AU729932B2 - Methods for isolating nucleic acids using alkaline protease - Google Patents
Methods for isolating nucleic acids using alkaline protease Download PDFInfo
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- AU729932B2 AU729932B2 AU38214/97A AU3821497A AU729932B2 AU 729932 B2 AU729932 B2 AU 729932B2 AU 38214/97 A AU38214/97 A AU 38214/97A AU 3821497 A AU3821497 A AU 3821497A AU 729932 B2 AU729932 B2 AU 729932B2
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- solution
- nucleic acid
- alkaline
- dna
- protease
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Classifications
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- C—CHEMISTRY; METALLURGY
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/681922 | 1996-07-29 | ||
| US08/681,922 US5981235A (en) | 1996-07-29 | 1996-07-29 | Methods for isolating nucleic acids using alkaline protease |
| PCT/US1997/013440 WO1998004730A1 (en) | 1996-07-29 | 1997-07-29 | Methods for isolating nucleic acids using alkaline protease |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU3821497A AU3821497A (en) | 1998-02-20 |
| AU729932B2 true AU729932B2 (en) | 2001-02-15 |
Family
ID=24737412
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU38214/97A Ceased AU729932B2 (en) | 1996-07-29 | 1997-07-29 | Methods for isolating nucleic acids using alkaline protease |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5981235A (enExample) |
| EP (1) | EP0918877B1 (enExample) |
| JP (1) | JP3943601B2 (enExample) |
| AU (1) | AU729932B2 (enExample) |
| CA (1) | CA2260940C (enExample) |
| DE (1) | DE69730974T2 (enExample) |
| WO (1) | WO1998004730A1 (enExample) |
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| JP3847779B2 (ja) * | 1994-02-07 | 2006-11-22 | クイアジェン・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング | 遺伝子治療のためのエンドトキシンを含まないかエンドトキシンを減少させた核酸及び／またはオリゴヌクレオチドの製造方法 |
| GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| DE69805649T2 (de) * | 1997-12-06 | 2002-12-05 | Dna Res Innovations Ltd | Isolierung von nukleinsäuren |
| US7078224B1 (en) * | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
| US6194562B1 (en) * | 1998-04-22 | 2001-02-27 | Promega Corporation | Endotoxin reduction in nucleic acid purification |
| US6180408B1 (en) * | 1998-08-21 | 2001-01-30 | Washington University | Fluorescence polarization in nucleic acid analysis |
| AU2862100A (en) * | 1999-01-27 | 2000-08-18 | Folim G. Halaka | Materials and methods for the purification of polyelectrolytes |
| WO2000063362A1 (en) * | 1999-04-21 | 2000-10-26 | Annovis, Inc. | Magnetic dna extraction kit for plants |
| US20020012990A1 (en) | 1999-12-22 | 2002-01-31 | Lander Russel Jackson | Process for the scaleable purification of plasmid DNA |
| US20030228600A1 (en) * | 2000-07-14 | 2003-12-11 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
| US7001724B1 (en) | 2000-11-28 | 2006-02-21 | Applera Corporation | Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases |
| DE10121463A1 (de) * | 2001-05-02 | 2003-02-27 | Henkel Kgaa | Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten |
| KR100454869B1 (ko) * | 2001-07-13 | 2004-11-03 | 주식회사 인트론바이오테크놀로지 | Ｄｎａ 추출용 세포 용해 버퍼 및 이를 이용한 ｄｎａ 추출방법 |
| DE10153957A1 (de) * | 2001-11-06 | 2003-05-22 | Quiagen Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| ATE449186T1 (de) | 2001-11-28 | 2009-12-15 | Applied Biosystems Llc | Zusammensetzungen und verfahren zur selektiven nukleinsäureisolierung |
| AU2003202026A1 (en) | 2002-01-16 | 2003-09-02 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
| US7364846B2 (en) * | 2002-10-11 | 2008-04-29 | Molecular Devices Corporation | Gene expression profiling from FFPE samples |
| JP2006507818A (ja) * | 2002-11-01 | 2006-03-09 | プロメガ コーポレイション | 細胞溶解のための組成物、使用方法、装置およびキット |
| US20040157219A1 (en) * | 2003-02-06 | 2004-08-12 | Jianrong Lou | Chemical treatment of biological samples for nucleic acid extraction and kits therefor |
| US7601491B2 (en) * | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
| EP1633763A1 (en) * | 2003-06-04 | 2006-03-15 | Qiagen AS | Process for the concentration and/or isolation of nucleic acid or nucleic acid-containing species |
| US20050164204A1 (en) * | 2004-01-27 | 2005-07-28 | Reed Thomas D. | Single use lyophilized rnase reagents, and kits and methods for using same |
| AU2005200670B2 (en) * | 2004-02-20 | 2007-05-03 | F. Hoffmann-La Roche Ag | Adsorption of nucleic acids to a solid phase |
| KR100486179B1 (ko) * | 2004-03-02 | 2005-05-03 | 피경태 | 핵산을 분리하고 정제하기 위한 세포 용해 조성물, 방법 및 키트 |
| US7794932B2 (en) | 2004-04-16 | 2010-09-14 | Piotr Chomczynski | Reagents and methods for isolation of purified RNA |
| JP4971997B2 (ja) | 2005-01-31 | 2012-07-11 | メルク・シャープ・エンド・ドーム・コーポレイション | プラスミドｄｎａの精製方法 |
| US20060188892A1 (en) * | 2005-02-18 | 2006-08-24 | Ambion, Inc. | Enzymatic digestion of tissue |
| CA2613094A1 (en) * | 2005-07-01 | 2007-01-11 | Promega Corporation | Network of buoyant particles for biomolecule purification |
| EP1749892B1 (en) | 2005-08-02 | 2008-03-19 | Roche Diagnostics GmbH | Nucleotide sequence for assessing TSE |
| US7569367B2 (en) | 2005-09-07 | 2009-08-04 | Roche Diagnostics Operations, Inc. | Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy |
| US8030034B2 (en) * | 2005-12-09 | 2011-10-04 | Promega Corporation | Nucleic acid purification with a binding matrix |
| EP1932913B1 (en) | 2006-12-11 | 2013-01-16 | Roche Diagnostics GmbH | Nucleic acid isolation using polidocanol and derivatives |
| JP5354894B2 (ja) | 2006-12-11 | 2013-11-27 | エフ．ホフマン−ラ ロシュ アーゲー | ポリドカノールおよび誘導体を用いた核酸単離 |
| US8535888B2 (en) | 2006-12-29 | 2013-09-17 | Mayo Foundation For Medical Education And Research | Compositions and methods for detecting methicillin-resistant S. aureus |
| DK2181191T3 (da) * | 2007-08-20 | 2011-05-23 | Novozymes As | Nukleasereduktion |
| US8007205B2 (en) * | 2008-04-09 | 2011-08-30 | Marshall Iii Henry Bennie | Foundation wall vapor barrier system |
| ES2439951T3 (es) | 2008-06-06 | 2014-01-27 | F. Hoffmann-La Roche Ag | Detección y cuantificación multiplex de ácidos nucleicos microbianos controlada de forma interna |
| CN101619355B (zh) * | 2009-07-02 | 2012-02-08 | 黑龙江大学 | 传统发酵豆酱中细菌的检测方法 |
| US8039613B2 (en) | 2009-08-28 | 2011-10-18 | Promega Corporation | Methods of purifying a nucleic acid and formulation and kit for use in performing such methods |
| US8222397B2 (en) | 2009-08-28 | 2012-07-17 | Promega Corporation | Methods of optimal purification of nucleic acids and kit for use in performing such methods |
| WO2011026194A1 (en) * | 2009-09-07 | 2011-03-10 | Reproductive Health Science Pty Ltd | Nucleic acid extraction |
| WO2012013732A1 (en) | 2010-07-29 | 2012-02-02 | F. Hoffmann - La Roche Ag | Generic pcr |
| DE112011102524T5 (de) | 2010-07-29 | 2013-07-11 | Roche Diagnostics Gmbh | Präparation generischer Proben |
| WO2012013734A1 (en) | 2010-07-29 | 2012-02-02 | F. Hoffmann - La Roche Ag | Control nucleic acids for multiple parameters |
| CA2802548C (en) | 2010-07-29 | 2017-01-03 | F. Hoffmann-La Roche Ag | Qualitative and quantitative detection of microbial nucleic acids |
| EP2426222A1 (en) | 2010-09-07 | 2012-03-07 | F. Hoffmann-La Roche AG | Generic buffer for amplification |
| EP2465945A1 (en) | 2010-12-17 | 2012-06-20 | F. Hoffmann-La Roche AG | Generic matrix for control nucleic acids |
| WO2013067069A2 (en) * | 2011-10-31 | 2013-05-10 | Leinweber Barbara | Dual digestion method for high yield nucleic acid recovery |
| US9057673B2 (en) | 2012-08-24 | 2015-06-16 | The Board Of Trustees Of The Leland Stanford Junior University | Method of preparing RNA from ribonuclease-rich sources |
| US20160010081A1 (en) * | 2014-07-11 | 2016-01-14 | Exact Sciences Corporation | Systems, methods and kits for extracting nucleic acid from digestive fluid |
| US10000750B2 (en) | 2014-10-09 | 2018-06-19 | Promega Corporation | Method of isolating nucleic acid |
| US10364428B2 (en) | 2014-11-14 | 2019-07-30 | Corning Incorporated | Methods and kits for post-IVT RNA purification |
| WO2016094465A1 (en) * | 2014-12-09 | 2016-06-16 | Corning Incorporated | Methods and kits for purifying plasmid dna |
| US11015217B2 (en) | 2015-05-29 | 2021-05-25 | Roche Molecular Systems, Inc. | Method of inactivating microbes by citraconic anhydride |
| EP3916090B1 (en) | 2016-01-29 | 2025-07-23 | Purigen Biosystems, Inc. | Isotachophoresis for purification of nucleic acids |
| WO2019028197A1 (en) | 2017-08-02 | 2019-02-07 | Purigen Biosystems, Inc. | SYSTEMS, DEVICES AND METHODS FOR ISOTACHOPHORESIS |
| GB201803654D0 (en) * | 2018-03-07 | 2018-04-25 | Arcticzymes As | Thermolabile proteinases |
| KR20230139598A (ko) | 2022-03-28 | 2023-10-05 | 씨제이제일제당 (주) | O-아세틸 호모세린 생산 미생물 및 이를 이용한 o-아세틸 호모세린 또는 l-메치오닌 생산 방법 |
| KR20230139597A (ko) | 2022-03-28 | 2023-10-05 | 씨제이제일제당 (주) | O-아세틸 호모세린 생산 미생물 및 이를 이용한 o-아세틸 호모세린 또는 l-메치오닌 생산 방법 |
| KR20240152456A (ko) | 2023-04-12 | 2024-10-22 | 씨제이제일제당 (주) | 피루브산 탈수소효소 복합체 서브유닛 e1의 활성이 증가된 미생물 및 이를 이용한 o-아세틸호모세린 또는 그의 유도체의 생산 방법 |
| GB202319423D0 (en) * | 2023-12-18 | 2024-01-31 | BioNTech SE | Measuring and reducing protein impurities |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4900677A (en) * | 1986-09-26 | 1990-02-13 | E. I. Du Pont De Nemours And Company | Process for rapid isolation of high molecular weight DNA |
| US5386024A (en) * | 1993-02-10 | 1995-01-31 | Gen-Probe Incorporated | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
| WO1995006652A1 (en) * | 1993-08-30 | 1995-03-09 | Promega Corporation | Nucleic acid purification compositions and methods |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4843155A (en) * | 1987-11-19 | 1989-06-27 | Piotr Chomczynski | Product and process for isolating RNA |
| US5075430A (en) * | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
| US5155018A (en) * | 1991-07-10 | 1992-10-13 | Hahnemann University | Process and kit for isolating and purifying RNA from biological sources |
| US5256557A (en) * | 1991-12-27 | 1993-10-26 | Solvay Enzymes, Inc. | Purified alkaline protease concentrate and method of preparation |
| EP0708828B1 (en) * | 1993-07-08 | 1997-03-12 | Tepnel Medical Limited | Obtaining nucleic acids |
| US5646263A (en) * | 1994-09-19 | 1997-07-08 | Promega Corporation | High efficiency method for isolating target substances using a multisample separation device |
-
1996
- 1996-07-29 US US08/681,922 patent/US5981235A/en not_active Expired - Lifetime
-
1997
- 1997-07-29 CA CA002260940A patent/CA2260940C/en not_active Expired - Fee Related
- 1997-07-29 JP JP50913998A patent/JP3943601B2/ja not_active Expired - Fee Related
- 1997-07-29 AU AU38214/97A patent/AU729932B2/en not_active Ceased
- 1997-07-29 WO PCT/US1997/013440 patent/WO1998004730A1/en not_active Ceased
- 1997-07-29 DE DE69730974T patent/DE69730974T2/de not_active Expired - Lifetime
- 1997-07-29 EP EP97935224A patent/EP0918877B1/en not_active Expired - Lifetime
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4900677A (en) * | 1986-09-26 | 1990-02-13 | E. I. Du Pont De Nemours And Company | Process for rapid isolation of high molecular weight DNA |
| US5386024A (en) * | 1993-02-10 | 1995-01-31 | Gen-Probe Incorporated | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
| WO1995006652A1 (en) * | 1993-08-30 | 1995-03-09 | Promega Corporation | Nucleic acid purification compositions and methods |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2260940A1 (en) | 1998-02-05 |
| CA2260940C (en) | 2007-05-15 |
| AU3821497A (en) | 1998-02-20 |
| EP0918877A4 (en) | 2001-01-31 |
| DE69730974D1 (de) | 2004-11-04 |
| EP0918877B1 (en) | 2004-09-29 |
| EP0918877A1 (en) | 1999-06-02 |
| DE69730974T2 (de) | 2006-02-23 |
| US5981235A (en) | 1999-11-09 |
| JP2000516091A (ja) | 2000-12-05 |
| WO1998004730A1 (en) | 1998-02-05 |
| JP3943601B2 (ja) | 2007-07-11 |
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