CA2226518A1 - (s)-hydroxynitrillyase from hevea brasiliensis - Google Patents
(s)-hydroxynitrillyase from hevea brasiliensis Download PDFInfo
- Publication number
- CA2226518A1 CA2226518A1 CA002226518A CA2226518A CA2226518A1 CA 2226518 A1 CA2226518 A1 CA 2226518A1 CA 002226518 A CA002226518 A CA 002226518A CA 2226518 A CA2226518 A CA 2226518A CA 2226518 A1 CA2226518 A1 CA 2226518A1
- Authority
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- Canada
- Prior art keywords
- protein
- hnl
- nitrile
- hydroxy
- lyase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- 238000012216 screening Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- RPACBEVZENYWOL-XFULWGLBSA-M sodium;(2r)-2-[6-(4-chlorophenoxy)hexyl]oxirane-2-carboxylate Chemical compound [Na+].C=1C=C(Cl)C=CC=1OCCCCCC[C@]1(C(=O)[O-])CO1 RPACBEVZENYWOL-XFULWGLBSA-M 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 108010061238 threonyl-glycine Proteins 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/002—Nitriles (-CN)
- C12P13/004—Cyanohydrins
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Detergent Compositions (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT0118295A AT404837B (de) | 1995-07-12 | 1995-07-12 | (s)-hydroxynitrillyase aus hevea brasiliensis |
| ATA1182/95 | 1995-07-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2226518A1 true CA2226518A1 (en) | 1997-01-30 |
Family
ID=3508388
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002226518A Abandoned CA2226518A1 (en) | 1995-07-12 | 1996-07-10 | (s)-hydroxynitrillyase from hevea brasiliensis |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US6046042A (enExample) |
| EP (1) | EP0840792B1 (enExample) |
| JP (1) | JP4090076B2 (enExample) |
| AT (2) | AT404837B (enExample) |
| CA (1) | CA2226518A1 (enExample) |
| DE (1) | DE59608804D1 (enExample) |
| DK (1) | DK0840792T3 (enExample) |
| ES (1) | ES2170251T3 (enExample) |
| IL (1) | IL122606A (enExample) |
| TW (1) | TW469293B (enExample) |
| WO (1) | WO1997003204A2 (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0799894B1 (de) * | 1996-02-09 | 2004-05-19 | Degussa AG | Verfahren zur Herstellung von (S)-Cyanhydrinen |
| AT407397B (de) | 1998-07-02 | 2001-02-26 | Dsm Fine Chem Austria Gmbh | (s)-hydroxynitrillyasen mit verbesserter substratakzeptanz und deren verwendung |
| DE69928206T2 (de) | 1998-12-28 | 2006-08-03 | Nippon Shokubai Co., Ltd. | Verfahren zur Herstellung von S-Hydroxynitrillyasen |
| US6194039B1 (en) * | 1999-10-22 | 2001-02-27 | Elsicon, Inc. | Materials for inducing alignment in liquid crystals and liquid crystal displays |
| DE19963485B4 (de) * | 1999-12-28 | 2006-02-16 | JFC - Jülich Fine Chemicals GmbH | Verfahren zur Herstellung von Hydroxylnitril-Lyasen |
| EP1566441B1 (de) * | 2001-01-16 | 2009-02-18 | DSM Fine Chemicals Austria Nfg GmbH & Co KG | Gene, enthaltend eine für Hydroxynitrillyase codierende DNA-Sequenz, rekombinante Proteine mit Hydroxynitrillyase-Aktivität und deren Verwendung |
| AT412157B (de) * | 2002-07-15 | 2004-10-25 | Dsm Fine Chem Austria Gmbh | Screeningmethode zum nachweis von amidase- und nitrilhydrataseaktivitäten und deren verwendung |
| JP4124639B2 (ja) * | 2002-12-17 | 2008-07-23 | 株式会社日本触媒 | 大腸菌を用いたｓ−ヒドロキシニトリルリアーゼの製造方法 |
| AT412092B (de) * | 2003-02-27 | 2004-09-27 | Dsm Fine Chem Austria Gmbh | Verfahren zur herstellung chiraler alpha-hydroxycarbonsäuren durch enzymatische hydrolyse von chiralen cyanhydrinen |
| WO2005095602A1 (en) * | 2004-03-31 | 2005-10-13 | Nippon Shokubai Co., Ltd. | Novel modified s-hydroxynitrile lyase |
| JP4854202B2 (ja) * | 2004-03-31 | 2012-01-18 | 株式会社日本触媒 | 新規改変型ｓ−ヒドロキシニトリルリアーゼ |
| EP1811028B1 (en) | 2004-10-15 | 2013-04-10 | Mitsubishi Rayon Co., Ltd. | Improved hydroxynitrile lyase |
| WO2008071695A1 (en) | 2006-12-14 | 2008-06-19 | Dsm Ip Assets Bv | R-hnl random variants and their use for preparing optically pure, sterically hindered cyanohydrins |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT396252B (de) * | 1991-10-31 | 1993-07-26 | Chemie Linz Gmbh | Enzymatisches verfahren zur enantioselektiven herstellung optisch aktiver cyanhydrine |
-
1995
- 1995-07-12 AT AT0118295A patent/AT404837B/de not_active IP Right Cessation
-
1996
- 1996-07-10 IL IL12260696A patent/IL122606A/xx not_active IP Right Cessation
- 1996-07-10 DK DK96925698T patent/DK0840792T3/da active
- 1996-07-10 AT AT96925698T patent/ATE213776T1/de not_active IP Right Cessation
- 1996-07-10 JP JP50550297A patent/JP4090076B2/ja not_active Expired - Lifetime
- 1996-07-10 EP EP96925698A patent/EP0840792B1/de not_active Expired - Lifetime
- 1996-07-10 ES ES96925698T patent/ES2170251T3/es not_active Expired - Lifetime
- 1996-07-10 DE DE59608804T patent/DE59608804D1/de not_active Expired - Lifetime
- 1996-07-10 US US08/981,256 patent/US6046042A/en not_active Expired - Lifetime
- 1996-07-10 WO PCT/EP1996/003010 patent/WO1997003204A2/de not_active Ceased
- 1996-07-10 CA CA002226518A patent/CA2226518A1/en not_active Abandoned
- 1996-07-11 TW TW085108400A patent/TW469293B/zh not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| TW469293B (en) | 2001-12-21 |
| ATA118295A (de) | 1998-07-15 |
| EP0840792B1 (de) | 2002-02-27 |
| ES2170251T3 (es) | 2002-08-01 |
| IL122606A0 (en) | 1998-08-16 |
| US6046042A (en) | 2000-04-04 |
| DE59608804D1 (en) | 2002-04-04 |
| EP0840792A2 (de) | 1998-05-13 |
| WO1997003204A2 (de) | 1997-01-30 |
| WO1997003204A3 (de) | 1997-03-20 |
| IL122606A (en) | 2005-11-20 |
| JPH11508775A (ja) | 1999-08-03 |
| DK0840792T3 (da) | 2002-04-22 |
| JP4090076B2 (ja) | 2008-05-28 |
| AT404837B (de) | 1999-03-25 |
| ATE213776T1 (de) | 2002-03-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |