CA2291803C - Methods for coexpression of more than one gene using at least one internal ribosome entry site (ires) - Google Patents

Methods for coexpression of more than one gene using at least one internal ribosome entry site (ires) Download PDF

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Publication number
CA2291803C
CA2291803C CA002291803A CA2291803A CA2291803C CA 2291803 C CA2291803 C CA 2291803C CA 002291803 A CA002291803 A CA 002291803A CA 2291803 A CA2291803 A CA 2291803A CA 2291803 C CA2291803 C CA 2291803C
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Prior art keywords
gene
ires
plant
iresmp
expressible
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Expired - Fee Related
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CA002291803A
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English (en)
French (fr)
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CA2291803A1 (en
Inventor
Joseph Atabekov
Timo Korpela
Yurii Dorokhov
Peter Ivanov
Nina Rodionova
Olga Karpova
Maxim Skulachev
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Control Of Motors That Do Not Use Commutators (AREA)
  • Epoxy Resins (AREA)
  • Earth Drilling (AREA)
CA002291803A 1997-05-30 1998-05-29 Methods for coexpression of more than one gene using at least one internal ribosome entry site (ires) Expired - Fee Related CA2291803C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FI972293 1997-05-30
FI972293A FI972293L (fi) 1997-05-30 1997-05-30 Useamman kuin yhden geenin yhteisekspressiomenetelmät
PCT/FI1998/000457 WO1998054342A1 (en) 1997-05-30 1998-05-29 Methods for coexpression of more than one gene using at least one internal ribosome entry site (ires)

Publications (2)

Publication Number Publication Date
CA2291803A1 CA2291803A1 (en) 1998-12-03
CA2291803C true CA2291803C (en) 2009-12-15

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CA002291803A Expired - Fee Related CA2291803C (en) 1997-05-30 1998-05-29 Methods for coexpression of more than one gene using at least one internal ribosome entry site (ires)

Country Status (9)

Country Link
US (1) US6376745B1 (enExample)
EP (1) EP1017834B1 (enExample)
JP (1) JP2002514086A (enExample)
AT (1) ATE369433T1 (enExample)
AU (1) AU7533998A (enExample)
CA (1) CA2291803C (enExample)
DE (1) DE69838222T2 (enExample)
FI (1) FI972293L (enExample)
WO (1) WO1998054342A1 (enExample)

