CA2624916A1 - Production of oligodendrocytes from placenta-derived stem cells - Google Patents
Production of oligodendrocytes from placenta-derived stem cells Download PDFInfo
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- CA2624916A1 CA2624916A1 CA002624916A CA2624916A CA2624916A1 CA 2624916 A1 CA2624916 A1 CA 2624916A1 CA 002624916 A CA002624916 A CA 002624916A CA 2624916 A CA2624916 A CA 2624916A CA 2624916 A1 CA2624916 A1 CA 2624916A1
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- cells
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- placental stem
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- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Families Citing this family (79)
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| US8147824B2 (en) | 1999-08-05 | 2012-04-03 | Athersys, Inc. | Immunomodulatory properties of multipotent adult progenitor cells and uses thereof |
| MXPA03005014A (es) | 2000-12-06 | 2004-09-10 | Robert J Hariri | Metodo para recolectar celulas madres de la placenta. |
| US7311905B2 (en) * | 2002-02-13 | 2007-12-25 | Anthrogenesis Corporation | Embryonic-like stem cells derived from post-partum mammalian placenta, and uses and methods of treatment using said cells |
| AU2002251935B2 (en) | 2001-02-14 | 2007-11-29 | Celularity Inc. | Post-partum mammalian placenta, its use and placental stem cells therefrom |
| EP2338983B1 (en) | 2001-02-14 | 2015-10-07 | Anthrogenesis Corporation | Renovation and repopulation of decellularized tissues and cadaveric organs by stem cells |
| US7498171B2 (en) | 2002-04-12 | 2009-03-03 | Anthrogenesis Corporation | Modulation of stem and progenitor cell differentiation, assays, and uses thereof |
| AU2003298775B2 (en) * | 2002-11-26 | 2008-07-17 | Anthrogenesis Corporation | Cytotherapeutics, cytotherapeutic units and methods for treatments using them |
| JP2007531116A (ja) * | 2004-03-26 | 2007-11-01 | セルジーン・コーポレーション | 幹細胞バンクを提供するためのシステム及び方法 |
| US11660317B2 (en) | 2004-11-08 | 2023-05-30 | The Johns Hopkins University | Compositions comprising cardiosphere-derived cells for use in cell therapy |
| AU2006304274B2 (en) | 2005-10-13 | 2012-08-16 | Celularity Inc. | Production of oligodendrocytes from placenta-derived stem cells |
| EP1957633B1 (en) * | 2005-10-13 | 2013-12-18 | Anthrogenesis Corporation | Immunomodulation using placental stem cells |
| US10117900B2 (en) | 2005-11-09 | 2018-11-06 | Athersys, Inc. | MAPC treatment of brain injuries and diseases |
| US11000546B2 (en) | 2005-11-09 | 2021-05-11 | Athersys, Inc. | Immunomodulatory properties of MAPCs and uses thereof |
| AU2006332679A1 (en) * | 2005-12-29 | 2007-07-12 | Anthrogenesis Corporation | Co-culture of placental stem cells and stem cells from a second source |
| US9598669B2 (en) | 2005-12-29 | 2017-03-21 | Anthrogenesis Corporation | Composition for collecting placental stem cells and methods of using the composition |
| KR20180105266A (ko) | 2005-12-29 | 2018-09-27 | 안트로제네시스 코포레이션 | 태반 줄기 세포 집단 |
| CA3028321C (en) | 2006-01-23 | 2022-06-21 | Athersys, Inc. | Mapc treatment of brain injuries and diseases |
| WO2011132087A1 (en) * | 2010-04-23 | 2011-10-27 | Pluristem Ltd. | Adherent stromal cells derived from plancentas of multiple donors and uses thereof |
| CA2654716A1 (en) * | 2006-06-09 | 2007-12-21 | Anthrogenesis Corporation | Placental niche and use thereof to culture stem cells |
