CA2471116A1 - Method for cloning of variable domain sequences - Google Patents
Method for cloning of variable domain sequences Download PDFInfo
- Publication number
- CA2471116A1 CA2471116A1 CA002471116A CA2471116A CA2471116A1 CA 2471116 A1 CA2471116 A1 CA 2471116A1 CA 002471116 A CA002471116 A CA 002471116A CA 2471116 A CA2471116 A CA 2471116A CA 2471116 A1 CA2471116 A1 CA 2471116A1
- Authority
- CA
- Canada
- Prior art keywords
- igvd
- primer
- sequences
- polynucleotide sequences
- variable domain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 claims abstract description 90
- 238000010367 cloning Methods 0.000 claims abstract description 28
- 108060003951 Immunoglobulin Proteins 0.000 claims abstract description 21
- 102000018358 immunoglobulin Human genes 0.000 claims abstract description 21
- 102000040430 polynucleotide Human genes 0.000 claims description 47
- 108091033319 polynucleotide Proteins 0.000 claims description 47
- 239000002157 polynucleotide Substances 0.000 claims description 47
- 239000012634 fragment Substances 0.000 claims description 45
- 108020004414 DNA Proteins 0.000 claims description 32
- 108020004999 messenger RNA Proteins 0.000 claims description 32
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 28
- 230000003321 amplification Effects 0.000 claims description 26
- 108091008146 restriction endonucleases Proteins 0.000 claims description 24
- 102000053602 DNA Human genes 0.000 claims description 20
- 239000013598 vector Substances 0.000 claims description 18
- 238000010804 cDNA synthesis Methods 0.000 claims description 12
- 230000000692 anti-sense effect Effects 0.000 claims description 10
- 238000003776 cleavage reaction Methods 0.000 claims description 8
- 230000007017 scission Effects 0.000 claims description 8
- 239000013604 expression vector Substances 0.000 claims description 6
- 210000004698 lymphocyte Anatomy 0.000 claims description 6
- 238000003556 assay Methods 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 229920001184 polypeptide Polymers 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
- 230000006820 DNA synthesis Effects 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 229940072221 immunoglobulins Drugs 0.000 abstract description 7
- 239000013615 primer Substances 0.000 description 140
- 241000894007 species Species 0.000 description 36
- 239000002299 complementary DNA Substances 0.000 description 32
- 108090000623 proteins and genes Proteins 0.000 description 25
- 210000004027 cell Anatomy 0.000 description 24
- 239000000427 antigen Substances 0.000 description 23
- 102000036639 antigens Human genes 0.000 description 23
- 108091007433 antigens Proteins 0.000 description 23
- 239000000047 product Substances 0.000 description 15
- 230000027455 binding Effects 0.000 description 12
- 230000035772 mutation Effects 0.000 description 11
- 241000282842 Lama glama Species 0.000 description 10
- 238000000137 annealing Methods 0.000 description 10
- 239000000499 gel Substances 0.000 description 10
- 239000000203 mixture Substances 0.000 description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
- 238000011534 incubation Methods 0.000 description 8
- 239000011230 binding agent Substances 0.000 description 7
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 6
- 235000002198 Annona diversifolia Nutrition 0.000 description 6
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- 238000002965 ELISA Methods 0.000 description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
- 238000000246 agarose gel electrophoresis Methods 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000000295 complement effect Effects 0.000 description 6
- 238000002649 immunization Methods 0.000 description 6
- 241000282836 Camelus dromedarius Species 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 229960000723 ampicillin Drugs 0.000 description 5
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 5
- 210000003719 b-lymphocyte Anatomy 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 230000003053 immunization Effects 0.000 description 5
- 238000002955 isolation Methods 0.000 description 5
- 102000039446 nucleic acids Human genes 0.000 description 5
- 108020004707 nucleic acids Proteins 0.000 description 5
- 150000007523 nucleic acids Chemical class 0.000 description 5
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 description 4
- 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 108091027305 Heteroduplex Proteins 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- 108010047041 Complementarity Determining Regions Proteins 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 102000004889 Interleukin-6 Human genes 0.000 description 3
- 108090001005 Interleukin-6 Proteins 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 238000012181 QIAquick gel extraction kit Methods 0.000 description 3
- 108091081021 Sense strand Proteins 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 239000002671 adjuvant Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 229940100601 interleukin-6 Drugs 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 239000002987 primer (paints) Substances 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 239000001226 triphosphate Substances 0.000 description 3
- 235000011178 triphosphate Nutrition 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 2
- 208000023275 Autoimmune disease Diseases 0.000 description 2
- 101710132601 Capsid protein Proteins 0.000 description 2
- 101710094648 Coat protein Proteins 0.000 description 2
- 101100109110 Danio rerio aph1b gene Proteins 0.000 description 2
- 235000017274 Diospyros sandwicensis Nutrition 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 2
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- 102100029768 Histone-lysine N-methyltransferase SETD1A Human genes 0.000 description 2
- 101000865038 Homo sapiens Histone-lysine N-methyltransferase SETD1A Proteins 0.000 description 2
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 description 2
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 description 2
- 102100034343 Integrase Human genes 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 101710125418 Major capsid protein Proteins 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 101710141454 Nucleoprotein Proteins 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 101710083689 Probable capsid protein Proteins 0.000 description 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical group 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 238000012869 ethanol precipitation Methods 0.000 description 2
- 102000037865 fusion proteins Human genes 0.000 description 2
- 108020001507 fusion proteins Proteins 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 230000028996 humoral immune response Effects 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 238000007857 nested PCR Methods 0.000 description 2
- 230000003472 neutralizing effect Effects 0.000 description 2
- -1 nucleotide triphosphates Chemical class 0.000 description 2
- 238000004091 panning Methods 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 230000037452 priming Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000006152 selective media Substances 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 108700026220 vif Genes Proteins 0.000 description 2
- 108010047303 von Willebrand Factor Proteins 0.000 description 2
- 102100036537 von Willebrand factor Human genes 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 238000012232 AGPC extraction Methods 0.000 description 1
- 208000003343 Antiphospholipid Syndrome Diseases 0.000 description 1
- 101100042371 Caenorhabditis elegans set-3 gene Proteins 0.000 description 1
- 101100421296 Caenorhabditis elegans set-6 gene Proteins 0.000 description 1
- 241000282826 Camelus Species 0.000 description 1
- 241000282828 Camelus bactrianus Species 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 241001524679 Escherichia virus M13 Species 0.000 description 1
- 241000724791 Filamentous phage Species 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 108010093488 His-His-His-His-His-His Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 1
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical group OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
- 241000577979 Peromyscus spicilegus Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 241000710078 Potyvirus Species 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 101150117538 Set2 gene Proteins 0.000 description 1
- 108010003723 Single-Domain Antibodies Proteins 0.000 description 1
- 108700012920 TNF Proteins 0.000 description 1
- 240000007591 Tilia tomentosa Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 101150117115 V gene Proteins 0.000 description 1
- 206010047115 Vasculitis Diseases 0.000 description 1
- 241000282840 Vicugna vicugna Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009824 affinity maturation Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000002494 anti-cea effect Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000002798 bone marrow cell Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 238000011962 continuous elektro deionisation Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000002532 enzyme inhibitor Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012997 ficoll-paque Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-L glutamate group Chemical group N[C@@H](CCC(=O)[O-])C(=O)[O-] WHUUTDBJXJRKMK-VKHMYHEASA-L 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000012933 kinetic analysis Methods 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 210000004180 plasmocyte Anatomy 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 206010039073 rheumatoid arthritis Diseases 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 201000000596 systemic lupus erythematosus Diseases 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Immunology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Pulmonology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01205100 | 2001-12-21 | ||
| EP01205100.9 | 2001-12-21 | ||
| PCT/EP2002/014662 WO2003054016A2 (en) | 2001-12-21 | 2002-12-20 | Method for cloning of variable domain sequences |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2471116A1 true CA2471116A1 (en) | 2003-07-03 |
Family
ID=8181517
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002471116A Abandoned CA2471116A1 (en) | 2001-12-21 | 2002-12-20 | Method for cloning of variable domain sequences |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20050037358A1 (enExample) |
| EP (1) | EP1456237A2 (enExample) |
| JP (2) | JP4323317B2 (enExample) |
| AU (1) | AU2002360068B2 (enExample) |
| CA (1) | CA2471116A1 (enExample) |
| NO (1) | NO20043066L (enExample) |
| WO (1) | WO2003054016A2 (enExample) |
Families Citing this family (170)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1558647B1 (en) | 2002-11-08 | 2015-06-10 | Ablynx N.V. | Single domain antibodies directed against tumour necrosis factor-alpha and uses therefor |
| WO2005044858A1 (en) | 2003-11-07 | 2005-05-19 | Ablynx N.V. | Camelidae single domain antibodies vhh directed against epidermal growth factor receptor and uses therefor |
| EP3299393A1 (en) | 2002-11-08 | 2018-03-28 | Ablynx N.V. | Single domain antibodies directed against tumour necrosis factor-alpha and uses therefor |
| GB0507044D0 (en) * | 2005-04-06 | 2005-05-11 | Celltech R&D Ltd | Primers |
| GB0507046D0 (en) * | 2005-04-06 | 2005-05-11 | Celltech R&D Ltd | Primers |
| CN103254309B (zh) | 2005-05-18 | 2017-09-26 | 埃博灵克斯股份有限公司 | 针对肿瘤坏死因子α的改进的纳米体TM |
| CN101213214B (zh) | 2005-05-20 | 2014-06-25 | 埃博灵克斯股份有限公司 | 针对冯威勒布兰特因子的单一结构域vhh抗体 |
| EP1999147A1 (en) * | 2006-03-27 | 2008-12-10 | Ablynx N.V. | Medical delivery device for therapeutic proteins based on single domain antibodies |
| CA2660592C (en) * | 2006-05-26 | 2016-07-12 | Macrogenics, Inc. | Humanized fc.gamma.riib-specific antibodies and methods of use thereof |
| CA2657763C (en) * | 2006-08-03 | 2016-05-31 | Vaccinex Inc. | Anti-il-6 monoclonal antibodies and uses thereof |
| EP3028716B1 (en) | 2006-10-10 | 2020-09-16 | Regenesance B.V. | Complement inhibition for improved nerve regeneration |
| EP2514767A1 (en) | 2006-12-19 | 2012-10-24 | Ablynx N.V. | Amino acid sequences directed against a metalloproteinase from the ADAM family and polypeptides comprising the same for the treatment of ADAM-related diseases and disorders |
| EP2115004A2 (en) | 2006-12-19 | 2009-11-11 | Ablynx N.V. | Amino acid sequences directed against gpcrs and polypeptides comprising the same for the treatment of gpcr-related diseases and disorders |
| DK2308514T3 (da) | 2007-03-23 | 2013-09-02 | To Bbb Holding B V | Konjugater til målrettet lægemiddeltransport gennem blod-hjerne barrieren |
| JP5240870B2 (ja) | 2007-07-03 | 2013-07-17 | アブリンクス エン.ヴェー. | 改善した免疫グロブリン配列を提供する方法 |
| CN101970490A (zh) | 2007-11-27 | 2011-02-09 | 埃博灵克斯股份有限公司 | 针对异二聚体细胞因子和/或其受体的氨基酸序列以及包括所述氨基酸序列的多肽 |
| FR2924440B1 (fr) * | 2007-12-04 | 2015-01-09 | Pf Medicament | Nouveau procede de generation et de criblage d'une banque d'anticorps |
| EP2088432A1 (en) * | 2008-02-11 | 2009-08-12 | MorphoSys AG | Methods for identification of an antibody or a target |
| CA2717015A1 (en) | 2008-03-05 | 2009-09-11 | Ablynx Nv | Novel antigen binding dimer-complexes, methods of making and uses thereof |
| WO2009124931A2 (en) | 2008-04-07 | 2009-10-15 | Ablynx Nv | Amino acid sequences directed against the notch pathways and uses thereof |
| WO2009127691A1 (en) | 2008-04-17 | 2009-10-22 | Ablynx N.V. | Peptides capable of binding to serum proteins and compounds, constructs and polypeptides comprising the same |
| US9212226B2 (en) | 2008-05-16 | 2015-12-15 | Ablynx N.V. | Amino acid sequences directed against CXCR4 and other GPCRs and compounds comprising the same |
| DK2285408T3 (en) | 2008-06-05 | 2019-02-04 | Ablynx Nv | AMINO ACID SEQUENCES AGAINST COATING PROTEINS IN A VIRUS AND POLYPEPTIDES INCLUDING THESE FOR TREATMENT OF VIRUSAL DISEASES |
| EP2313436B1 (en) | 2008-07-22 | 2014-11-26 | Ablynx N.V. | Amino acid sequences directed against multitarget scavenger receptors and polypeptides |
| CA2747644C (en) | 2008-12-19 | 2023-01-24 | Ablynx N.V. | Method for generation of immunoglobulin sequences |
| US10005830B2 (en) | 2009-03-05 | 2018-06-26 | Ablynx N.V. | Antigen binding dimer-complexes, methods of making/avoiding and uses thereof |
| DK2982690T3 (da) | 2009-04-30 | 2021-04-12 | Ablynx Nv | Fremgangsmåde til fremstilling af domæne-antistoffer |
| NZ596603A (en) | 2009-05-29 | 2013-07-26 | Morphosys Ag | A collection of antibodies or fragments having properties relevant to developability |
| HUE051430T2 (hu) | 2009-07-10 | 2021-03-01 | Ablynx Nv | Eljárás variábilis domének elõállítására |
| PT2473528E (pt) | 2009-09-03 | 2015-03-04 | Ablynx NV | Formulações estáveis de polipéptidos e seus usos |
| WO2011067160A1 (en) | 2009-12-01 | 2011-06-09 | Ablynx Nv | Von willebrand factor specific binding agents and uses thereof |
| US8962807B2 (en) | 2009-12-14 | 2015-02-24 | Ablynx N.V. | Single variable domain antibodies against OX40L, constructs and therapeutic use |
| WO2011083140A1 (en) | 2010-01-08 | 2011-07-14 | Ablynx Nv | Immunoglobulin single variable domain directed against human cxcr4 |
| WO2011095545A1 (en) | 2010-02-05 | 2011-08-11 | Ablynx Nv | Peptides capable of binding to serum albumin and compounds, constructs and polypeptides comprising the same |
| US9120855B2 (en) | 2010-02-10 | 2015-09-01 | Novartis Ag | Biologic compounds directed against death receptor 5 |
| HRP20191071T1 (hr) | 2010-02-11 | 2019-09-20 | Ablynx N.V. | Postupci i pripravci za pripremu aerosola |
| EP2533814A2 (en) | 2010-02-11 | 2012-12-19 | Ablynx N.V. | Delivery of immunoglobulin variable domains and constructs thereof |
| US9101674B2 (en) | 2010-03-29 | 2015-08-11 | Vib Vzw | Targeting and in vivo imaging of tumor-associated macrophages |
| US9556273B2 (en) | 2010-03-29 | 2017-01-31 | Vib Vzw | Anti-macrophage mannose receptor single variable domains for targeting and in vivo imaging of tumor-associated macrophages |
| SG185354A1 (en) | 2010-05-20 | 2012-12-28 | Ablynx Nv | Biological materials related to her3 |
| WO2011161263A1 (en) | 2010-06-25 | 2011-12-29 | Ablynx Nv | Pharmaceutical compositions for cutaneous administration |
| EP3279214B1 (en) | 2010-10-29 | 2024-10-09 | Ablynx NV | Method for the production of immunoglobulin single variable domains |
| DK2640742T3 (en) | 2010-11-19 | 2018-12-03 | Morphosys Ag | COLLECTION OF ANTIBODY SEQUENCES AND THEIR APPLICATIONS |
| EP2691415B1 (en) | 2011-03-28 | 2018-07-11 | Ablynx N.V. | Method for producing solid formulations comprising immunoglobulin single variable domains |
| UA117218C2 (uk) | 2011-05-05 | 2018-07-10 | Мерк Патент Гмбх | Поліпептид, спрямований проти il-17a, il-17f та/або il17-a/f |
| EP3590950A1 (en) | 2011-05-09 | 2020-01-08 | Ablynx NV | Method for the production of immunoglobulin single varible domains |
| RU2661677C2 (ru) | 2011-05-27 | 2018-07-18 | Аблинкс Нв | Ингибирование резорбции кости с помощью связывающих rank-l пептидов |
| US9580480B2 (en) | 2011-05-31 | 2017-02-28 | Massachusetts Institute Of Technology | Cell-directed synthesis of multifunctional nanopatterns and nanomaterials |
| JP2014525736A (ja) | 2011-06-23 | 2014-10-02 | アブリンクス エン.ヴェー. | IgEに対する免疫グロブリン単一可変ドメイン |
| WO2013041722A1 (en) | 2011-09-23 | 2013-03-28 | Ablynx Nv | Prolonged inhibition of interleukin-6 mediated signaling |
| CA2874309C (en) | 2012-05-24 | 2021-06-15 | Vib Vzw | Anti-macrophage mannose receptor single variable domains for targeting and in vivo imaging of tumor-associated macrophages |
| US11339208B1 (en) | 2012-05-31 | 2022-05-24 | United States Of America As Represented By The Secretary Of The Air Force | Camelidae single-domain antibodies against Yersinia pestis and methods of use |
| WO2014087010A1 (en) | 2012-12-07 | 2014-06-12 | Ablynx N.V. | IMPROVED POLYPEPTIDES DIRECTED AGAINST IgE |
| CN105102478A (zh) | 2013-01-30 | 2015-11-25 | 弗拉芒区生物技术研究所 | 用于筛选和药物发现目的的新型嵌合多肽 |
| EP3590578A1 (en) | 2013-02-05 | 2020-01-08 | VIB vzw | Muscarinic acetylcholine receptor binding agents and uses thereof |
| CA2906259C (en) | 2013-03-15 | 2022-12-06 | Vib Vzw | Anti-mmr single variable domains for prognosis and monitoring of cardiovascular diseases |
| PL2992101T3 (pl) | 2013-04-29 | 2019-05-31 | Agrosavfe Nv | Kompozycje agrochemiczne zawierające przeciwciała wiążące się ze sfingolipidami |
| NL1040254C2 (en) | 2013-05-17 | 2014-11-24 | Ablynx Nv | Stable formulations of immunoglobulin single variable domains and uses thereof. |
| EP2883883A1 (en) | 2013-12-16 | 2015-06-17 | Cardio3 Biosciences S.A. | Therapeutic targets and agents useful in treating ischemia reperfusion injury |
| WO2015121092A1 (en) | 2014-01-30 | 2015-08-20 | Vib Vzw | Opioid receptor binding agents and uses thereof |
| NL2013661B1 (en) | 2014-10-21 | 2016-10-05 | Ablynx Nv | KV1.3 Binding immunoglobulins. |
| CA2955554C (en) | 2014-07-22 | 2022-07-05 | Vib Vzw | Methods to select for agents that stabilize protein complexes |
| WO2016016329A1 (en) | 2014-07-29 | 2016-02-04 | Vrije Universiteit Brussel | Radio-labelled antibody fragments for use in the prognosis, diagnosis of cancer as well as for the prediction of cancer therapy response |
| CN106573983B (zh) | 2014-07-29 | 2018-05-25 | 布鲁塞尔自由大学 | 用于在癌症的预防和/或治疗中使用的放射性标记的抗体片段 |
| US9938522B2 (en) | 2014-09-25 | 2018-04-10 | Genewiz, Inc. | High throughput sequencing of end regions of long linear DNAs |
| BR112017009330A2 (pt) | 2014-11-05 | 2017-12-19 | Agrosavfe N V | planta transgênica que compreende um polinucleotídeo que codifica um domínio variável de anticorpo de cadeia pesada |
| JP6862343B2 (ja) | 2014-12-19 | 2021-04-21 | アブリンクス エン.ヴェー. | システイン結合ナノボディダイマー |
| MX390949B (es) | 2015-07-17 | 2025-03-21 | Univ Brussel Vrije | Fragmentos de anticuerpos radiomarcados para uso en tratamiento de cancer. |
| CA3006398A1 (en) | 2015-11-27 | 2017-06-01 | Ablynx Nv | Polypeptides inhibiting cd40l |
| US11243214B2 (en) | 2016-04-22 | 2022-02-08 | Université Libre de Bruxelles | Biomarker expressed in pancreatic beta cells useful in imaging or targeting beta cells |
| WO2017182605A1 (en) | 2016-04-22 | 2017-10-26 | Université Libre de Bruxelles | A new biomarker expressed in pancreatic beta cells useful in imaging or targeting beta cells |
| WO2017191108A1 (en) | 2016-05-02 | 2017-11-09 | Ablynx Nv | Treatment of rsv infection |
| WO2018007442A1 (en) | 2016-07-06 | 2018-01-11 | Ablynx N.V. | Treatment of il-6r related diseases |
| WO2018029182A1 (en) | 2016-08-08 | 2018-02-15 | Ablynx N.V. | Il-6r single variable domain antibodies for treatment of il-6r related diseases |
| EP3512880A1 (en) | 2016-09-15 | 2019-07-24 | Ablynx NV | Immunoglobulin single variable domains directed against macrophage migration inhibitory factor |
| AU2017361846B2 (en) | 2016-11-16 | 2024-08-22 | Ablynx Nv | T cell recruiting polypeptides capable of binding CD123 and TCR alpha/beta |
| WO2018099968A1 (en) | 2016-11-29 | 2018-06-07 | Ablynx N.V. | Treatment of infection by respiratory syncytial virus (rsv) |
| US11964002B2 (en) | 2017-02-28 | 2024-04-23 | Vib Vzw | Means and methods for oral protein delivery |
| US20200033347A1 (en) | 2017-04-18 | 2020-01-30 | Universite Libre De Bruxelles | Biomarkers And Targets For Proliferative Diseases |
| WO2018206734A1 (en) | 2017-05-11 | 2018-11-15 | Vib Vzw | Glycosylation of variable immunoglobulin domains |
| CN110997716B (zh) | 2017-06-02 | 2024-03-15 | 埃博灵克斯股份有限公司 | 结合聚集蛋白聚糖的免疫球蛋白 |
| IL270951B2 (en) | 2017-06-02 | 2025-05-01 | Merck Patent Gmbh | ADAMTS5, MMP13 and aggrecan-binding polypeptides |
| LT3630847T (lt) | 2017-06-02 | 2024-12-10 | Merck Patent Gmbh | Adamts surišantys imunoglobulinai |
| KR102778120B1 (ko) | 2017-06-02 | 2025-03-06 | 메르크 파텐트 게엠베하 | Mmp13 결합성 면역글로불린 |
| CA3070253A1 (en) | 2017-07-19 | 2019-01-24 | Vib Vzw | Serum albumin binding agents |
| WO2019086548A1 (en) | 2017-10-31 | 2019-05-09 | Vib Vzw | Novel antigen-binding chimeric proteins and methods and uses thereof |
| WO2019155041A1 (en) | 2018-02-12 | 2019-08-15 | Vib Vzw | Gβγ COMPLEX ANTIBODIES AND USES THEREOF |
| EP3758742A1 (en) | 2018-03-01 | 2021-01-06 | Vrije Universiteit Brussel | Human pd-l1-binding immunoglobulins |
| KR102879500B1 (ko) | 2018-03-23 | 2025-10-31 | 유니베르시테 리브레 드 브룩크젤즈 | Wnt 시그널링 작용제 분자 |
| CN112384236A (zh) | 2018-03-27 | 2021-02-19 | Umc乌得勒支控股有限公司 | 用于治疗微血管血栓形成的靶向溶栓 |
| SG11202111980QA (en) | 2019-04-29 | 2021-11-29 | Confo Therapeutics N V | Screening methods and assays for use with transmembrane proteins, in particular with gpcrs |
| EP3962599A1 (en) | 2019-04-30 | 2022-03-09 | Vib Vzw | Cystic fibrosis transmembrane conductance regulator stabilizing agents |
| WO2020239934A1 (en) | 2019-05-28 | 2020-12-03 | Vib Vzw | Cd8+ t-cells lacking plexins and their application in cancer treatment |
| US20220220197A1 (en) | 2019-05-28 | 2022-07-14 | Vib Vzw | Cancer Treatment by Targeting Plexins in the Immune Compartment |
| WO2021078786A1 (en) | 2019-10-21 | 2021-04-29 | Vib Vzw | Nanodisc-specific antigen-binding chimeric proteins |
| BR112022009087A2 (pt) | 2019-11-11 | 2022-07-26 | Ibi Ag Innovative Bio Insecticides Ltd | Nanocorpos para controle de insetos e usos dos mesmos |
| WO2021105438A1 (en) | 2019-11-27 | 2021-06-03 | Vib Vzw | Positive allosteric modulators of the calcium-sensing receptor |
| GB201918279D0 (en) | 2019-12-12 | 2020-01-29 | Vib Vzw | Glycosylated single chain immunoglobulin domains |
| EP4077372A1 (en) | 2019-12-20 | 2022-10-26 | Vib Vzw | Nanobody exchange chromatography |
| WO2021140205A1 (en) | 2020-01-10 | 2021-07-15 | Confo Therapeutics N.V. | Methods for generating antibodies and antibody fragments and libraries comprising same |
| WO2021156490A2 (en) | 2020-02-06 | 2021-08-12 | Vib Vzw | Corona virus binders |
| JP2023514654A (ja) | 2020-02-25 | 2023-04-06 | ブイアイビー ブイゼットダブリュ | ロイシンリッチリピートキナーゼ2のアロステリック調節因子 |
| AU2021250446A1 (en) | 2020-03-31 | 2022-11-17 | Biotalys NV | Anti-fungal polypeptides |
| US20230279115A1 (en) | 2020-04-22 | 2023-09-07 | Mabwell (shanghai) Bioscience Co., Ltd. | Single variable domain antibody targeting human programmed death ligand 1 (pd-l1) and derivative thereof |
| WO2021229104A1 (en) | 2020-05-15 | 2021-11-18 | Université de Liège | Anti-cd38 single-domain antibodies in disease monitoring and treatment |
| WO2022003156A1 (en) | 2020-07-02 | 2022-01-06 | Oncurious Nv | Ccr8 non-blocking binders |
| EP4189061A1 (en) | 2020-07-31 | 2023-06-07 | Biotalys NV | Methods of increasing recombinant protein yields |
| WO2022063947A1 (en) | 2020-09-24 | 2022-03-31 | Vib Vzw | Combination of p2y6 inhibitors and immune checkpoint inhibitors |
| WO2022063957A1 (en) | 2020-09-24 | 2022-03-31 | Vib Vzw | Biomarker for anti-tumor therapy |
| CA3195687A1 (en) | 2020-09-25 | 2022-03-31 | Ablynx Nv | Polypeptides comprising immunoglobulin single variable domains targeting il-13 and ox40l |
| JP2024508207A (ja) | 2020-12-02 | 2024-02-26 | ブイアイビー ブイゼットダブリュ | がんに対する組み合わせ治療におけるltbrアゴニスト |
| WO2022117569A1 (en) | 2020-12-02 | 2022-06-09 | Oncurious Nv | A ccr8 antagonist antibody in combination with a lymphotoxin beta receptor agonist antibody in therapy against cancer |
| KR20230123497A (ko) | 2020-12-18 | 2023-08-23 | 아블린쓰 엔.브이. | IL-6 및 TNF-α를 표적화하는 면역글로불린 단일 가변도메인을 포함하는 폴리펩티드 |
| GB202020502D0 (en) | 2020-12-23 | 2021-02-03 | Vib Vzw | Antibody composistion for treatment of corona virus infection |
| EP4267617A1 (en) | 2020-12-24 | 2023-11-01 | Vib Vzw | Human ccr8 binders |
| EP4267618A1 (en) | 2020-12-24 | 2023-11-01 | Vib Vzw | Non-blocking human ccr8 binders |
| WO2022136650A1 (en) | 2020-12-24 | 2022-06-30 | Oncurious Nv | Murine cross-reactive human ccr8 binders |
| CN117794566A (zh) | 2021-02-05 | 2024-03-29 | Vib研究所 | 沙贝病毒结合剂 |
| KR20230141853A (ko) | 2021-02-05 | 2023-10-10 | 브이아이비 브이지더블유 | 사베코바이러스 결합제 |
| CA3211257A1 (en) | 2021-02-17 | 2022-08-25 | Vib Vzw | Inhibition of slc4a4 in the treatment of cancer |
| CA3209052A1 (en) | 2021-02-19 | 2022-08-25 | Rafael Cristian CASELLAS | Single domain antibodies that neutralize sars-cov-2 |
| EP4294516A1 (en) | 2021-02-19 | 2023-12-27 | Vib Vzw | Cation-independent mannose-6-phosphate receptor binders |
| WO2022199804A1 (en) | 2021-03-24 | 2022-09-29 | Vib Vzw | Nek6 inhibition to treat als and ftd |
| WO2022242892A1 (en) | 2021-05-17 | 2022-11-24 | Université de Liège | Anti-cd38 single-domain antibodies in disease monitoring and treatment |
| WO2022268993A1 (en) | 2021-06-23 | 2022-12-29 | Vib Vzw | Means and methods for selection of specific binders |
| US20240343811A1 (en) | 2021-06-29 | 2024-10-17 | Shandong Simcere Biopharmacutical Co., Ltd. | Cd16 antibody and use thereof |
| EP4377352A2 (en) | 2021-07-30 | 2024-06-05 | Vib Vzw | Cation-independent mannose-6-phosphate receptor binders for targeted protein degradation |
| CA3227972A1 (en) | 2021-07-30 | 2023-02-02 | Shandong Simcere Biopharmaceutical Co., Ltd. | Anti-pvrig/anti-tigit bispecific antibodies and applications thereof |
| WO2023057601A1 (en) | 2021-10-06 | 2023-04-13 | Biotalys NV | Anti-fungal polypeptides |
| CN118696058A (zh) | 2021-12-03 | 2024-09-24 | 山东先声生物制药有限公司 | 抗bcma纳米抗体及其应用 |
| EP4448574A1 (en) | 2021-12-17 | 2024-10-23 | Ablynx N.V. | Polypeptides comprising immunoglobulin single variable domains targeting tcralphabeta, cd33 and cd123 |
| JP2025501206A (ja) | 2021-12-31 | 2025-01-17 | 山▲東▼先声生物制▲薬▼有限公司 | Gprc5d抗体及びその応用 |
| EP4463479A1 (en) | 2022-01-12 | 2024-11-20 | Vib Vzw | Human ntcp binders for therapeutic use and liver-specific targeted delivery |
| WO2023148291A1 (en) | 2022-02-02 | 2023-08-10 | Biotalys NV | Methods for genome editing |
| WO2023148397A1 (en) | 2022-02-07 | 2023-08-10 | Vib Vzw | Engineered stabilizing aglycosylated fc-regions |
| EP4508082A1 (en) | 2022-04-13 | 2025-02-19 | Vib Vzw | An ltbr agonist in combination therapy against cancer |
| WO2023213751A1 (en) | 2022-05-02 | 2023-11-09 | Umc Utrecht Holding B.V | Single domain antibodies for the detection of plasmin-cleaved vwf |
| US20250313611A1 (en) | 2022-05-18 | 2025-10-09 | Vib Vzw | Sarbecovirus spike s2 subunit binders |
| JP2025522351A (ja) | 2022-06-06 | 2025-07-15 | 山▲東▼先声生物制▲薬▼有限公司 | Bcma、gprc5d及びt細胞を標的とする多重特異性抗体並びにその応用 |
| JP2025523630A (ja) | 2022-07-04 | 2025-07-23 | ブイアイビー ブイゼットダブリュ | 血液-脳脊髄液関門通過抗体 |
| WO2024068744A1 (en) | 2022-09-27 | 2024-04-04 | Vib Vzw | Antivirals against human parainfluenza virus |
| WO2024083843A1 (en) | 2022-10-18 | 2024-04-25 | Confo Therapeutics N.V. | Amino acid sequences directed against the melanocortin 4 receptor and polypeptides comprising the same for the treatment of mc4r-related diseases and disorders |
| JP2025537809A (ja) | 2022-11-15 | 2025-11-20 | アイメック・ヴェーゼットウェー | 液滴操作のための方法およびシステム |
| EP4634214A1 (en) | 2022-12-15 | 2025-10-22 | Aarhus Universitet | Synthetic activation of multimeric transmembrane receptors |
| WO2024133937A1 (en) | 2022-12-22 | 2024-06-27 | Biotalys NV | Methods for genome editing |
| WO2024145551A1 (en) | 2022-12-29 | 2024-07-04 | Biotalys NV | Agrochemical compositions |
| EP4642233A1 (en) | 2022-12-30 | 2025-11-05 | Biotalys NV | Agglomerate |
| WO2024141641A2 (en) | 2022-12-30 | 2024-07-04 | Biotalys NV | Secretion signals |
| WO2024141638A1 (en) | 2022-12-30 | 2024-07-04 | Biotalys NV | Self-emulsifiable concentrate |
| EP4655323A1 (en) | 2023-01-27 | 2025-12-03 | Vrije Universiteit Brussel | Cd8b-binding polypeptides |
| WO2024156888A1 (en) | 2023-01-27 | 2024-08-02 | Vib Vzw | Cd163-binding conjugates |
| EP4662246A1 (en) | 2023-02-09 | 2025-12-17 | Seni-Preps B.V. | Immunoglobulin single variable domains that inhibit urease and use thereof |
| WO2024175787A1 (en) | 2023-02-24 | 2024-08-29 | Vrije Universiteit Brussel | Anti-inflammatory pannexin 1 channel inhibitors |
| US20240344078A1 (en) | 2023-03-14 | 2024-10-17 | Aarhus Universitet | Genetically altered nfr5 receptor kinases |
| AU2024243709A1 (en) | 2023-04-03 | 2025-11-06 | Katholieke Universiteit Leuven | Blood-brain barrier crossing antibodies |
| WO2024231348A1 (en) | 2023-05-11 | 2024-11-14 | Vib Vzw | Slc4a4/nbce1 inhibitors |
| WO2024240162A1 (en) | 2023-05-23 | 2024-11-28 | Shanghai Allygen Biologics Co., Ltd. | Pd-l1 and trop-2 targeting conjugates comprising effector molecules and uses thereof |
| WO2024261344A1 (en) | 2023-06-23 | 2024-12-26 | Vib Vzw | Novel binders targeting the multi-drug resistant pathogen acinetobacter baumannii |
| EP4483951A1 (en) | 2023-06-30 | 2025-01-01 | Université de Liège | Single-domain antibody for inhibition of neutrophil elastase activity |
| NL2036011B1 (en) | 2023-10-12 | 2025-04-30 | Synapse Res Institute | Molecules for reversing anti-coagulant activity of direct oral anticoagulants |
| WO2025093683A1 (en) | 2023-11-03 | 2025-05-08 | Neuvasq Biotechnologies Sa | Wnt7 signaling agonists |
| WO2025109176A1 (en) | 2023-11-22 | 2025-05-30 | Exevir Bio Bv | Optimized sarbecovirus spike s2 subunit binders and compositions comprising the same |
| WO2025125577A1 (en) | 2023-12-14 | 2025-06-19 | Vib Vzw | Antibodies against influenza b virus |
| EP4574981A1 (en) | 2023-12-22 | 2025-06-25 | Biotalys NV | Anti-fungal vhh antibodies |
| WO2025154058A1 (en) | 2024-01-21 | 2025-07-24 | Ibi-Ag Innovative Bio Insecticides Ltd. | Anti-insect hsp70 nanobodies and uses thereof |
| WO2025154056A1 (en) | 2024-01-21 | 2025-07-24 | Ibi-Ag Innovative Bio Insecticides Ltd. | Anti-insect cda nanobodies and uses thereof |
| WO2025181155A1 (en) | 2024-02-26 | 2025-09-04 | Vib Vzw | Human beta-glucocerebrosidase binders and uses thereof |
| WO2025196308A1 (en) | 2024-03-22 | 2025-09-25 | Vib Vzw | Means and methods for displaying fc-containing proteins on cells and selection thereof |
| WO2025219231A1 (en) | 2024-04-15 | 2025-10-23 | Vib Vzw | Computer-implemented means and methods for the de novo design of antibodies targeting a specific epitope |
| WO2025238157A1 (en) | 2024-05-15 | 2025-11-20 | Katholieke Universiteit Leuven | Multispecific binding agent suitable for use in cancer immune therapy |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990005144A1 (en) * | 1988-11-11 | 1990-05-17 | Medical Research Council | Single domain ligands, receptors comprising said ligands, methods for their production, and use of said ligands and receptors |
| US6291161B1 (en) * | 1989-05-16 | 2001-09-18 | Scripps Research Institute | Method for tapping the immunological repertiore |
| GB9015198D0 (en) * | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
| EP0592626B1 (en) * | 1992-03-11 | 2003-01-29 | Dana-Farber Cancer Institute, Inc. | METHODS TO CLONE mRNA |
| EP0656946B2 (en) * | 1992-08-21 | 2010-03-31 | Vrije Universiteit Brussel | Immunoglobulins devoid of light chains |
| US5580726A (en) * | 1994-04-29 | 1996-12-03 | Geron Corporation | Method and Kit for enhanced differential display |
| AU6808194A (en) * | 1993-05-31 | 1994-12-20 | Chugai Seiyaku Kabushiki Kaisha | Reconstructed human antibody against human interleukin-6 |
| WO1997002479A2 (en) * | 1995-06-30 | 1997-01-23 | Yale University | Human monoclonal anti-tumor antibodies |
| US6114119A (en) * | 1997-08-29 | 2000-09-05 | Wisconsin Alumni Research Foundation | Transglutaminase and gene encoding same |
| BR9813276A (pt) * | 1997-10-27 | 2000-08-22 | Unilever Nv | Proteìna multivalente de ligação de antìgeno, sequências de nucleotìdeos, vetor de expressão, célula hospedeira, processo para preparação de proteìna multivalente de ligação de antìgeno, e, uso da mesma |
| JP4213586B2 (ja) * | 2001-09-13 | 2009-01-21 | 株式会社抗体研究所 | ラクダ抗体ライブラリーの作製方法 |
| AU2002351896A1 (en) * | 2001-12-11 | 2003-06-23 | Ablynx N.V. | Method for displaying loops from immunoglobulin domains in different contexts |
-
2002
- 2002-12-20 AU AU2002360068A patent/AU2002360068B2/en not_active Expired - Fee Related
- 2002-12-20 JP JP2003554732A patent/JP4323317B2/ja not_active Expired - Fee Related
- 2002-12-20 WO PCT/EP2002/014662 patent/WO2003054016A2/en not_active Ceased
- 2002-12-20 EP EP02795255A patent/EP1456237A2/en not_active Withdrawn
- 2002-12-20 US US10/499,500 patent/US20050037358A1/en not_active Abandoned
- 2002-12-20 CA CA002471116A patent/CA2471116A1/en not_active Abandoned
-
2004
- 2004-07-19 NO NO20043066A patent/NO20043066L/no not_active Application Discontinuation
-
2006
- 2006-09-12 US US11/519,750 patent/US20070009527A1/en not_active Abandoned
-
2009
- 2009-03-13 JP JP2009060673A patent/JP2009171975A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2002360068B2 (en) | 2009-09-03 |
| AU2002360068A1 (en) | 2003-07-09 |
| NO20043066L (no) | 2004-09-21 |
| JP4323317B2 (ja) | 2009-09-02 |
| EP1456237A2 (en) | 2004-09-15 |
| US20050037358A1 (en) | 2005-02-17 |
| US20070009527A1 (en) | 2007-01-11 |
| WO2003054016A2 (en) | 2003-07-03 |
| WO2003054016A3 (en) | 2004-02-19 |
| WO2003054016A8 (en) | 2004-09-30 |
| JP2009171975A (ja) | 2009-08-06 |
| JP2005524391A (ja) | 2005-08-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2002360068B2 (en) | Method for cloning of variable domain sequences | |
| EP2281837B1 (en) | Antigen binding domains from fish | |
| JP3344584B2 (ja) | 組換えライブラリースクリーニング法 | |
| JP5997154B2 (ja) | 多重特異性多価抗体の生成方法 | |
| US20050214857A1 (en) | Method for displaying loops from immunoglobulin domains in different contexts | |
| JP2014527408A (ja) | 可溶性ポリペプチド | |
| WO2017197667A1 (zh) | 抗人pd-l1人源化单克隆抗体及其应用 | |
| JP2015524404A (ja) | 高度に保存された標的に対するラクダ科動物由来の配列を含む抗体 | |
| US20100069262A1 (en) | Method for Cloning Avian-Derived Antibodies | |
| US10336816B2 (en) | Phage-displayed single-chain variable fragment library | |
| JP2023518900A (ja) | 抗原特異的結合ポリペプチド遺伝子ディスプレイベクターの構築方法および用途 | |
| JP2012503983A (ja) | 適合性ディスプレイベクター系 | |
| KR102194203B1 (ko) | 항체 나이브 라이브러리의 생성 방법, 상기 라이브러리 및 그 적용(들) | |
| US12378697B2 (en) | Method for preparing phage library | |
| JP2012503982A (ja) | 適合性ディスプレイベクター系 | |
| Marks | V-Gene Repertoires Expressed on Bacteriophage | |
| US11001833B2 (en) | Method and kit for generating high affinity binding agents | |
| EP3209699A1 (en) | Vh scaffold | |
| GB2616707A (en) | Methods | |
| KR100341827B1 (ko) | 아미노-rna에 대한 항체 및 이의 제조방법 | |
| US9938522B2 (en) | High throughput sequencing of end regions of long linear DNAs | |
| HK1154026A (en) | Antigen binding domains from fish |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |