CA2166918A1 - Dispositif de digestion d'organe et procede pour isoler des cellules ou amas de cellules d'interet biologique a partir d'un organe - Google Patents
Dispositif de digestion d'organe et procede pour isoler des cellules ou amas de cellules d'interet biologique a partir d'un organeInfo
- Publication number
- CA2166918A1 CA2166918A1 CA002166918A CA2166918A CA2166918A1 CA 2166918 A1 CA2166918 A1 CA 2166918A1 CA 002166918 A CA002166918 A CA 002166918A CA 2166918 A CA2166918 A CA 2166918A CA 2166918 A1 CA2166918 A1 CA 2166918A1
- Authority
- CA
- Canada
- Prior art keywords
- organ
- enclosure
- container
- cells
- digestion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 210000000056 organ Anatomy 0.000 title claims abstract description 101
- 230000029087 digestion Effects 0.000 title claims description 69
- 238000000034 method Methods 0.000 title claims description 24
- 230000008569 process Effects 0.000 title description 4
- 239000007788 liquid Substances 0.000 claims abstract description 52
- 239000012634 fragment Substances 0.000 claims abstract description 11
- 238000004891 communication Methods 0.000 claims abstract description 8
- 238000005119 centrifugation Methods 0.000 claims description 9
- 210000002808 connective tissue Anatomy 0.000 claims description 9
- 206010001497 Agitation Diseases 0.000 claims description 7
- 238000013019 agitation Methods 0.000 claims description 7
- 230000005484 gravity Effects 0.000 claims description 5
- 230000009471 action Effects 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000008188 pellet Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 230000015556 catabolic process Effects 0.000 claims description 2
- 238000010907 mechanical stirring Methods 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 239000007791 liquid phase Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 50
- 210000000496 pancreas Anatomy 0.000 description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 239000012530 fluid Substances 0.000 description 9
- 238000002955 isolation Methods 0.000 description 8
- 238000000746 purification Methods 0.000 description 7
- 102000029816 Collagenase Human genes 0.000 description 6
- 108060005980 Collagenase Proteins 0.000 description 6
- 239000012981 Hank's balanced salt solution Substances 0.000 description 6
- 229960002424 collagenase Drugs 0.000 description 6
- 210000004153 islets of langerhan Anatomy 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 238000002054 transplantation Methods 0.000 description 5
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 230000003393 splenic effect Effects 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108700042768 University of Wisconsin-lactobionate solution Proteins 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000008105 immune reaction Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- VNHGETRQQSYUGZ-UHFFFAOYSA-N 1-nitro-2-phenoxybenzene Chemical compound [O-][N+](=O)C1=CC=CC=C1OC1=CC=CC=C1 VNHGETRQQSYUGZ-UHFFFAOYSA-N 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 201000004569 Blindness Diseases 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 208000001647 Renal Insufficiency Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000000560 biocompatible material Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 210000001608 connective tissue cell Anatomy 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002900 effect on cell Effects 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- NTHXOOBQLCIOLC-UHFFFAOYSA-N iohexol Chemical compound OCC(O)CN(C(=O)C)C1=C(I)C(C(=O)NCC(O)CO)=C(I)C(C(=O)NCC(O)CO)=C1I NTHXOOBQLCIOLC-UHFFFAOYSA-N 0.000 description 1
- 201000006370 kidney failure Diseases 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 210000004798 organs belonging to the digestive system Anatomy 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/09—Means for pre-treatment of biological substances by enzymatic treatment
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9500677A FR2729398A1 (fr) | 1995-01-17 | 1995-01-17 | Dispositif de digestion d'organe et procede pour isoler des cellules ou amas de cellules d'interet biologique a partir d'un organe |
| FR9500677 | 1995-01-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2166918A1 true CA2166918A1 (fr) | 1996-07-18 |
Family
ID=9475366
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002166918A Abandoned CA2166918A1 (fr) | 1995-01-17 | 1996-01-10 | Dispositif de digestion d'organe et procede pour isoler des cellules ou amas de cellules d'interet biologique a partir d'un organe |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0723009A1 (enExample) |
| JP (1) | JPH08280376A (enExample) |
| CA (1) | CA2166918A1 (enExample) |
| FR (1) | FR2729398A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5786207A (en) * | 1997-05-28 | 1998-07-28 | University Of Pittsburgh | Tissue dissociating system and method |
| AU2003235875A1 (en) * | 2002-05-07 | 2003-11-11 | Asahi Kasei Kabushiki Kaisha | Method and system for obtaining cells of specific type from biological material |
| US11174458B2 (en) | 2007-04-23 | 2021-11-16 | Koligo Therapeutics, Inc. | Cell separation apparatus and methods of use |
| US20100112696A1 (en) * | 2008-11-03 | 2010-05-06 | Baxter International Inc. | Apparatus And Methods For Processing Tissue To Release Cells |
| US8309343B2 (en) | 2008-12-01 | 2012-11-13 | Baxter International Inc. | Apparatus and method for processing biological material |
| JP5837742B2 (ja) | 2010-08-27 | 2015-12-24 | 学校法人東京女子医科大学 | 細胞単離装置 |
| CA3123386A1 (en) * | 2018-12-13 | 2020-06-18 | Tissue Genesis, Llc | Cell separation apparatus and methods of use |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1356794A (en) * | 1970-11-20 | 1974-06-12 | Defence Secratary Of State For | Apparatus and method for the production of a single cell suspen sion from tissue |
| US4264741A (en) * | 1979-06-05 | 1981-04-28 | New Brunswick Scientific Co., Inc. | Tiltable fermentor |
| GB8604497D0 (en) * | 1986-02-24 | 1986-04-03 | Connaught Lab | Separation of islets of langerhans |
| US5254472A (en) * | 1992-02-24 | 1993-10-19 | Brooks Iii Edward H | Organic digesting system |
| FR2696754B1 (fr) * | 1992-10-14 | 1994-11-18 | Hospal Ind | Méthode d'isolement de cellules à partir d'un organe et dispositif pour sa mise en Óoeuvre. |
| US5409833A (en) * | 1993-07-01 | 1995-04-25 | Baxter International Inc. | Microvessel cell isolation apparatus |
-
1995
- 1995-01-17 FR FR9500677A patent/FR2729398A1/fr active Granted
-
1996
- 1996-01-09 EP EP96420009A patent/EP0723009A1/fr not_active Withdrawn
- 1996-01-10 CA CA002166918A patent/CA2166918A1/fr not_active Abandoned
- 1996-01-17 JP JP8035335A patent/JPH08280376A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP0723009A1 (fr) | 1996-07-24 |
| JPH08280376A (ja) | 1996-10-29 |
| FR2729398A1 (fr) | 1996-07-19 |
| FR2729398B1 (enExample) | 1997-02-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |