CA2395068A1 - Processes and organisms for the production of anti-freeze proteins - Google Patents
Processes and organisms for the production of anti-freeze proteins Download PDFInfo
- Publication number
- CA2395068A1 CA2395068A1 CA002395068A CA2395068A CA2395068A1 CA 2395068 A1 CA2395068 A1 CA 2395068A1 CA 002395068 A CA002395068 A CA 002395068A CA 2395068 A CA2395068 A CA 2395068A CA 2395068 A1 CA2395068 A1 CA 2395068A1
- Authority
- CA
- Canada
- Prior art keywords
- freeze
- sequence
- protein
- ice
- afp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 108010053481 Antifreeze Proteins Proteins 0.000 title claims abstract description 114
- 238000000034 method Methods 0.000 title claims description 34
- 230000008569 process Effects 0.000 title claims description 27
- 238000004519 manufacturing process Methods 0.000 title abstract description 14
- 241000320425 Marinomonas protea Species 0.000 claims abstract description 32
- 241000894006 Bacteria Species 0.000 claims abstract description 28
- 235000009508 confectionery Nutrition 0.000 claims abstract description 9
- 235000015243 ice cream Nutrition 0.000 claims abstract description 9
- 241000589516 Pseudomonas Species 0.000 claims abstract description 5
- 235000013311 vegetables Nutrition 0.000 claims abstract description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 56
- 102000004169 proteins and genes Human genes 0.000 claims description 49
- 238000012360 testing method Methods 0.000 claims description 30
- 230000001580 bacterial effect Effects 0.000 claims description 21
- 230000000694 effects Effects 0.000 claims description 20
- 230000002528 anti-freeze Effects 0.000 claims description 18
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 17
- 235000013305 food Nutrition 0.000 claims description 15
- 238000012258 culturing Methods 0.000 claims description 9
- 241001135624 Marinomonas Species 0.000 claims description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 8
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 claims description 4
- 108010029485 Protein Isoforms Proteins 0.000 claims description 3
- 102000001708 Protein Isoforms Human genes 0.000 claims description 3
- 235000013373 food additive Nutrition 0.000 claims description 3
- 239000002778 food additive Substances 0.000 claims description 3
- 150000007523 nucleic acids Chemical group 0.000 claims description 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims 3
- 235000013611 frozen food Nutrition 0.000 abstract description 6
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 38
- 210000004027 cell Anatomy 0.000 description 31
- 239000013078 crystal Substances 0.000 description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 28
- 235000002639 sodium chloride Nutrition 0.000 description 21
- 238000003556 assay Methods 0.000 description 17
- 239000000284 extract Substances 0.000 description 17
- 239000000203 mixture Substances 0.000 description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 16
- 238000001953 recrystallisation Methods 0.000 description 15
- 239000006228 supernatant Substances 0.000 description 15
- 239000008188 pellet Substances 0.000 description 14
- 239000000523 sample Substances 0.000 description 14
- 238000005119 centrifugation Methods 0.000 description 12
- 238000002360 preparation method Methods 0.000 description 12
- 238000000751 protein extraction Methods 0.000 description 12
- 239000011780 sodium chloride Substances 0.000 description 12
- 229930006000 Sucrose Natural products 0.000 description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 11
- 238000007710 freezing Methods 0.000 description 11
- 230000008014 freezing Effects 0.000 description 11
- 239000000463 material Substances 0.000 description 11
- 239000005720 sucrose Substances 0.000 description 11
- 239000007983 Tris buffer Substances 0.000 description 9
- 239000011324 bead Substances 0.000 description 9
- 230000005764 inhibitory process Effects 0.000 description 9
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 9
- 238000010438 heat treatment Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 101710123134 Ice-binding protein Proteins 0.000 description 7
- 239000011536 extraction buffer Substances 0.000 description 7
- 229940041514 candida albicans extract Drugs 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 239000012138 yeast extract Substances 0.000 description 6
- 241000251468 Actinopterygii Species 0.000 description 5
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- 239000001888 Peptone Substances 0.000 description 5
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- 239000004365 Protease Substances 0.000 description 5
- 238000009835 boiling Methods 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
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- 235000019319 peptone Nutrition 0.000 description 5
- 239000013535 sea water Substances 0.000 description 5
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
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- 108010091212 pepstatin Proteins 0.000 description 4
- FAXGPCHRFPCXOO-LXTPJMTPSA-N pepstatin A Chemical compound OC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)CC(C)C FAXGPCHRFPCXOO-LXTPJMTPSA-N 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
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- 241000588724 Escherichia coli Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
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- 239000000872 buffer Substances 0.000 description 3
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- UHZZMRAGKVHANO-UHFFFAOYSA-M chlormequat chloride Chemical compound [Cl-].C[N+](C)(C)CCCl UHZZMRAGKVHANO-UHFFFAOYSA-M 0.000 description 3
- 238000006073 displacement reaction Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
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- 238000000527 sonication Methods 0.000 description 3
- 239000001974 tryptic soy broth Substances 0.000 description 3
- 229940123779 Bacterial protease inhibitor Drugs 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- NHTMVDHEPJAVLT-UHFFFAOYSA-N Isooctane Chemical compound CC(C)CC(C)(C)C NHTMVDHEPJAVLT-UHFFFAOYSA-N 0.000 description 2
- 241001135625 Marinomonas communis Species 0.000 description 2
- 102000005741 Metalloproteases Human genes 0.000 description 2
- 108010006035 Metalloproteases Proteins 0.000 description 2
- 241000218902 Pseudomonas synxantha Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
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- 108010022999 Serine Proteases Proteins 0.000 description 2
- MGSKVZWGBWPBTF-UHFFFAOYSA-N aebsf Chemical compound NCCC1=CC=C(S(F)(=O)=O)C=C1 MGSKVZWGBWPBTF-UHFFFAOYSA-N 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
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- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 2
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- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- CGWBIHLHAGNJCX-UHFFFAOYSA-N 2-butylguanidine Chemical compound CCCCNC(N)=N CGWBIHLHAGNJCX-UHFFFAOYSA-N 0.000 description 1
- 102000004400 Aminopeptidases Human genes 0.000 description 1
- 108090000915 Aminopeptidases Proteins 0.000 description 1
- 102000035101 Aspartic proteases Human genes 0.000 description 1
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- VGGGPCQERPFHOB-MCIONIFRSA-N Bestatin Chemical compound CC(C)C[C@H](C(O)=O)NC(=O)[C@@H](O)[C@H](N)CC1=CC=CC=C1 VGGGPCQERPFHOB-MCIONIFRSA-N 0.000 description 1
- VGGGPCQERPFHOB-UHFFFAOYSA-N Bestatin Natural products CC(C)CC(C(O)=O)NC(=O)C(O)C(N)CC1=CC=CC=C1 VGGGPCQERPFHOB-UHFFFAOYSA-N 0.000 description 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 240000006766 Cornus mas Species 0.000 description 1
- 235000003363 Cornus mas Nutrition 0.000 description 1
- 102000005927 Cysteine Proteases Human genes 0.000 description 1
- 108010005843 Cysteine Proteases Proteins 0.000 description 1
- 102100035784 Decorin Human genes 0.000 description 1
- 108090000738 Decorin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 239000006137 Luria-Bertani broth Substances 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 241000577079 Marinomonas sp. Species 0.000 description 1
- 238000007476 Maximum Likelihood Methods 0.000 description 1
- 229920002274 Nalgene Polymers 0.000 description 1
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- 239000002033 PVDF binder Substances 0.000 description 1
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- 241000192142 Proteobacteria Species 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
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- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- BDMWLJLPPUCLNV-XGEHTFHBSA-N Ser-Thr-Val Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(O)=O BDMWLJLPPUCLNV-XGEHTFHBSA-N 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
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- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
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- 229940111685 dibasic potassium phosphate Drugs 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
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- 238000002270 exclusion chromatography Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000002194 freeze distillation Methods 0.000 description 1
- 235000013572 fruit purees Nutrition 0.000 description 1
- 238000012812 general test Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000010438 granite Substances 0.000 description 1
- 238000007542 hardness measurement Methods 0.000 description 1
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- -1 hypersaline) lakes Chemical compound 0.000 description 1
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- 235000015067 sauces Nutrition 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 239000007289 sea water agar Substances 0.000 description 1
- 235000011888 snacks Nutrition 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004781 supercooling Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/82—Proteins from microorganisms
- Y10S530/825—Bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Freezing, Cooling And Drying Of Foods (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9929696.4 | 1999-12-15 | ||
| GBGB9929696.4A GB9929696D0 (en) | 1999-12-15 | 1999-12-15 | Processes and organisms for the production of anti-freeze proteins |
| PCT/EP2000/012396 WO2001044275A2 (en) | 1999-12-15 | 2000-12-05 | Processes and organisms for the production of anti-freeze proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2395068A1 true CA2395068A1 (en) | 2001-06-21 |
Family
ID=10866404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002395068A Abandoned CA2395068A1 (en) | 1999-12-15 | 2000-12-05 | Processes and organisms for the production of anti-freeze proteins |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US6887984B2 (Direct) |
| EP (1) | EP1240188A2 (Direct) |
| CN (1) | CN1434859A (Direct) |
| AU (1) | AU774941B2 (Direct) |
| BR (1) | BR0016475A (Direct) |
| CA (1) | CA2395068A1 (Direct) |
| GB (1) | GB9929696D0 (Direct) |
| IL (1) | IL150232A0 (Direct) |
| IN (1) | IN189994B (Direct) |
| MX (1) | MXPA02005954A (Direct) |
| WO (1) | WO2001044275A2 (Direct) |
| ZA (1) | ZA200204816B (Direct) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2248819T3 (es) * | 1995-07-05 | 2006-03-16 | Unilever N.V. | Peptidos anticongelantes de peces marinos como aditivos para productos alimentarios. |
| AU2002352231A1 (en) * | 2002-01-04 | 2003-07-15 | Unilever Plc | Antifreeze proteins in vegetables |
| US7012475B2 (en) | 2003-12-30 | 2006-03-14 | Raytheon Company | Method and apparatus for effecting high-frequency amplification or oscillation |
| EP1809120B1 (en) * | 2004-10-18 | 2013-06-26 | Unilever PLC | Low fat frozen confectionery product |
| ATE550948T1 (de) | 2006-10-20 | 2012-04-15 | Nestec Sa | Eisstrukturierende peptiden aus milch |
| WO2009065415A1 (en) | 2007-11-21 | 2009-05-28 | Roskilde Universitet | Polypeptides comprising an ice-binding activity |
| CN101695346B (zh) * | 2009-09-25 | 2011-10-26 | 徐州工程学院 | 一种冷冻食品基料-地衣培养液的制备与应用方法 |
| US9394327B1 (en) * | 2013-03-15 | 2016-07-19 | The Trustees Of California State University | Nucleoside crystals, crystal nucleation and growth control with antifreeze proteins |
| CN103710288B (zh) * | 2013-12-26 | 2015-09-30 | 中国海洋大学 | 一种芳基硫酸酯酶的生产菌株及其应用 |
| WO2016193292A1 (en) * | 2015-06-02 | 2016-12-08 | Dsm Ip Assets B.V. | Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food |
| IT201900014121A1 (it) | 2019-08-06 | 2021-02-06 | Univ Degli Studi Di Camerino | Marinomonas ef1 e rhodococcus ef1 per la produzione di metaboliti secondari |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2248819T3 (es) | 1995-07-05 | 2006-03-16 | Unilever N.V. | Peptidos anticongelantes de peces marinos como aditivos para productos alimentarios. |
| JP4175520B2 (ja) | 1996-07-26 | 2008-11-05 | ユニリーバー・ナームローゼ・ベンノートシャープ | 冷凍菓子製品 |
| KR20000029565A (ko) * | 1996-07-26 | 2000-05-25 | 알 브이 테이트 (로드니 비버스 테이트);에이치 드로이. 씨. 지. 오닌크;이. 에디, 산드라 웨드워즈 (에스 제이 에드워즈) | 빙결억제펩티드를함유한냉동식품 |
| GB9801408D0 (en) | 1998-01-22 | 1998-03-18 | Unilever Plc | Frozen food product |
-
1999
- 1999-12-15 GB GBGB9929696.4A patent/GB9929696D0/en not_active Ceased
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2000
- 2000-12-05 CA CA002395068A patent/CA2395068A1/en not_active Abandoned
- 2000-12-05 AU AU26726/01A patent/AU774941B2/en not_active Ceased
- 2000-12-05 BR BR0016475-5A patent/BR0016475A/pt not_active IP Right Cessation
- 2000-12-05 MX MXPA02005954A patent/MXPA02005954A/es unknown
- 2000-12-05 IL IL15023200A patent/IL150232A0/xx unknown
- 2000-12-05 CN CN00819030A patent/CN1434859A/zh active Pending
- 2000-12-05 WO PCT/EP2000/012396 patent/WO2001044275A2/en not_active Ceased
- 2000-12-05 EP EP00989964A patent/EP1240188A2/en not_active Withdrawn
- 2000-12-14 IN IN1118MU2000 patent/IN189994B/en unknown
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| US6887984B2 (en) | 2005-05-03 |
| CN1434859A (zh) | 2003-08-06 |
| AU2672601A (en) | 2001-06-25 |
| IL150232A0 (en) | 2002-12-01 |
| ZA200204816B (en) | 2004-01-28 |
| IN189994B (Direct) | 2003-05-31 |
| EP1240188A2 (en) | 2002-09-18 |
| BR0016475A (pt) | 2002-11-12 |
| WO2001044275A3 (en) | 2002-03-21 |
| WO2001044275A2 (en) | 2001-06-21 |
| GB9929696D0 (en) | 2000-02-09 |
| MXPA02005954A (es) | 2002-10-23 |
| AU774941B2 (en) | 2004-07-15 |
| US20020072108A1 (en) | 2002-06-13 |
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