CA2495881C - Evolving new molecular function - Google Patents

Evolving new molecular function Download PDF

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Publication number
CA2495881C
CA2495881C CA2495881A CA2495881A CA2495881C CA 2495881 C CA2495881 C CA 2495881C CA 2495881 A CA2495881 A CA 2495881A CA 2495881 A CA2495881 A CA 2495881A CA 2495881 C CA2495881 C CA 2495881C
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Canada
Prior art keywords
codon
template
reactions
reactive
reaction
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Expired - Fee Related
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CA2495881A
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English (en)
French (fr)
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CA2495881A1 (en
Inventor
David R. Liu
Zev J. Gartner
Jeffrey B. Doyon
Christopher T. Calderone
Matthew W. Kanan
Xiaoyu Li
Thomas M. Snyder
Daniel M. Rosenbaum
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Harvard University
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Harvard University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
CA2495881A 2002-08-19 2003-08-19 Evolving new molecular function Expired - Fee Related CA2495881C (en)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US40439502P 2002-08-19 2002-08-19
US60/404,395 2002-08-19
US41966702P 2002-10-18 2002-10-18
US60/419,667 2002-10-18
US43281202P 2002-12-11 2002-12-11
US60/432,812 2002-12-11
US44477003P 2003-02-04 2003-02-04
US60/444,770 2003-02-04
US45778903P 2003-03-26 2003-03-26
US60/457,789 2003-03-26
US46986603P 2003-05-12 2003-05-12
US60/469,866 2003-05-12
US47949403P 2003-06-18 2003-06-18
US60/479,494 2003-06-18
PCT/US2003/025984 WO2004016767A2 (en) 2002-08-19 2003-08-19 Evolving new molecular function

Publications (2)

Publication Number Publication Date
CA2495881A1 CA2495881A1 (en) 2004-02-29
CA2495881C true CA2495881C (en) 2014-07-08

Family

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Family Applications (1)

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CA2495881A Expired - Fee Related CA2495881C (en) 2002-08-19 2003-08-19 Evolving new molecular function

Country Status (6)

Country Link
US (3) US7491494B2 (https=)
EP (1) EP1540013B1 (https=)
JP (2) JP4657919B2 (https=)
AU (2) AU2003263937B2 (https=)
CA (1) CA2495881C (https=)
WO (1) WO2004016767A2 (https=)

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