CA2289776C - Clone infectieux associe au virus parainfluenza de type 3 d'origine humaine - Google Patents
Clone infectieux associe au virus parainfluenza de type 3 d'origine humaine Download PDFInfo
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- CA2289776C CA2289776C CA002289776A CA2289776A CA2289776C CA 2289776 C CA2289776 C CA 2289776C CA 002289776 A CA002289776 A CA 002289776A CA 2289776 A CA2289776 A CA 2289776A CA 2289776 C CA2289776 C CA 2289776C
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- C—CHEMISTRY; METALLURGY
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
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- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
- C12N2760/18621—Viruses as such, e.g. new isolates, mutants or their genomic sequences
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- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
- C12N2760/18622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
- C12N2760/18634—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
- C12N2760/18651—Methods of production or purification of viral material
- C12N2760/18652—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Cette invention se rapporte à un système de génération d'un virus parainfluenza recombiné d'origine humaine, et notamment du virus parainfluenza de type 3 (HPIV-3) d'origine humaine, recombiné, infectieux. Selon une réalisation, ce système comporte un clone comprenant une séquence nucléotidique qui code un anti-genome, de sens positif, pleine longueur, du HPIV et au moins un clone support comprenant une séquence nucléotidique qui code la protéine P du HPIV et la protéine L du HPIV. Selon une autre réalisation, le système comporte également un clone support qui comprend une séquence nucléotidique qui code la protéine NP du HPIV. La présente invention se rapporte également à un clone qui comporte une séquence nucléotidique codant l'anti-génome, de sens positif, pleine longueur, de HPIV-3. Ce clone comporte également un promoteur d'ARN polymérase lié à la séquence de codage de l'anti-génome du HPIV-3. Dans une réalisation préférée, le clone comporte également une séquence nucléotidique qui code un ribozyme situé immédiatement en aval de la séquence codant l'anti-génome du HPIV-3. La présente invention se rapporte à un procédé de préparation du virus recombiné de HPIV-3 présentant des mutations spécifiques d'un site dans le génome du HPIV-3. Ce procédé consiste à préparer un clone comportant une séquence modifiée codant l'anti-génome de HPIV-3; à introduire dans des cellules hôtes le clone modifié de HPIV-3 et des clones de support qui comportent des séquences nucléotidiques codant une protéine P de HPIV, une protéine L de HPIV et de préférence une protéine NP de HPIV; et à cultiver les cellules hôtes dans des conditions favorables à la synthèse de l'anti-génome modifié de HPIV et des protéines L, P et NP de HPIV-3.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4580597P | 1997-05-07 | 1997-05-07 | |
| US60/045,805 | 1997-05-07 | ||
| PCT/US1998/009270 WO1998050405A1 (fr) | 1997-05-07 | 1998-05-06 | Clone infectieux associe au virus parainfluenza de type 3 d'origine humaine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2289776A1 CA2289776A1 (fr) | 1998-11-12 |
| CA2289776C true CA2289776C (fr) | 2008-10-21 |
Family
ID=21939986
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002289776A Expired - Fee Related CA2289776C (fr) | 1997-05-07 | 1998-05-06 | Clone infectieux associe au virus parainfluenza de type 3 d'origine humaine |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US6248578B1 (fr) |
| EP (1) | EP0981537B1 (fr) |
| JP (1) | JP2002512519A (fr) |
| KR (2) | KR100585946B1 (fr) |
| CN (1) | CN1208340C (fr) |
| AT (1) | ATE367397T1 (fr) |
| AU (1) | AU736586B2 (fr) |
| BR (1) | BR9809240A (fr) |
| CA (1) | CA2289776C (fr) |
| CY (1) | CY1107730T1 (fr) |
| DE (1) | DE69838097T2 (fr) |
| DK (1) | DK0981537T3 (fr) |
| ES (1) | ES2289782T3 (fr) |
| HK (1) | HK1025973A1 (fr) |
| PT (1) | PT981537E (fr) |
| WO (1) | WO1998050405A1 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7465574B2 (en) | 1994-09-30 | 2008-12-16 | Medimmune, Llc | Recombinant RSV virus expression systems and vaccines |
| US6830748B1 (en) | 1997-09-26 | 2004-12-14 | Medimmune Vaccines, Inc. | Recombinant RSV virus expression systems and vaccines |
| US6764685B1 (en) * | 2000-03-21 | 2004-07-20 | Medimmune Vaccines, Inc. | Recombinant parainfluenza virus expression systems and vaccines |
| CN101098958A (zh) * | 2002-02-21 | 2008-01-02 | 免疫医疗疫苗公司 | 间质肺病毒株及其在疫苗制剂中以及用作抗原性序列表达载体的用途 |
| US20100316991A1 (en) * | 2009-06-11 | 2010-12-16 | Utah State University | Human parainfluenza virus type 3 expressing the enhanced green fluorescent protein for use in high-throughput antiviral assays |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5166057A (en) | 1989-08-28 | 1992-11-24 | The Mount Sinai School Of Medicine Of The City University Of New York | Recombinant negative strand rna virus expression-systems |
| EP0702085B2 (fr) | 1994-07-18 | 2010-01-13 | Conzelmann, Karl-Klaus, Prof. Dr. | Virus ARN récombinant, infectieux, non-segmenté, brin-négatif |
| US5869036A (en) * | 1995-12-08 | 1999-02-09 | St. Louis University | Live attenuated vaccines based on CP45 HPIV-3 strain and method to ensure attenuation in such vaccine |
-
1998
- 1998-05-06 AT AT98922118T patent/ATE367397T1/de not_active IP Right Cessation
- 1998-05-06 EP EP98922118A patent/EP0981537B1/fr not_active Expired - Lifetime
- 1998-05-06 CN CNB988048833A patent/CN1208340C/zh not_active Expired - Fee Related
- 1998-05-06 DE DE69838097T patent/DE69838097T2/de not_active Expired - Fee Related
- 1998-05-06 KR KR1019997010246A patent/KR100585946B1/ko not_active IP Right Cessation
- 1998-05-06 KR KR1020067002175A patent/KR100678818B1/ko not_active IP Right Cessation
- 1998-05-06 PT PT98922118T patent/PT981537E/pt unknown
- 1998-05-06 US US09/073,492 patent/US6248578B1/en not_active Expired - Fee Related
- 1998-05-06 DK DK98922118T patent/DK0981537T3/da active
- 1998-05-06 ES ES98922118T patent/ES2289782T3/es not_active Expired - Lifetime
- 1998-05-06 CA CA002289776A patent/CA2289776C/fr not_active Expired - Fee Related
- 1998-05-06 BR BR9809240-5A patent/BR9809240A/pt not_active Application Discontinuation
- 1998-05-06 WO PCT/US1998/009270 patent/WO1998050405A1/fr active IP Right Grant
- 1998-05-06 JP JP54846598A patent/JP2002512519A/ja active Pending
- 1998-05-06 AU AU74731/98A patent/AU736586B2/en not_active Ceased
-
2000
- 2000-08-21 HK HK00105223A patent/HK1025973A1/xx not_active IP Right Cessation
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2007
- 2007-09-10 CY CY20071101163T patent/CY1107730T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0981537B1 (fr) | 2007-07-18 |
| AU736586B2 (en) | 2001-08-02 |
| US6248578B1 (en) | 2001-06-19 |
| BR9809240A (pt) | 2000-06-27 |
| CY1107730T1 (el) | 2013-04-18 |
| HK1025973A1 (en) | 2000-12-01 |
| KR100585946B1 (ko) | 2006-06-07 |
| JP2002512519A (ja) | 2002-04-23 |
| EP0981537A4 (fr) | 2002-09-18 |
| WO1998050405A1 (fr) | 1998-11-12 |
| CN1208340C (zh) | 2005-06-29 |
| ATE367397T1 (de) | 2007-08-15 |
| DE69838097T2 (de) | 2008-04-10 |
| KR100678818B1 (ko) | 2007-02-07 |
| KR20060014461A (ko) | 2006-02-15 |
| DK0981537T3 (da) | 2007-11-19 |
| CA2289776A1 (fr) | 1998-11-12 |
| KR20010012294A (ko) | 2001-02-15 |
| AU7473198A (en) | 1998-11-27 |
| DE69838097D1 (de) | 2007-08-30 |
| PT981537E (pt) | 2007-10-16 |
| ES2289782T3 (es) | 2008-02-01 |
| CN1255140A (zh) | 2000-05-31 |
| EP0981537A1 (fr) | 2000-03-01 |
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