CA2412882A1 - Polypeptides glycosyles a extension peptidique - Google Patents

Polypeptides glycosyles a extension peptidique Download PDF

Info

Publication number
CA2412882A1
CA2412882A1 CA002412882A CA2412882A CA2412882A1 CA 2412882 A1 CA2412882 A1 CA 2412882A1 CA 002412882 A CA002412882 A CA 002412882A CA 2412882 A CA2412882 A CA 2412882A CA 2412882 A1 CA2412882 A1 CA 2412882A1
Authority
CA
Canada
Prior art keywords
polypeptide
amino acid
peptide
thr
asn
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002412882A
Other languages
English (en)
Inventor
Jens Sigurd Okkels
Anne Dam Jensen
Bart Van Den Hazel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Maxygen ApS
Maxygen Holdings Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/DK2000/000743 external-priority patent/WO2001049830A2/fr
Priority claimed from PCT/DK2001/000090 external-priority patent/WO2001058493A1/fr
Application filed by Individual filed Critical Individual
Publication of CA2412882A1 publication Critical patent/CA2412882A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A61K47/646—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent the entire peptide or protein drug conjugate elicits an immune response, e.g. conjugate vaccines
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01003—Long-chain-fatty-acid-CoA ligase (6.2.1.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

L'invention concerne des polypeptides glycosyl~s comprenant la structure primaire NH2-X-Pp-COOH dans laquelle X est une addition peptidique contenant un site de glycosylation ou contribuant ~ ce dernier, et Pp est un polypeptide d'int~rÚt. La structure primaire peut Útre Nh2-Px-X-Py-COOH dans laquelle Px est une partie N-terminale d'un polypeptide Pp d'int~rÚt, Py est une partie C-terminale dudit polypeptide Pp, et X est une addition peptidique contenant un site de glycosylation ou contribuant ~ ce dernier. Les polypeptides glycosyl~s poss­dent des propri~t~s am~lior~es par rapport au polypeptide d'int~rÚt.
CA002412882A 2000-06-30 2001-06-29 Polypeptides glycosyles a extension peptidique Abandoned CA2412882A1 (fr)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
DKPA200001027 2000-06-30
DKPA200001027 2000-06-30
DKPA200001092 2000-07-14
DKPA200001092 2000-07-14
PCT/DK2000/000743 WO2001049830A2 (fr) 1999-12-30 2000-12-29 Enzymes lysosomales ameliorees et activateurs d"enzymes lysosomales
DKPCT/DK00/00743 2000-12-29
DKPCT/DK01/00090 2001-02-09
PCT/DK2001/000090 WO2001058493A1 (fr) 2000-02-11 2001-02-09 Conjugates of follicle stimulating hormones
PCT/DK2001/000459 WO2002002597A2 (fr) 2000-06-30 2001-06-29 Polypeptides glycosyles a extension peptidique

Publications (1)

Publication Number Publication Date
CA2412882A1 true CA2412882A1 (fr) 2002-01-10

Family

ID=56290160

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002412882A Abandoned CA2412882A1 (fr) 2000-06-30 2001-06-29 Polypeptides glycosyles a extension peptidique

Country Status (5)

Country Link
EP (1) EP1299535A2 (fr)
JP (1) JP2004504016A (fr)
AU (1) AU2001267337A1 (fr)
CA (1) CA2412882A1 (fr)
WO (1) WO2002002597A2 (fr)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7265084B2 (en) 2001-10-10 2007-09-04 Neose Technologies, Inc. Glycopegylation methods and proteins/peptides produced by the methods
ES2564688T3 (es) 2001-10-10 2016-03-28 Ratiopharm Gmbh Remodelación y glicoconjugación de poli(éter) a eritropoyetina
US7214660B2 (en) 2001-10-10 2007-05-08 Neose Technologies, Inc. Erythropoietin: remodeling and glycoconjugation of erythropoietin
US7226903B2 (en) 2001-10-10 2007-06-05 Neose Technologies, Inc. Interferon beta: remodeling and glycoconjugation of interferon beta
US7439043B2 (en) 2001-10-10 2008-10-21 Neose Technologies, Inc. Galactosyl nucleotide sugars
US7795210B2 (en) 2001-10-10 2010-09-14 Novo Nordisk A/S Protein remodeling methods and proteins/peptides produced by the methods
US7297511B2 (en) 2001-10-10 2007-11-20 Neose Technologies, Inc. Interferon alpha: remodeling and glycoconjugation of interferon alpha
US7179617B2 (en) 2001-10-10 2007-02-20 Neose Technologies, Inc. Factor IX: remolding and glycoconjugation of Factor IX
US7399613B2 (en) 2001-10-10 2008-07-15 Neose Technologies, Inc. Sialic acid nucleotide sugars
US7696163B2 (en) 2001-10-10 2010-04-13 Novo Nordisk A/S Erythropoietin: remodeling and glycoconjugation of erythropoietin
US8008252B2 (en) 2001-10-10 2011-08-30 Novo Nordisk A/S Factor VII: remodeling and glycoconjugation of Factor VII
US7125843B2 (en) 2001-10-19 2006-10-24 Neose Technologies, Inc. Glycoconjugates including more than one peptide
US7265085B2 (en) 2001-10-10 2007-09-04 Neose Technologies, Inc. Glycoconjugation methods and proteins/peptides produced by the methods
US7157277B2 (en) 2001-11-28 2007-01-02 Neose Technologies, Inc. Factor VIII remodeling and glycoconjugation of Factor VIII
US7173003B2 (en) 2001-10-10 2007-02-06 Neose Technologies, Inc. Granulocyte colony stimulating factor: remodeling and glycoconjugation of G-CSF
US7473680B2 (en) 2001-11-28 2009-01-06 Neose Technologies, Inc. Remodeling and glycoconjugation of peptides
US7776581B2 (en) 2002-06-17 2010-08-17 Chr. Hansen A/S Method of producing an aspartic protease in a recombinant host organism
US7553930B2 (en) 2003-01-06 2009-06-30 Xencor, Inc. BAFF variants and methods thereof
BRPI0408358A (pt) 2003-03-14 2006-03-21 Neose Technologies Inc polìmeros hidrossolúveis ramificados e seus conjugados
US8791070B2 (en) 2003-04-09 2014-07-29 Novo Nordisk A/S Glycopegylated factor IX
WO2006127896A2 (fr) 2005-05-25 2006-11-30 Neose Technologies, Inc. Facteur ix glycopegyle
US7691603B2 (en) 2003-04-09 2010-04-06 Novo Nordisk A/S Intracellular formation of peptide conjugates
EP2338333B1 (fr) 2003-04-09 2017-09-06 ratiopharm GmbH Méthode de glycopegylation et proteines/peptides produits au moyen de ces méthodes
ES2380093T3 (es) 2003-05-09 2012-05-08 Biogenerix Ag Composiciones y métodos para la preparación de mutantes de glucosilación de la hormona del crecimiento humana
US9005625B2 (en) 2003-07-25 2015-04-14 Novo Nordisk A/S Antibody toxin conjugates
US20080305992A1 (en) 2003-11-24 2008-12-11 Neose Technologies, Inc. Glycopegylated erythropoietin
JP4719686B2 (ja) 2003-11-24 2011-07-06 バイオジェネリックス アーゲー ＧｌｙｃｏＰＥＧ化エリスロポエチン
US8633157B2 (en) 2003-11-24 2014-01-21 Novo Nordisk A/S Glycopegylated erythropoietin
US7956032B2 (en) 2003-12-03 2011-06-07 Novo Nordisk A/S Glycopegylated granulocyte colony stimulating factor
US20060040856A1 (en) 2003-12-03 2006-02-23 Neose Technologies, Inc. Glycopegylated factor IX
AU2005206796B2 (en) 2004-01-08 2011-06-16 Ratiopharm Gmbh O-linked glycosylation of peptides
CA2560590C (fr) * 2004-03-25 2015-06-16 Iogen Bio-Products Corporation Xylanases modifiees a expression amelioree
MXPA06012676A (es) 2004-05-04 2007-04-02 Novo Nordisk Healthcare Ag Glucoformas o-enlazadas de polipeptidos y metodos de fabricacion de los mismos.
US20080300173A1 (en) 2004-07-13 2008-12-04 Defrees Shawn Branched Peg Remodeling and Glycosylation of Glucagon-Like Peptides-1 [Glp-1]
EP1799249A2 (fr) 2004-09-10 2007-06-27 Neose Technologies, Inc. Interferon alpha glycopegyle
JP5948627B2 (ja) 2004-10-29 2016-07-20 レイショファーム ゲーエムベーハー 線維芽細胞成長因子（ｆｇｆ）のリモデリングと糖質ペグ化
EP2514757A3 (fr) 2005-01-10 2014-03-05 ratiopharm GmbH Facteur de stimulation de colonies de granulocytes glycopegylé
EP1841787A2 (fr) 2005-01-25 2007-10-10 Cell Therapeutics, Inc. Conjugues de proteine biologiquement actives possedant une demi-vie in vivo modifiee
US9187546B2 (en) 2005-04-08 2015-11-17 Novo Nordisk A/S Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants
EP1888098A2 (fr) 2005-05-25 2008-02-20 Neose Technologies, Inc. Formulations d'erythropoietine glycopegylees
EP1746161A1 (fr) * 2005-07-20 2007-01-24 Cytheris IL-7 glycosylée, préparation et utilisations
US20070105755A1 (en) 2005-10-26 2007-05-10 Neose Technologies, Inc. One pot desialylation and glycopegylation of therapeutic peptides
WO2007056191A2 (fr) 2005-11-03 2007-05-18 Neose Technologies, Inc. Purification de sucre de nucleotide en utilisant des membranes
WO2007111496A1 (fr) * 2006-03-28 2007-10-04 Universiteit Utrecht Holding B.V. Domaines de reconnaissance d'hydrates de carbone améliorés
WO2008011633A2 (fr) 2006-07-21 2008-01-24 Neose Technologies, Inc. Glycosylation de peptides par l'intermédiaire de séquences de glycosylation à liaison o
US20100075375A1 (en) 2006-10-03 2010-03-25 Novo Nordisk A/S Methods for the purification of polypeptide conjugates
PE20081250A1 (es) * 2006-11-28 2008-10-07 Centelion FUSIONES Fc CON RECEPTOR PARA FGF SOLUBLE MODIFICADAS, CON ACTIVIDAD BIOLOGICA MEJORADA
KR20100016160A (ko) 2007-04-03 2010-02-12 바이오제너릭스 에이지 글리코페길화 ｇ―ｃｓｆ를 이용하는 치료 방법
JP5876649B2 (ja) 2007-06-12 2016-03-02 ラツィオファルム ゲーエムベーハーｒａｔｉｏｐｈａｒｍ ＧｍｂＨ ヌクレオチド糖の改良製造法
DK2173890T3 (da) 2007-06-21 2011-06-27 Univ Muenchen Tech Biologisk aktive proteiner med forhøjet stabilitet in vivo og/eller in vitro
US8207112B2 (en) 2007-08-29 2012-06-26 Biogenerix Ag Liquid formulation of G-CSF conjugate
JP5769142B2 (ja) * 2007-09-04 2015-08-26 独立行政法人酒類総合研究所 ピヒア属酵母において異種タンパク質を高分泌させる方法
WO2009108806A1 (fr) 2008-02-27 2009-09-03 Novo Nordisk A/S Molécules de facteur viii conjuguées
JP2010183885A (ja) * 2009-02-13 2010-08-26 Kobe Univ タンパク質の製造方法およびそれに用いる発現ベクター
CN105477641B (zh) 2010-05-21 2021-03-23 Xl-蛋白有限责任公司 生物合成的脯氨酸/丙氨酸无规卷曲多肽及其用途
CN105296447A (zh) * 2010-11-08 2016-02-03 阿米库斯治疗学公司 具有增强的稳定性和增强的保留催化活性的变体重组β-葡萄糖脑苷脂酶蛋白
US20160296632A1 (en) * 2013-11-13 2016-10-13 Aequus Biopharma, Inc. Engineered glycoproteins and uses thereof
WO2015091130A1 (fr) * 2013-12-20 2015-06-25 F. Hoffmann-La Roche Ag Procédé pour l'amélioration de la production recombinante de polypeptides de fusion solubles
US10370692B2 (en) 2013-12-20 2019-08-06 Hoffmann-La Roche Inc. Recombinant polypeptide production methods
PL3313879T3 (pl) 2015-06-24 2022-04-11 F. Hoffmann-La Roche Ag Przeciwciała przeciwko receptorowi transferyny z dostosowanym powinowactwem
MX2018003822A (es) 2015-10-02 2018-06-22 Hoffmann La Roche Anticuerpos biespecificos contra el cd20 humano y el receptor de transferrina humano y metodos de uso.
AR106189A1 (es) 2015-10-02 2017-12-20 Hoffmann La Roche ANTICUERPOS BIESPECÍFICOS CONTRA EL A-b HUMANO Y EL RECEPTOR DE TRANSFERRINA HUMANO Y MÉTODOS DE USO
MA47436A (fr) * 2017-01-31 2019-12-11 Regenxbio Inc Traitement de la mucopolysaccharidose i avec une alpha-l-iduronidase glycosylée entièrement humaine (idua)
US11890329B2 (en) 2017-07-06 2024-02-06 The Trustees Of The University Of Pennsylvania AAV9-mediated gene therapy for treating mucopolysaccharidosis type I
SG11202002457RA (en) 2017-09-22 2020-04-29 Univ Pennsylvania Gene therapy for treating mucopolysaccharidosis type ii

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5218092A (en) * 1988-09-29 1993-06-08 Kyowa Hakko Kogyo Co., Ltd. Modified granulocyte-colony stimulating factor polypeptide with added carbohydrate chains
JP2893481B2 (ja) * 1988-12-23 1999-05-24 ジェンザイム コーポレイション 酵素的に活性な組換えグルコセレブロシダーゼ
ZA987497B (en) * 1997-09-08 1999-02-23 Akzo Nobel Nv Expression of gonadotropins in dictyostelium
ATE458007T1 (de) * 1998-04-20 2010-03-15 Glycart Biotechnology Ag Glykosylierungs-engineering von antikörpern zur verbesserung der antikörperabhängigen zellvermittelten zytotoxizität

Also Published As

Publication number Publication date
WO2002002597A2 (fr) 2002-01-10
JP2004504016A (ja) 2004-02-12
WO2002002597A3 (fr) 2002-06-27
AU2001267337A1 (en) 2002-01-14
EP1299535A2 (fr) 2003-04-09

Similar Documents

Publication Publication Date Title
CA2412882A1 (fr) Polypeptides glycosyles a extension peptidique
US20060160177A1 (en) Peptide extended glycosylated polypeptides
US20200330587A1 (en) Epstein-barr virus vaccines
US11479605B2 (en) Homodimeric protein constructs
US20210284698A1 (en) Stabilized soluble pre-fusion rsv f polypeptides
EP3180020B1 (fr) Variants d'il-2 modifiés qui activent sélectivement les cellules t régulatrices pour le traitement de maladies auto-immunes
US11759514B2 (en) Stabilized pre-fusion RSV F proteins
US20020198162A1 (en) Antigen library immunization
US20190016760A1 (en) Multimerization of Recombinant Protein by Fusion to a Sequence from Lamprey
WO2004044006A1 (fr) Conjugues de l'interleukine-10 et de polymeres
TW201438743A (zh) 糖鏈加成連接基、含有糖鏈加成連接基與生理活性物質的化合物或其鹽，以及其製造方法
CN111514286B (zh) 一种寨卡病毒e蛋白结合疫苗及其制备方法
CN114173809A (zh) 免疫刺激组合物及其用途
WO2013177440A2 (fr) Réseau de flagelline ordonné utilisé comme immunostimulant
Ebrahimi et al. Fusion and sequence analysis of the influenza A (H9N2) virus M2e and C-terminal fragment of Mycobacterium tuberculosis HSP70 (H37Rv).
WO2023282771A1 (fr) Composition de vaccin contre le coronavirus
CN115368468A (zh) 一种新型冠状病毒SARS-CoV-2突变体疫苗与应用
JPWO2005090576A1 (ja) 百日咳感染症予防用ｄｎａ構築物
MXPA00007892A (en) Antigen library immunization

Legal Events

Date Code Title Description
FZDE Dead