CA2466298A1 - Methods of increasing protein expression levels - Google Patents

Methods of increasing protein expression levels Download PDF

Info

Publication number
CA2466298A1
CA2466298A1 CA002466298A CA2466298A CA2466298A1 CA 2466298 A1 CA2466298 A1 CA 2466298A1 CA 002466298 A CA002466298 A CA 002466298A CA 2466298 A CA2466298 A CA 2466298A CA 2466298 A1 CA2466298 A1 CA 2466298A1
Authority
CA
Canada
Prior art keywords
tnfred
amino acid
proline
substituted
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002466298A
Other languages
English (en)
French (fr)
Inventor
William Brondyk
Xuliang Jiang
Rene Lynn Schweickhardt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Research Systems ARS Holding NV
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2466298A1 publication Critical patent/CA2466298A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7151Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00Peptide or protein sequence
    • Y10S930/01Peptide or protein sequence
    • Y10S930/14Lymphokine; related peptides
    • Y10S930/144Tumor necrosis factor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
CA002466298A 2001-11-30 2002-11-21 Methods of increasing protein expression levels Abandoned CA2466298A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34064801P 2001-11-30 2001-11-30
US60/340,648 2001-11-30
PCT/EP2002/013059 WO2003046160A2 (en) 2001-11-30 2002-11-21 Methods of increasing protein expression levels

Publications (1)

Publication Number Publication Date
CA2466298A1 true CA2466298A1 (en) 2003-06-05

Family

ID=23334343

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002466298A Abandoned CA2466298A1 (en) 2001-11-30 2002-11-21 Methods of increasing protein expression levels

Country Status (12)

Country Link
US (2) US7258997B2 (enExample)
EP (1) EP1448772B1 (enExample)
JP (1) JP2005535281A (enExample)
AT (1) ATE367438T1 (enExample)
AU (1) AU2002338947B2 (enExample)
CA (1) CA2466298A1 (enExample)
DE (1) DE60221273D1 (enExample)
DK (1) DK1448772T3 (enExample)
ES (1) ES2290337T3 (enExample)
IL (1) IL162133A0 (enExample)
PT (1) PT1448772E (enExample)
WO (1) WO2003046160A2 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7258997B2 (en) * 2001-11-30 2007-08-21 Applied Research Systems Ars Holding N.V. Methods of increasing protein expression levels
EP1697414A2 (en) * 2003-12-23 2006-09-06 Applied Research Systems ARS Holding N.V. Process for the production of tumor necrosis factor-binding proteins
US8722348B2 (en) 2008-05-28 2014-05-13 Wayne State University Method and composition for a protein transduction technology and its applications
EP3777538B1 (en) 2011-05-02 2023-06-28 Wayne State University A protein-induced pluripotent cell technology and uses thereof
PL3060237T3 (pl) 2013-10-25 2022-02-21 Wayne State University Zmodyfikowane białko przeprogramowujące do stosowania w leczeniu raka
EP3987028A4 (en) * 2019-06-21 2023-07-19 Kernal Biologics, Inc. MODIFIED ONCOSELECTIVE PROTEIN EXPRESSION
IL295259A (en) * 2020-02-03 2022-10-01 Premas Biotech Private Ltd Recombinant expression platform, constructs and methods for expression of proteins that are difficult to express (dte-ps)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2238070T3 (es) * 1989-04-21 2005-08-16 Amgen Inc. Receptor del tnf, proteina ligante del tnf y adn codante para estos.
AU3073692A (en) * 1991-11-25 1993-06-28 Richardson-Vicks Inc. Compositions for regulating skin wrinkles and/or skin atrophy
IL107268A0 (en) * 1993-10-12 1994-01-25 Yeda Res & Dev Molecules influencing the shedding of the tnf receptor, their preparation and their use
IL159015A0 (en) * 2001-05-25 2004-05-12 Genset Sa Polypeptides, their preparation and use
US7258997B2 (en) * 2001-11-30 2007-08-21 Applied Research Systems Ars Holding N.V. Methods of increasing protein expression levels

Also Published As

Publication number Publication date
PT1448772E (pt) 2007-10-02
WO2003046160A2 (en) 2003-06-05
ES2290337T3 (es) 2008-02-16
ATE367438T1 (de) 2007-08-15
DE60221273D1 (de) 2007-08-30
WO2003046160A3 (en) 2003-07-31
JP2005535281A (ja) 2005-11-24
EP1448772B1 (en) 2007-07-18
AU2002338947A1 (en) 2003-06-10
IL162133A0 (en) 2005-11-20
US20050074840A1 (en) 2005-04-07
US7258997B2 (en) 2007-08-21
US20080014635A1 (en) 2008-01-17
DK1448772T3 (da) 2007-11-19
EP1448772A2 (en) 2004-08-25
AU2002338947B2 (en) 2007-09-13

Similar Documents

Publication Publication Date Title
CN100400548C (zh) 具有增强的体内红细胞生成素活性的融合蛋白
Harris et al. Assessing genetic heterogeneity in production cell lines: detection by peptide mapping of a low level Tyr to Gln sequence variant in a recombinant antibody
CN112375784B (zh) 制备重组新型冠状病毒Spike蛋白的方法
US20080014635A1 (en) Methods of increasing protein expression levels
JP3946638B2 (ja) 生体内エリスロポエチン活性が増強された融合蛋白質
US8454969B2 (en) Soluble tumor necrosis factor receptor mutant
CA3237004A1 (en) B cell activating factor (baff)-a proliferation inducing ligand (april) dual inhibitors
US6555373B1 (en) Methods for identifying human cell lines useful for endogenous gene activation isolated human cell lines identified thereby, and uses thereof
EP1487479B1 (en) Mutant glycoproteins
CN112553256B (zh) Il-2受体复合物及其制备方法与应用
CN117701633A (zh) Cho细胞中调控抗体高甘露糖糖型的方法、cho细胞及其应用
CN113564185A (zh) 一种碱性磷酸酶基因及其cho稳定细胞株和alp制备方法
HK40041902B (en) Method for preparing a recombinant novel coronavirus spike protein
HK40041902A (en) Method for preparing a recombinant novel coronavirus spike protein
LT4012B (en) Process for the preparation of polipeptides
JPH03123487A (ja) 新規ポリペプチドの製造法
CA2415095A1 (en) Secreted alpha-helical protein-36
JPH0653758B2 (ja) 新規ジヒドロ葉酸還元酵素一成長ホルモン放出因子誘導体融合タンパク質
JP2011200147A (ja) Fcレセプターをコードするポリヌクレオチド、およびそれを用いたFcレセプターの製造方法
HK1101315A1 (en) Fsh glycosylation mutant

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued