CA2331688C - Detection of a target in a sample - Google Patents
Detection of a target in a sample Download PDFInfo
- Publication number
- CA2331688C CA2331688C CA002331688A CA2331688A CA2331688C CA 2331688 C CA2331688 C CA 2331688C CA 002331688 A CA002331688 A CA 002331688A CA 2331688 A CA2331688 A CA 2331688A CA 2331688 C CA2331688 C CA 2331688C
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- electrodes
- target
- assay
- targets
- sample
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Detergent Compositions (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2641613A CA2641613C (en) | 1998-05-04 | 1999-05-04 | Detection of a target in a sample |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL124322 | 1998-05-04 | ||
| IL12432298A IL124322A (en) | 1998-05-04 | 1998-05-04 | Detection of an entity in a sample |
| PCT/IL1999/000232 WO1999057550A1 (en) | 1998-05-04 | 1999-05-04 | Detection of a target in a sample |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2641613A Division CA2641613C (en) | 1998-05-04 | 1999-05-04 | Detection of a target in a sample |
Publications (2)
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| CA2331688A1 CA2331688A1 (en) | 1999-11-11 |
| CA2331688C true CA2331688C (en) | 2008-12-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2820735A Abandoned CA2820735A1 (en) | 1998-05-04 | 1999-05-04 | Detection of a target in a sample |
| CA002331688A Expired - Fee Related CA2331688C (en) | 1998-05-04 | 1999-05-04 | Detection of a target in a sample |
| CA2641613A Expired - Fee Related CA2641613C (en) | 1998-05-04 | 1999-05-04 | Detection of a target in a sample |
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| CA2820735A Abandoned CA2820735A1 (en) | 1998-05-04 | 1999-05-04 | Detection of a target in a sample |
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| Application Number | Title | Priority Date | Filing Date |
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| CA2641613A Expired - Fee Related CA2641613C (en) | 1998-05-04 | 1999-05-04 | Detection of a target in a sample |
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| Country | Link |
|---|---|
| US (2) | US20120309106A1 (enExample) |
| EP (1) | EP1075656B1 (enExample) |
| JP (1) | JP4618886B2 (enExample) |
| AT (1) | ATE515693T1 (enExample) |
| AU (1) | AU3625999A (enExample) |
| CA (3) | CA2820735A1 (enExample) |
| IL (1) | IL124322A (enExample) |
| WO (1) | WO1999057550A1 (enExample) |
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| IL121312A (en) | 1997-07-14 | 2001-09-13 | Technion Res & Dev Foundation | Microelectronic components, their manufacture and electronic networks containing them |
| IL126776A (en) | 1998-10-27 | 2001-04-30 | Technion Res & Dev Foundation | A method of investing gold |
| AU773978B2 (en) * | 1999-04-07 | 2004-06-10 | Dennis Michael Connolly | High resolution DNA detection methods and devices |
| US7364920B2 (en) | 1999-10-27 | 2008-04-29 | Technion Research And Development Foundation Ltd. | Method for gold deposition |
| DE10113550A1 (de) * | 2001-03-20 | 2002-10-02 | Infineon Technologies Ag | Verfahren zum Erfassen von makromolekularen Biopolymeren mittels einer Elektrodenanordnung |
| US6824974B2 (en) | 2001-06-11 | 2004-11-30 | Genorx, Inc. | Electronic detection of biological molecules using thin layers |
| US20040248282A1 (en) * | 2001-06-11 | 2004-12-09 | Pisharody Sobha M. | Electronic detection of biological molecules using thin layers |
| US20040023253A1 (en) * | 2001-06-11 | 2004-02-05 | Sandeep Kunwar | Device structure for closely spaced electrodes |
| CN100344960C (zh) * | 2001-12-17 | 2007-10-24 | 清华大学 | 刺激动物细胞并记录其生理信号的装置及其生产使用方法 |
| US20030186245A1 (en) * | 2002-03-28 | 2003-10-02 | Roitman Daniel B. | Biomolecular sensors and detection methods utilizing photoinduced charge separation |
| DE10228260A1 (de) * | 2002-06-25 | 2004-01-22 | Bayer Ag | Methode und Vorrichtung zum impedimetrischen Nachweis eines oder mehrerer Analyten in einer Probe |
| US20040166520A1 (en) * | 2003-01-03 | 2004-08-26 | Connolly D. Michael | Identifying items with nucleic acid taggants |
| DE10322912A1 (de) * | 2003-05-21 | 2004-12-16 | Bayer Technology Services Gmbh | Verfahren zum Nachweis von Nukleinsäuren |
| DE10327683A1 (de) * | 2003-06-20 | 2005-01-20 | Bayer Technology Services Gmbh | Verfahren und Vorrichtung zum quantitativen elektrischen Nachweis von Analyten |
| DE10330717A1 (de) * | 2003-07-03 | 2005-02-10 | Friedrich-Schiller-Universität Jena | Vorrichtung und Verfahren zum Nachweis von Analyt |
| US20050079598A1 (en) * | 2003-10-08 | 2005-04-14 | Davis Randall W. | Apparatus and method for identification of biomolecules, in particular nucleic acid sequences, proteins, and antigens and antibodies |
| WO2005045409A1 (ja) * | 2003-11-07 | 2005-05-19 | Osaka Prefecture | 電気抵抗型検出センサおよび検出方法 |
| US8227380B2 (en) * | 2004-04-29 | 2012-07-24 | Marc Ramael | Method and kit for detecting components in a sample |
| EP1749108A2 (en) * | 2004-05-28 | 2007-02-07 | Nanogen, Inc. | Nanoscale electronic detection system and methods for their manufacture |
| JP4873463B2 (ja) * | 2006-06-30 | 2012-02-08 | 独立行政法人産業技術総合研究所 | ナノギャップ電極を有する検出素子及びこれを用いた検出方法 |
| US9395352B2 (en) | 2007-04-06 | 2016-07-19 | Arizona Board Of Regents On Behalf Of Arizona State University | Devices and methods for target molecule characterization |
| WO2010042514A1 (en) | 2008-10-06 | 2010-04-15 | Arizona Board Of Regents | Nanopore and carbon nanotube based dna sequencer and a serial recognition elements |
| US9140682B2 (en) | 2010-02-02 | 2015-09-22 | Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Acting For And On Behalf Of Arizona State University | Controlled tunnel gap device for sequencing polymers |
| WO2014059144A1 (en) | 2012-10-10 | 2014-04-17 | Arizona Board Of Regents Acting For And On Behalf Of Arizona State University | Systems and devices for molecule sensing and method of manufacturing thereof |
| EP3408219B1 (en) | 2016-01-28 | 2022-08-17 | Roswell Biotechnologies, Inc | Massively parallel dna sequencing apparatus |
| US11624725B2 (en) * | 2016-01-28 | 2023-04-11 | Roswell Blotechnologies, Inc. | Methods and apparatus for measuring analytes using polymerase in large scale molecular electronics sensor arrays |
| EP3882616A1 (en) | 2016-02-09 | 2021-09-22 | Roswell Biotechnologies, Inc | Electronic label-free dna and genome sequencing |
| CA3023256A1 (en) * | 2016-05-18 | 2017-11-23 | Integrated Nano-Technologies, Inc. | Method for detection of a pcr product |
| WO2018136148A1 (en) | 2017-01-19 | 2018-07-26 | Roswell Biotechnologies, Inc. | Solid state sequencing devices comprising two dimensional layer materials |
| EP3615685B1 (en) | 2017-04-25 | 2025-02-19 | Roswell Biotechnologies, Inc | Enzymatic circuits for molecular sensors |
| US10508296B2 (en) | 2017-04-25 | 2019-12-17 | Roswell Biotechnologies, Inc. | Enzymatic circuits for molecular sensors |
| EP3622086A4 (en) | 2017-05-09 | 2021-04-21 | Roswell Biotechnologies, Inc | LINK PROBE CIRCUITS FOR MOLECULAR SENSORS |
| CN111373049A (zh) | 2017-08-30 | 2020-07-03 | 罗斯威尔生命技术公司 | 用于dna数据存储的进行性酶分子电子传感器 |
| CN111373051A (zh) | 2017-10-10 | 2020-07-03 | 罗斯威尔生命技术公司 | 用于无扩增dna数据存储的方法、装置和系统 |
| US20210373014A1 (en) * | 2017-10-23 | 2021-12-02 | The General Hospital Corporation | An integrated microfluidic electrode array system for enzyme-linked immuno-sorbent assay (easy-elisa) for point-of-care detection of molecular and cellular biomarkers |
| KR20210045361A (ko) | 2018-05-17 | 2021-04-26 | 레커그니션 어낼러틱스 엘엘씨 | 효소 활성의 직접 전기적 측정을 위한 장치, 시스템 및 방법 |
