CA2397610C - Analyse in vivo pour tester la stabilite phenotypique - Google Patents

Analyse in vivo pour tester la stabilite phenotypique Download PDF

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Publication number
CA2397610C
CA2397610C CA2397610A CA2397610A CA2397610C CA 2397610 C CA2397610 C CA 2397610C CA 2397610 A CA2397610 A CA 2397610A CA 2397610 A CA2397610 A CA 2397610A CA 2397610 C CA2397610 C CA 2397610C
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Canada
Prior art keywords
cells
cartilage
chondrocytes
cell
markers
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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CA2397610A
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English (en)
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CA2397610A1 (fr
Inventor
Frank Luyten
Cosimo De Bari
Francesco Dell'accio
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Tigenix NV
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Tigenix NV
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Publication of CA2397610A1 publication Critical patent/CA2397610A1/fr
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Publication of CA2397610C publication Critical patent/CA2397610C/fr
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
    • A61K49/0006—Skin tests, e.g. intradermal testing, test strips, delayed hypersensitivity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
    • A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/04—Drugs for skeletal disorders for non-specific disorders of the connective tissue
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pathology (AREA)
  • Rheumatology (AREA)
  • Toxicology (AREA)
  • Urology & Nephrology (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Zoology (AREA)
  • Dermatology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

L'invention se rapporte à une analyse in vivo de mesure de la croissance, indépendamment de l'ancrage, et de la stabilité phénotypique d'une population de cellules. Cette analyse consiste à injecter à un mammifère, de manière sous-cutanée ou intramusculaire, une suspension de cellules de chondrocytes articulaires dans un liquide iso-osmotique, la même suspension contenant des chondrocytes articulaires en quantité équivalente à au moins 1 x 10?6¿ chondrocytes lorsqu'elle est appliquée à une souris immunodéficiente. Le résultat est lié aux marqueurs moléculaires. La présente invention se rapporte également à des puces à ADN et à des outils diagnostiques comportant de telles puces, qui permettent de prédire le résultat d'une transplantation autologue de chondrocytes. Il est également possible d'utiliser des anticorps dirigés contre des marqueurs positifs et négatifs de la stabilité des chondrocytes aux fins d'un contrôle de qualité sur ces chondrocytes. Les compositions thérapeutiques comportant des chondrocytes stables sont très utiles pour les réparations tissulaires.
CA2397610A 1999-10-06 2000-10-06 Analyse in vivo pour tester la stabilite phenotypique Expired - Lifetime CA2397610C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP99203273 1999-10-06
EP99203273.0 1999-10-06
PCT/BE2000/000118 WO2001024833A2 (fr) 1999-10-06 2000-10-06 Analyse in vivo et marqueurs moleculaires permettant de tester la stabilite phenotypique de populations de cellules et de selectionner des populations de cellules destinees a une transplantation autologue

Publications (2)

Publication Number Publication Date
CA2397610A1 CA2397610A1 (fr) 2001-04-12
CA2397610C true CA2397610C (fr) 2012-07-03

Family

ID=8240723

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2397610A Expired - Lifetime CA2397610C (fr) 1999-10-06 2000-10-06 Analyse in vivo pour tester la stabilite phenotypique

Country Status (12)

Country Link
US (2) US7479367B1 (fr)
EP (2) EP1218037B1 (fr)
AT (1) ATE285797T1 (fr)
AU (1) AU7764200A (fr)
CA (1) CA2397610C (fr)
CY (1) CY2010007I1 (fr)
DE (3) DE60017159T2 (fr)
DK (1) DK1218037T3 (fr)
ES (2) ES2234673T3 (fr)
LU (1) LU91672I2 (fr)
PT (1) PT1218037E (fr)
WO (1) WO2001024833A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7482114B2 (en) 1999-10-06 2009-01-27 Tigenix N.V. In vivo assay and molecular markers for testing the phenotypic stability of cell populations, and selected cell populations for autologous transplantation
US7479367B1 (en) * 1999-10-06 2009-01-20 Tigenex N.V. In vivo assay and molecular markers for testing the phenotypic stability of cell populations and selected cell populations for autologous transplantation
AU2001266915A1 (en) 2000-06-14 2001-12-24 Case Western Reserve University Probes for chondrogenesis
US7462448B2 (en) * 2002-08-02 2008-12-09 Stratatech Corporation Species specific DNA detection
US20100028308A1 (en) 2006-03-20 2010-02-04 Tigenix N.V. Methods to maintain, improve and restore the cartilage phenotype of chondrocytes
ES2452825T3 (es) 2006-11-24 2014-04-02 Tigenix N.V. Genes marcadores para su uso en la identificación de estabilidad fenotípica de condrocitos y en el cribado de factores que influyen en la producción de cartílago
CN101215565B (zh) * 2008-01-11 2011-04-27 华中农业大学 一种作为猪标记辅助选择的与免疫性状相关的分子标记的克隆及应用
GB0902793D0 (en) 2009-02-20 2009-04-08 Univ Gent GDF15 as molecular tool to monitor and enhance phenotypic stability of articular chondrocytes
EP3872168A1 (fr) * 2020-02-28 2021-09-01 Cline Scientific AB Différenciation de chondrocytes
CN116323666A (zh) 2020-08-21 2023-06-23 建新公司 Fgfr3抗体和使用方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837258A (en) * 1991-08-30 1998-11-17 University Of South Florida Induction of tissue, bone or cartilage formation using connective tissue growth factor
US5723331A (en) 1994-05-05 1998-03-03 Genzyme Corporation Methods and compositions for the repair of articular cartilage defects in mammals
US5902785A (en) 1995-06-06 1999-05-11 Genetics Institute, Inc. Cartilage induction by bone morphogenetic proteins
DE69619526T2 (de) 1995-06-12 2002-10-31 Yeda Research And Development Co., Ltd. Fgf9 als spezifischer ligand für fgfr3
JP2002504812A (ja) 1997-05-21 2002-02-12 クローンテック ラボラトリーズ，インコーポレイテッド 核酸アレイ
AU7984498A (en) * 1997-06-25 1999-01-04 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Serum-free cell growth medium
DE20023640U1 (de) * 1999-10-06 2005-06-23 Tigenix N.V. Isolierung von Vorläuferzellen und deren Verwendung zum Wiederaufbau von Bindegewebe
US7482114B2 (en) 1999-10-06 2009-01-27 Tigenix N.V. In vivo assay and molecular markers for testing the phenotypic stability of cell populations, and selected cell populations for autologous transplantation
US7479367B1 (en) * 1999-10-06 2009-01-20 Tigenex N.V. In vivo assay and molecular markers for testing the phenotypic stability of cell populations and selected cell populations for autologous transplantation

Also Published As

Publication number Publication date
EP1498146A3 (fr) 2007-01-24
ATE285797T1 (de) 2005-01-15
US7479367B1 (en) 2009-01-20
ES2488240T1 (es) 2014-08-26
US9089598B2 (en) 2015-07-28
DE122010000021I1 (de) 2012-09-06
EP1218037A2 (fr) 2002-07-03
PT1218037E (pt) 2005-05-31
US20090162328A1 (en) 2009-06-25
EP1218037B1 (fr) 2004-12-29
LU91672I2 (fr) 2010-06-02
WO2001024833A2 (fr) 2001-04-12
EP1498146A2 (fr) 2005-01-19
WO2001024833A3 (fr) 2001-12-20
DE20023659U1 (de) 2005-07-07
DE60017159T2 (de) 2006-05-04
AU7764200A (en) 2001-05-10
ES2234673T3 (es) 2005-07-01
CY2010007I2 (el) 2010-07-28
DE60017159D1 (de) 2005-02-03
CY2010007I1 (el) 2010-07-28
CA2397610A1 (fr) 2001-04-12
DK1218037T3 (da) 2005-04-25

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