조된 동결보존동종피부 |
| PL2301943T3 (pl) * | 2009-09-08 | 2014-06-30 | Heraeus Precious Metals Gmbh | Krystalizacja epidaunorubicyny x HCI |
| WO2011049200A1 (ja) | 2009-10-23 | 2011-04-28 | 学校法人産業医科大学 | 掻痒抑制剤 |
| US9943075B2 (en) * | 2010-02-17 | 2018-04-17 | Hememics Biotechnologies, Inc. | Preservation solutions for biologics and methods related thereto |
| US8529883B2 (en) * | 2010-05-07 | 2013-09-10 | Fibrocell Technologies, Inc. | Dosage unit formulations of autologous dermal fibroblasts |
| HUE062745T2 (hu) | 2010-05-28 | 2023-12-28 | Genea Ip Holdings Pty Ltd | Továbbfejlesztett mikromanipulációs és tároló készülék és eljárások |
| US20120128845A1 (en) * | 2010-11-22 | 2012-05-24 | Carol Ann Tosaya | Ice, toxicity, thermal-stress and cold-fracture management during cryopreserving encapsulation of specimens using controlled ice nucleation |
| CN102206285B (zh) * | 2011-04-19 | 2013-02-13 | 兰州大学 | 基于内吗啡肽2和神经肽ff的嵌合肽及其合成和应用 |
| PT2717898T (pt) | 2011-06-10 | 2019-05-20 | Bioverativ Therapeutics Inc | Compostos pró-coagulantes e processos para a sua utilização |
| US20140227688A1 (en) | 2011-09-26 | 2014-08-14 | Qiagen Gmbh | Stabilisation and isolation of extracellular nucleic acids |
| US10676780B2 (en) | 2011-09-26 | 2020-06-09 | Qiagen Gmbh | Stabilisation and isolation of extracellular nucleic acids |
| US11021733B2 (en) | 2011-09-26 | 2021-06-01 | Qiagen Gmbh | Stabilization and isolation of extracellular nucleic acids |
| US20140308364A1 (en) * | 2011-11-16 | 2014-10-16 | The University Of North Carolina At Chapel Hill | Gelatinous hydroxyapatite-nanocomposites |
| WO2013096659A1 (en) * | 2011-12-20 | 2013-06-27 | Cook General Biotechnology Llc | Methods and compositions for storage of animal cells |
| EP2825635B1 (en) * | 2012-03-14 | 2020-04-15 | Membrane Protective Technologies, Inc. | System and substances for cryopreservation of viable cells |
| EP3693000B1 (en) | 2012-06-08 | 2022-03-02 | Bioverativ Therapeutics Inc. | Procoagulant compounds |
| KR101410589B1 (ko) * | 2012-07-19 | 2014-06-20 | 주식회사 바이오에프디엔씨 | 뉴로펩타이드 유도체 및 이를 함유하는 피부 외용제 조성물 |
| CN104755628B (zh) | 2012-09-25 | 2019-03-01 | 凯杰有限公司 | 生物样品的稳定化 |
| WO2014143961A1 (en) * | 2013-03-15 | 2014-09-18 | Regents Of The University Of Minnesota | Cryopreservative compositions and methods |
| CN105121643A (zh) | 2013-03-18 | 2015-12-02 | 凯杰有限公司 | 细胞外核酸的稳定化和分离 |
| EP2976424B1 (en) * | 2013-03-18 | 2018-10-03 | Qiagen GmbH | Stabilisation of biological samples |
| CN106686977B (zh) * | 2014-07-09 | 2022-03-25 | 豪夫迈·罗氏有限公司 | Ph调整以改善细胞库的解冻复苏 |
| AU2015339886B2 (en) * | 2014-09-29 | 2019-05-09 | Cook General Biotechnology Llc | Uses of trehalose in cell suspensions |
| CN104931611A (zh) * | 2015-06-03 | 2015-09-23 | 福建中烟工业有限责任公司 | 一种检测样品中肌醇的方法、试剂盒及其用途 |
| CN105230610B (zh) * | 2015-11-13 | 2017-10-27 | 中国科学院化学研究所 | 一种冷冻保存液及其制备方法和应用 |
| JP7166169B2 (ja) | 2015-11-20 | 2022-11-07 | キアゲン ゲーエムベーハー | 細胞外核酸の滅菌のための滅菌組成物を調製する方法 |
| WO2017143162A1 (en) | 2016-02-19 | 2017-08-24 | Regents Of University Of Minnesota | Cryoprotection compositions and methods |
| US11311008B2 (en) | 2016-04-19 | 2022-04-26 | Regents Of The University Of Minnesota. | Cryopreservation compositions and methods involving nanowarming |
| GB201608356D0 (en) * | 2016-05-12 | 2016-06-29 | Univ Leeds And Asymptote Ltd | Formulation |
| EP3487296A1 (en) * | 2016-07-22 | 2019-05-29 | Tissue Testing Technologies LLC | Enhancement of cell cryopreservation with glycolipids |
| WO2018227359A1 (en) * | 2017-06-13 | 2018-12-20 | Chung Chin Sun | A novel method for blood serum protein activity preservation |
| US20210137809A1 (en) * | 2017-06-14 | 2021-05-13 | Biosolution Co., Ltd | Cosmetic composition for wrinkle reduction or anti-inflammation, containing substance p |
| JP7072147B2 (ja) * | 2018-09-13 | 2022-05-20 | 極東製薬工業株式会社 | 間葉系幹細胞の凍害保護液とその利用 |
| JP7445611B2 (ja) * | 2019-02-15 | 2024-03-07 | イビデン株式会社 | 凍結保存液 |
| US12458016B2 (en) | 2019-02-28 | 2025-11-04 | Regents Of The University Of Minnesota | High throughput system for production and vitrification of biomaterials in cryoprotectant droplets |
| WO2021022112A1 (en) * | 2019-07-31 | 2021-02-04 | University Of South Carolina | Alginate-based microcapsulation for the delivery of alpha-cgrp in cardiovascular diseases |
| CN110720452B (zh) * | 2019-11-05 | 2021-11-19 | 南通大学 | 一种优化病理大体标本保存的方法 |
| CN110754464B (zh) * | 2019-11-13 | 2021-08-10 | 四川仟众生物科技有限公司 | 一种自体颅骨深低温保存方法 |
| KR102116954B1 (ko) * | 2019-12-26 | 2020-05-29 | 주식회사 엠케이바이오텍 | 태아 유래 줄기세포의 동결 보존 및 동결 건조용 배지 조성물 |
| CA3182658A1 (en) | 2020-05-08 | 2021-11-11 | Gallant Pet, Inc. | Uterine-derived regenerative cell compositions and uses thereof |
| WO2024110457A1 (en) * | 2022-11-21 | 2024-05-30 | Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E. V. | Identification of cryoprotectants for cryopreservation of cells and cell aggregates |
| CN117378598B (zh) * | 2023-12-08 | 2024-03-19 | 金宝医学科技(深圳)有限公司 | 一种卵母细胞冻存液及其制备方法 |
| CN118661721B (zh) * | 2024-08-26 | 2025-03-11 | 青岛宝迈得生物科技有限公司 | 一种低温冻存nk细胞的冻存液及其使用方法 |
Family Cites Families (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4690890A (en) * | 1984-04-04 | 1987-09-01 | Cetus Corporation | Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay |
| US6223064B1 (en) * | 1992-08-19 | 2001-04-24 | Lawrence A. Lynn | Microprocessor system for the simplified diagnosis of sleep apnea |
| US6342039B1 (en) * | 1992-08-19 | 2002-01-29 | Lawrence A. Lynn | Microprocessor system for the simplified diagnosis of sleep apnea |
| US6818199B1 (en) * | 1994-07-29 | 2004-11-16 | James F. Hainfeld | Media and methods for enhanced medical imaging |
| US5472749A (en) * | 1994-10-27 | 1995-12-05 | Northwestern University | Graphite encapsulated nanophase particles produced by a tungsten arc method |
| US5585020A (en) * | 1994-11-03 | 1996-12-17 | Becker; Michael F. | Process for the production of nanoparticles |
| US7011812B1 (en) * | 1996-05-03 | 2006-03-14 | Immunomedics, Inc. | Targeted combination immunotherapy of cancer and infectious diseases |
| IL120881A (en) * | 1996-07-30 | 2002-09-12 | It M R Medic L Cm 1997 Ltd | Method and device for continuous and non-invasive monitoring of peripheral arterial tone |
| US6103868A (en) * | 1996-12-27 | 2000-08-15 | The Regents Of The University Of California | Organically-functionalized monodisperse nanocrystals of metals |
| US6884842B2 (en) * | 1997-10-14 | 2005-04-26 | Alnis Biosciences, Inc. | Molecular compounds having complementary surfaces to targets |
| US6198281B1 (en) * | 1997-11-12 | 2001-03-06 | The Research Foundation Of State University Of New York | NMR spectroscopy of large proteins |
| US6370423B1 (en) * | 1998-10-05 | 2002-04-09 | Juan R. Guerrero | Method for analysis of biological voltage signals |
| US6142950A (en) * | 1998-12-10 | 2000-11-07 | Individual Monitoring Systems, Inc. | Non-tethered apnea screening device |
| US20070021979A1 (en) * | 1999-04-16 | 2007-01-25 | Cosentino Daniel L | Multiuser wellness parameter monitoring system |
| US6608562B1 (en) * | 1999-08-31 | 2003-08-19 | Denso Corporation | Vital signal detecting apparatus |
| US6480733B1 (en) * | 1999-11-10 | 2002-11-12 | Pacesetter, Inc. | Method for monitoring heart failure |
| US6409675B1 (en) * | 1999-11-10 | 2002-06-25 | Pacesetter, Inc. | Extravascular hemodynamic monitor |
| US6527729B1 (en) * | 1999-11-10 | 2003-03-04 | Pacesetter, Inc. | Method for monitoring patient using acoustic sensor |
| US7206636B1 (en) * | 1999-11-10 | 2007-04-17 | Pacesetter, Inc. | Pacing optimization based on changes in pulse amplitude and pulse amplitude variability |
| US6752765B1 (en) * | 1999-12-01 | 2004-06-22 | Medtronic, Inc. | Method and apparatus for monitoring heart rate and abnormal respiration |
| US6519490B1 (en) * | 1999-12-20 | 2003-02-11 | Joseph Wiesel | Method of and apparatus for detecting arrhythmia and fibrillation |
| US7806831B2 (en) * | 2000-03-02 | 2010-10-05 | Itamar Medical Ltd. | Method and apparatus for the non-invasive detection of particular sleep-state conditions by monitoring the peripheral vascular system |
| US6839581B1 (en) * | 2000-04-10 | 2005-01-04 | The Research Foundation Of State University Of New York | Method for detecting Cheyne-Stokes respiration in patients with congestive heart failure |
| WO2001076459A2 (en) * | 2000-04-10 | 2001-10-18 | The Research Foundation Of State University Of New York | Method for detecting cheyne-stokes respiration in patients with congestive heart failure |
| US6589188B1 (en) * | 2000-05-05 | 2003-07-08 | Pacesetter, Inc. | Method for monitoring heart failure via respiratory patterns |
| SG98393A1 (en) * | 2000-05-19 | 2003-09-19 | Inst Materials Research & Eng | Injectable drug delivery systems with cyclodextrin-polymer based hydrogels |
| US6480734B1 (en) * | 2000-06-30 | 2002-11-12 | Cardiac Science Inc. | Cardiac arrhythmia detector using ECG waveform-factor and its irregularity |
| US6856829B2 (en) * | 2000-09-07 | 2005-02-15 | Denso Corporation | Method for detecting physiological condition of sleeping patient based on analysis of pulse waves |
| SE0004417D0 (sv) * | 2000-11-28 | 2000-11-28 | St Jude Medical | Implantable device |
| US6529752B2 (en) * | 2001-01-17 | 2003-03-04 | David T. Krausman | Sleep disorder breathing event counter |
| US6918946B2 (en) * | 2001-07-02 | 2005-07-19 | Board Of Regents, The University Of Texas System | Applications of light-emitting nanoparticles |
| CA2466656C (en) * | 2001-11-09 | 2013-07-02 | Nanosphere, Inc. | Bioconjugate-nanoparticle probes |
| US20060177852A1 (en) * | 2001-12-12 | 2006-08-10 | Do-Coop Technologies Ltd. | Solid-fluid composition |
| US6829501B2 (en) * | 2001-12-20 | 2004-12-07 | Ge Medical Systems Information Technologies, Inc. | Patient monitor and method with non-invasive cardiac output monitoring |
| WO2003082077A2 (en) * | 2002-03-26 | 2003-10-09 | National Aeronautics And Space Administration | System for the diagnosis and monitoring of coronary artery disease, acute coronary syndromes, cardiomyopathy and other cardiac conditions |
| US6881192B1 (en) * | 2002-06-12 | 2005-04-19 | Pacesetter, Inc. | Measurement of sleep apnea duration and evaluation of response therapies using duration metrics |
| US6689342B1 (en) * | 2002-07-29 | 2004-02-10 | Warner-Lambert Company | Oral care compositions comprising tropolone compounds and essential oils and methods of using the same |
| US7024234B2 (en) * | 2002-09-20 | 2006-04-04 | Lyle Aaron Margulies | Method and apparatus for monitoring the autonomic nervous system |
| US7160252B2 (en) * | 2003-01-10 | 2007-01-09 | Medtronic, Inc. | Method and apparatus for detecting respiratory disturbances |
| AU2003901877A0 (en) * | 2003-04-16 | 2003-05-08 | Richard Charles Clark | Sleep management device |
| US7524292B2 (en) * | 2003-04-21 | 2009-04-28 | Medtronic, Inc. | Method and apparatus for detecting respiratory disturbances |
| US20050266090A1 (en) * | 2003-04-29 | 2005-12-01 | Ales Prokop | Nanoparticular targeting and therapy |
| IL155955A0 (en) * | 2003-05-15 | 2003-12-23 | Widemed Ltd | Adaptive prediction of changes of physiological/pathological states using processing of biomedical signal |
| KR20070028330A (ko) * | 2004-02-20 | 2007-03-12 | 두-쿱 테크놀로지스 리미티드 | 고체-유체 조성물 및 그의 용도 |
| US20050239108A1 (en) * | 2004-02-23 | 2005-10-27 | University Of Maryland, Baltimore | Immuno-PCR method for the detection of a biomolecule in a test sample |
| US20050191270A1 (en) * | 2004-02-27 | 2005-09-01 | Hydromer, Inc. | Anti-infectious hydrogel compositions |
| US7413549B1 (en) * | 2004-03-17 | 2008-08-19 | Pacesetter, Inc. | Detecting and quantifying apnea using ventilatory cycle histograms |
| JP3987053B2 (ja) * | 2004-03-30 | 2007-10-03 | 株式会社東芝 | 睡眠状態判定装置および睡眠状態判定方法 |
| DE102004016883A1 (de) * | 2004-04-06 | 2005-10-27 | Coripharm Medizinprodukte Gmbh & Co. Kg. | Verfahren zur Herstellung eines Knochen-Implantat-materials mit verbesserter mechanischer Beanspruchbarkeit auf der Basis von Formkörpern aus porösem Implantatmaterial sowie nach dem Verfahren hergestelltes Implantatmaterial |
| US7276088B2 (en) * | 2004-04-15 | 2007-10-02 | E.I. Du Pont De Nemours And Company | Hair coloring and cosmetic compositions comprising carbon nanotubes |
| US20070208269A1 (en) * | 2004-05-18 | 2007-09-06 | Mumford John R | Mask assembly, system and method for determining the occurrence of respiratory events using frontal electrode array |
| US7578793B2 (en) * | 2004-11-22 | 2009-08-25 | Widemed Ltd. | Sleep staging based on cardio-respiratory signals |
| US7680532B2 (en) * | 2005-02-25 | 2010-03-16 | Joseph Wiesel | Detecting atrial fibrillation, method of and apparatus for |
| US20070149870A1 (en) * | 2005-12-28 | 2007-06-28 | Futrex, Inc. | Systems and methods for determining an organism's pathology |
| US20090004296A1 (en) * | 2006-01-04 | 2009-01-01 | Do-Coop Technologies Ltd. | Antiseptic Compositions and Methods of Using Same |
| US7819816B2 (en) * | 2006-03-29 | 2010-10-26 | Cardiac Pacemakers, Inc. | Periodic disordered breathing detection |
| US20080003576A1 (en) * | 2006-06-30 | 2008-01-03 | Jingwu Zhang | Assay platforms and detection methodology using surface enhanced Raman scattering (SERS) upon specific biochemical interactions |
| US7972691B2 (en) * | 2006-12-22 | 2011-07-05 | Nanogram Corporation | Composites of polymers and metal/metalloid oxide nanoparticles and methods for forming these composites |
| KR20090095674A (ko) * | 2007-01-04 | 2009-09-09 | 두-쿱 테크놀로지스 리미티드 | 분석물의 검출 |
| WO2008081456A2 (en) * | 2007-01-04 | 2008-07-10 | Do-Coop Technologies Ltd. | Composition and method for enhancing cell growth and cell fusion |
| US20080300500A1 (en) * | 2007-05-30 | 2008-12-04 | Widemed Ltd. | Apnea detection using a capnograph |
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| AU2007203958A1 (en) | 2007-07-12 |
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| JP2009521949A (ja) | 2009-06-11 |
| KR20080098599A (ko) | 2008-11-11 |
| EP1981988A2 (en) | 2008-10-22 |
| KR20080098600A (ko) | 2008-11-11 |
| WO2007077560A2 (en) | 2007-07-12 |
| JP2009523128A (ja) | 2009-06-18 |
| US20090029340A1 (en) | 2009-01-29 |
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