| US7993918B2 (en) * | 2006-08-04 | 2011-08-09 | Anthrogenesis Corporation | Tumor suppression using placental stem cells |
| US8372437B2 (en) | 2006-08-17 | 2013-02-12 | Mimedx Group, Inc. | Placental tissue grafts |
| NZ606814A (en) | 2006-10-23 | 2014-10-31 | Anthrogenesis Corp | Methods and compositions for treatment of bone defects with placental cell populations |
| JP2010518812A (ja) * | 2007-02-12 | 2010-06-03 | アンスロジェネシス コーポレーション | 接着性胎盤幹細胞由来の肝細胞および軟骨細胞、ならびにｃｄ３４＋、ｃｄ４５−胎盤幹細胞の濃縮細胞集団 |
| CN103356710A (zh) | 2007-02-12 | 2013-10-23 | 人类起源公司 | 利用胎盘干细胞治疗炎性疾病 |
| CA2688504A1 (en) * | 2007-06-18 | 2008-12-24 | Children's Hospital & Research Center At Oakland | Method of isolating stem and progenitor cells from placenta |
| US9200253B1 (en) | 2007-08-06 | 2015-12-01 | Anthrogenesis Corporation | Method of producing erythrocytes |
| EP2197270B8 (en) | 2007-09-07 | 2020-03-04 | Surgical Biologics, LLC | Placental tissue grafts and improved methods of preparing and using the same |
| ES2392729T5 (es) | 2007-09-19 | 2015-08-28 | Pluristem Ltd. | Células adherentes de tejidos adiposos o de placenta y uso de las mismas en terapia |
| EP2203176B1 (en) | 2007-09-28 | 2014-11-26 | Anthrogenesis Corporation | Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells |
| AU2008325142A1 (en) * | 2007-11-07 | 2009-05-14 | Anthrogenesis Corporation | Use of umbilical cord blood in the treatment of premature birth complications |
| US8338170B2 (en) | 2008-04-21 | 2012-12-25 | Viacyte, Inc. | Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells |
| CA2734237C (en) | 2008-08-20 | 2019-07-02 | Anthrogenesis Corporation | Treatment of stroke using isolated placental cells |
| NZ591292A (en) | 2008-08-20 | 2012-10-26 | Anthrogenesis Corp | Improved cell composition and methods of making the same |
| SI2331109T1 (sl) | 2008-08-22 | 2013-10-30 | Anthrogenesis Corporation | Postopki in sestavki za zdravljenje okvar kosti s populacijami placentarnih celic |
| EP2331957B1 (en) * | 2008-09-02 | 2016-11-02 | Pluristem Ltd. | Methods of selection of cells for transplantation |
| WO2010059828A1 (en) | 2008-11-19 | 2010-05-27 | Anthrogenesis Corporation | Amnion derived adherent cells |
| US8771677B2 (en) | 2008-12-29 | 2014-07-08 | Vladimir B Serikov | Colony-forming unit cell of human chorion and method to obtain and use thereof |
| EP2449095A1 (en) * | 2009-07-02 | 2012-05-09 | Anthrogenesis Corporation | Method of producing erythrocytes without feeder cells |
| CA2787992A1 (en) | 2010-01-26 | 2011-08-04 | Anthrogenesis Corporation | Treatment of bone-related cancers using placental stem cells |
| WO2011103462A1 (en) | 2010-02-18 | 2011-08-25 | Osiris Therapeutics, Inc. | Methods of manufacture of immunocompatible amniotic membrane products |
| CN102191229A (zh) * | 2010-03-16 | 2011-09-21 | 侯亚义 | 一种快速有效获得脐带间充质干细胞(msc)的方法 |
| PL2556145T3 (pl) | 2010-04-07 | 2017-02-28 | Anthrogenesis Corporation | Angiogeneza z zastosowaniem łożyskowych komórek macierzystych |
| KR20130092394A (ko) | 2010-04-08 | 2013-08-20 | 안트로제네시스 코포레이션 | 태반 줄기 세포를 사용한 사르코이드증의 치료 |
| US9845457B2 (en) * | 2010-04-30 | 2017-12-19 | Cedars-Sinai Medical Center | Maintenance of genomic stability in cultured stem cells |
