CA2172840C - Methode de mesure utilisant un echantillon de sang entier - Google Patents

Methode de mesure utilisant un echantillon de sang entier Download PDF

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Publication number
CA2172840C
CA2172840C CA002172840A CA2172840A CA2172840C CA 2172840 C CA2172840 C CA 2172840C CA 002172840 A CA002172840 A CA 002172840A CA 2172840 A CA2172840 A CA 2172840A CA 2172840 C CA2172840 C CA 2172840C
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CA
Canada
Prior art keywords
partner
whole blood
complex
analyte
blood sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002172840A
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English (en)
Other versions
CA2172840A1 (fr
Inventor
Nobuhiro Hoshino
Michiko Kawamoto
Mitoshi Shimamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LSI Medience Corp
Original Assignee
Mitsubishi Kagaku Iatron Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kagaku Iatron Inc filed Critical Mitsubishi Kagaku Iatron Inc
Priority claimed from PCT/JP1995/001510 external-priority patent/WO1996004558A1/fr
Publication of CA2172840A1 publication Critical patent/CA2172840A1/fr
Application granted granted Critical
Publication of CA2172840C publication Critical patent/CA2172840C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/02—Hepadnaviridae, e.g. hepatitis B virus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Une quantité appropriée d'un échantillon de sang entier est maintenue en contact avec un support insoluble recouvert d'une première substance associée pouvant se lier spécifiquement à un objet de mesure pour un court laps de temps. Après que la combinaison de l'objet avec la première substance associée sur le support insoluble est mise en contact avec une seconde substance associée par liaison spécifique à l'objet, le complexe qui en résulte comprenant le support insoluble, la première substance associée, l'objet à mesurer et la seconde substance associée marquée est séparé d'une partie ne contenant pas le complexe. Ensuite, des signaux émanant de la substance marquée contenue dans l'une ou l'autre des parties séparées sont détectés. Selon cette méthode, le sang entier peut être utilisé en tant que tel sans prétraitement de l'échantillon et l'objet à mesurer dans l'échantillon de sang entier peut être mesuré rapidement.
CA002172840A 1994-07-29 1995-07-28 Methode de mesure utilisant un echantillon de sang entier Expired - Lifetime CA2172840C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP6/197207 1994-07-29
JP19720794 1994-07-29
PCT/JP1995/001510 WO1996004558A1 (fr) 1994-07-29 1995-07-28 Systeme de mesure fonctionnant avec du sang entier

Publications (2)

Publication Number Publication Date
CA2172840A1 CA2172840A1 (fr) 1996-02-15
CA2172840C true CA2172840C (fr) 2007-10-23

Family

ID=38650021

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002172840A Expired - Lifetime CA2172840C (fr) 1994-07-29 1995-07-28 Methode de mesure utilisant un echantillon de sang entier

Country Status (1)

Country Link
CA (1) CA2172840C (fr)

Also Published As

Publication number Publication date
CA2172840A1 (fr) 1996-02-15

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