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US6933378B2 (en) * 1997-05-30 2005-08-23 Joseph Atabekov Methods for coexpression of more than one gene in eukaryotic cells
US7227057B2 (en) 1997-06-03 2007-06-05 Chromatin, Inc. Plant centromere compositions
US7235716B2 (en) 1997-06-03 2007-06-26 Chromatin, Inc. Plant centromere compositions
US7193128B2 (en) 1997-06-03 2007-03-20 Chromatin, Inc. Methods for generating or increasing revenues from crops
US7119250B2 (en) 1997-06-03 2006-10-10 The University Of Chicago Plant centromere compositions
US7989202B1 (en) 1999-03-18 2011-08-02 The University Of Chicago Plant centromere compositions
EP1196614A1 (en) * 1999-06-24 2002-04-17 Metabolix, Inc. Plant multi-gene expression constructs
AU2001242360A1 (en) * 2000-02-10 2001-08-20 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Plant internal ribosome entry segment
GB0019745D0 (en) * 2000-08-10 2000-09-27 Univ Surrey Gene expression element
DE10049587A1 (de) * 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
WO2002040690A2 (en) 2000-11-17 2002-05-23 Metabolix, Inc. Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways
DE10061150A1 (de) * 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
US20030049228A1 (en) * 2001-01-09 2003-03-13 Simon Santa-Cruz Expression of foreign genes from plant virus vectors
DE10102389A1 (de) 2001-01-19 2002-08-01 Icon Genetics Ag Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen
DE10109354A1 (de) * 2001-02-27 2002-09-05 Icon Genetics Ag Rekombinante virale Schaltersysteme
DE10114209A1 (de) 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
DE10115507A1 (de) 2001-03-29 2002-10-10 Icon Genetics Ag Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus
MXPA02012475A (es) 2001-04-17 2004-02-26 Icon Genetics Inc Captura de genes activados ires en plantas.
DE10121283B4 (de) 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen
CN1533438A (zh) * 2001-06-08 2004-09-29 �����Ŵ��Ƽ���˾ 在植物生产蛋白质的方法
DE10132780A1 (de) 2001-07-06 2003-01-16 Icon Genetics Ag Plastidäre Genexpression über autonom replizierende Vektoren
DE10143237A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
DE10143238A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente
US20040033603A1 (en) * 2002-08-19 2004-02-19 Lin Zhang Biotinylation of proteins
DE10303937A1 (de) * 2003-01-31 2004-08-12 Icon Genetics Ag Pflanzentransformation mit Assemblierung einer gewünschten Sequenz in Vivo
ATE426672T1 (de) * 2003-01-31 2009-04-15 Icon Genetics Gmbh Pflanzentransformation mit in-vivo-zusammenbau einer eigenschaft
WO2005049839A2 (en) 2003-11-10 2005-06-02 Icon Genetics Ag Rna virus-derived plant expression system
CA2557644C (en) * 2004-02-23 2016-05-24 Chromatin, Inc. Plants modified with mini-chromosomes
EP1616959A1 (en) 2004-07-07 2006-01-18 Icon Genetics AG Biological safe transient protein expression in plants
US8222028B2 (en) * 2005-09-08 2012-07-17 Chromatin, Inc. Plants modified with mini-chromosomes
US20100003686A1 (en) 2006-10-23 2010-01-07 Research Organization Of Information And Systems Process for producing tetracycline inducible gene expressing cell line and conditional gene knockout cell line, and uses thereof
US8614089B2 (en) * 2007-03-15 2013-12-24 Chromatin, Inc. Centromere sequences and minichromosomes
EP2456894A4 (en) 2009-07-23 2013-09-25 Chromatin Inc CENTROMER SEQUENCES AND SORGHUM MINI-CHROMOSOMES
US8945876B2 (en) 2011-11-23 2015-02-03 University Of Hawaii Auto-processing domains for polypeptide expression
DE102017103383A1 (de) 2017-02-20 2018-08-23 aReNA-Bio GbR (vertretungsberechtigter Gesellschafter: Dr. Heribert Bohlen, 50733 Köln) System und Verfahren zur Zelltyp-spezifischen Translation von RNA-Molekülen in Eukaryoten
CN121195070A (zh) 2023-04-03 2025-12-23 斯威夫特制药有限责任公司 用于在植物中高效异源基因表达的反式互补载体系统

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Publication number Priority date Publication date Assignee Title
US5977438A (en) 1988-02-26 1999-11-02 Biosource Technologies, Inc. Production of peptides in plants as viral coat protein fusions
GB2262099A (en) * 1991-12-05 1993-06-09 British Tech Group Bicistronic RNA viruses expressing heterologous polypeptide
GB9125896D0 (en) * 1991-12-05 1992-02-05 Almond Jeffrey W Bicistronic viruses
EP0672754B1 (en) 1992-03-31 2004-05-19 Kanebo Ltd. Plant virus vector, plasmid, method of expressing foreign gene, and method of acquiring foreign gene product
WO1995014775A1 (en) 1993-11-26 1995-06-01 British Technology Group Limited Translational enhancer dna
IL112872A0 (en) * 1995-03-03 1995-06-29 Q B I Enterprises Ltd Novel recombinant dna vectors and kits employing them
DE19514310A1 (de) 1995-04-18 1996-10-24 Univ Ludwigs Albert Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen
CA2234931C (en) * 1995-10-16 2010-01-19 Dana-Farber Cancer Institute Novel expression vectors and methods of use

Also Published As

Publication number Publication date
ATE369433T1 (de) 2007-08-15
AU7533998A (en) 1998-12-30
JP2002514086A (ja) 2002-05-14
DE69838222D1 (de) 2007-09-20
FI972293A7 (fi) 1998-12-01
EP1017834A1 (en) 2000-07-12
FI972293L (fi) 1998-12-01
WO1998054342A1 (en) 1998-12-03
FI972293A0 (fi) 1997-05-30
EP1017834B1 (en) 2007-08-08
CA2291803A1 (en) 1998-12-03
US6376745B1 (en) 2002-04-23
DE69838222T2 (de) 2008-02-21

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