CA2402588A1 - Taxonomic identification of pathogenic microorganisms and their toxic proteins - Google Patents
Taxonomic identification of pathogenic microorganisms and their toxic proteins Download PDFInfo
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- CA2402588A1 CA2402588A1 CA002402588A CA2402588A CA2402588A1 CA 2402588 A1 CA2402588 A1 CA 2402588A1 CA 002402588 A CA002402588 A CA 002402588A CA 2402588 A CA2402588 A CA 2402588A CA 2402588 A1 CA2402588 A1 CA 2402588A1
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- 230000001681 protective effect Effects 0.000 description 1
- 231100000654 protein toxin Toxicity 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 229950003776 protoporphyrin Drugs 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
- 244000000070 pulmonary pathogen Species 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 208000000995 spontaneous abortion Diseases 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 1
- 208000006379 syphilis Diseases 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229940118376 tetanus toxin Drugs 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000004454 trace mineral analysis Methods 0.000 description 1
- 201000008297 typhoid fever Diseases 0.000 description 1
- 150000003668 tyrosines Chemical class 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- LLMKLMMXMOTPRU-YOAXHERRSA-N vibriobactin Chemical compound O=C([C@@H]1N=C(O[C@H]1C)C=1C(=C(O)C=CC=1)O)NCCCN(C(=O)[C@@H]1[C@H](OC(=N1)C=1C(=C(O)C=CC=1)O)C)CCCNC(=O)C1=CC=CC(O)=C1O LLMKLMMXMOTPRU-YOAXHERRSA-N 0.000 description 1
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- JHYVWAMMAMCUIR-VQNLDRKJSA-N yersiniabactin Chemical compound C([C@@H](N=1)C2SC[C@H](N2)[C@@H](O)C(C)(C)C=2SC[C@@](C)(N=2)C(O)=O)SC=1C1=CC=CC=C1O JHYVWAMMAMCUIR-VQNLDRKJSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
- B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
- B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
- B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/999,159 US6780602B2 (en) | 2001-11-01 | 2001-11-01 | Taxonomic identification of pathogenic microorganisms and their toxic proteins |
| US09/999,159 | 2001-11-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2402588A1 true CA2402588A1 (en) | 2003-05-01 |
Family
ID=25545977
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002402588A Abandoned CA2402588A1 (en) | 2001-11-01 | 2002-09-10 | Taxonomic identification of pathogenic microorganisms and their toxic proteins |
Country Status (7)
| Country | Link |
|---|---|
| US (5) | US6780602B2 (enExample) |
| EP (1) | EP1308520A3 (enExample) |
| JP (5) | JP2003185668A (enExample) |
| CN (1) | CN1417347A (enExample) |
| CA (1) | CA2402588A1 (enExample) |
| HK (1) | HK1054054A1 (enExample) |
| SG (5) | SG152039A1 (enExample) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10138072A1 (de) * | 2001-08-03 | 2003-02-27 | Fraunhofer Ges Forschung | Verfahren und Vorrichtung zum Bestimmen von Proteinen auf einem Reaktionsträger |
| US6780602B2 (en) * | 2001-11-01 | 2004-08-24 | Microbiosystems, Limited Partnership | Taxonomic identification of pathogenic microorganisms and their toxic proteins |
| CN100362021C (zh) * | 2003-06-06 | 2008-01-16 | 河南大学 | 山药多糖-铁复合物及其制备工艺 |
| US20070099259A1 (en) * | 2003-12-15 | 2007-05-03 | Rapid Laboratory Microsystems Inc. | Electrochemical assay for the identification of microorganisms |
| JP4686703B2 (ja) * | 2004-10-29 | 2011-05-25 | 国立大学法人 名古屋工業大学 | 細胞固定化材 |
| US20070141597A1 (en) * | 2005-10-25 | 2007-06-21 | Harmon H J | Biomimetic Biodetector of Toxins, Viruses, Bacteria, and Biological Factors |
| JP2008249534A (ja) * | 2007-03-30 | 2008-10-16 | Sanyo Chem Ind Ltd | 免疫測定用担体 |
| EP2197350A2 (en) * | 2007-09-11 | 2010-06-23 | Baxter International Inc. | Infusion therapy sensor system |
| AU2009296526B2 (en) * | 2008-09-24 | 2015-11-19 | First Light Diagnostics, Inc. | Method for detecting analytes |
| MX2011004108A (es) | 2008-10-31 | 2011-08-15 | Bio Merieux Inc | Metodos para la separacion, caracterizacion y/o identificacion de microorganismos utilizando espectrometria de masas. |
| EP2364356B1 (en) * | 2008-10-31 | 2019-06-19 | Biomerieux, Inc | Methods for detection and identification of microorganisms |
| CA2740836C (en) * | 2008-10-31 | 2017-03-21 | Biomerieux, Inc. | Methods for separation, characterization and/or identification of microorganisms using spectroscopy |
| JP2012507710A (ja) * | 2008-10-31 | 2012-03-29 | バイオメリュー・インコーポレイテッド | 同定作用物質を使用した微生物の分離、キャラクタリゼーションおよび／または同定方法 |
| CN102272602B (zh) * | 2008-10-31 | 2014-03-12 | 生物梅里埃公司 | 微生物的分离和鉴定方法 |
| WO2010062351A1 (en) * | 2008-10-31 | 2010-06-03 | Biomerieux, Inc. | Methods for separation, characterization, and/or identification of microorganisms using raman spectroscopy |
| US8647835B2 (en) | 2008-10-31 | 2014-02-11 | BIO MéRIEUX, INC. | Methods for separation, characterization and/or identification of microorganisms using spectroscopy |
| JP5837420B2 (ja) | 2008-12-16 | 2015-12-24 | ビオメリュー・インコーポレイテッド | 固体又は半固体培地上の微生物のキャラクタリゼーション方法 |
| BR112012028926B1 (pt) | 2010-05-14 | 2021-02-02 | Biomerieux, Inc | método e aparelho para rápida identificação e/ou caracterização de um agente microbiano presente em uma amostra e computador |
| US8666673B2 (en) | 2010-05-14 | 2014-03-04 | Biomerieux, Inc | Identification and/or characterization of a microbial agent using taxonomic hierarchical classification |
| CA2803348A1 (en) | 2010-06-25 | 2012-04-19 | Purdue Research Foundation | Pathogen detection |
| CN104697989B (zh) * | 2013-12-06 | 2017-08-25 | 中国科学院理化技术研究所 | 含Fe3+的复合黏土的制备及其变色法检测水体生物毒性的方法 |
| US9880159B2 (en) | 2014-10-01 | 2018-01-30 | Linda S. Powers | Cartridge-based detection system |
| AU2016245213A1 (en) * | 2015-04-09 | 2017-11-23 | Koninklijke Philips N.V. | Method and apparatus for estimating the quantity of microorganisms within a taxonomic unit in a sample |
| US9867250B1 (en) | 2015-04-20 | 2018-01-09 | The Arizona Board Of Regents On Behalf Of The University Of Arizona | System having a configurable pico-second pulsed LED driver circuit and photomultiplier tube biasing and gating circuits for real-time, time-resolved transient recording of fluorescence |
| EP3551034A1 (en) * | 2016-12-07 | 2019-10-16 | Progenity, Inc. | Gastrointestinal tract detection methods, devices and systems |
| PT3554681T (pt) | 2016-12-16 | 2022-03-21 | Brigham & Womens Hospital Inc | Método para matriz de sensores de coroa de proteínas para a deteção precoce de doenças |
| US20210072255A1 (en) | 2016-12-16 | 2021-03-11 | The Brigham And Women's Hospital, Inc. | System and method for protein corona sensor array for early detection of diseases |
| CN119804399A (zh) | 2018-04-19 | 2025-04-11 | 曙光诊断学公司 | 靶标检测 |
| GB2595583A (en) | 2018-11-07 | 2021-12-01 | Seer Inc | Compositions, methods and systems for protein corona analysis and uses thereof |
| CA3131402A1 (en) | 2019-03-26 | 2020-10-01 | Xiaoyan Zhao | Compositions, methods and systems for protein corona analysis from biofluids and uses thereof |
| KR20240137114A (ko) | 2019-08-05 | 2024-09-19 | 시어 인코퍼레이티드 | 샘플 제조, 데이터 생성, 및 단백질 코로나 분석을 위한 시스템 및 방법 |
| JP2023540904A (ja) | 2020-08-25 | 2023-09-27 | シアー， インコーポレイテッド | タンパク質および核酸をアッセイするための組成物および方法 |
| CN114359897A (zh) * | 2020-10-13 | 2022-04-15 | 深圳赛威玛智能科技有限公司 | 病原微生物的智能识别方法、计算机设备及可读存储介质 |
| CN117225491B (zh) * | 2023-11-15 | 2024-01-16 | 拜奥智能科技(山东)有限公司 | 一种可减少氨残留的定氮仪防溅瓶 |
Family Cites Families (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5958790A (en) | 1984-12-20 | 1999-09-28 | Nycomed Imaging As | Solid phase transverse diffusion assay |
| US5089395A (en) | 1985-02-27 | 1992-02-18 | University Of Cincinnati | Viable microorganism detection by induced fluorescence |
| US4746604A (en) | 1985-05-24 | 1988-05-24 | Enzo Biochem, Inc. | Specific binding assays utilizing a viable cell as a label |
| US5415997A (en) | 1987-07-28 | 1995-05-16 | Biotechnology Australia Pty Limited | Method for detecting low levels of microorganisms |
| US4953561A (en) * | 1989-09-18 | 1990-09-04 | Cancer Diagnostics, Inc. | Urine testing module and method of collecting urine antigen |
| US5766933A (en) * | 1989-04-26 | 1998-06-16 | Diagnostic Products Corporation | Method and element for measuring analytes in biological fluids using immobilized binder--analyte labeled complex |
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US6017722A (en) | 1991-04-04 | 2000-01-25 | Board Of Regents, The University Of Texas System | Luminous bacteria and methods for the isolation, identification and quantitation of toxicants |
| CA2107899A1 (en) * | 1991-04-10 | 1992-10-11 | Gunars Edwin Valkirs | Novel conjugates and assays for simultaneous detection of multiple ligands |
| DK39592D0 (da) | 1992-03-25 | 1992-03-25 | Safefood Micro Systsm As | Method of detecting microorganisms |
| GB9212416D0 (en) | 1992-06-11 | 1992-07-22 | Medical Res Council | Reversible binding substances |
| ATE162892T1 (de) | 1992-07-31 | 1998-02-15 | Behringwerke Ag | Photoaktivierbare chemiluminizierende matrices |
| US6001556A (en) | 1992-11-13 | 1999-12-14 | The Regents Of The University Of California | Polymeric assay film for direct colorimetric detection |
| US5576220A (en) * | 1993-02-19 | 1996-11-19 | Arris Pharmaceutical Corporation | Thin film HPMP matrix systems and methods for constructing and displaying ligands |
| US5415999A (en) | 1993-07-09 | 1995-05-16 | Biocircuits Corporation | Fluorescent lipid polymer-macromolecular ligand compositions as detection element in ligand assays |
| US5986076A (en) | 1994-05-11 | 1999-11-16 | Trustees Of Boston University | Photocleavable agents and conjugates for the detection and isolation of biomolecules |
| WO1995031481A1 (en) | 1994-05-18 | 1995-11-23 | The Research And Development Institute, Inc. | Simple, rapid method for the detection, identification and enumeration of specific viable microorganisms |
| AUPN214095A0 (en) | 1995-04-03 | 1995-04-27 | Australian Water Technologies Pty Ltd | Method for detecting microorganisms using flow cytometry |
| US5731148A (en) | 1995-06-07 | 1998-03-24 | Gen-Probe Incorporated | Adduct protection assay |
| US6159689A (en) | 1995-11-10 | 2000-12-12 | Genera Technologies Limited | Methods of capture and assay procedures |
| US5741662A (en) | 1995-12-18 | 1998-04-21 | Quidel Corporation | Direct stain specific binding assays for microorganisms |
| GB9608540D0 (en) | 1996-04-25 | 1996-07-03 | Medical Res Council | Isolation of enzymes |
| WO1997044491A1 (en) | 1996-05-22 | 1997-11-27 | The Johns Hopkins University | Methods of detection utilizing modified bacteriophage |
| US5760406A (en) * | 1996-06-03 | 1998-06-02 | Powers; Linda | Method and apparatus for sensing the presence of microbes |
| US5965532A (en) * | 1996-06-28 | 1999-10-12 | Trustees Of Tufts College | Multivalent compounds for crosslinking receptors and uses thereof |
| US5972715A (en) | 1996-12-23 | 1999-10-26 | Bayer Corporation | Use of thermochromic liquid crystals in reflectometry based diagnostic methods |
| US5888066A (en) * | 1997-01-28 | 1999-03-30 | Diro, Inc. | Converter assembly for threaded dental implants |
| FR2758884B1 (fr) * | 1997-01-30 | 1999-04-02 | Bio Merieux | Procede pour isoler, notamment detecter ou quantifier un analyte dans un milieu |
| US6087088A (en) | 1997-01-31 | 2000-07-11 | Bayer Corporation | Binding assays using more than one label for determining analyte in the presence of interfering factors |
| US5763158A (en) | 1997-02-06 | 1998-06-09 | The United States Of America As Represented By The Secretary Of The Army | Detection of multiple antigens or antibodies |
| US5888760A (en) | 1997-04-10 | 1999-03-30 | Dade Microscan Inc. | Universal test systems and methods of use thereof for identifying multiple families of microorganisms |
| CZ297165B6 (cs) * | 1997-04-21 | 2006-09-13 | Randox Laboratories Ltd. A British Company Of Ardmore | Zpusob výroby zarízení s pevnou fází k provádení multianalytních rozboru |
| AU7164998A (en) * | 1997-04-28 | 1998-11-24 | B-E Safe, Inc. | Taxonomic identification of microorganisms, proteins and peptides involved in vertebrate disease states |
| US6197568B1 (en) | 1997-07-29 | 2001-03-06 | The Regents Of The University Of Michigan | Method and compositions for isolation, diagnosis and treatment of polyanion-binding microorganisms |
| CA2307123C (en) | 1997-10-27 | 2007-04-24 | Idexx Laboratories, Inc. | Device and methods for determination of analyte in a solution |
| US5976827A (en) | 1997-12-12 | 1999-11-02 | Akzo Nobel, N.V. | Sensor device for detecting microorganisms and method therefor |
| US5912115A (en) | 1997-12-12 | 1999-06-15 | Akzo Nobel, N.V. | Evacuated sensor device for detecting microorganisms in blood samples, and method thereof |
| US6214628B1 (en) | 1998-01-14 | 2001-04-10 | Joseph R. Lakowicz | Method of conducting an assay of a sample containing an analyte of interest |
| US5968766A (en) * | 1998-03-31 | 1999-10-19 | B.E. Safe | Method and apparatus for sensing the presence of microbes |
| WO1999064036A1 (en) * | 1998-06-08 | 1999-12-16 | Advanced Medicine, Inc. | Novel therapeutic agents for macromolecular structures |
| US6051388A (en) | 1998-12-22 | 2000-04-18 | Toxin Alert, Inc. | Method and apparatus for selective biological material detection |
| WO2000068689A1 (en) * | 1999-05-10 | 2000-11-16 | Prolinx Inc. | Cell separation device and methods for use |
| US6699677B1 (en) * | 1999-12-20 | 2004-03-02 | Chemocentryx, Inc. | Tethered ligands and methods of use |
| US6395504B1 (en) * | 2000-09-01 | 2002-05-28 | New Horizons Diagnostics Corp. | Use of phage associated lytic enzymes for the rapid detection of bacterial contaminants |
| US20020187464A1 (en) * | 2000-09-22 | 2002-12-12 | Klempner Mark S. | Microarray-based method for rapid identification of cells, microorganisms, or protein mixtures |
| EP2202520A1 (en) * | 2000-11-27 | 2010-06-30 | Intelligent Medical Devices LLC | Clinically intelligent diagnostic devices and methods |
| US6780602B2 (en) * | 2001-11-01 | 2004-08-24 | Microbiosystems, Limited Partnership | Taxonomic identification of pathogenic microorganisms and their toxic proteins |
| US20060257929A1 (en) * | 2003-11-12 | 2006-11-16 | Microbiosystems, Limited Partnership | Method for the rapid taxonomic identification of pathogenic microorganisms and their toxic proteins |
-
2001
- 2001-11-01 US US09/999,159 patent/US6780602B2/en not_active Expired - Fee Related
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2002
- 2002-09-10 CA CA002402588A patent/CA2402588A1/en not_active Abandoned
- 2002-09-27 SG SG200407090-0A patent/SG152039A1/en unknown
- 2002-09-27 SG SG200406358-2A patent/SG152902A1/en unknown
- 2002-09-27 CN CN02143520A patent/CN1417347A/zh active Pending
- 2002-09-27 SG SG200407091-8A patent/SG143054A1/en unknown
- 2002-09-27 SG SG200407092-6A patent/SG145552A1/en unknown
- 2002-09-27 EP EP02021593A patent/EP1308520A3/en not_active Ceased
- 2002-09-27 SG SG200205915-2A patent/SG130937A1/en unknown
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2003
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- 2004-05-20 JP JP2004151016A patent/JP2004309494A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| SG145552A1 (en) | 2008-09-29 |
| JP2004333503A (ja) | 2004-11-25 |
| SG152039A1 (en) | 2009-05-29 |
| US20030124532A1 (en) | 2003-07-03 |
| US20050026141A1 (en) | 2005-02-03 |
| EP1308520A3 (en) | 2003-11-12 |
| JP2004309494A (ja) | 2004-11-04 |
| US20050026140A1 (en) | 2005-02-03 |
| US6780602B2 (en) | 2004-08-24 |
| JP2003185668A (ja) | 2003-07-03 |
| CN1417347A (zh) | 2003-05-14 |
| SG152902A1 (en) | 2009-06-29 |
| JP2004309493A (ja) | 2004-11-04 |
| US20050032115A1 (en) | 2005-02-10 |
| HK1054054A1 (zh) | 2003-11-14 |
| SG130937A1 (en) | 2007-04-26 |
| SG143054A1 (en) | 2008-06-27 |
| US20040096910A1 (en) | 2004-05-20 |
| JP2004317520A (ja) | 2004-11-11 |
| EP1308520A2 (en) | 2003-05-07 |
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