CA2552790C - Process for the purification of bacterially expressed proteins - Google Patents

Process for the purification of bacterially expressed proteins Download PDF

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Publication number
CA2552790C
CA2552790C CA2552790A CA2552790A CA2552790C CA 2552790 C CA2552790 C CA 2552790C CA 2552790 A CA2552790 A CA 2552790A CA 2552790 A CA2552790 A CA 2552790A CA 2552790 C CA2552790 C CA 2552790C
Authority
CA
Canada
Prior art keywords
protein
buffer
solution
proteins
inclusion bodies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2552790A
Other languages
English (en)
French (fr)
Other versions
CA2552790A1 (en
Inventor
Mara Rossi
Luciano Capponi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ares Trading SA
Original Assignee
Ares Trading SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ares Trading SA filed Critical Ares Trading SA
Publication of CA2552790A1 publication Critical patent/CA2552790A1/en
Application granted granted Critical
Publication of CA2552790C publication Critical patent/CA2552790C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/521—Chemokines
    • C07K14/523—Beta-chemokines, e.g. RANTES, I-309/TCA-3, MIP-1alpha, MIP-1beta/ACT-2/LD78/SCIF, MCP-1/MCAF, MCP-2, MCP-3, LDCF-1, LDCF-2

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2552790A 2004-01-19 2004-01-19 Process for the purification of bacterially expressed proteins Expired - Fee Related CA2552790C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2004/050025 WO2005068500A1 (en) 2004-01-19 2004-01-19 Process for the purification of bacterially expressed proteins

Publications (2)

Publication Number Publication Date
CA2552790A1 CA2552790A1 (en) 2005-07-28
CA2552790C true CA2552790C (en) 2011-08-16

Family

ID=37055134

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2552790A Expired - Fee Related CA2552790C (en) 2004-01-19 2004-01-19 Process for the purification of bacterially expressed proteins

Country Status (14)

Country Link
EP (1) EP1439191B1 (es)
JP (1) JP4865569B2 (es)
AT (1) ATE322506T1 (es)
AU (1) AU2004313698B2 (es)
CA (1) CA2552790C (es)
CY (1) CY1105285T1 (es)
DE (1) DE60304435T2 (es)
DK (1) DK1439191T3 (es)
ES (1) ES2257642T3 (es)
IL (1) IL176763A (es)
NO (1) NO20063280L (es)
PT (1) PT1439191E (es)
SI (1) SI1439191T1 (es)
WO (1) WO2005068500A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1439191B1 (en) * 2004-01-19 2006-04-05 Ares Trading S.A. Process for the purification of bacterially expressed proteins
WO2005077973A1 (en) * 2004-02-11 2005-08-25 Virchow Biotech Private Limited Purification of recombinant human proteins
WO2010050485A1 (ja) * 2008-10-28 2010-05-06 学校法人関西学院 リフォールディング剤および蛋白質のリフォールディング方法
KR20240064367A (ko) * 2022-11-04 2024-05-13 주식회사 대웅제약 TGF-β3 단백질의 정제방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5912327A (en) * 1996-09-30 1999-06-15 Human Genome Sciences, Inc. Method of purifying chemokines from inclusion bodies
CA2336718A1 (en) * 1998-07-06 2000-01-13 Cambridge Neuroscience, Inc. Methods for treating neurological injuries and disorders
GB0006537D0 (en) * 2000-03-17 2000-05-10 Angeletti P Ist Richerche Bio Assays and screening methods
UA77950C2 (en) * 2000-10-04 2007-02-15 Applied Research Systems Use of mutants of cc chemokines for treatment of multiple sclerosis
IL162600A0 (en) * 2001-12-17 2005-11-20 Applied Research Systems Cc-chemokine mutants as cc-chemokine antagonists
SI21102A (sl) * 2001-12-19 2003-06-30 LEK, tovarna farmacevtskih in kemi�nih izdelkov, d.d. Postopek za izolacijo biološko aktivnega granulocitne kolonije stimulirajočega dejavnika
BR0312036A (pt) * 2002-06-25 2005-04-05 Pharmacia Corp ácido arilsulfonilhidroxâmico e derivados de amida bem como composição farmacêutica contendo os mesmos e uso dos mesmos
EP1439191B1 (en) * 2004-01-19 2006-04-05 Ares Trading S.A. Process for the purification of bacterially expressed proteins

Also Published As

Publication number Publication date
EP1439191B1 (en) 2006-04-05
IL176763A (en) 2011-02-28
IL176763A0 (en) 2006-10-31
DE60304435T2 (de) 2006-08-24
AU2004313698B2 (en) 2010-04-01
CY1105285T1 (el) 2010-03-03
ATE322506T1 (de) 2006-04-15
JP2007537150A (ja) 2007-12-20
CA2552790A1 (en) 2005-07-28
SI1439191T1 (sl) 2006-06-30
EP1439191A1 (en) 2004-07-21
PT1439191E (pt) 2006-06-30
ES2257642T3 (es) 2006-08-01
NO20063280L (no) 2006-10-16
JP4865569B2 (ja) 2012-02-01
WO2005068500A1 (en) 2005-07-28
AU2004313698A1 (en) 2005-07-28
DE60304435D1 (de) 2006-05-18
DK1439191T3 (da) 2006-08-14

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EEER Examination request
MKLA Lapsed

Effective date: 20140121