CA1282359C - Bilirubin-specific enzyme and its analytical use - Google Patents
Bilirubin-specific enzyme and its analytical useInfo
- Publication number
- CA1282359C CA1282359C CA000524619A CA524619A CA1282359C CA 1282359 C CA1282359 C CA 1282359C CA 000524619 A CA000524619 A CA 000524619A CA 524619 A CA524619 A CA 524619A CA 1282359 C CA1282359 C CA 1282359C
- Authority
- CA
- Canada
- Prior art keywords
- bilirubin
- enzyme
- range
- isolated
- analyte
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 title claims abstract description 226
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 99
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 99
- 239000000203 mixture Substances 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 28
- 241000196324 Embryophyta Species 0.000 claims abstract description 16
- 238000002360 preparation method Methods 0.000 claims abstract description 15
- 241000208838 Asteraceae Species 0.000 claims abstract description 12
- 238000003556 assay Methods 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims description 31
- 230000008859 change Effects 0.000 claims description 24
- 239000012491 analyte Substances 0.000 claims description 18
- 244000019459 Cynara cardunculus Species 0.000 claims description 17
- 235000016520 artichoke thistle Nutrition 0.000 claims description 16
- 235000019106 Cynara scolymus Nutrition 0.000 claims description 15
- 230000002452 interceptive effect Effects 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 10
- 239000008363 phosphate buffer Substances 0.000 claims description 9
- 239000002244 precipitate Substances 0.000 claims description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 7
- 239000000725 suspension Substances 0.000 claims description 7
- 239000008280 blood Substances 0.000 claims description 6
- 210000004369 blood Anatomy 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 239000012876 carrier material Substances 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 239000002250 absorbent Substances 0.000 claims description 3
- 230000002745 absorbent Effects 0.000 claims description 3
- 230000015556 catabolic process Effects 0.000 claims description 3
- 238000006731 degradation reaction Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 2
- 239000012062 aqueous buffer Substances 0.000 claims 4
- 239000007787 solid Substances 0.000 claims 3
- 238000002156 mixing Methods 0.000 claims 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims 1
- 238000009835 boiling Methods 0.000 claims 1
- 238000001816 cooling Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 102000004169 proteins and genes Human genes 0.000 abstract description 4
- 108090000623 proteins and genes Proteins 0.000 abstract description 4
- 230000000593 degrading effect Effects 0.000 abstract description 3
- 230000007306 turnover Effects 0.000 abstract description 2
- 240000008892 Helianthus tuberosus Species 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 75
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 8
- 239000012530 fluid Substances 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 229960002163 hydrogen peroxide Drugs 0.000 description 4
- 210000004185 liver Anatomy 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000007480 spreading Effects 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 230000009102 absorption Effects 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000723343 Cichorium Species 0.000 description 2
- 241000208818 Helianthus Species 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- 240000008415 Lactuca sativa Species 0.000 description 2
- 235000003228 Lactuca sativa Nutrition 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 description 2
- 210000000941 bile Anatomy 0.000 description 2
- 239000013060 biological fluid Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- -1 poly(ethylene terephthalate) Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- SVTBMSDMJJWYQN-UHFFFAOYSA-N 2-methylpentane-2,4-diol Chemical group CC(O)CC(C)(C)O SVTBMSDMJJWYQN-UHFFFAOYSA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 235000007542 Cichorium intybus Nutrition 0.000 description 1
- 101100536354 Drosophila melanogaster tant gene Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010056740 Genital discharge Diseases 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001082241 Lythrum hyssopifolia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101100101585 Mus musculus Ubqln4 gene Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 241000245665 Taraxacum Species 0.000 description 1
- 240000001949 Taraxacum officinale Species 0.000 description 1
- 235000006754 Taraxacum officinale Nutrition 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 150000001642 boronic acid derivatives Chemical class 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000008199 coating composition Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical class CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000005670 electromagnetic radiation Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000003248 enzyme activator Substances 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 238000005558 fluorometry Methods 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- IZXGZAJMDLJLMF-UHFFFAOYSA-N methylaminomethanol Chemical compound CNCO IZXGZAJMDLJLMF-UHFFFAOYSA-N 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000139 polyethylene terephthalate Polymers 0.000 description 1
- 239000005020 polyethylene terephthalate Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 238000001055 reflectance spectroscopy Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- IOVGROKTTNBUGK-SJCJKPOMSA-N ritodrine Chemical compound N([C@@H](C)[C@H](O)C=1C=CC(O)=CC=1)CCC1=CC=C(O)C=C1 IOVGROKTTNBUGK-SJCJKPOMSA-N 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000003375 selectivity assay Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000002594 sorbent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/728—Bilirubin; including biliverdin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/816—Enzyme separation or purification by solubility
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US866,845 | 1986-05-27 | ||
| US06/866,845 US4746606A (en) | 1986-05-27 | 1986-05-27 | Bilirubin-specific enzyme and its analytical use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1282359C true CA1282359C (en) | 1991-04-02 |
Family
ID=25348553
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000524619A Expired - Fee Related CA1282359C (en) | 1986-05-27 | 1986-12-05 | Bilirubin-specific enzyme and its analytical use |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4746606A (enExample) |
| EP (1) | EP0247846B1 (enExample) |
| JP (1) | JPS62285782A (enExample) |
| CA (1) | CA1282359C (enExample) |
| DE (1) | DE3779179D1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0320095A3 (en) * | 1987-12-10 | 1989-08-02 | HEALTH & RESEARCH SERVICES INC. | Bilirubin degrading enzymes |
| JP2856757B2 (ja) * | 1989-03-13 | 1999-02-10 | ユニチカ株式会社 | 総ビリルビンの測定方法および測定用試薬 |
| DE4406379A1 (de) * | 1993-03-24 | 1994-09-29 | Boehringer Mannheim Gmbh | Bilirubinoxidase aus Alfalfa und Verwendung des Enzyms |
| DE19627376A1 (de) * | 1996-07-06 | 1998-01-08 | Aar Pharma Adler Apotheke | Verwendung von Artischocken-(Cynara)-Extrakten |
| US20030003464A1 (en) * | 2000-11-27 | 2003-01-02 | Phan Brigitte C. | Dual bead assays including optical biodiscs and methods relating thereto |
| US7504243B2 (en) | 2004-03-19 | 2009-03-17 | The United States Of America As Represented By The Department Of Health And Human Services | Methods for the production of biliverdin |
| FR2957934B1 (fr) | 2010-03-24 | 2014-10-17 | Centre Nat Rech Scient | Bilirubine oxydase de bacillus pumilus et ses applications |
| CN103517703B (zh) | 2011-05-11 | 2016-11-09 | 天野酶制品株式会社 | 染色剂及其用途 |
| FR2975704B1 (fr) | 2011-05-24 | 2015-02-20 | Centre Nat Rech Scient | Bilirubine oxydase de magnaporthe oryzae et ses applications |
| EP4582549A1 (en) | 2022-09-02 | 2025-07-09 | Amano Enzyme Inc. | Phenylacetaldehyde production method and use application thereof |
| EP4721580A1 (en) | 2023-05-26 | 2026-04-08 | Amano Enzyme Inc. | Method for producing aldehyde compound and application thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4211844A (en) * | 1978-05-19 | 1980-07-08 | Eastman Kodak Company | Bilirubin-specific fungal enzyme preparation |
| JPS6012031B2 (ja) * | 1981-03-30 | 1985-03-29 | 天野製薬株式会社 | ビリルビンオキシダ−ゼの製造法 |
| JPS59135886A (ja) * | 1983-01-25 | 1984-08-04 | Takara Shuzo Co Ltd | ビリルビン・オキシダ−ゼの製造法 |
| JPS59198971A (ja) * | 1983-04-28 | 1984-11-10 | Kyowa Hakko Kogyo Co Ltd | ビリルビンオキシダ−ゼの製造法 |
| US4701411A (en) * | 1983-09-01 | 1987-10-20 | Eastman Kodak Company | Bilirubin-specific enzyme preparation, assay compositions, analytical elements and methods using same |
| US4600689A (en) * | 1983-11-21 | 1986-07-15 | Takara Shuzo Co., Ltd. | Novel bilirubin oxidase, its production and use |
-
1986
- 1986-05-27 US US06/866,845 patent/US4746606A/en not_active Expired - Fee Related
- 1986-12-05 CA CA000524619A patent/CA1282359C/en not_active Expired - Fee Related
-
1987
- 1987-05-26 JP JP62127295A patent/JPS62285782A/ja active Granted
- 1987-05-27 DE DE8787304684T patent/DE3779179D1/de not_active Expired - Fee Related
- 1987-05-27 EP EP87304684A patent/EP0247846B1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| US4746606A (en) | 1988-05-24 |
| EP0247846B1 (en) | 1992-05-20 |
| EP0247846A2 (en) | 1987-12-02 |
| JPH0358280B2 (enExample) | 1991-09-04 |
| DE3779179D1 (de) | 1992-06-25 |
| EP0247846A3 (en) | 1989-04-26 |
| JPS62285782A (ja) | 1987-12-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5418143A (en) | Test article and method for performing blood coagulation assays | |
| US5580744A (en) | Test article and method for performing blood coagulation assays | |
| ES2250472T3 (es) | Tiras de ensayo con membranas cargadas positivamente para analisis con tetrazolio. | |
| CA1236011A (en) | Assay method for whole blood samples | |
| EP0345781A2 (en) | Defined volume test device | |
| EP0071934B1 (en) | Method for preparing a color stable chromogenic analytical element | |
| EP1112376A1 (en) | Chromogen combinations for (per)oxidase-based determinations | |
| CA1112588A (en) | Bilirubin-specific fungal enzyme preparation | |
| JPH0721455B2 (ja) | 液体試料中の特定成分を分析するための用具および方法 | |
| US4746606A (en) | Bilirubin-specific enzyme and its analytical use | |
| JPH0695954B2 (ja) | クレアチニン又はクレアチンの測定要素及び測定方法 | |
| CA1226796A (en) | Reflective particle-containing analytical element | |
| EP0140004B1 (en) | Bilirubin-specific enzymes and their use in analytical elements and methods | |
| EP0169055B1 (en) | Polymeric single layer analytical element | |
| CA1056282A (en) | Multilayer analytical elements for use in the assay of cholesterol | |
| CA1278243C (en) | Analytical element containing photosensitive compound and filter layer and method of use | |
| EP0258035B1 (en) | Analytical element and method for theophylline determination having increased alkaline phosphatase isoenzyme | |
| JPS63305254A (ja) | クレアチニン又はクレアチンの分析用要素及び方法 | |
| AU742373B2 (en) | Competitive apo-peroxidase assay | |
| US4921804A (en) | Method for extracting bilirubin-specific enzyme preparation | |
| FUJIMURA et al. | A new method of blood galactose estimation for mass screening of galactosemia | |
| JPS6162865A (ja) | 抗原の検出方法 | |
| JPS60173471A (ja) | 抗原の検出方法 | |
| JPS6162864A (ja) | 抗原の検出方法 | |
| EP0341884A1 (en) | Novel reagent and method for assaying hydrogen peroxide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed |