CA2615468C - Chimeric empty viral-like particles derived from the infectious bursal disease virus (ibdv), process for their production and applications - Google Patents
Chimeric empty viral-like particles derived from the infectious bursal disease virus (ibdv), process for their production and applications Download PDFInfo
- Publication number
- CA2615468C CA2615468C CA2615468A CA2615468A CA2615468C CA 2615468 C CA2615468 C CA 2615468C CA 2615468 A CA2615468 A CA 2615468A CA 2615468 A CA2615468 A CA 2615468A CA 2615468 C CA2615468 C CA 2615468C
- Authority
- CA
- Canada
- Prior art keywords
- pvp2
- ibdv
- region
- protein
- fusion protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000702626 Infectious bursal disease virus Species 0.000 title claims abstract description 238
- 239000002245 particle Substances 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims description 35
- 238000004519 manufacturing process Methods 0.000 title claims description 6
- 230000008569 process Effects 0.000 title description 21
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 190
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 184
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 122
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 102
- 229920001184 polypeptide Polymers 0.000 claims abstract description 97
- 108020001507 fusion proteins Proteins 0.000 claims abstract description 94
- 102000037865 fusion proteins Human genes 0.000 claims abstract description 94
- 239000012634 fragment Substances 0.000 claims abstract description 55
- 210000004027 cell Anatomy 0.000 claims description 67
- 150000007523 nucleic acids Chemical class 0.000 claims description 40
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 32
- 108020004707 nucleic acids Proteins 0.000 claims description 23
- 102000039446 nucleic acids Human genes 0.000 claims description 23
- 229960005486 vaccine Drugs 0.000 claims description 21
- 241000700605 Viruses Species 0.000 claims description 18
- 241000238631 Hexapoda Species 0.000 claims description 14
- 201000010099 disease Diseases 0.000 claims description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 12
- 239000008194 pharmaceutical composition Substances 0.000 claims description 11
- 241000701447 unidentified baculovirus Species 0.000 claims description 11
- 239000002671 adjuvant Substances 0.000 claims description 9
- 230000028993 immune response Effects 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- 125000000539 amino acid group Chemical group 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 6
- 206010028980 Neoplasm Diseases 0.000 claims description 5
- 244000045947 parasite Species 0.000 claims description 5
- 238000002255 vaccination Methods 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 4
- 238000003745 diagnosis Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000002560 therapeutic procedure Methods 0.000 claims description 4
- 210000005253 yeast cell Anatomy 0.000 claims description 4
- 239000000411 inducer Substances 0.000 claims description 3
- 230000000890 antigenic effect Effects 0.000 claims description 2
- 230000001225 therapeutic effect Effects 0.000 abstract description 6
- 230000000069 prophylactic effect Effects 0.000 abstract description 2
- 235000018102 proteins Nutrition 0.000 description 174
- 210000000234 capsid Anatomy 0.000 description 102
- 101710081079 Minor spike protein H Proteins 0.000 description 42
- 239000002773 nucleotide Substances 0.000 description 42
- 125000003729 nucleotide group Chemical group 0.000 description 42
- 230000014509 gene expression Effects 0.000 description 40
- 239000013598 vector Substances 0.000 description 39
- 235000001014 amino acid Nutrition 0.000 description 28
- 150000001413 amino acids Chemical class 0.000 description 28
- 210000004899 c-terminal region Anatomy 0.000 description 26
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 24
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 23
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 23
- 101000805768 Banna virus (strain Indonesia/JKT-6423/1980) mRNA (guanine-N(7))-methyltransferase Proteins 0.000 description 22
- 101000686790 Chaetoceros protobacilladnavirus 2 Replication-associated protein Proteins 0.000 description 22
- 101000864475 Chlamydia phage 1 Internal scaffolding protein VP3 Proteins 0.000 description 22
- 101000803553 Eumenes pomiformis Venom peptide 3 Proteins 0.000 description 22
- 101000583961 Halorubrum pleomorphic virus 1 Matrix protein Proteins 0.000 description 22
- 108091028043 Nucleic acid sequence Proteins 0.000 description 22
- 108700026244 Open Reading Frames Proteins 0.000 description 21
- 230000000712 assembly Effects 0.000 description 17
- 238000000429 assembly Methods 0.000 description 17
- 230000015572 biosynthetic process Effects 0.000 description 17
- 239000013612 plasmid Substances 0.000 description 16
- 238000004458 analytical method Methods 0.000 description 15
- 238000005755 formation reaction Methods 0.000 description 14
- 241001465754 Metazoa Species 0.000 description 13
- 101710132601 Capsid protein Proteins 0.000 description 12
- 101710197658 Capsid protein VP1 Proteins 0.000 description 12
- 101710118046 RNA-directed RNA polymerase Proteins 0.000 description 12
- 101710108545 Viral protein 1 Proteins 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 11
- 229930006000 Sucrose Natural products 0.000 description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 11
- 238000001493 electron microscopy Methods 0.000 description 11
- 239000005720 sucrose Substances 0.000 description 11
- 239000013638 trimer Substances 0.000 description 11
- 230000003612 virological effect Effects 0.000 description 11
- 108010076039 Polyproteins Proteins 0.000 description 10
- 239000013543 active substance Substances 0.000 description 10
- 238000012217 deletion Methods 0.000 description 10
- 230000037430 deletion Effects 0.000 description 10
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 10
- 238000001262 western blot Methods 0.000 description 10
- 238000001415 gene therapy Methods 0.000 description 9
- 238000012545 processing Methods 0.000 description 9
- 210000002966 serum Anatomy 0.000 description 9
- 208000015181 infectious disease Diseases 0.000 description 8
- 230000003993 interaction Effects 0.000 description 8
- 101800001319 Capsid protein VP3 Proteins 0.000 description 7
- 239000000872 buffer Substances 0.000 description 7
- 239000000969 carrier Substances 0.000 description 7
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 7
- 239000000499 gel Substances 0.000 description 7
- 238000001000 micrograph Methods 0.000 description 7
- 239000002953 phosphate buffered saline Substances 0.000 description 7
- 239000002243 precursor Substances 0.000 description 7
- 241000271566 Aves Species 0.000 description 6
- 108020004414 DNA Proteins 0.000 description 6
- 108060004795 Methyltransferase Proteins 0.000 description 6
- 238000002983 circular dichroism Methods 0.000 description 6
- 229940079593 drug Drugs 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 238000013518 transcription Methods 0.000 description 6
- 230000035897 transcription Effects 0.000 description 6
- 241000699670 Mus sp. Species 0.000 description 5
- 230000003053 immunization Effects 0.000 description 5
- 238000002649 immunization Methods 0.000 description 5
- 239000000543 intermediate Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 241000282414 Homo sapiens Species 0.000 description 4
- 241000286209 Phasianidae Species 0.000 description 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 4
- 210000001744 T-lymphocyte Anatomy 0.000 description 4
- RHQDFWAXVIIEBN-UHFFFAOYSA-N Trifluoroethanol Chemical compound OCC(F)(F)F RHQDFWAXVIIEBN-UHFFFAOYSA-N 0.000 description 4
- 239000000427 antigen Substances 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 210000003719 b-lymphocyte Anatomy 0.000 description 4
- 230000000903 blocking effect Effects 0.000 description 4
- 238000012512 characterization method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000013613 expression plasmid Substances 0.000 description 4
- 235000014304 histidine Nutrition 0.000 description 4
- 239000005556 hormone Substances 0.000 description 4
- 229940088597 hormone Drugs 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000002797 proteolythic effect Effects 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 3
- 229920001213 Polysorbate 20 Polymers 0.000 description 3
- -1 antibodies Substances 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 108091006116 chimeric peptides Proteins 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 238000000635 electron micrograph Methods 0.000 description 3
- 230000002458 infectious effect Effects 0.000 description 3
- 230000001788 irregular Effects 0.000 description 3
- 125000005647 linker group Chemical group 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 230000008520 organization Effects 0.000 description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 230000001131 transforming effect Effects 0.000 description 3
- 239000011534 wash buffer Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 208000031295 Animal disease Diseases 0.000 description 2
- 101500001532 Avian infectious bursal disease virus Capsid protein VP2 Proteins 0.000 description 2
- 101500001538 Avian infectious bursal disease virus Capsid protein VP3 Proteins 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 241000588722 Escherichia Species 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- 241000700588 Human alphaherpesvirus 1 Species 0.000 description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
- 101710091437 Major capsid protein 2 Proteins 0.000 description 2
- 108010021466 Mutant Proteins Proteins 0.000 description 2
- 102000008300 Mutant Proteins Human genes 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 108020005091 Replication Origin Proteins 0.000 description 2
- 108700010756 Viral Polyproteins Proteins 0.000 description 2
- 230000019552 anatomical structure morphogenesis Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 210000004507 artificial chromosome Anatomy 0.000 description 2
- 210000001106 artificial yeast chromosome Anatomy 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000013330 chicken meat Nutrition 0.000 description 2
- 238000001142 circular dichroism spectrum Methods 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000004590 computer program Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 150000002411 histidines Chemical class 0.000 description 2
- 230000002163 immunogen Effects 0.000 description 2
- 230000005847 immunogenicity Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000012678 infectious agent Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 239000000902 placebo Substances 0.000 description 2
- 229940068196 placebo Drugs 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 210000005239 tubule Anatomy 0.000 description 2
- 210000002845 virion Anatomy 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 1
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 1
- JYCQQPHGFMYQCF-UHFFFAOYSA-N 4-tert-Octylphenol monoethoxylate Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCO)C=C1 JYCQQPHGFMYQCF-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000272517 Anseriformes Species 0.000 description 1
- 101500001537 Avian infectious bursal disease virus Protease VP4 Proteins 0.000 description 1
- 241000702628 Birnaviridae Species 0.000 description 1
- 206010006811 Bursitis Diseases 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 108090000565 Capsid Proteins Proteins 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 102100023321 Ceruloplasmin Human genes 0.000 description 1
- 238000011537 Coomassie blue staining Methods 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 241000275449 Diplectrum formosum Species 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 238000012286 ELISA Assay Methods 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000702191 Escherichia virus P1 Species 0.000 description 1
- 241000701533 Escherichia virus T4 Species 0.000 description 1
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000018713 Histocompatibility Antigens Class II Human genes 0.000 description 1
- 108010027412 Histocompatibility Antigens Class II Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 241000235058 Komagataella pastoris Species 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 241000222722 Leishmania <genus> Species 0.000 description 1
- 101000679474 Leishmania mexicana Triosephosphate isomerase Proteins 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 101710136297 Protein VP2 Proteins 0.000 description 1
- 241000125945 Protoparvovirus Species 0.000 description 1
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 235000004443 Ricinus communis Nutrition 0.000 description 1
- 241000702670 Rotavirus Species 0.000 description 1
- 108091006629 SLC13A2 Proteins 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 101100483030 Salmonella phage ViI tail sheath gene Proteins 0.000 description 1
- 101710184528 Scaffolding protein Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000271567 Struthioniformes Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 101000865057 Thermococcus litoralis DNA polymerase Proteins 0.000 description 1
- 102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 1
- 108700015934 Triose-phosphate isomerases Proteins 0.000 description 1
- 102100033598 Triosephosphate isomerase Human genes 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 239000006035 Tryptophane Substances 0.000 description 1
- 102000004243 Tubulin Human genes 0.000 description 1
- 108090000704 Tubulin Proteins 0.000 description 1
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical group O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 1
- COQLPRJCUIATTQ-UHFFFAOYSA-N Uranyl acetate Chemical compound O.O.O=[U]=O.CC(O)=O.CC(O)=O COQLPRJCUIATTQ-UHFFFAOYSA-N 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 201000009310 astigmatism Diseases 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000012677 causal agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000009850 completed effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 235000012489 doughnuts Nutrition 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- CCIVGXIOQKPBKL-UHFFFAOYSA-N ethanesulfonic acid Chemical compound CCS(O)(=O)=O CCIVGXIOQKPBKL-UHFFFAOYSA-N 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 101710121537 mRNA (guanine-N(7))-methyltransferase Proteins 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 125000000325 methylidene group Chemical group [H]C([H])=* 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 238000000302 molecular modelling Methods 0.000 description 1
- 208000030194 mouth disease Diseases 0.000 description 1
- 229940031348 multivalent vaccine Drugs 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 238000006384 oligomerization reaction Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 239000007261 sc medium Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229940031626 subunit vaccine Drugs 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 229960001295 tocopherol Drugs 0.000 description 1
- 229930003799 tocopherol Natural products 0.000 description 1
- 235000010384 tocopherol Nutrition 0.000 description 1
- 239000011732 tocopherol Substances 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 230000010415 tropism Effects 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- C12N7/04—Inactivation or attenuation; Producing viral sub-units
- C12N7/045—Pseudoviral particles; Non infectious pseudovirions, e.g. genetically engineered
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5256—Virus expressing foreign proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5258—Virus-like particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/10011—Birnaviridae