| JP2020041909A (ja) * | 2018-09-11 | 2020-03-19 | 国立大学法人九州大学 | 内分泌攪乱物質等の定量方法及び定量装置 |
| BR112021014800A2 (pt) | 2019-01-30 | 2022-01-04 | Univ Arizona State | Circuitos bioeletrônicos, sistemas e métodos para preparação dos mesmos |
| CN113994013A (zh) | 2019-04-12 | 2022-01-28 | 罗斯威尔生命技术公司 | 用于分子电子学传感器的多环芳族桥 |
| US20230002819A1 (en) * | 2019-08-22 | 2023-01-05 | Universal Sequencing Technology Corporation | Methods for Biomolecular Sensing and Detection |
| US12146852B2 (en) | 2019-09-06 | 2024-11-19 | Roswell Biotechnologies, Inc. | Methods of fabricating nanoscale structures usable in molecular sensors and other devices |
| WO2022140707A2 (en) | 2020-12-25 | 2022-06-30 | Nagy Aurangzeb Nafees | Detection of target nucleic acid molecules |
| JP7555166B1 (ja) * | 2024-07-04 | 2024-09-24 | 株式会社イムノセンス | 電気化学式免疫センサー |
Family Cites Families (10)
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|---|---|---|---|---|
| US5137827A (en) * | 1986-03-25 | 1992-08-11 | Midwest Research Technologies, Inc. | Diagnostic element for electrical detection of a binding reaction |
| US4794089A (en) * | 1986-03-25 | 1988-12-27 | Midwest Research Microscopy, Inc. | Method for electronic detection of a binding reaction |
| US5108889A (en) * | 1988-10-12 | 1992-04-28 | Thorne, Smith, Astill Technologies, Inc. | Assay for determining analyte using mercury release followed by detection via interaction with aluminum |
| AU4647589A (en) * | 1988-11-10 | 1990-05-28 | Midwest Research Technologies, Inc. | Method for electrical detection of a binding reaction |
| CA2034266A1 (en) * | 1990-02-27 | 1991-08-28 | Paul A. D'orazio | Thick film reagent spreading and reagent immobilization layer |
| IL103674A0 (en) * | 1991-11-19 | 1993-04-04 | Houston Advanced Res Center | Method and apparatus for molecule detection |
| US5846708A (en) * | 1991-11-19 | 1998-12-08 | Massachusetts Institiute Of Technology | Optical and electrical methods and apparatus for molecule detection |
| WO1997021094A1 (en) * | 1995-12-01 | 1997-06-12 | Innogenetics N.V. | Impedimetric detection system and method of production thereof |
| US6506895B2 (en) * | 1997-08-15 | 2003-01-14 | Surmodics, Inc. | Photoactivatable nucleic acids |
| IL126776A (en) * | 1998-10-27 | 2001-04-30 | Technion Res & Dev Foundation | A method of investing gold |
-
1998
- 1998-05-04 IL IL12432298A patent/IL124322A/en not_active IP Right Cessation
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1999
- 1999-05-04 CA CA2820735A patent/CA2820735A1/en not_active Abandoned
- 1999-05-04 WO PCT/IL1999/000232 patent/WO1999057550A1/en not_active Ceased
- 1999-05-04 JP JP2000547465A patent/JP4618886B2/ja not_active Expired - Fee Related
- 1999-05-04 AU AU36259/99A patent/AU3625999A/en not_active Abandoned
- 1999-05-04 EP EP99918252A patent/EP1075656B1/en not_active Expired - Lifetime
- 1999-05-04 AT AT99918252T patent/ATE515693T1/de not_active IP Right Cessation
- 1999-05-04 CA CA002331688A patent/CA2331688C/en not_active Expired - Fee Related
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2011
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2016
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Also Published As
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|---|---|
| AU3625999A (en) | 1999-11-23 |
| IL124322A0 (en) | 1998-12-06 |
| CA2331688A1 (en) | 1999-11-11 |
| US20170121761A1 (en) | 2017-05-04 |
| WO1999057550A1 (en) | 1999-11-11 |
| JP4618886B2 (ja) | 2011-01-26 |
| ATE515693T1 (de) | 2011-07-15 |
| CA2641613C (en) | 2013-06-25 |
| IL124322A (en) | 2002-05-23 |
| JP2002522748A (ja) | 2002-07-23 |
| CA2641613A1 (en) | 1999-11-11 |
| CA2820735A1 (en) | 1999-11-11 |
| US20120309106A1 (en) | 2012-12-06 |
| EP1075656A1 (en) | 2001-02-14 |
| EP1075656B1 (en) | 2011-07-06 |
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