CA2260940C - Methods for isolating nucleic acids using alkaline protease - Google Patents

Methods for isolating nucleic acids using alkaline protease Download PDF

Info

Publication number
CA2260940C
CA2260940C CA002260940A CA2260940A CA2260940C CA 2260940 C CA2260940 C CA 2260940C CA 002260940 A CA002260940 A CA 002260940A CA 2260940 A CA2260940 A CA 2260940A CA 2260940 C CA2260940 C CA 2260940C
Authority
CA
Canada
Prior art keywords
solution
nucleic acid
alkaline
dna
protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002260940A
Other languages
English (en)
French (fr)
Other versions
CA2260940A1 (en
Inventor
John Shultz
Craig E. Smith
Douglas R. Storts
Paula Brisco
Judy Frederiksen Towne
Susanne Selman
Josephine Grosch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of CA2260940A1 publication Critical patent/CA2260940A1/en
Application granted granted Critical
Publication of CA2260940C publication Critical patent/CA2260940C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
CA002260940A 1996-07-29 1997-07-29 Methods for isolating nucleic acids using alkaline protease Expired - Fee Related CA2260940C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/681,922 1996-07-29
US08/681,922 US5981235A (en) 1996-07-29 1996-07-29 Methods for isolating nucleic acids using alkaline protease
PCT/US1997/013440 WO1998004730A1 (en) 1996-07-29 1997-07-29 Methods for isolating nucleic acids using alkaline protease

Publications (2)

Publication Number Publication Date
CA2260940A1 CA2260940A1 (en) 1998-02-05
CA2260940C true CA2260940C (en) 2007-05-15

Family

ID=24737412

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002260940A Expired - Fee Related CA2260940C (en) 1996-07-29 1997-07-29 Methods for isolating nucleic acids using alkaline protease

Country Status (7)

Country Link
US (1) US5981235A (enExample)
EP (1) EP0918877B1 (enExample)
JP (1) JP3943601B2 (enExample)
AU (1) AU729932B2 (enExample)
CA (1) CA2260940C (enExample)
DE (1) DE69730974T2 (enExample)
WO (1) WO1998004730A1 (enExample)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3847779B2 (ja) * 1994-02-07 2006-11-22 クイアジェン・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング 遺伝子治療のためのエンドトキシンを含まないかエンドトキシンを減少させた核酸及び／またはオリゴヌクレオチドの製造方法
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
DE69805649T2 (de) * 1997-12-06 2002-12-05 Dna Res Innovations Ltd Isolierung von nukleinsäuren
US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US6194562B1 (en) * 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification
US6180408B1 (en) * 1998-08-21 2001-01-30 Washington University Fluorescence polarization in nucleic acid analysis
AU2862100A (en) * 1999-01-27 2000-08-18 Folim G. Halaka Materials and methods for the purification of polyelectrolytes
WO2000063362A1 (en) * 1999-04-21 2000-10-26 Annovis, Inc. Magnetic dna extraction kit for plants
US20020012990A1 (en) 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
US20030228600A1 (en) * 2000-07-14 2003-12-11 Eppendorf 5 Prime, Inc. DNA isolation method and kit
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
DE10121463A1 (de) * 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
KR100454869B1 (ko) * 2001-07-13 2004-11-03 주식회사 인트론바이오테크놀로지 Ｄｎａ 추출용 세포 용해 버퍼 및 이를 이용한 ｄｎａ 추출방법
DE10153957A1 (de) * 2001-11-06 2003-05-22 Quiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren
ATE449186T1 (de) 2001-11-28 2009-12-15 Applied Biosystems Llc Zusammensetzungen und verfahren zur selektiven nukleinsäureisolierung
AU2003202026A1 (en) 2002-01-16 2003-09-02 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
US7364846B2 (en) * 2002-10-11 2008-04-29 Molecular Devices Corporation Gene expression profiling from FFPE samples
JP2006507818A (ja) * 2002-11-01 2006-03-09 プロメガ　コーポレイション 細胞溶解のための組成物、使用方法、装置およびキット
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
US7601491B2 (en) * 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
EP1633763A1 (en) * 2003-06-04 2006-03-15 Qiagen AS Process for the concentration and/or isolation of nucleic acid or nucleic acid-containing species
US20050164204A1 (en) * 2004-01-27 2005-07-28 Reed Thomas D. Single use lyophilized rnase reagents, and kits and methods for using same
AU2005200670B2 (en) * 2004-02-20 2007-05-03 F. Hoffmann-La Roche Ag Adsorption of nucleic acids to a solid phase
KR100486179B1 (ko) * 2004-03-02 2005-05-03 피경태 핵산을 분리하고 정제하기 위한 세포 용해 조성물, 방법 및 키트
US7794932B2 (en) 2004-04-16 2010-09-14 Piotr Chomczynski Reagents and methods for isolation of purified RNA
JP4971997B2 (ja) 2005-01-31 2012-07-11 メルク・シャープ・エンド・ドーム・コーポレイション プラスミドｄｎａの精製方法
US20060188892A1 (en) * 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
CA2613094A1 (en) * 2005-07-01 2007-01-11 Promega Corporation Network of buoyant particles for biomolecule purification
EP1749892B1 (en) 2005-08-02 2008-03-19 Roche Diagnostics GmbH Nucleotide sequence for assessing TSE
US7569367B2 (en) 2005-09-07 2009-08-04 Roche Diagnostics Operations, Inc. Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy
US8030034B2 (en) * 2005-12-09 2011-10-04 Promega Corporation Nucleic acid purification with a binding matrix
EP1932913B1 (en) 2006-12-11 2013-01-16 Roche Diagnostics GmbH Nucleic acid isolation using polidocanol and derivatives
JP5354894B2 (ja) 2006-12-11 2013-11-27 エフ．ホフマン−ラ ロシュ アーゲー ポリドカノールおよび誘導体を用いた核酸単離
US8535888B2 (en) 2006-12-29 2013-09-17 Mayo Foundation For Medical Education And Research Compositions and methods for detecting methicillin-resistant S. aureus
DK2181191T3 (da) * 2007-08-20 2011-05-23 Novozymes As Nukleasereduktion
US8007205B2 (en) * 2008-04-09 2011-08-30 Marshall Iii Henry Bennie Foundation wall vapor barrier system
ES2439951T3 (es) 2008-06-06 2014-01-27 F. Hoffmann-La Roche Ag Detección y cuantificación multiplex de ácidos nucleicos microbianos controlada de forma interna
CN101619355B (zh) * 2009-07-02 2012-02-08 黑龙江大学 传统发酵豆酱中细菌的检测方法
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
WO2011026194A1 (en) * 2009-09-07 2011-03-10 Reproductive Health Science Pty Ltd Nucleic acid extraction
WO2012013732A1 (en) 2010-07-29 2012-02-02 F. Hoffmann - La Roche Ag Generic pcr
DE112011102524T5 (de) 2010-07-29 2013-07-11 Roche Diagnostics Gmbh Präparation generischer Proben
WO2012013734A1 (en) 2010-07-29 2012-02-02 F. Hoffmann - La Roche Ag Control nucleic acids for multiple parameters
CA2802548C (en) 2010-07-29 2017-01-03 F. Hoffmann-La Roche Ag Qualitative and quantitative detection of microbial nucleic acids
EP2426222A1 (en) 2010-09-07 2012-03-07 F. Hoffmann-La Roche AG Generic buffer for amplification
EP2465945A1 (en) 2010-12-17 2012-06-20 F. Hoffmann-La Roche AG Generic matrix for control nucleic acids
WO2013067069A2 (en) * 2011-10-31 2013-05-10 Leinweber Barbara Dual digestion method for high yield nucleic acid recovery
US9057673B2 (en) 2012-08-24 2015-06-16 The Board Of Trustees Of The Leland Stanford Junior University Method of preparing RNA from ribonuclease-rich sources
US20160010081A1 (en) * 2014-07-11 2016-01-14 Exact Sciences Corporation Systems, methods and kits for extracting nucleic acid from digestive fluid
US10000750B2 (en) 2014-10-09 2018-06-19 Promega Corporation Method of isolating nucleic acid
US10364428B2 (en) 2014-11-14 2019-07-30 Corning Incorporated Methods and kits for post-IVT RNA purification
WO2016094465A1 (en) * 2014-12-09 2016-06-16 Corning Incorporated Methods and kits for purifying plasmid dna
US11015217B2 (en) 2015-05-29 2021-05-25 Roche Molecular Systems, Inc. Method of inactivating microbes by citraconic anhydride
EP3916090B1 (en) 2016-01-29 2025-07-23 Purigen Biosystems, Inc. Isotachophoresis for purification of nucleic acids
WO2019028197A1 (en) 2017-08-02 2019-02-07 Purigen Biosystems, Inc. SYSTEMS, DEVICES AND METHODS FOR ISOTACHOPHORESIS
GB201803654D0 (en) * 2018-03-07 2018-04-25 Arcticzymes As Thermolabile proteinases
KR20230139598A (ko) 2022-03-28 2023-10-05 씨제이제일제당 (주) O-아세틸 호모세린 생산 미생물 및 이를 이용한 o-아세틸 호모세린 또는 l-메치오닌 생산 방법
KR20230139597A (ko) 2022-03-28 2023-10-05 씨제이제일제당 (주) O-아세틸 호모세린 생산 미생물 및 이를 이용한 o-아세틸 호모세린 또는 l-메치오닌 생산 방법
KR20240152456A (ko) 2023-04-12 2024-10-22 씨제이제일제당 (주) 피루브산 탈수소효소 복합체 서브유닛 e1의 활성이 증가된 미생물 및 이를 이용한 o-아세틸호모세린 또는 그의 유도체의 생산 방법
GB202319423D0 (en) * 2023-12-18 2024-01-31 BioNTech SE Measuring and reducing protein impurities

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
US4843155A (en) * 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US5075430A (en) * 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
US5256557A (en) * 1991-12-27 1993-10-26 Solvay Enzymes, Inc. Purified alkaline protease concentrate and method of preparation
US5386024A (en) * 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
EP0708828B1 (en) * 1993-07-08 1997-03-12 Tepnel Medical Limited Obtaining nucleic acids
WO1995006652A1 (en) * 1993-08-30 1995-03-09 Promega Corporation Nucleic acid purification compositions and methods
US5646263A (en) * 1994-09-19 1997-07-08 Promega Corporation High efficiency method for isolating target substances using a multisample separation device

Also Published As

Publication number Publication date
CA2260940A1 (en) 1998-02-05
AU3821497A (en) 1998-02-20
EP0918877A4 (en) 2001-01-31
DE69730974D1 (de) 2004-11-04
AU729932B2 (en) 2001-02-15
EP0918877B1 (en) 2004-09-29
EP0918877A1 (en) 1999-06-02
DE69730974T2 (de) 2006-02-23
US5981235A (en) 1999-11-09
JP2000516091A (ja) 2000-12-05
WO1998004730A1 (en) 1998-02-05
JP3943601B2 (ja) 2007-07-11

Similar Documents

Publication Publication Date Title
CA2260940C (en) Methods for isolating nucleic acids using alkaline protease
WO1998004730A9 (en) Methods for isolating nucleic acids using alkaline protease
US7767804B2 (en) Compositions and methods for using a solid support to purify RNA
US6218531B1 (en) Method of isolating RNA
JP3477205B2 (ja) 酸プロテアーゼを用いた核酸の調製
CN101124321B (zh) 用于从稳定剂中纯化核酸的组合物和方法
EP1641944B1 (en) Room temperature elution of nucleic acids
JP5354894B2 (ja) ポリドカノールおよび誘導体を用いた核酸単離
KR20010080625A (ko) 임의의 복잡한 출발물질로부터 핵산을 분리하기 위한배합물과 방법 그리고 복합한 유전자 분석법
US20040157223A1 (en) Chemical treatment of biological samples for nucleic acid extraction and kits therefor
JP2005501523A (ja) 界面活性剤およびプロテアーゼを使用して核酸を単離するための組成物、方法およびキット
JP2008531028A (ja) カオトロピック剤および／または界面活性剤の存在下で核酸を分解するためのヌクレアーゼの使用
JP2024012495A (ja) 核酸を抽出するための方法
US6281349B1 (en) Method for nucleic acid purification using iodine
CN109295054A (zh) 用于靶向病原体基因RNA的gRNA及基于C2c2的病原体基因的检测方法、检测试剂盒
Thatcher Nucleic acid isolation
AU2004233035B2 (en) Compositions and methods for using a solid support to purify RNA
JP2002522093A (ja) 核酸を含む生物学的標本中に潜在的に存在する機能の分離及び特徴づけ方法
HK1178567B (en) Nucleic acid isolation using polidocanol and derivatives
HK1118859B (en) Nucleic acid isolation using polidocanol and derivatives

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed