CA2303123C - Method for detecting antibodies in a sample - Google Patents
Method for detecting antibodies in a sample Download PDFInfo
- Publication number
- CA2303123C CA2303123C CA002303123A CA2303123A CA2303123C CA 2303123 C CA2303123 C CA 2303123C CA 002303123 A CA002303123 A CA 002303123A CA 2303123 A CA2303123 A CA 2303123A CA 2303123 C CA2303123 C CA 2303123C
- Authority
- CA
- Canada
- Prior art keywords
- ligand
- target antigen
- antibodies
- coupled
- subject
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 239000000427 antigen Substances 0.000 claims abstract description 76
- 108091007433 antigens Proteins 0.000 claims abstract description 75
- 102000036639 antigens Human genes 0.000 claims abstract description 75
- 238000012360 testing method Methods 0.000 claims abstract description 50
- 239000007790 solid phase Substances 0.000 claims abstract description 26
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 241000711549 Hepacivirus C Species 0.000 claims abstract description 8
- 238000011534 incubation Methods 0.000 claims abstract description 8
- 239000003446 ligand Substances 0.000 claims description 43
- 239000003153 chemical reaction reagent Substances 0.000 claims description 35
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- 108060003951 Immunoglobulin Proteins 0.000 claims description 17
- 108020001507 fusion proteins Proteins 0.000 claims description 17
- 102000037865 fusion proteins Human genes 0.000 claims description 17
- 102000018358 immunoglobulin Human genes 0.000 claims description 17
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- 108010012715 Superoxide dismutase Proteins 0.000 claims description 11
- 241000700605 Viruses Species 0.000 claims description 7
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
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- 235000001014 amino acid Nutrition 0.000 description 2
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- FQQREHKSHAYSMG-UHFFFAOYSA-N 1,2-dimethylacridine Chemical compound C1=CC=CC2=CC3=C(C)C(C)=CC=C3N=C21 FQQREHKSHAYSMG-UHFFFAOYSA-N 0.000 description 1
- DJQYYYCQOZMCRC-UHFFFAOYSA-N 2-aminopropane-1,3-dithiol Chemical compound SCC(N)CS DJQYYYCQOZMCRC-UHFFFAOYSA-N 0.000 description 1
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- 241000233866 Fungi Species 0.000 description 1
- 208000005176 Hepatitis C Diseases 0.000 description 1
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- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
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- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 108010076039 Polyproteins Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 208000019802 Sexually transmitted disease Diseases 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000002820 assay format Methods 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
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- 238000007796 conventional method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
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- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
- G01N33/5767—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis non-A, non-B hepatitis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/82—Hepatitis associated antigens and antibodies
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Communicable Diseases (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US5970397P | 1997-09-22 | 1997-09-22 | |
| US60/059,703 | 1997-09-22 | ||
| US8392198P | 1998-05-01 | 1998-05-01 | |
| US60/083,921 | 1998-05-01 | ||
| PCT/US1998/019693 WO1999015898A1 (en) | 1997-09-22 | 1998-09-22 | Method for detecting antibodies in a sample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2303123A1 CA2303123A1 (en) | 1999-04-01 |
| CA2303123C true CA2303123C (en) | 2006-04-11 |
Family
ID=26739077
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002303123A Expired - Lifetime CA2303123C (en) | 1997-09-22 | 1998-09-22 | Method for detecting antibodies in a sample |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US6391540B1 (enExample) |
| EP (1) | EP1021719B1 (enExample) |
| JP (1) | JP4124961B2 (enExample) |
| AT (1) | ATE354800T1 (enExample) |
| AU (1) | AU9497998A (enExample) |
| CA (1) | CA2303123C (enExample) |
| CY (1) | CY1107621T1 (enExample) |
| DE (1) | DE69837155T2 (enExample) |
| DK (1) | DK1021719T3 (enExample) |
| ES (1) | ES2281934T3 (enExample) |
| PT (1) | PT1021719E (enExample) |
| WO (1) | WO1999015898A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1295126B1 (en) * | 1999-07-28 | 2007-01-24 | Novartis Vaccines and Diagnostics, Inc. | Hepatitis c viral antigen immunoassay detection systems |
| US6815160B1 (en) | 1999-07-28 | 2004-11-09 | Chiron Corporation | Hepatitis C viral antigen immunoassay detection systems |
| US6995299B2 (en) * | 1999-11-02 | 2006-02-07 | University Of Connecticut | Propagation of human hepatocytes in non-human animals |
| DE10006432A1 (de) * | 2000-02-14 | 2001-08-16 | Ganzimmun Inst Fuer Ganzheitli | Verfahren zum Nachweis von Helicobacter pylori in Stuhl- und Speichelproben |
| BRPI0111731C1 (pt) * | 2000-06-15 | 2021-07-27 | Chiron Corp | suporte sólido de imunoensaio, métodos de detecção de infecção pelo vírus da hepatite c (hcv) em uma amostra biológica, e de produção de um suporte sólido de imunoensaio, e, kit de teste de imunodiagnóstico |
| GB0325483D0 (en) * | 2003-10-31 | 2003-12-03 | Plasmacute As | Assay |
| JP2007532905A (ja) * | 2004-04-15 | 2007-11-15 | アライド・バイオテック・インコーポレーテッド | ウイルス感染の検出のための方法と装置 |
| JP5414667B2 (ja) | 2007-05-08 | 2014-02-12 | エフ．ホフマン−ラ ロシュ アーゲー | 特異的免疫グロブリンクラスｇ抗体の検出方法 |
| CN105486856A (zh) * | 2015-12-24 | 2016-04-13 | 泰州泽成生物技术有限公司 | 一种定性检测丙型肝炎抗体的试剂盒及其制备方法和应用 |
| CN107167584B (zh) * | 2017-05-27 | 2020-03-03 | 北京热景生物技术股份有限公司 | 化学发光免疫分析仪 |
| JP7198932B2 (ja) | 2018-09-18 | 2023-01-04 | シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレイテッド | ジカウイルスイムノアッセイの方法および試薬 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2556840B1 (fr) | 1983-12-16 | 1987-12-24 | Immunotech Sa | Procede de dosage in vitro des immunoglobulines specifiques d'un ou plusieurs allergenes et reactifs et moyens pour la mise en oeuvre de ce procede |
| US4737453A (en) * | 1984-12-12 | 1988-04-12 | Immunomedics, Inc. | Sandwich immunoassay utilizing a separation specific binding substance |
| EP0341439B1 (en) | 1988-05-11 | 1993-08-04 | Abbott Laboratories | Method for increasing specificity in competitive immunoassays |
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-
1998
- 1998-09-22 CA CA002303123A patent/CA2303123C/en not_active Expired - Lifetime
- 1998-09-22 DE DE69837155T patent/DE69837155T2/de not_active Expired - Lifetime
- 1998-09-22 AU AU94979/98A patent/AU9497998A/en not_active Abandoned
- 1998-09-22 EP EP98948398A patent/EP1021719B1/en not_active Expired - Lifetime
- 1998-09-22 AT AT98948398T patent/ATE354800T1/de active
- 1998-09-22 US US09/158,301 patent/US6391540B1/en not_active Expired - Lifetime
- 1998-09-22 PT PT98948398T patent/PT1021719E/pt unknown
- 1998-09-22 JP JP2000513145A patent/JP4124961B2/ja not_active Expired - Lifetime
- 1998-09-22 WO PCT/US1998/019693 patent/WO1999015898A1/en not_active Ceased
- 1998-09-22 ES ES98948398T patent/ES2281934T3/es not_active Expired - Lifetime
- 1998-09-22 DK DK98948398T patent/DK1021719T3/da active
-
2007
- 2007-04-26 CY CY20071100557T patent/CY1107621T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE69837155T2 (de) | 2007-06-21 |
| CA2303123A1 (en) | 1999-04-01 |
| US6391540B1 (en) | 2002-05-21 |
| DE69837155D1 (de) | 2007-04-05 |
| JP4124961B2 (ja) | 2008-07-23 |
| DK1021719T3 (da) | 2007-06-18 |
| EP1021719A1 (en) | 2000-07-26 |
| ES2281934T3 (es) | 2007-10-01 |
| CY1107621T1 (el) | 2013-03-13 |
| ATE354800T1 (de) | 2007-03-15 |
| WO1999015898A1 (en) | 1999-04-01 |
| PT1021719E (pt) | 2007-03-30 |
| AU9497998A (en) | 1999-04-12 |
| JP2001517797A (ja) | 2001-10-09 |
| EP1021719B1 (en) | 2007-02-21 |
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