CA2315296A1 - Embouts en phase solide et utilisations correspondantes - Google Patents

Embouts en phase solide et utilisations correspondantes Download PDF

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Publication number
CA2315296A1
CA2315296A1 CA002315296A CA2315296A CA2315296A1 CA 2315296 A1 CA2315296 A1 CA 2315296A1 CA 002315296 A CA002315296 A CA 002315296A CA 2315296 A CA2315296 A CA 2315296A CA 2315296 A1 CA2315296 A1 CA 2315296A1
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CA
Canada
Prior art keywords
tip
solid
tip structure
biomolecule
chemical layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002315296A
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English (en)
Inventor
Lori K. Garrison
John C. Tabone
Jeffrey Van Ness
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Qiagen Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Genomics Inc filed Critical Qiagen Genomics Inc
Publication of CA2315296A1 publication Critical patent/CA2315296A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00387—Applications using probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/0047—Pins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/0047—Pins
    • B01J2219/00472—Replaceable crowns
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00504—Pins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00504—Pins
    • B01J2219/00506—Pins with removable crowns
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00725—Peptides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

Les techniques de dosage en phase solide ont apporté une approche efficace à l'analyse des biomolécules dans le diagnostic médical et la recherche de base. Des procédés d'hybridation d'acides nucléiques en phase solide ont par exemple été appliqués à l'analyse des polymorphismes génétiques, au diagnostic des maladies génétiques, au diagnostic du cancer, à la détection des pathogènes viraux et microbiens, au criblage des clones et au classement des fragments génomiques. Cette invention présente un nouvel embout de collecte d'échantillons en phase solide qui permet d'améliorer les techniques de synthèse ou de détection des biomolécules. Ces embouts peuvent être utilisés pour mettre au point des systèmes de collecte d'échantillons, qui, à leur tour, peuvent être combinés pour former des réseaux matriciels de systèmes de collecte d'échantillons en phase solide, utiles dans des processus automatisés. Ces embouts peuvent être reliés à une tige de support sollicitée par ressort et ils sont également recouverts d'une couche chimique à laquelle une biomolécule peut se fixer.
CA002315296A 1997-12-31 1998-12-30 Embouts en phase solide et utilisations correspondantes Abandoned CA2315296A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7029097P 1997-12-31 1997-12-31
US60/070,290 1997-12-31
PCT/US1998/027850 WO1999034214A1 (fr) 1997-12-31 1998-12-30 Embouts en phase solide et utilisations correspondantes

Publications (1)

Publication Number Publication Date
CA2315296A1 true CA2315296A1 (fr) 1999-07-08

Family

ID=22094390

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002315296A Abandoned CA2315296A1 (fr) 1997-12-31 1998-12-30 Embouts en phase solide et utilisations correspondantes

Country Status (8)

Country Link
EP (1) EP1040352A1 (fr)
JP (1) JP2002500362A (fr)
CN (1) CN1284168A (fr)
AU (1) AU2098999A (fr)
BR (1) BR9814604A (fr)
CA (1) CA2315296A1 (fr)
HU (1) HUP0100697A2 (fr)
WO (1) WO1999034214A1 (fr)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1821700A (en) * 1998-11-18 2000-06-05 Orchid Biosciences, Inc. One-step nucleic acid dipstick device with movable membrane
NL1012394C2 (nl) * 1999-06-21 2000-12-22 Inst F R Biotechnologie Werkwijze en inrichting voor het gelijktijdig en onafhankelijk van elkaar bemonsteren van ingevroren cultures van micro-organismen en/of eukaryote cellen.
ATE297252T1 (de) * 1999-08-17 2005-06-15 Ttp Labtech Ltd Probeentnahme- und -abgabegerät mit kolben und um den kolben herum gearbeitetes gehäuse
WO2001019518A1 (fr) * 1999-09-13 2001-03-22 Aclara Biosciences, Inc. Dispositif et procede d'application de gouttelettes a liberation par inertie et a chargement par tension superficielle
JP2001136964A (ja) * 1999-11-12 2001-05-22 Shimadzu Corp Ｐｃｒプレート及びｄｎａチップの作製方法
AU7166201A (en) * 2000-06-28 2002-01-08 Illumina Inc Composite arrays utilizing microspheres with a hybridization chamber
JP2003091787A (ja) * 2001-09-17 2003-03-28 Riken Keiki Co Ltd 可搬式ガス警報器
US7142987B2 (en) * 2001-11-07 2006-11-28 Genvault Corporation Apparatus, system, and method of archival and retrieval of samples
US7584240B2 (en) 2001-11-07 2009-09-01 Genvault Corporation Automated biological sample archive for storage, retrieval and analysis of large numbers of samples for remote clients
US7718442B2 (en) 2002-11-22 2010-05-18 Genvault Corporation Sealed sample storage element system and method
CA2567720A1 (fr) 2004-05-24 2005-12-08 Genvault Corporation Stockage proteique stable et stockage d'acides nucleiques stable sous forme recuperable
WO2008154225A2 (fr) 2007-06-06 2008-12-18 Bayer Healthcare Llc Système de microdépôt pour biocapteur
JP5659024B2 (ja) * 2008-03-12 2015-01-28 セレクトリコン アーベー マルチウェルプレートにおいてチップを整列させる装置及び方法
CN102177237B (zh) 2008-09-12 2013-10-30 金沃特公司 用于贮存和稳定生物分子的基质和介质
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
EP2812344A4 (fr) 2012-02-07 2015-10-28 Vibrant Holdings Llc Substrats, réseaux de peptides et procédés
US10006909B2 (en) 2012-09-28 2018-06-26 Vibrant Holdings, Llc Methods, systems, and arrays for biomolecular analysis
WO2014052989A2 (fr) * 2012-09-28 2014-04-03 Vibrant Holdings, Llc Procédés, systèmes et arrangements d'analyse biomoléculaire
US10286376B2 (en) 2012-11-14 2019-05-14 Vibrant Holdings, Llc Substrates, systems, and methods for array synthesis and biomolecular analysis
JP5597731B2 (ja) * 2013-01-04 2014-10-01 あおい精機株式会社 検査前処理装置
EP4030167A1 (fr) 2013-02-15 2022-07-20 Vibrant Holdings, LLC Procédés et compositions de détection de l'électrochimioluminescence amplifiée
WO2018218250A2 (fr) 2017-05-26 2018-11-29 Vibrant Holdings, Llc Composés photoactifs et procédés de détection et de séquençage de biomolécules
CN114761116A (zh) * 2019-12-03 2022-07-15 深圳华大基因科技有限公司 寡核苷酸的浸没合成
CN112162027B (zh) * 2020-09-21 2023-08-18 上海市计量测试技术研究院 一种基于三嵌段探针的电化学传感器及其在检测转基因双链rna中的应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2197720A (en) * 1986-11-20 1988-05-25 Nat Res Dev Immobilisation of polynucleotides
EP0498801B1 (fr) * 1989-09-15 1998-11-04 Chiron Mimotopes Pty. Ltd. Surface solide pour la synthese de peptides
SE467308B (sv) * 1990-10-22 1992-06-29 Berol Nobel Ab Fast yta belagd med ett hydrofilt ytterskikt med kovalent bundna biopolymerer, saett att framstaella en saadan yta och ett konjugat daerfoer
CA2148591A1 (fr) * 1992-11-06 1994-05-26 H. Mario Geysen Support pour la synthese de polymeres modulaires
WO1998014271A1 (fr) * 1996-10-04 1998-04-09 Gordon, Steven, J. Elements remplaçables destines a un dispositif parallele sequentiel de synthese de biopolymeres
US6365349B1 (en) * 1997-07-22 2002-04-02 Qiagen Genomics, Inc. Apparatus and methods for arraying solution onto a solid support
NZ501968A (en) * 1997-07-22 2001-09-28 Qiagen Genomics Inc Polyethylenimine-based biomolecule arrays
DE19742227A1 (de) * 1997-09-25 1999-04-01 Juergen Prof Dipl Phys Wolfrum Verfahren zum Sequenzieren eines einzelnen DNA-Moleküls

Also Published As

Publication number Publication date
WO1999034214A1 (fr) 1999-07-08
BR9814604A (pt) 2000-10-17
JP2002500362A (ja) 2002-01-08
EP1040352A1 (fr) 2000-10-04
HUP0100697A2 (hu) 2001-06-28
CN1284168A (zh) 2001-02-14
AU2098999A (en) 1999-07-19

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