CA2016211A1 - Subtilisines ayant subi plusieurs mutations - Google Patents
Subtilisines ayant subi plusieurs mutationsInfo
- Publication number
- CA2016211A1 CA2016211A1 CA2016211A CA2016211A CA2016211A1 CA 2016211 A1 CA2016211 A1 CA 2016211A1 CA 2016211 A CA2016211 A CA 2016211A CA 2016211 A CA2016211 A CA 2016211A CA 2016211 A1 CA2016211 A1 CA 2016211A1
- Authority
- CA
- Canada
- Prior art keywords
- subtilisins
- multiply mutated
- subtilisin
- mutated subtilisins
- multiply
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Cosmetics (AREA)
- Tires In General (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35312489A | 1989-05-17 | 1989-05-17 | |
US353,124 | 1989-05-17 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2016211A1 true CA2016211A1 (fr) | 1990-11-17 |
CA2016211C CA2016211C (fr) | 1998-07-21 |
Family
ID=23387862
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002016211A Expired - Lifetime CA2016211C (fr) | 1989-05-17 | 1990-05-07 | Subtilisines ayant subi plusieurs mutations |
Country Status (14)
Country | Link |
---|---|
US (1) | US5397705A (fr) |
EP (1) | EP0398539B1 (fr) |
JP (1) | JPH03505976A (fr) |
KR (1) | KR100188532B1 (fr) |
AT (1) | ATE141324T1 (fr) |
AU (1) | AU618675B2 (fr) |
CA (1) | CA2016211C (fr) |
DE (1) | DE69028040T2 (fr) |
DK (1) | DK0398539T3 (fr) |
ES (1) | ES2091224T3 (fr) |
GR (1) | GR3021010T3 (fr) |
HK (1) | HK1006857A1 (fr) |
SG (1) | SG47797A1 (fr) |
WO (1) | WO1990014420A1 (fr) |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3155984B2 (ja) * | 1987-04-06 | 2001-04-16 | ノヴォザイムズ・アクティーゼルスカブ | タンパク質の安定化のための金属イオン結合部位における静電的相互作用の操作 |
US5665587A (en) * | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
DK271490D0 (da) * | 1990-11-14 | 1990-11-14 | Novo Nordisk As | Detergentkomposition |
US5863783A (en) * | 1991-03-27 | 1999-01-26 | Gist-Brocades, N.V. | Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin |
HUT66846A (en) * | 1991-10-16 | 1995-01-30 | Unilever Nv | Aqueous enzymatic detergent compositions |
US5989887A (en) * | 1992-11-25 | 1999-11-23 | Dsm N.V. | Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin |
US5567601A (en) * | 1993-06-01 | 1996-10-22 | University Of Maryland | Subtilisin mutants lacking a primary calcium binding site |
US6436690B1 (en) | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
US6440717B1 (en) | 1993-09-15 | 2002-08-27 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis |
MA23346A1 (fr) * | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
CA2173106C (fr) * | 1993-10-14 | 2000-02-15 | Michael Eugene Burns | Compositions contenant un agent de blanchiment et des proteases |
ATE361355T1 (de) * | 1993-10-14 | 2007-05-15 | Procter & Gamble | Proteasehaltige reinigungsmittel |
DE4411223A1 (de) * | 1994-03-31 | 1995-10-05 | Solvay Enzymes Gmbh & Co Kg | Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren |
US6599730B1 (en) | 1994-05-02 | 2003-07-29 | Procter & Gamble Company | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
US6066611A (en) * | 1994-10-13 | 2000-05-23 | The Procter & Gamble Company | Bleaching compositions comprising protease enzymes |
US6455295B1 (en) | 1995-03-08 | 2002-09-24 | The Procter & Gamble Company | Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis |
IL117350A0 (en) | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
US6475765B1 (en) | 1995-03-09 | 2002-11-05 | Procter & Gamble Company | Subtilisin DY variants having decreased adsorption and increased hydrolysis |
CN1117842C (zh) * | 1996-12-20 | 2003-08-13 | 尤尼利弗公司 | 酶促漂白组合物 |
US6204234B1 (en) * | 1997-07-09 | 2001-03-20 | The Proctor & Gamble Company | Cleaning compositions comprising a specific oxygenase |
ES2368718T3 (es) * | 1997-10-23 | 2011-11-21 | Danisco Us Inc. | Variantes de subtilisina con múltiples sustituciones. |
MA24811A1 (fr) * | 1997-10-23 | 1999-12-31 | Procter & Gamble | Compositions de lavage contenant des variantes de proteases multisubstituees |
BR9909130A (pt) * | 1998-03-26 | 2000-12-19 | Procter & Gamble | Variantes de protease de serina tendo substituições de amino-ácido |
US6908757B1 (en) | 1998-03-26 | 2005-06-21 | The Procter & Gamble Company | Serine protease variants having amino acid deletions and substitutions |
CZ2002171A3 (cs) | 1999-07-22 | 2002-06-12 | The Procter & Gamble Company | Proteinázový konjugát, čistící prostředek a prostředek osobní péče |
BR0012660A (pt) | 1999-07-22 | 2002-04-09 | Procter & Gamble | Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal |
CN1373802A (zh) | 1999-07-22 | 2002-10-09 | 宝洁公司 | 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体 |
US6946128B1 (en) | 1999-07-22 | 2005-09-20 | The Procter & Gamble Company | Protease conjugates having sterically protected epitope regions |
US7217554B2 (en) | 1999-08-31 | 2007-05-15 | Novozymes A/S | Proteases and variants thereof |
EP1214426A2 (fr) * | 1999-08-31 | 2002-06-19 | Novozymes A/S | Nouvelles proteases et leurs variants |
US6727085B2 (en) * | 1999-12-15 | 2004-04-27 | Fanoe Tina Sejersgaard | Subtilase variants having an improved wash performance on egg stains |
AU2001280968A1 (en) * | 2000-07-31 | 2002-02-13 | Menzel, Rolf | Compositions and methods for directed gene assembly |
ES2309167T3 (es) | 2001-02-19 | 2008-12-16 | Merck Patent Gmbh | Metodo para identificar epitotes de celulas t y metodo para preparar moleculas con inmunogenicidad reducida. |
US6929939B2 (en) * | 2001-03-23 | 2005-08-16 | Genencor International, Inc. | Proteins producing an altered immunogenic response and methods of making and using the same |
US20050227229A1 (en) * | 2001-07-09 | 2005-10-13 | Lebo Roger V | Multiple controls for molecular genetic analyses |
DE10153792A1 (de) | 2001-10-31 | 2003-05-22 | Henkel Kgaa | Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten |
EP2287320B1 (fr) * | 2002-01-16 | 2014-10-01 | Danisco US Inc. | Variantes multiples de protéase substituée |
US7888093B2 (en) * | 2002-11-06 | 2011-02-15 | Novozymes A/S | Subtilase variants |
US7294499B2 (en) | 2003-01-30 | 2007-11-13 | Novozymes A/S | Subtilases |
US7727756B2 (en) * | 2003-03-21 | 2010-06-01 | Novozymes A/S | Subtilases |
CA2526341C (fr) * | 2003-05-07 | 2013-02-19 | Novozymes A/S | Enzymes de subtilisine variant (subtilases) |
WO2020112599A1 (fr) * | 2018-11-28 | 2020-06-04 | Danisco Us Inc | Variants de subtilisine à stabilité améliorée |
WO2023225459A2 (fr) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions et procédés de prévention, de traitement, de suppression et/ou d'élimination d'infestations et d'infections phytopathogènes |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4760025A (en) * | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
US5155033A (en) * | 1989-01-06 | 1992-10-13 | Genencor, Inc. | Subtilisins modified at position 225 resulting in a shift in catalytic activity |
EP0254735B2 (fr) * | 1986-01-15 | 1998-06-17 | Amgen Inc. | PROCEDE DE PRODUCTION D'ANALOGUES DE SUBTILISINE THERMIQUEMENT STABLE ET STABLE AU pH |
US4980288A (en) * | 1986-02-12 | 1990-12-25 | Genex Corporation | Subtilisin with increased thermal stability |
US4990452A (en) * | 1986-02-12 | 1991-02-05 | Genex Corporation | Combining mutations for stabilization of subtilisin |
US5013657A (en) * | 1988-04-12 | 1991-05-07 | Bryan Philip N | Subtilisin mutations |
DE3750916T2 (de) * | 1986-04-30 | 1995-06-01 | Genencor Int | Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte. |
NZ221627A (en) * | 1986-09-09 | 1993-04-28 | Genencor Inc | Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios |
ES2134186T3 (es) * | 1987-02-27 | 1999-10-01 | Genencor Int | Clonaje molecular y expresion de genes que codifican enzimas proteoliticas. |
JP3155984B2 (ja) * | 1987-04-06 | 2001-04-16 | ノヴォザイムズ・アクティーゼルスカブ | タンパク質の安定化のための金属イオン結合部位における静電的相互作用の操作 |
US4914031A (en) * | 1987-04-10 | 1990-04-03 | Amgen, Inc. | Subtilisin analogs |
FR2614467A1 (fr) * | 1987-04-21 | 1988-10-28 | Telemecanique Electrique | Appareil interrupteur de protection a mecanisme de declenchement simplifie |
-
1990
- 1990-04-23 AU AU55580/90A patent/AU618675B2/en not_active Expired
- 1990-04-23 WO PCT/US1990/002234 patent/WO1990014420A1/fr unknown
- 1990-04-23 JP JP2507107A patent/JPH03505976A/ja active Pending
- 1990-04-23 KR KR1019910700067A patent/KR100188532B1/ko not_active IP Right Cessation
- 1990-05-01 EP EP90304715A patent/EP0398539B1/fr not_active Expired - Lifetime
- 1990-05-01 DK DK90304715.7T patent/DK0398539T3/da active
- 1990-05-01 ES ES90304715T patent/ES2091224T3/es not_active Expired - Lifetime
- 1990-05-01 DE DE69028040T patent/DE69028040T2/de not_active Expired - Lifetime
- 1990-05-01 SG SG1996004414A patent/SG47797A1/en unknown
- 1990-05-01 AT AT90304715T patent/ATE141324T1/de not_active IP Right Cessation
- 1990-05-07 CA CA002016211A patent/CA2016211C/fr not_active Expired - Lifetime
-
1992
- 1992-03-25 US US07/857,714 patent/US5397705A/en not_active Expired - Lifetime
-
1996
- 1996-09-12 GR GR960402360T patent/GR3021010T3/el unknown
-
1998
- 1998-06-23 HK HK98106058A patent/HK1006857A1/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DE69028040D1 (de) | 1996-09-19 |
SG47797A1 (en) | 1998-04-17 |
AU618675B2 (en) | 1992-01-02 |
GR3021010T3 (en) | 1996-12-31 |
EP0398539A1 (fr) | 1990-11-22 |
KR920701426A (ko) | 1992-08-11 |
ATE141324T1 (de) | 1996-08-15 |
JPH03505976A (ja) | 1991-12-26 |
DK0398539T3 (da) | 1996-12-09 |
AU5558090A (en) | 1990-12-18 |
DE69028040T2 (de) | 1997-02-27 |
CA2016211C (fr) | 1998-07-21 |
ES2091224T3 (es) | 1996-11-01 |
HK1006857A1 (en) | 1999-03-19 |
US5397705A (en) | 1995-03-14 |
WO1990014420A1 (fr) | 1990-11-29 |
EP0398539B1 (fr) | 1996-08-14 |
KR100188532B1 (ko) | 1999-06-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |