CA2377458A1 - Genes de corynebacterium glutamicum pour la biosynthese de l'acide folique et leur utilisation pour la production microbienne d'acide folique - Google Patents
Genes de corynebacterium glutamicum pour la biosynthese de l'acide folique et leur utilisation pour la production microbienne d'acide folique Download PDFInfo
- Publication number
- CA2377458A1 CA2377458A1 CA002377458A CA2377458A CA2377458A1 CA 2377458 A1 CA2377458 A1 CA 2377458A1 CA 002377458 A CA002377458 A CA 002377458A CA 2377458 A CA2377458 A CA 2377458A CA 2377458 A1 CA2377458 A1 CA 2377458A1
- Authority
- CA
- Canada
- Prior art keywords
- polypeptide
- folic acid
- seq
- amino acids
- deletion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 56
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 29
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 29
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 28
- 150000001413 amino acids Chemical class 0.000 claims description 28
- 229960000304 folic acid Drugs 0.000 claims description 28
- 235000019152 folic acid Nutrition 0.000 claims description 28
- 239000011724 folic acid Substances 0.000 claims description 28
- 229920001184 polypeptide Polymers 0.000 claims description 26
- 108090000623 proteins and genes Proteins 0.000 claims description 22
- 108010065780 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase Proteins 0.000 claims description 15
- 238000012217 deletion Methods 0.000 claims description 12
- 230000037430 deletion Effects 0.000 claims description 12
- 238000003780 insertion Methods 0.000 claims description 12
- 230000037431 insertion Effects 0.000 claims description 12
- 238000006467 substitution reaction Methods 0.000 claims description 12
- 108700016256 Dihydropteroate synthases Proteins 0.000 claims description 8
- 108010089355 dihydroneopterin aldolase Proteins 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 7
- 102000036509 GTP Cyclohydrolase Human genes 0.000 claims description 6
- 108010023555 GTP Cyclohydrolase Proteins 0.000 claims description 6
- 108091033319 polynucleotide Proteins 0.000 claims description 6
- 102000040430 polynucleotide Human genes 0.000 claims description 6
- 239000002157 polynucleotide Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims 4
- 238000002955 isolation Methods 0.000 claims 1
- 239000000047 product Substances 0.000 description 10
- 108020004414 DNA Proteins 0.000 description 8
- 244000005700 microbiome Species 0.000 description 8
- 241000186226 Corynebacterium glutamicum Species 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 5
- 101150045875 folP gene Proteins 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 101150011175 folB gene Proteins 0.000 description 4
- 101150075316 folE gene Proteins 0.000 description 4
- 101150095307 folK gene Proteins 0.000 description 4
- 238000010353 genetic engineering Methods 0.000 description 4
- 239000002773 nucleotide Substances 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- 101100227595 Chlamydia pneumoniae folKP gene Proteins 0.000 description 3
- 101100336222 Dictyostelium discoideum gchA gene Proteins 0.000 description 3
- 101100227592 Lactococcus lactis subsp. cremoris (strain MG1363) folKE gene Proteins 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 101100063392 Mycobacterium leprae (strain TN) folP1 gene Proteins 0.000 description 3
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 3
- 101100357018 Trypanosoma brucei brucei RNR2 gene Proteins 0.000 description 3
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 241000186216 Corynebacterium Species 0.000 description 2
- 102100027346 GTP cyclohydrolase 1 Human genes 0.000 description 2
- 241000235648 Pichia Species 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 150000007523 nucleic acids Chemical group 0.000 description 2
- CPNGPNLZQNNVQM-UHFFFAOYSA-N pteridine Chemical group N1=CN=CC2=NC=CN=C21 CPNGPNLZQNNVQM-UHFFFAOYSA-N 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- MSTNYGQPCMXVAQ-RYUDHWBXSA-N (6S)-5,6,7,8-tetrahydrofolic acid Chemical compound C([C@H]1CNC=2N=C(NC(=O)C=2N1)N)NC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 MSTNYGQPCMXVAQ-RYUDHWBXSA-N 0.000 description 1
- ALYNCZNDIQEVRV-UHFFFAOYSA-M 4-aminobenzoate Chemical compound NC1=CC=C(C([O-])=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-M 0.000 description 1
- 102100022524 Alpha-1-antichymotrypsin Human genes 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241001485655 Corynebacterium glutamicum ATCC 13032 Species 0.000 description 1
- 241001465321 Eremothecium Species 0.000 description 1
- 206010016880 Folate deficiency Diseases 0.000 description 1
- 208000010188 Folic Acid Deficiency Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 101000678026 Homo sapiens Alpha-1-antichymotrypsin Proteins 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000186362 Mycobacterium leprae Species 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- KCGUGVQODCQMBI-UHFFFAOYSA-N pyrrolidine-1-carbothioylsulfanyl pyrrolidine-1-carbodithioate Chemical compound C1CCCN1C(=S)SSC(=S)N1CCCC1 KCGUGVQODCQMBI-UHFFFAOYSA-N 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000005460 tetrahydrofolate Substances 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1235—Diphosphotransferases (2.7.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
- C12P17/182—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
L'invention concerne des séquences nucléotidiques de quatre gènes (<i>folE, folP, folB et folK</i>) de <i>Corynebacterium glutamicum</i> servant à la biosynthèse de l'acide folique et leur utilisation pour la production microbienne d'acide folique.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19929363A DE19929363A1 (de) | 1999-06-25 | 1999-06-25 | Gene aus Corynebacterium glutamicum für die Folsäurebiosynthese und ihr Einsatz zur mikrobiellen Herstellung von Folsäure |
| DE19929363.5 | 1999-06-25 | ||
| PCT/EP2000/005864 WO2001000845A1 (fr) | 1999-06-25 | 2000-06-23 | Genes de corynebacterium glutamicum pour la biosynthese de l'acide folique et leur utilisation pour la production microbienne d'acide folique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2377458A1 true CA2377458A1 (fr) | 2001-01-04 |
Family
ID=7912677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002377458A Abandoned CA2377458A1 (fr) | 1999-06-25 | 2000-06-23 | Genes de corynebacterium glutamicum pour la biosynthese de l'acide folique et leur utilisation pour la production microbienne d'acide folique |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP1194565A1 (fr) |
| KR (1) | KR20020026469A (fr) |
| CN (1) | CN1371418A (fr) |
| AU (1) | AU5978200A (fr) |
| CA (1) | CA2377458A1 (fr) |
| DE (1) | DE19929363A1 (fr) |
| WO (1) | WO2001000845A1 (fr) |
| ZA (1) | ZA200200582B (fr) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001002584A1 (fr) | 1999-07-02 | 2001-01-11 | Ajinomoto Co.,Inc. | Adn codant une enzyme ii de pts de sucrose |
| US6958228B2 (en) | 2000-08-02 | 2005-10-25 | Degussa Ag | Nucleotide sequence which code for the metH gene |
| US6942996B2 (en) | 2000-08-02 | 2005-09-13 | Degussa Ag | Isolated polynucleotide from Corynebacterium encoding a homocysteine methyltransferase |
| DE10039043A1 (de) | 2000-08-10 | 2002-02-21 | Degussa | Neue für das luxR-Gen kodierende Nukleotidsequenzen |
| DE10039044A1 (de) | 2000-08-10 | 2002-02-21 | Degussa | Neue für das IysR1-Gen kodierende Nukleotidsequenzen |
| DE10039049A1 (de) | 2000-08-10 | 2002-02-21 | Degussa | Neue für das IysR3-Gen kodierende Nukleotidsequenzen |
| WO2002018429A1 (fr) | 2000-08-26 | 2002-03-07 | Degussa Ag | Sequences nucleotidiques codant pour le gene ccpa2 |
| US6812016B2 (en) | 2000-09-02 | 2004-11-02 | Degussa Ag | Nucleotide sequences which code for the metY gene |
| US6815196B2 (en) | 2000-09-02 | 2004-11-09 | Degussa Ag | Nucleotide sequences encoding o-succinylhomoserine sulfhydrylase |
| AU2001293723A1 (en) | 2000-09-09 | 2002-03-22 | Degussa A.G. | Nucleotide sequences coding for the dep33 gene |
| US6759224B2 (en) | 2000-09-09 | 2004-07-06 | Degussa Ag | Nucleotide sequences which code for the sahH gene |
| DE10045496A1 (de) | 2000-09-14 | 2002-03-28 | Degussa | Neue für das ptsi-Gen kodierende Nukleotidsequenzen |
| DE10055869A1 (de) | 2000-11-10 | 2002-05-29 | Degussa | Neue für das nadA-Gen kodierende Nukleotidsequenzen |
| DE10055870A1 (de) | 2000-11-10 | 2002-05-29 | Degussa | Neue für das nadC-Gen kodierende Nukleotidsequenzen |
| EP1262541A1 (fr) * | 2001-05-28 | 2002-12-04 | Stichting Top-Instituut Voedselwetenschappen | Production de l'acide folique biodisponible |
| US7468262B2 (en) | 2003-05-16 | 2008-12-23 | Ajinomoto Co., Inc. | Polynucleotides encoding useful polypeptides in corynebacterium glutamicum ssp. lactofermentum |
| CN1952114B (zh) * | 2005-10-20 | 2010-04-14 | 浙江爱迪亚营养科技开发有限公司 | 一种谷氨酸棒杆菌及其应用于制备烟酰胺的方法 |
| CN109810991B (zh) * | 2019-03-02 | 2021-11-12 | 昆明理工大学 | 二氢蝶酸合酶基因folP的用途 |
| CN111235169A (zh) * | 2020-02-03 | 2020-06-05 | 昆明理工大学 | 一种GTP环化水解酶I基因folE及应用 |
| CN112852844A (zh) * | 2021-03-05 | 2021-05-28 | 昆明理工大学 | 羟甲基二氢蝶呤焦磷酸激酶基因folK的用途 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5968788A (en) * | 1995-08-28 | 1999-10-19 | Toray Industries, Inc. | Method for producing folic acid |
-
1999
- 1999-06-25 DE DE19929363A patent/DE19929363A1/de not_active Withdrawn
-
2000
- 2000-06-23 KR KR1020017016565A patent/KR20020026469A/ko not_active Withdrawn
- 2000-06-23 CA CA002377458A patent/CA2377458A1/fr not_active Abandoned
- 2000-06-23 WO PCT/EP2000/005864 patent/WO2001000845A1/fr not_active Ceased
- 2000-06-23 EP EP00945815A patent/EP1194565A1/fr not_active Withdrawn
- 2000-06-23 CN CN00812014A patent/CN1371418A/zh active Pending
- 2000-06-23 AU AU59782/00A patent/AU5978200A/en not_active Abandoned
-
2002
- 2002-01-23 ZA ZA200200582A patent/ZA200200582B/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP1194565A1 (fr) | 2002-04-10 |
| DE19929363A1 (de) | 2000-12-28 |
| AU5978200A (en) | 2001-01-31 |
| CN1371418A (zh) | 2002-09-25 |
| ZA200200582B (en) | 2003-03-26 |
| KR20020026469A (ko) | 2002-04-10 |
| WO2001000845A1 (fr) | 2001-01-04 |
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