- C12N2720/10023—Virus like particles [VLP]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ESP200501733 | 2005-07-15 | ||
| ES200501733A ES2310062B1 (es) | 2005-07-15 | 2005-07-15 | Particulas pseudovirales vacias quimericas derivadas del virus causante de la enfermedad de la bursitis infecciosa (ibdv), procedimiento de obtencion y aplicaciones. |
| PCT/EP2006/006915 WO2007009673A1 (en) | 2005-07-15 | 2006-07-14 | Chimeric empty viral-like particles derived from the infectious bursal disease virus (ibdv), process for their production and applications |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2615468A1 CA2615468A1 (en) | 2007-01-25 |
| CA2615468C true CA2615468C (en) | 2015-04-14 |
Family
ID=37662111
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2615468A Active CA2615468C (en) | 2005-07-15 | 2006-07-14 | Chimeric empty viral-like particles derived from the infectious bursal disease virus (ibdv), process for their production and applications |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7476387B2 (enExample) |
| EP (1) | EP1904626B1 (enExample) |
| JP (1) | JP5713533B2 (enExample) |
| CN (1) | CN101278043A (enExample) |
| AT (1) | ATE516345T1 (enExample) |
| AU (1) | AU2006271978B2 (enExample) |
| CA (1) | CA2615468C (enExample) |
| ES (2) | ES2310062B1 (enExample) |
| WO (1) | WO2007009673A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2093281A1 (en) * | 2008-02-19 | 2009-08-26 | Kapsid Link, S.L. | Protein nanocarriers, process for obtaining them and applications |
| EP2288618B1 (en) * | 2008-05-09 | 2017-09-20 | Chimera Pharma S.l.u. | Chimeric fusion proteins and virus like particles from birnavirus vp2 |
| WO2011054995A2 (es) | 2009-11-06 | 2011-05-12 | Chimera Pharma, S. L. U. | VACUNAS PROFILACTICAS DE GRIPE A PARTIR DE CAPSIDAS VIRALES DE BIRNAVIRUS CONTENIENDO EL ANTIGENO M2e DEL VIRUS DE LA GRIPE |
| JP5795319B2 (ja) * | 2009-11-06 | 2015-10-14 | チャイメラ ファーマ エス．エル．ユー．Ｃｈｉｍｅｒａ Ｐｈａｒｍａ Ｓ．Ｌ．Ｕ． | ヒトパピローマウイルス抗原を含むビルナウイルスのウイルスカプシドに基づく腫瘍形成を処置するためのワクチン |
| CN104093420B (zh) | 2011-11-03 | 2017-07-28 | 森提耐斯特治疗公司 | 针对人肠道病毒的抗原及疫苗 |
| WO2014008475A2 (en) | 2012-07-05 | 2014-01-09 | The Ohio State University | Compositions and methods related to viral vaccines |
| US10086062B2 (en) | 2012-07-05 | 2018-10-02 | Ohio State Innovation Foundation | Infectious pancreatic necrosis virus (IPNV) vaccine compositions |
| EP3089755A1 (en) * | 2014-01-03 | 2016-11-09 | Fundacion Biofisica Bizkaia | VLPs, METHODS FOR THEIR OBTENTION AND APPLICATIONS THEREOF |
| CN107034198B (zh) * | 2015-07-15 | 2021-03-09 | 长春百克生物科技股份公司 | 一种嵌合诺如病毒p颗粒及其制备和应用 |
| CN108624611A (zh) * | 2018-05-15 | 2018-10-09 | 武汉科前生物股份有限公司 | 传染性法氏囊病毒病毒样颗粒的制备及其应用 |
| WO2023062182A1 (en) * | 2021-10-15 | 2023-04-20 | Aquilón Cyl, S.L. | Vaccine compositions against bovine viral diarrhea virus |
| CN114058524A (zh) * | 2021-10-28 | 2022-02-18 | 复旦大学 | 一种法氏囊亚病毒颗粒疫苗及其制备方法 |
Family Cites Families (65)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0174444B1 (en) | 1984-06-18 | 1992-03-11 | Chiron Corporation | Hepatitis surface antigen particle vaccine |
| US5614409A (en) | 1989-05-30 | 1997-03-25 | Commonwealth Scientific And Industrial Research Organisation | Production of IBDV VP2 in highly immunogenic form |
| US5641490A (en) | 1991-03-07 | 1997-06-24 | Virogenetics Corporation | Infectious bursal disease virus recombinant poxvirus vaccine |
| WO1992022575A1 (en) | 1991-06-19 | 1992-12-23 | Sri International | M-protein peptides of influenza virus as antiviral agents |
| JPH06509235A (ja) | 1991-07-26 | 1994-10-20 | ヴァイロジェネティクス コーポレイション | 伝染性ファブリキウス嚢病ウイルス組換えポックスウイルスワクチン |
| US5658572A (en) | 1991-07-26 | 1997-08-19 | Virogenetics Corporation | Infectious bursal disease virus recombinant poxvirus vaccine |
| US5290686A (en) | 1991-07-31 | 1994-03-01 | The United States Of America As Represented By The Department Of Health And Human Services | Expression of influenza a M2 protein in baculovirus |
| JPH05194597A (ja) | 1991-12-20 | 1993-08-03 | Nippon Seibutsu Kagaku Kenkyusho | 防御抗原性を有する伝染性ファブリキウス嚢病ウイルス（ｉｂｄｖ）の粒子製造法 |
| US5605792A (en) | 1993-11-04 | 1997-02-25 | The Ohio State University Research Foundation | Infectious bursal disease virus VP2 fusion protein expressed by baculovirus, use as diagnostic |
| US5788970A (en) | 1994-03-29 | 1998-08-04 | The University Of Maryland College Park | Chimeric infectious bursal disease virus CDNA clones, expression products and vaccines based thereon |
| AT402898B (de) | 1994-08-08 | 1997-09-25 | Int Centre Genetic Eng & Bio | Molekulares präsentiersystem |
| ES2282998T3 (es) | 1996-01-02 | 2007-10-16 | Institut Pasteur | Pseudo-particularmente viricas recombinadas y aplicaciomnes vacunales y antitumorales. |
| US5871744A (en) | 1996-09-05 | 1999-02-16 | University Of Maryland-Biotechnology Inst. | Method for generating birnavirus from synthetic RNA transcripts |
| US5916879A (en) | 1996-11-12 | 1999-06-29 | St. Jude Children's Research Hospital | DNA transcription unit vaccines that protect against avian influenza viruses and methods of use thereof |
| EP0861665A1 (en) | 1997-02-03 | 1998-09-02 | Dimminaco Ag/Sa/Ltd. | Infectious bursitis vaccine |
| AU750702B2 (en) | 1997-05-01 | 2002-07-25 | Chiron Corporation | Use of virus-like particles as adjuvants |
| CA2238659C (en) | 1997-05-26 | 2010-12-14 | Akzo Nobel N.V. | Recombinant birnavirus vaccine |
| EP0887412B1 (en) | 1997-05-26 | 2003-10-15 | Akzo Nobel N.V. | Recombinant birnavirus vaccine |
| US6596280B1 (en) | 1997-07-31 | 2003-07-22 | University Of Maryland Biotechnology Institute | Method for generating birnavirus from synthetic RNA transcripts |
| US6169175B1 (en) | 1997-08-06 | 2001-01-02 | Centers For Disease Control And Prevention | Preparation and use of recombinant influenza A virus M2 construct vaccines |
| US6231868B1 (en) | 1997-09-30 | 2001-05-15 | University Of Maryland-Biotechnology Institute | Method for generating nonpathogenic infections birnavirus from synthetic RNA transcripts |
| US6171591B1 (en) | 1997-12-08 | 2001-01-09 | Pentamer Pharmaceuticals, Inc. | Recombinant nodavirus compositions and methods |
| US6406843B1 (en) | 1998-02-27 | 2002-06-18 | University Of Arkansas | Poultry virus isolates and method |
| WO1999050419A2 (en) | 1998-03-31 | 1999-10-07 | University Of Maryland Biotechnology Institute | A method for generating nonpathogenic, infectious pancreatic necrosis virus (ipnv) from synthetic rna transcripts |
| US6114112A (en) | 1998-05-21 | 2000-09-05 | The Ohio State University | Method of making immunogenic compositions for infectious bursal disease virus |
| AU770802B2 (en) * | 1998-10-21 | 2004-03-04 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Virus-like particles for the induction of autoantibodies |
| HUP9802974A1 (hu) | 1998-12-19 | 2000-09-28 | Mezőgazdasági Biotechnológiai Kutatóközpont | Fertőző bursitis elleni DNS-vakcina |
| EP1980617A1 (en) | 1998-12-31 | 2008-10-15 | Novartis Vaccines and Diagnostics, Inc. | Improved expression of HIV polypeptides and production of virus-like particles |
| US6528063B2 (en) * | 1999-01-04 | 2003-03-04 | Rahan Meristem | Recombinant vaccines against IBDV |
| EP1069187A1 (en) | 1999-07-14 | 2001-01-17 | Stichting Dienst Landbouwkundig Onderzoek | Mosaic Infectious Bursal Disease Virus vaccines |
| FR2806087B1 (fr) | 2000-03-07 | 2002-10-18 | Agronomique Inst Nat Rech | Particules pseudovirales de rotavirus et leur utilisation pour vectoriser des proteines ou des acides nucleiques |
| AU5533601A (en) | 2000-04-14 | 2001-10-30 | Wisconsin Alumni Res Found | Viruses comprising mutation ion channel protein |
| US20020165176A1 (en) | 2000-05-01 | 2002-11-07 | Haynes Joel R. | Nucleic acid immunization |
| AU2001252458A1 (en) * | 2000-05-05 | 2001-11-20 | Martin Bachmann | Molecular antigen arrays and vaccines |
| WO2001097839A1 (en) | 2000-06-19 | 2001-12-27 | Rahan Meristem | Recombinant vaccines against infectious bursal disease virus |
| EP1294892B1 (en) | 2000-06-23 | 2007-10-17 | Wyeth Holdings Corporation | Assembly of wild-type and chimeric influenza virus-like particles (vlps) |
| AU2002211999A1 (en) * | 2000-10-02 | 2002-04-15 | Id Biomedical Corporation | Haemophilus influenzae antigens and corresponding DNA fragments |
| ATE433491T1 (de) | 2001-01-18 | 2009-06-15 | Vlaams Interuniv Inst Biotech | Oligomerische komplexe von chimären proteinen mit verbessertem immunogenen potential |
| FR2824327B1 (fr) * | 2001-05-02 | 2003-07-11 | Agronomique Inst Nat Rech | Particules pseudovirales de birnavirus |
| WO2002096940A2 (en) | 2001-05-31 | 2002-12-05 | Id-Lelystad, Institut Voor Dierhouderij En Diergezondheid B.V. | Attenuated very virulent infectious bursal disease virus |
| US6936256B2 (en) | 2001-08-10 | 2005-08-30 | University Of Maryland Biotechnology Institute | Sub-unit vaccine for infectious pancreatic necrosis virus |
| AUPR721101A0 (en) * | 2001-08-23 | 2001-09-13 | University Of Queensland, The | Nucleic acid and polypeptide linked to breast cancer |
| ATE447967T1 (de) | 2001-09-14 | 2009-11-15 | Cytos Biotechnology Ag | Verpackung von immunstimulierendem cpg in virusähnlichen partikeln: herstellungsverfahren und verwendung |
| EP1425040A2 (en) | 2001-09-14 | 2004-06-09 | Cytos Biotechnology AG | In vivo activation of antigen presenting cells for enhancement of immune responses induced by virus like particles |
| US6764684B2 (en) | 2001-09-28 | 2004-07-20 | Zeon Corporation | Avian herpesvirus-based recombinant infectious bursal disease vaccine |
| JP2005535287A (ja) * | 2002-02-11 | 2005-11-24 | アイディー バイオメディカル コーポレイション | グループｂ連鎖球菌抗原 |
| WO2003074552A1 (en) | 2002-03-01 | 2003-09-12 | Akzo Nobel N.V. | An infectious bursal disease virus variant |
| PT1506222E (pt) | 2002-05-17 | 2009-06-12 | Univ Cape Town | Proteínas l1 quiméricas do papilomavírus humano 16 incluindo um péptido l2, partículas semelhantes a vírus preparadas a partir daquelas e um método de preparação das partículas |
| ATE489969T1 (de) | 2002-06-20 | 2010-12-15 | Cytos Biotechnology Ag | Verpackte virusartige partikel in kombination mit cpg zur verwendung als adjuvantien mit allergenen. herstellungsverfahren und verwendung |
| CA2490866A1 (en) | 2002-06-26 | 2004-01-08 | Advanced Bionutrition Corporation | Viruses and virus-like particles for multiple antigen and target display |
| RU2324704C2 (ru) | 2002-07-17 | 2008-05-20 | Цитос Байотекнолоджи Аг | Молекулярные массивы антигенов |
| WO2004025263A2 (en) | 2002-09-16 | 2004-03-25 | Advanced Bionutrition Corporation | Protein and peptide expression for passive immunity |
| MXPA05009580A (es) | 2003-03-07 | 2005-10-19 | Merck & Co Inc | Vacuna contra el virus de la influenza. |
| ES2217967B1 (es) | 2003-03-31 | 2006-01-01 | Consejo Sup. Investig. Cientificas | Procedimiento de produccion de particulas virales vacias (vlps) del virus inductor de la bursitis infecciosa (ibdv), composiciones necesarias para su puesta a punto y su uso en la elaboracion de vacunas frente al ibdv. |
| JP5215561B2 (ja) | 2003-05-28 | 2013-06-19 | ウィスコンシン アルムニ リサーチ ファンデイション | ワクチン及び遺伝子治療のための高力価組換えインフルエンザ・ウイルス |
| CA2527102A1 (en) | 2003-07-10 | 2005-01-20 | Cytos Biotechnology Ag | Packaged virus-like particles |
| US8592197B2 (en) | 2003-07-11 | 2013-11-26 | Novavax, Inc. | Functional influenza virus-like particles (VLPs) |
| EP1689858B1 (en) | 2003-11-13 | 2013-05-15 | University Of Georgia Research Foundation, Inc. | Methods of characterizing infectious bursal disease virus |
| ES2307345B1 (es) | 2004-01-21 | 2009-11-13 | Consejo Sup. Investig. Cientificas | Capsidas vacias quimericas del virus causante de la enfermedad de la bursitis infecciosa (ibdv), su procedimiento de obtencion y aplicaciones. |
| ES2307346B1 (es) | 2004-01-21 | 2009-11-13 | Consejo Sup. Investig. Cientificas | Capsidas vacias (vlps(-vp4)) del virus causante de la enfermedad de la bursitis infecciosa (ibdv), su procedimiento de obtencion y aplicaciones. |
| ES2242542B1 (es) | 2004-04-30 | 2006-12-16 | Consejo Superior De Investigaciones Cientificas | Procedimiento para la produccion en levaduras de capsidas virales vacias compuestas por proteinas derivadas de pvp2 del virus causante de la enfermedad de la bursitis infecciosa (ibdv). |
| AU2005248361B2 (en) | 2004-05-18 | 2010-03-11 | Vical Incorporated | Influenza virus vaccine composition and methods of use |
| EP2578229B1 (en) | 2004-09-09 | 2013-07-10 | Novartis Vaccines and Diagnostics GmbH | Decreasing potential iatrogenic risks associated with vaccine antigens |
| BRPI0516953A (pt) | 2004-09-21 | 2008-09-30 | Cytos Biotechnology Ag | partìculas do tipo vìrus compreendendo uma proteìna de fusão da proteìna de revestimento de ap205 e um polipeptìdeo antigênico |
| US7244432B2 (en) | 2004-12-08 | 2007-07-17 | University Of Maryland Biotechnology Institute | Infectious bursal disease virus (IBDV) variant from Georgia |
-
2005
- 2005-07-15 ES ES200501733A patent/ES2310062B1/es not_active Expired - Fee Related
-
2006
- 2006-05-11 US US11/433,847 patent/US7476387B2/en active Active
- 2006-07-14 CA CA2615468A patent/CA2615468C/en active Active
- 2006-07-14 AU AU2006271978A patent/AU2006271978B2/en active Active
- 2006-07-14 JP JP2008520808A patent/JP5713533B2/ja not_active Expired - Fee Related
- 2006-07-14 ES ES06762595T patent/ES2371833T3/es active Active
- 2006-07-14 AT AT06762595T patent/ATE516345T1/de not_active IP Right Cessation
- 2006-07-14 CN CNA2006800342017A patent/CN101278043A/zh active Pending
- 2006-07-14 WO PCT/EP2006/006915 patent/WO2007009673A1/en not_active Ceased
- 2006-07-14 EP EP06762595A patent/EP1904626B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP1904626B1 (en) | 2011-07-13 |
| ES2371833T3 (es) | 2012-01-10 |
| US20070015243A1 (en) | 2007-01-18 |
| AU2006271978A1 (en) | 2007-01-25 |
| JP2009501012A (ja) | 2009-01-15 |
| US7476387B2 (en) | 2009-01-13 |
| ATE516345T1 (de) | 2011-07-15 |
| CN101278043A (zh) | 2008-10-01 |
| EP1904626A1 (en) | 2008-04-02 |
| WO2007009673A1 (en) | 2007-01-25 |
| CA2615468A1 (en) | 2007-01-25 |
| ES2310062B1 (es) | 2009-11-13 |
| ES2310062A1 (es) | 2008-12-16 |
| JP5713533B2 (ja) | 2015-05-07 |
| AU2006271978B2 (en) | 2012-08-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2615468C (en) | Chimeric empty viral-like particles derived from the infectious bursal disease virus (ibdv), process for their production and applications | |
| CN113416236B (zh) | 猪圆环病毒3型病毒样颗粒及其制备方法与应用 | |
| EP1706486B1 (en) | Chimeric empty capsids of the infectious bursal disease virus (ibdv), obtainment process and applications | |
| CN104693310A (zh) | 一种嵌合蛋白、病毒样颗粒及其应用 | |
| ES2242542B1 (es) | Procedimiento para la produccion en levaduras de capsidas virales vacias compuestas por proteinas derivadas de pvp2 del virus causante de la enfermedad de la bursitis infecciosa (ibdv). | |
| CN104628871A (zh) | 一种重组法氏囊病蛋白工程疫苗的制备 | |
| US6187319B1 (en) | Cross-protective rotavirus vaccine | |
| EP1706485B1 (en) | EMPTY CAPSIDS (VLPs(-VP4)) OF THE INFECTIOUS BURSAL DISEASE VIRUS (IBDV), OBTAINMENT PROCESS AND APPLICATIONS | |
| JP2022549257A (ja) | ファージｔ５由来ナノ粒子の作製及び機能化並びに治療用途 | |
| EP4495247A1 (en) | Virus-like particle containing capsid proteins connected by linker | |
| US20070010015A1 (en) | Complete empty viral particles of infectious bursal disease virus (ibdv), production method thereof and applications of same | |
| WO2025201387A1 (en) | A recombinant serotype 1 marek's disease virus | |
| US7132105B2 (en) | Icosahedral superstructure of VP2 of infectious bursitis virus (IBDV) and the application thereof | |
| CN120192991A (zh) | 一种通过大肠杆菌制备o型口蹄疫病毒多表位病毒样颗粒的方法及其产品和应用 | |
| TWI233947B (en) | Method for preparing IBDV infectious sub-unit and vaccine and diagnostic reagent made therefrom | |
| TW200525031A (en) | Method for producing virus-like particles of Infectious Bursal Disease viruses by E. coli and its application in subunit vaccine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |