CA1303532C - Determination of fibrin using fibrin-specific antibodies - Google Patents
Determination of fibrin using fibrin-specific antibodiesInfo
- Publication number
- CA1303532C CA1303532C CA000471712A CA471712A CA1303532C CA 1303532 C CA1303532 C CA 1303532C CA 000471712 A CA000471712 A CA 000471712A CA 471712 A CA471712 A CA 471712A CA 1303532 C CA1303532 C CA 1303532C
- Authority
- CA
- Canada
- Prior art keywords
- fibrin
- antibodies
- fibrinogen
- peptide
- val
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 229950003499 fibrin Drugs 0.000 title claims abstract description 43
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 108010073385 Fibrin Proteins 0.000 title claims abstract description 34
- 102000009123 Fibrin Human genes 0.000 title claims abstract description 34
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 25
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 5
- 230000002163 immunogen Effects 0.000 claims abstract 3
- 108010049003 Fibrinogen Proteins 0.000 claims description 20
- 102000008946 Fibrinogen Human genes 0.000 claims description 20
- 229940012952 fibrinogen Drugs 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 19
- 150000001413 amino acids Chemical group 0.000 claims description 13
- 235000001014 amino acid Nutrition 0.000 claims description 10
- 125000006850 spacer group Chemical group 0.000 claims description 5
- JWICNZAGYSIBAR-LEEGLKINSA-N (4s)-4-[[2-[[(2s)-2-[[(2s)-2-[[(2s)-2-aminopropanoyl]amino]-3-carboxypropanoyl]amino]-3-hydroxypropanoyl]amino]acetyl]amino]-5-[[2-[[(2s)-3-carboxy-1-[[(2s)-1-[[1-[[(2s)-1-[[(2s)-4-carboxy-1-[[2-[[2-[[2-[[(2s)-1-[[(1s)-1-carboxy-4-(diaminomethylideneamino Chemical compound NC(N)=NCCC[C@@H](C(O)=O)NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)CNC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)C(CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](C)N)CC1=CC=CC=C1 JWICNZAGYSIBAR-LEEGLKINSA-N 0.000 claims description 3
- 101800000974 Fibrinopeptide A Proteins 0.000 claims description 3
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- DSVOSLJAGTUKFB-SPAGYVKCSA-N (2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-1-(2-aminoacetyl)pyrrolidine-2-carbonyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]-3-methylbutanoyl]amino]-3-methylbutanoyl]amino]pentanedioic acid Chemical compound OC(=O)CC[C@@H](C(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H]1CCCN1C(=O)CN DSVOSLJAGTUKFB-SPAGYVKCSA-N 0.000 claims description 2
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- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 8
- 229940024606 amino acid Drugs 0.000 description 8
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- 108060003951 Immunoglobulin Proteins 0.000 description 2
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 2
- 102000015636 Oligopeptides Human genes 0.000 description 2
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- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 2
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- 125000003277 amino group Chemical group 0.000 description 2
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- XHXFXVLFKHQFAL-UHFFFAOYSA-N phosphoryl trichloride Chemical compound ClP(Cl)(Cl)=O XHXFXVLFKHQFAL-UHFFFAOYSA-N 0.000 description 2
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- 125000002221 trityl group Chemical group [H]C1=C([H])C([H])=C([H])C([H])=C1C([*])(C1=C(C(=C(C(=C1[H])[H])[H])[H])[H])C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 2
- 229940008841 1,6-hexamethylene diisocyanate Drugs 0.000 description 1
- ASOKPJOREAFHNY-UHFFFAOYSA-N 1-Hydroxybenzotriazole Chemical compound C1=CC=C2N(O)N=NC2=C1 ASOKPJOREAFHNY-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- NJXWZWXCHBNOOG-UHFFFAOYSA-N 3,3-diphenylpropyl(1-phenylethyl)azanium;chloride Chemical compound [Cl-].C=1C=CC=CC=1C(C)[NH2+]CCC(C=1C=CC=CC=1)C1=CC=CC=C1 NJXWZWXCHBNOOG-UHFFFAOYSA-N 0.000 description 1
- TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010027612 Batroxobin Proteins 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
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- 239000007995 HEPES buffer Substances 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- 229930182821 L-proline Natural products 0.000 description 1
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- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
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- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
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- 241000700159 Rattus Species 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
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- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
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- 239000007983 Tris buffer Substances 0.000 description 1
- UPJONISHZRADBH-XPUUQOCRSA-N Val-Glu Chemical compound CC(C)[C@H](N)C(=O)N[C@H](C(O)=O)CCC(O)=O UPJONISHZRADBH-XPUUQOCRSA-N 0.000 description 1
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- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
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- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical compound CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 1
- UXGNZZKBCMGWAZ-UHFFFAOYSA-N dimethylformamide dmf Chemical compound CN(C)C=O.CN(C)C=O UXGNZZKBCMGWAZ-UHFFFAOYSA-N 0.000 description 1
- 208000009190 disseminated intravascular coagulation Diseases 0.000 description 1
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- 125000002795 guanidino group Chemical group C(N)(=N)N* 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- RRAMGCGOFNQTLD-UHFFFAOYSA-N hexamethylene diisocyanate Chemical compound O=C=NCCCCCCN=C=O RRAMGCGOFNQTLD-UHFFFAOYSA-N 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
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- WVDDGKGOMKODPV-ZQBYOMGUSA-N phenyl(114C)methanol Chemical compound O[14CH2]C1=CC=CC=C1 WVDDGKGOMKODPV-ZQBYOMGUSA-N 0.000 description 1
- UYWQUFXKFGHYNT-UHFFFAOYSA-N phenylmethyl ester of formic acid Natural products O=COCC1=CC=CC=C1 UYWQUFXKFGHYNT-UHFFFAOYSA-N 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
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- 229960002429 proline Drugs 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
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- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
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- 229940104230 thymidine Drugs 0.000 description 1
- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
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- 229960004295 valine Drugs 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/745—Assays involving non-enzymic blood coagulation factors
- G01N2333/75—Fibrin; Fibrinogen
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Electrotherapy Devices (AREA)
- Eye Examination Apparatus (AREA)
- Magnetic Resonance Imaging Apparatus (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP3400434.3 | 1984-01-09 | ||
| DE19843400434 DE3400434A1 (de) | 1984-01-09 | 1984-01-09 | Bestimmung von fibrin mit fibrin-spezifischen antikoerpern |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1303532C true CA1303532C (en) | 1992-06-16 |
Family
ID=6224505
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000471712A Expired - Lifetime CA1303532C (en) | 1984-01-09 | 1985-01-08 | Determination of fibrin using fibrin-specific antibodies |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0152612B1 (enrdf_load_stackoverflow) |
| JP (1) | JPS60158353A (enrdf_load_stackoverflow) |
| AT (1) | ATE50458T1 (enrdf_load_stackoverflow) |
| AU (1) | AU595479B2 (enrdf_load_stackoverflow) |
| CA (1) | CA1303532C (enrdf_load_stackoverflow) |
| DE (2) | DE3400434A1 (enrdf_load_stackoverflow) |
| ES (1) | ES8606411A1 (enrdf_load_stackoverflow) |
| NO (1) | NO166591B (enrdf_load_stackoverflow) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4916070A (en) * | 1986-04-14 | 1990-04-10 | The General Hospital Corporation | Fibrin-specific antibodies and method of screening for the antibodies |
| DE3632343A1 (de) * | 1986-09-24 | 1988-04-07 | Battelle Institut E V | Verfahren zum nachweis von 1,2,2-trimethylpropylmethylphosphofluoridad |
| DE3701812A1 (de) * | 1987-01-22 | 1988-08-04 | Max Planck Gesellschaft | Fibrinspezifischer antikoerper, verfahren zur seiner herstellung und seine verwendung |
| SE8701437L (sv) * | 1987-04-06 | 1988-10-07 | Rolf Kenth Sigvard Wallin | Diagnostiskt foerfarande in vitro samt medel som kan utnyttjas vid foerfarandet |
| DE3730059A1 (de) * | 1987-09-08 | 1989-03-30 | Behringwerke Ag | Verfahren zur bestimmung von "loeslichem" fibrin |
| NL8801227A (nl) * | 1988-05-10 | 1989-12-01 | Tno | Antilichamen tegen fibrine; voor de bereiding van de antilichamen te gebruiken immunogeen, werkwijze voor het bepalen van fibrine met de antilichamen en farmaceutisch preparaat op basis van de antilichamen. |
| US5609869A (en) * | 1988-08-19 | 1997-03-11 | The General Hospital Corporation | Hybrid immunoglobulin-thrombolytic enzyme molecules which specifically bind a thrombus, and methods of their production and use |
| US5811265A (en) * | 1988-08-19 | 1998-09-22 | The General Hospital Corporation | Hybrid immunoglobulin-thrombolytic enzyme molecules which specifically bind a thrombus, and methods of their production and use |
| DE4134833A1 (de) * | 1991-09-25 | 1993-04-01 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung von fibrin |
| ATE138478T1 (de) * | 1992-02-17 | 1996-06-15 | Akzo Nobel Nv | Kalibrator und verwendung davon in einer immuntest |
| DE4420742A1 (de) * | 1993-10-20 | 1995-04-27 | Boehringer Mannheim Gmbh | Synthetischer Standard für Immunoassays |
| US5780255A (en) * | 1995-06-09 | 1998-07-14 | Instrumentation Laboratory, S.P.A. | Protein C pathway screening test |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2532151C3 (de) * | 1975-07-18 | 1979-06-13 | Biotest-Serum-Institut Gmbh, 6000 Frankfurt | Antisenim zur quantitativen Bestimmung der Abbauprodukte des Fibrins und Fibrinogen« und Verfahren zu seiner Herstellung |
| JPS5811856A (ja) * | 1981-07-16 | 1983-01-22 | Mihama Hisaharu | 抗フラグメントＤγ−２量体血清の製造方法 |
| US4438209A (en) * | 1981-07-17 | 1984-03-20 | Mallinckrodt, Inc. | Radioimmunoassay for fibrinopeptide A |
| AU572125B2 (en) * | 1983-03-17 | 1988-05-05 | Mabco Limited | Monoclonal antibodies with specificity for crosslinked fibrin and their diagnotic uses |
| US4722903A (en) * | 1983-11-14 | 1988-02-02 | New York Blood Center, Inc. | Monoclonal antibodies specific to in vivo fragments derived from human fibrinogen, human fiberin I or human fibrin II |
-
1984
- 1984-01-09 DE DE19843400434 patent/DE3400434A1/de active Granted
- 1984-12-20 EP EP84116008A patent/EP0152612B1/de not_active Expired - Lifetime
- 1984-12-20 DE DE8484116008T patent/DE3481386D1/de not_active Expired - Lifetime
- 1984-12-20 AT AT84116008T patent/ATE50458T1/de not_active IP Right Cessation
-
1985
- 1985-01-07 ES ES539382A patent/ES8606411A1/es not_active Expired
- 1985-01-08 AU AU37521/85A patent/AU595479B2/en not_active Ceased
- 1985-01-08 CA CA000471712A patent/CA1303532C/en not_active Expired - Lifetime
- 1985-01-08 NO NO850087A patent/NO166591B/no unknown
- 1985-01-08 JP JP60000471A patent/JPS60158353A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| ES8606411A1 (es) | 1986-04-01 |
| JPS60158353A (ja) | 1985-08-19 |
| NO850087L (no) | 1985-07-10 |
| DE3481386D1 (de) | 1990-03-29 |
| EP0152612A3 (en) | 1986-04-16 |
| NO166591B (no) | 1991-05-06 |
| ES539382A0 (es) | 1986-04-01 |
| EP0152612B1 (de) | 1990-02-21 |
| EP0152612A2 (de) | 1985-08-28 |
| AU595479B2 (en) | 1990-04-05 |
| ATE50458T1 (de) | 1990-03-15 |
| DE3400434C2 (enrdf_load_stackoverflow) | 1992-11-05 |
| AU3752185A (en) | 1985-07-18 |
| DE3400434A1 (de) | 1985-09-26 |
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| MKLA | Lapsed |