| US9352003B1 (en) | 2010-05-14 | 2016-05-31 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US10130736B1 (en) | 2010-05-14 | 2018-11-20 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US8883210B1 (en) | 2010-05-14 | 2014-11-11 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| MX342995B (es) | 2010-07-13 | 2016-10-21 | Anthrogenesis Corp | Métodos de generar células asesinas naturales. |
| US8969315B2 (en) | 2010-12-31 | 2015-03-03 | Anthrogenesis Corporation | Enhancement of placental stem cell potency using modulatory RNA molecules |
| DK2714059T3 (en) | 2011-06-01 | 2019-01-21 | Celularity Inc | TREATMENT OF PAIN WHEN USING PLACENT STAM CELLS |
| WO2012168295A1 (en) * | 2011-06-06 | 2012-12-13 | ReGenesys BVBA | Expansion of stem cells in hollow fiber bioreactors |
| US10190092B2 (en) | 2011-08-11 | 2019-01-29 | Robert A. Dracker | Procurement of placental stem cells |
| US20130040281A1 (en) * | 2011-08-11 | 2013-02-14 | Robert A. Dracker | Procurement of Placental Stem Cells |
| WO2013055476A1 (en) | 2011-09-09 | 2013-04-18 | Anthrogenesis Corporation | Treatment of amyotrophic lateral sclerosis using placental stem cells |
| EP2760478A1 (en) * | 2011-09-26 | 2014-08-06 | Universität Zürich Prorektorat MNW | Apc-mediated tolerance induction for therapy of multiple sclerosis |
| EP3563859B1 (en) | 2012-08-13 | 2021-10-13 | Cedars-Sinai Medical Center | Cardiosphere-derived exosomes for tissue regeneration |
| EP3622960A1 (en) | 2013-02-05 | 2020-03-18 | Celularity, Inc. | Natural killer cells from placenta |
| CN105408472A (zh) | 2013-03-15 | 2016-03-16 | 马卡斯凯尔·托莱夫·拉尔森 | 用于脐带血收集及细胞分离的细胞、方法和装置 |
| US20160326487A1 (en) | 2014-01-08 | 2016-11-10 | Samsung Life Public Welfare Foundation | Stem Cells Derived from Pure Chorionic Trophoblast Layer and Cell Therapy Comprising Same |
| WO2016044421A1 (en) * | 2014-09-18 | 2016-03-24 | National Health Research Institutes | Progenitor cells, method for preparation thereof and uses thereof |
| CA2962444C (en) | 2014-10-03 | 2023-09-05 | Cedars-Sinai Medical Center | Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of muscular dystrophy |
| CN104774807B (zh) * | 2015-04-28 | 2018-05-08 | 安沂华 | 将脐带间充质干细胞诱导分化成少突胶质细胞的方法 |
| CA2986702C (en) | 2015-05-21 | 2023-04-04 | David Wang | Modified demineralized cortical bone fibers |
| US12551509B2 (en) | 2015-11-18 | 2026-02-17 | Lucina Patent Holdco, Llc | Acellular regenerative products and methods of their manufacture |
| EP3402543B1 (en) | 2016-01-11 | 2021-09-08 | Cedars-Sinai Medical Center | Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of heart failure with preserved ejection fraction |
| US11351200B2 (en) | 2016-06-03 | 2022-06-07 | Cedars-Sinai Medical Center | CDC-derived exosomes for treatment of ventricular tachyarrythmias |
| WO2018057542A1 (en) | 2016-09-20 | 2018-03-29 | Cedars-Sinai Medical Center | Cardiosphere-derived cells and their extracellular vesicles to retard or reverse aging and age-related disorders |
| US11542473B2 (en) | 2016-10-21 | 2023-01-03 | Amniotics Ab | Methods and compositions for generating hematopoietic cells |
| EP3612191B1 (en) | 2017-04-19 | 2026-03-11 | Cedars-Sinai Medical Center | Methods and compositions for treating skeletal muscular dystrophy |
| EP3412294A1 (en) * | 2017-06-08 | 2018-12-12 | Universite De Fribourg | Hdac1/2 activator for promoting and/or accelerating myelination and/or remyelination |
| CN107460169B (zh) * | 2017-07-14 | 2020-12-11 | 中国科学院广州生物医药与健康研究院 | 维生素c在制备培养基中的用途 |
| US11660355B2 (en) | 2017-12-20 | 2023-05-30 | Cedars-Sinai Medical Center | Engineered extracellular vesicles for enhanced tissue delivery |
| EP3749344A4 (en) | 2018-02-05 | 2022-01-26 | Cedars-Sinai Medical Center | METHODS OF THERAPEUTIC USE OF EXOSOMES AND Y-RNAS |
| CN108220222A (zh) * | 2018-04-19 | 2018-06-29 | 重庆斯德姆生物技术有限公司 | 一种细胞分散剂 |
| WO2020256628A1 (en) | 2019-06-20 | 2020-12-24 | Amniotics Ab | An apparatus for filtering amniotic fluid |
| JP7714535B2 (ja) | 2019-10-18 | 2025-07-29 | アムニオティクス・アーベー | 羊水およびそれに由来する細胞から羊水間葉系幹細胞を得るためのプロセスならびに装置 |
| KR102400263B1 (ko) * | 2020-04-08 | 2022-05-20 | 이엔셀 주식회사 | 줄기세포 초기 수율 촉진을 위한 줄기세포 배양 방법 |
| TR202006919A2 (tr) * | 2020-05-04 | 2021-11-22 | Univ Yeditepe | Di̇ş kök hücresi̇nden nöron hücresi̇ farklilaştirmasi amaciyla gerçekleşti̇ri̇len besi̇yeri̇ tabanli bi̇r yöntem |
| US12435308B2 (en) | 2020-11-06 | 2025-10-07 | Amniotics Ab | Immunomodulation by amniotic fluid mesenchymal stem cells |
Family Cites Families (66)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4798824A (en) | 1985-10-03 | 1989-01-17 | Wisconsin Alumni Research Foundation | Perfusate for the preservation of organs |
| US5190556A (en) | 1991-03-19 | 1993-03-02 | O.B. Tech, Inc. | Cord cutter sampler |
| US5552267A (en) | 1992-04-03 | 1996-09-03 | The Trustees Of Columbia University In The City Of New York | Solution for prolonged organ preservation |
| US5372581A (en) | 1993-07-21 | 1994-12-13 | Minneapolis Children's Services Corporation | Method and apparatus for placental blood collection |
| WO2000063268A1 (en) | 1999-04-16 | 2000-10-26 | Wm. Marsh Rice University | Poly(propylene fumarate) cross linked with poly(ethylene glycol) |
| US6355699B1 (en) | 1999-06-30 | 2002-03-12 | Ethicon, Inc. | Process for manufacturing biomedical foams |
| US7311905B2 (en) * | 2002-02-13 | 2007-12-25 | Anthrogenesis Corporation | Embryonic-like stem cells derived from post-partum mammalian placenta, and uses and methods of treatment using said cells |
| US20080152629A1 (en) * | 2000-12-06 | 2008-06-26 | James Edinger | Placental stem cell populations |
| MXPA03005014A (es) | 2000-12-06 | 2004-09-10 | Robert J Hariri | Metodo para recolectar celulas madres de la placenta. |
| AU2002251935B2 (en) * | 2001-02-14 | 2007-11-29 | Celularity Inc. | Post-partum mammalian placenta, its use and placental stem cells therefrom |
| EP2338983B1 (en) | 2001-02-14 | 2015-10-07 | Anthrogenesis Corporation | Renovation and repopulation of decellularized tissues and cadaveric organs by stem cells |
| ES2387082T3 (es) | 2001-05-17 | 2012-09-13 | Merck Serono Sa | Uso de OSTEOPONTINA para el tratamiento y/o prevención de enfermedades neurológicas |
| US7799324B2 (en) * | 2001-12-07 | 2010-09-21 | Geron Corporation | Using undifferentiated embryonic stem cells to control the immune system |
| EP1482787A4 (en) | 2002-02-13 | 2006-02-15 | Anthrogenesis Corp | EMBRYONIC TYPE DERIVED STEM CELLS DERIVED FROM MAMMALIAN POST-PARTUM PLACENTA, USES THEREOF, AND METHODS OF TREATMENT BASED ON CELLS OF THIS TYPE |
| US20030187515A1 (en) * | 2002-03-26 | 2003-10-02 | Hariri Robert J. | Collagen biofabric and methods of preparing and using the collagen biofabric |
| US7498171B2 (en) * | 2002-04-12 | 2009-03-03 | Anthrogenesis Corporation | Modulation of stem and progenitor cell differentiation, assays, and uses thereof |
| CA2481385A1 (en) * | 2002-04-12 | 2003-10-23 | Celgene Corporation | Modulation of stem and progenitor cell differentiation, assays, and uses thereof |
| CN1658848A (zh) * | 2002-04-12 | 2005-08-24 | 细胞基因公司 | 血管生成调节剂的鉴定方法,由此发现的化合物和使用该化合物的治疗方法 |
| US20040161419A1 (en) * | 2002-04-19 | 2004-08-19 | Strom Stephen C. | Placental stem cells and uses thereof |
| CA2483010A1 (en) * | 2002-04-19 | 2003-10-30 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Placental derived stem cells and uses thereof |
| MXPA04011851A (es) | 2002-05-30 | 2005-03-31 | Celgene Corp | Metodos para utilizar inhibidores de jnk o mkk para modular diferenciacion de celula y para tratar desordenes mieloproliferativos y sindromes mielodispl??sticos. |
| US7285415B2 (en) * | 2002-07-11 | 2007-10-23 | The Regents Of The University Of California | Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury |
| AU2003298775B2 (en) | 2002-11-26 | 2008-07-17 | Anthrogenesis Corporation | Cytotherapeutics, cytotherapeutic units and methods for treatments using them |
| NZ566132A (en) * | 2003-02-13 | 2009-09-25 | Anthrogenesis Corp | Use of umbilical cord blood to treat inflammation, ParkinsonÆs disease or diabetes |
| US7253166B2 (en) * | 2003-04-22 | 2007-08-07 | Irm Llc | 6-phenyl-7H-pyrrolo[2,3-d]pyrimidine compounds that induce neuronal differentiation in embryonic stem cells |
| ES2569780T3 (es) * | 2003-06-27 | 2016-05-12 | DePuy Synthes Products, Inc. | Células posparto derivadas de tejido placentario y métodos de preparación y uso de las mismas |
| US8426200B2 (en) * | 2003-07-02 | 2013-04-23 | Regents Of The University Of Minnesota | Neuronal differentiation of stem cells |
| CN1886149A (zh) * | 2003-09-23 | 2006-12-27 | 路德维格癌症研究院 | Vegf－c或vegf－d物质及刺激神经干细胞的方法 |
| BRPI0417186A (pt) * | 2003-12-02 | 2007-03-06 | Celgene Corp | método de tratamento de um indivìduo tendo uma hemoglobinopatia ou uma anemia, de modulação da diferenciação de uma célula-tronco ou precursora de cd34+ para uma linhagem de eritróides, e, composição farmacêutica |
| US20050176139A1 (en) * | 2004-01-12 | 2005-08-11 | Yao-Chang Chen | Placental stem cell and methods thereof |
| US20050266391A1 (en) * | 2004-01-15 | 2005-12-01 | Bennett Brydon L | Methods for preserving tissue |
| JP2007531116A (ja) * | 2004-03-26 | 2007-11-01 | セルジーン・コーポレーション | 幹細胞バンクを提供するためのシステム及び方法 |
| US7244759B2 (en) * | 2004-07-28 | 2007-07-17 | Celgene Corporation | Isoindoline compounds and methods of making and using the same |
| US7909806B2 (en) * | 2004-09-23 | 2011-03-22 | Anthrogenesis Corporation | Cord blood and placenta collection kit |
| US7147626B2 (en) * | 2004-09-23 | 2006-12-12 | Celgene Corporation | Cord blood and placenta collection kit |
| ZA200800144B (en) * | 2005-06-10 | 2010-02-24 | Celgene Corp | Human placental collagen compositions, processes for their preparation, methods of their use and kits comprising the compositions |
| AU2006265601A1 (en) * | 2005-06-30 | 2007-01-11 | Anthrogenesis Corporation | Repair of tympanic membrane using placenta derived collagen biofabric |
| US20070021762A1 (en) * | 2005-07-13 | 2007-01-25 | Qing Liu | Ocular plug formed from placenta derived collagen biofabric |
| WO2007009062A2 (en) * | 2005-07-13 | 2007-01-18 | Anthrogenesis Corporation | Treatment of leg ulcers using placenta derived collagen biofabric |
| AU2006304274B2 (en) | 2005-10-13 | 2012-08-16 | Celularity Inc. | Production of oligodendrocytes from placenta-derived stem cells |
| EP1957633B1 (en) * | 2005-10-13 | 2013-12-18 | Anthrogenesis Corporation | Immunomodulation using placental stem cells |
| US9598669B2 (en) * | 2005-12-29 | 2017-03-21 | Anthrogenesis Corporation | Composition for collecting placental stem cells and methods of using the composition |
| KR20180105266A (ko) * | 2005-12-29 | 2018-09-27 | 안트로제네시스 코포레이션 | 태반 줄기 세포 집단 |
| AU2006332679A1 (en) * | 2005-12-29 | 2007-07-12 | Anthrogenesis Corporation | Co-culture of placental stem cells and stem cells from a second source |
| CA2654716A1 (en) * | 2006-06-09 | 2007-12-21 | Anthrogenesis Corporation | Placental niche and use thereof to culture stem cells |
| US7993918B2 (en) * | 2006-08-04 | 2011-08-09 | Anthrogenesis Corporation | Tumor suppression using placental stem cells |
| WO2008021391A1 (en) * | 2006-08-15 | 2008-02-21 | Anthrogenesis Corporation | Umbilical cord biomaterial for medical use |
| US8122763B2 (en) * | 2006-09-01 | 2012-02-28 | Avair, Llc | Breathing gas supply visual broadcast apparatus |
| US20080131522A1 (en) * | 2006-10-03 | 2008-06-05 | Qing Liu | Use of placental biomaterial for ocular surgery |
| US8071135B2 (en) * | 2006-10-04 | 2011-12-06 | Anthrogenesis Corporation | Placental tissue compositions |
| AU2007318210B2 (en) * | 2006-10-06 | 2014-02-20 | Celularity Inc. | Native (telopeptide) placental collagen compositions |
| NZ606814A (en) * | 2006-10-23 | 2014-10-31 | Anthrogenesis Corp | Methods and compositions for treatment of bone defects with placental cell populations |
| CN103356710A (zh) * | 2007-02-12 | 2013-10-23 | 人类起源公司 | 利用胎盘干细胞治疗炎性疾病 |
| JP2010518812A (ja) * | 2007-02-12 | 2010-06-03 | アンスロジェネシス コーポレーション | 接着性胎盤幹細胞由来の肝細胞および軟骨細胞、ならびにｃｄ３４＋、ｃｄ４５−胎盤幹細胞の濃縮細胞集団 |
| US20100172830A1 (en) * | 2007-03-29 | 2010-07-08 | Cellx Inc. | Extraembryonic Tissue cells and method of use thereof |
| KR20200136051A (ko) * | 2007-09-26 | 2020-12-04 | 안트로제네시스 코포레이션 | 인간 태반 관류액으로부터의 혈관형성 세포 |
| EP2203176B1 (en) * | 2007-09-28 | 2014-11-26 | Anthrogenesis Corporation | Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells |
| AU2008325142A1 (en) * | 2007-11-07 | 2009-05-14 | Anthrogenesis Corporation | Use of umbilical cord blood in the treatment of premature birth complications |
| CA2734237C (en) * | 2008-08-20 | 2019-07-02 | Anthrogenesis Corporation | Treatment of stroke using isolated placental cells |
| NZ591292A (en) * | 2008-08-20 | 2012-10-26 | Anthrogenesis Corp | Improved cell composition and methods of making the same |
| SI2331109T1 (sl) * | 2008-08-22 | 2013-10-30 | Anthrogenesis Corporation | Postopki in sestavki za zdravljenje okvar kosti s populacijami placentarnih celic |
| WO2010059828A1 (en) * | 2008-11-19 | 2010-05-27 | Anthrogenesis Corporation | Amnion derived adherent cells |
| EP2375907B1 (en) * | 2008-11-21 | 2019-02-27 | Celularity, Inc. | Treatment of diseases, disorders or conditions of the lung using placental cells |
| WO2010141654A1 (en) * | 2009-06-05 | 2010-12-09 | Anthrogenesis Corporation | Improved method of collecting placental cells |
| EP2449095A1 (en) * | 2009-07-02 | 2012-05-09 | Anthrogenesis Corporation | Method of producing erythrocytes without feeder cells |
| CA2787992A1 (en) * | 2010-01-26 | 2011-08-04 | Anthrogenesis Corporation | Treatment of bone-related cancers using placental stem cells |
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