CA2378107A1 - Genes <i>dnd</i> ou de protection nucleotidique cyclique de <i>arabidopsis thaliana</i>; regulateurs de resistance de maladie des plantes et de mort cellulaire - Google Patents

Genes <i>dnd</i> ou de protection nucleotidique cyclique de <i>arabidopsis thaliana</i>; regulateurs de resistance de maladie des plantes et de mort cellulaire Download PDF

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Publication number
CA2378107A1
CA2378107A1 CA002378107A CA2378107A CA2378107A1 CA 2378107 A1 CA2378107 A1 CA 2378107A1 CA 002378107 A CA002378107 A CA 002378107A CA 2378107 A CA2378107 A CA 2378107A CA 2378107 A1 CA2378107 A1 CA 2378107A1
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Canada
Prior art keywords
gene
cngc
leu
plant
dnd
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Abandoned
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CA002378107A
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English (en)
Inventor
Andrew F. Bent
I-Ching Yu
Steven J. Clough
Kevin A. Fengler
Roger K. Smith, Jr.
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Individual
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Wisconsin Alumni Research Foundation
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Publication of CA2378107A1 publication Critical patent/CA2378107A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La réponse de mort cellulaire connue comme la réponse d'hypersensibilité (HR) est une caractéristique centrale de la résistance gène pour gène aux maladies des plantes. Les plantes se défendent également contre les pathogènes via des réponses de défense à large spectre multigéniquement contrôlées telles que celles modulées par l'acide salicylique. Le locus <i>DND</i> (Defense, No Death) d'<i>Arabidopsis thaliana</i> régule l'importance de la résistance des maladies à large spectre contre une vaste gamme de pathogènes viraux, bactériens, oomycètes et fongiques. Les plantes auxquelles il manque un exemplaire fonctionnel du gène <i>DND1</i> ou <i>DND2</i> présentent une déficience au niveau de la mort cellulaire par réponse d'hypersensibilité, mais font preuve d'une résistance gène pour gène efficace contre les maladies. Les plantes auxquelles il manque un exemplaire fonctionnel du gène <i>DND1</i> ou <i>DND2</i> présentent également un phénotype renforcé de résistance aux maladies à large spectre. Les produits géniques <i>DND1</i> et <i>DND2</i> sont identiques à des ADN complémentaires déjà connus, en l'occurrence, l'<i>AtCNGC2</i> et l'<i>AtCNGC1</i> qui codent les protéines à canal ionique protégé par un nucléotide apparemment cyclique. L'identification des gènes <i>CNGC/DND</i> comme régulateurs de la résistance aux maladies et à la mort cellulaire, et la disponibilité de l'information de séquence génique de <i>CNGC/DND</i>, offrent de nouvelles possibilités de lutte contre une grande variété de maladies de plantes.
CA002378107A 1999-07-23 2000-07-24 Genes <i>dnd</i> ou de protection nucleotidique cyclique de <i>arabidopsis thaliana</i>; regulateurs de resistance de maladie des plantes et de mort cellulaire Abandoned CA2378107A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14531099P 1999-07-23 1999-07-23
US60/145,310 1999-07-23
PCT/US2000/020216 WO2001007596A1 (fr) 1999-07-23 2000-07-24 Genes dnd ou de protection nucleotidique cyclique de arabidopsis thaliana; regulateurs de resistance de maladie des plantes et de mort cellulaire

Publications (1)

Publication Number Publication Date
CA2378107A1 true CA2378107A1 (fr) 2001-02-01

Family

ID=22512507

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002378107A Abandoned CA2378107A1 (fr) 1999-07-23 2000-07-24 Genes <i>dnd</i> ou de protection nucleotidique cyclique de <i>arabidopsis thaliana</i>; regulateurs de resistance de maladie des plantes et de mort cellulaire

Country Status (6)

Country Link
EP (1) EP1196553A1 (fr)
JP (1) JP2003505076A (fr)
AU (1) AU6373300A (fr)
CA (1) CA2378107A1 (fr)
MX (1) MXPA02000781A (fr)
WO (1) WO2001007596A1 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8088971B2 (en) 2005-03-08 2012-01-03 Basf Plant Science Gmbh Expression enhancing intron sequences
CN102747090B (zh) * 2012-07-02 2014-06-11 中山大学 水稻抗病基因OsLYP4及其应用
US10000767B2 (en) 2013-01-28 2018-06-19 Monsanto Technology Llc Methods and compositions for plant pest control
US20140283211A1 (en) 2013-03-14 2014-09-18 Monsanto Technology Llc Methods and Compositions for Plant Pest Control
CN103789231B (zh) * 2014-01-07 2015-09-23 广西大学 一种利用离体叶脉片接种烟草青枯病的人工发病方法
CN103789215B (zh) * 2014-01-07 2015-10-21 广西大学 一种利用离体叶脉片接种烟草立枯病的人工发病方法
CN103789216B (zh) * 2014-01-07 2015-10-21 广西大学 一种烟草立枯病离体接种的人工发病方法
CN103805539B (zh) * 2014-01-07 2015-09-23 广西大学 一种烟草青枯病离体接种的人工发病方法
US10323253B2 (en) * 2014-12-03 2019-06-18 Monsanto Technology Llc Transgenic plants with enhanced traits
CN106636128A (zh) * 2016-11-15 2017-05-10 内蒙古和盛生态育林有限公司 Cngc2基因和其编码的氨基酸序列在培育植物新品种中的应用
CN107880098B (zh) * 2017-11-08 2020-07-07 中国科学院遗传与发育生物学研究所 大麦抗病蛋白mla10突变体在提高植物对白粉菌抗性中的应用
CN108660141B (zh) * 2018-05-28 2021-08-03 贵州省烟草科学研究院 NtCNGC1基因在烟草抗青枯病中的应用
CN110607385A (zh) * 2019-09-23 2019-12-24 深圳大学 拟南芥叶片锯齿状边缘相关基因功能性分子标记及其应用
CN111394494B (zh) * 2020-02-12 2023-08-25 深圳大学 拟南芥叶片锯齿状边缘相关基因的功能性分子标记的应用
CN112011643B (zh) * 2020-09-30 2022-07-15 河南科技大学 葡萄的qRT-PCR内参基因及其引物与应用
CN116590304B (zh) * 2023-04-06 2024-01-26 东北农业大学 一种洋葱AcCNGC2基因及其应用
CN117430679A (zh) * 2023-07-10 2024-01-23 西北农林科技大学 来源于小麦的广谱抗病相关蛋白及其相关生物材料与应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08505529A (ja) * 1993-01-08 1996-06-18 チバ−ガイギー アクチェンゲゼルシャフト 植物に病気抵抗性をブリーディングする方法
US5629470A (en) * 1995-01-20 1997-05-13 Rutgers, The State University Of New Jersey Transgenic plants and plant cells with enhanced pathogen resistance and related methods
US5986082A (en) * 1996-12-13 1999-11-16 Novartis Ag Altered forms of the NIM1 gene conferring disease resistance in plants

Also Published As

Publication number Publication date
AU6373300A (en) 2001-02-13
JP2003505076A (ja) 2003-02-12
EP1196553A1 (fr) 2002-04-17
WO2001007596A1 (fr) 2001-02-01
MXPA02000781A (es) 2002-08-12

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