CA1079189A - Immunoassay method utilizing magnetically attractable particles - Google Patents
Immunoassay method utilizing magnetically attractable particlesInfo
- Publication number
- CA1079189A CA1079189A CA273,631A CA273631A CA1079189A CA 1079189 A CA1079189 A CA 1079189A CA 273631 A CA273631 A CA 273631A CA 1079189 A CA1079189 A CA 1079189A
- Authority
- CA
- Canada
- Prior art keywords
- conduit
- particulate material
- reagent
- reaction mixture
- along
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000002245 particle Substances 0.000 title abstract description 18
- 238000003018 immunoassay Methods 0.000 title abstract description 12
- 239000011541 reaction mixture Substances 0.000 claims abstract description 92
- 238000000034 method Methods 0.000 claims abstract description 55
- 239000012530 fluid Substances 0.000 claims abstract description 45
- 239000007795 chemical reaction product Substances 0.000 claims abstract description 12
- 239000007790 solid phase Substances 0.000 claims description 95
- 239000000523 sample Substances 0.000 claims description 77
- 239000003153 chemical reaction reagent Substances 0.000 claims description 76
- 239000011236 particulate material Substances 0.000 claims description 70
- 239000007788 liquid Substances 0.000 claims description 60
- 239000000427 antigen Substances 0.000 claims description 41
- 239000007791 liquid phase Substances 0.000 claims description 39
- 102000036639 antigens Human genes 0.000 claims description 38
- 108091007433 antigens Proteins 0.000 claims description 38
- 230000005291 magnetic effect Effects 0.000 claims description 30
- 239000000470 constituent Substances 0.000 claims description 28
- 230000005484 gravity Effects 0.000 claims description 18
- 238000004458 analytical method Methods 0.000 claims description 16
- 239000012071 phase Substances 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 13
- 239000011159 matrix material Substances 0.000 claims description 11
- 238000009739 binding Methods 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 238000000926 separation method Methods 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 9
- 239000013618 particulate matter Substances 0.000 claims description 7
- 230000002285 radioactive effect Effects 0.000 claims description 7
- 239000000725 suspension Substances 0.000 claims description 6
- 239000007853 buffer solution Substances 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 241000700605 Viruses Species 0.000 claims description 3
- 239000000047 product Substances 0.000 claims description 3
- 238000011144 upstream manufacturing Methods 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 2
- 239000011368 organic material Substances 0.000 claims description 2
- 230000009257 reactivity Effects 0.000 claims description 2
- 108060003951 Immunoglobulin Proteins 0.000 claims 1
- 239000012805 animal sample Substances 0.000 claims 1
- -1 antibody Proteins 0.000 claims 1
- 102000018358 immunoglobulin Human genes 0.000 claims 1
- 238000004020 luminiscence type Methods 0.000 claims 1
- 238000005086 pumping Methods 0.000 claims 1
- 238000010584 magnetic trap Methods 0.000 abstract description 29
- 238000003556 assay Methods 0.000 abstract description 9
- 238000003127 radioimmunoassay Methods 0.000 abstract description 4
- 239000000872 buffer Substances 0.000 description 9
- 239000013060 biological fluid Substances 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- 239000006249 magnetic particle Substances 0.000 description 6
- 239000002699 waste material Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000011109 contamination Methods 0.000 description 3
- 238000005188 flotation Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 230000005294 ferromagnetic effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- 241000283986 Lepus Species 0.000 description 1
- 101100238304 Mus musculus Morc1 gene Proteins 0.000 description 1
- WGLPBDUCMAPZCE-UHFFFAOYSA-N Trioxochromium Chemical compound O=[Cr](=O)=O WGLPBDUCMAPZCE-UHFFFAOYSA-N 0.000 description 1
- 101150118507 WASL gene Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 229910000423 chromium oxide Inorganic materials 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 229910000428 cobalt oxide Inorganic materials 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Inorganic materials [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 1
- 235000013980 iron oxide Nutrition 0.000 description 1
- VBMVTYDPPZVILR-UHFFFAOYSA-N iron(2+);oxygen(2-) Chemical class [O-2].[Fe+2] VBMVTYDPPZVILR-UHFFFAOYSA-N 0.000 description 1
- 229910000480 nickel oxide Inorganic materials 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N2035/1025—Fluid level sensing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/804—Radioisotope, e.g. radioimmunoassay
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/808—Automated or kit
Landscapes
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA330,571A CA1080123A (en) | 1976-03-12 | 1979-06-26 | Particulate material for use in the analysis of liquid samples |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB10089/76A GB1575805A (en) | 1976-03-12 | 1976-03-12 | Automatic diagnostic apparatus |
Publications (1)
Publication Number | Publication Date |
---|---|
CA1079189A true CA1079189A (en) | 1980-06-10 |
Family
ID=9961275
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA273,631A Expired CA1079189A (en) | 1976-03-12 | 1977-03-10 | Immunoassay method utilizing magnetically attractable particles |
Country Status (16)
Families Citing this family (119)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2841782A1 (de) * | 1977-09-28 | 1979-04-12 | Technicon Instr | Immunanalysen sowie teilchenfoermiges reagenz zur verwendung bei immunanalysen |
NL7901025A (nl) * | 1978-02-13 | 1979-08-15 | Technicon Instr | Werkwijze voor het bereiden van magnetisch aantrekbaar materiaal en werkwijze voor de hiermee uit te voeren immunobepalingen. |
US4268268A (en) * | 1978-04-21 | 1981-05-19 | Blum Alvin S | Method and apparatus for characterization of cells, particles, and liquids |
US4264327A (en) * | 1978-04-21 | 1981-04-28 | Blum Alvin S | Method and apparatus for automatic competitive binding analysis |
US4277560A (en) * | 1978-10-24 | 1981-07-07 | Technicon Instruments Corporation | Enzyme immunoassays using immobilized reagents in a flowing stream |
US4230685A (en) * | 1979-02-28 | 1980-10-28 | Northwestern University | Method of magnetic separation of cells and the like, and microspheres for use therein |
US4297337A (en) * | 1979-04-13 | 1981-10-27 | Corning Glass Works | Solid-phase immunoassays using magnetic glass |
US4283201A (en) * | 1979-11-02 | 1981-08-11 | Phillips Petroleum Company | Method and apparatus suitable for repeated, accurate chemical analyses |
EP0030087A1 (en) * | 1979-11-13 | 1981-06-10 | Technicon Instruments Company Limited | Immunoassay method and apparatus and kit for carrying out the method |
EP0030086B2 (en) | 1979-11-13 | 1990-03-14 | TECHNICON INSTRUMENTS CORPORATION (a New York corporation) | Test-tube assembly, kit for making it and method of manual immunoassay |
US4481298A (en) * | 1981-04-13 | 1984-11-06 | Amf Incorporated | Pre-precipitated double antibody immunoassay method |
US4454234A (en) * | 1981-12-30 | 1984-06-12 | Czerlinski George H | Coated magnetizable microparticles, reversible suspensions thereof, and processes relating thereto |
JPS59195161A (ja) * | 1983-04-21 | 1984-11-06 | Fujirebio Inc | 磁性粒子及びその製造法 |
US4672040A (en) * | 1983-05-12 | 1987-06-09 | Advanced Magnetics, Inc. | Magnetic particles for use in separations |
US4628037A (en) * | 1983-05-12 | 1986-12-09 | Advanced Magnetics, Inc. | Binding assays employing magnetic particles |
US4698302A (en) * | 1983-05-12 | 1987-10-06 | Advanced Magnetics, Inc. | Enzymatic reactions using magnetic particles |
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BE637003A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1962-09-05 | |||
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1977
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- 1977-03-10 SE SE7702731A patent/SE7702731L/xx unknown
- 1977-03-10 BE BE175679A patent/BE852327A/xx not_active IP Right Cessation
- 1977-03-10 DE DE19772710438 patent/DE2710438A1/de not_active Withdrawn
- 1977-03-10 US US05/776,443 patent/US4141687A/en not_active Expired - Lifetime
- 1977-03-10 CA CA273,631A patent/CA1079189A/en not_active Expired
- 1977-03-11 DD DD7700197813A patent/DD129826A5/xx unknown
- 1977-03-11 NL NL7702647A patent/NL7702647A/xx not_active Application Discontinuation
- 1977-03-11 BR BR7701509A patent/BR7701509A/pt unknown
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- 1977-03-12 JP JP2753977A patent/JPS52141697A/ja active Granted
- 1977-03-14 IT IT67561/77A patent/IT1116284B/it active
- 1977-03-14 AR AR266841A patent/AR214993A1/es active
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1984
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Also Published As
Publication number | Publication date |
---|---|
GB1575805A (en) | 1980-10-01 |
AR214993A1 (es) | 1979-08-31 |
DD129826A5 (de) | 1978-02-08 |
DE2710438A1 (de) | 1977-11-03 |
FR2344019A1 (fr) | 1977-10-07 |
US4141687A (en) | 1979-02-27 |
BE852327A (fr) | 1977-09-12 |
IT1116284B (it) | 1986-02-10 |
AU2307277A (en) | 1978-09-14 |
JPS52141697A (en) | 1977-11-26 |
SE7702731L (sv) | 1977-09-13 |
AU508301B2 (en) | 1980-03-13 |
JPS60122374A (ja) | 1985-06-29 |
BR7701509A (pt) | 1978-01-03 |
JPS618388B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1986-03-13 |
FR2344019B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1982-04-02 |
NL7702647A (nl) | 1977-09-14 |
PL111132B1 (en) | 1980-08-30 |
JPS6250784B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1987-10-27 |
CH619537A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1980-09-30 |
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