BRPI0819929A2 - Expressão de amino ácido não natural em vivo na levedura pichia pastoris metilotrófica - Google Patents
Expressão de amino ácido não natural em vivo na levedura pichia pastoris metilotróficaInfo
- Publication number
- BRPI0819929A2 BRPI0819929A2 BRPI0819929-9A2A BRPI0819929A BRPI0819929A2 BR PI0819929 A2 BRPI0819929 A2 BR PI0819929A2 BR PI0819929 A BRPI0819929 A BR PI0819929A BR PI0819929 A2 BRPI0819929 A2 BR PI0819929A2
- Authority
- BR
- Brazil
- Prior art keywords
- live
- expression
- pichia pastoris
- acid amino
- methylotrophic yeast
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/84—Pichia
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US734107P | 2007-12-11 | 2007-12-11 | |
PCT/US2008/013568 WO2009075847A2 (en) | 2007-12-11 | 2008-12-10 | In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris |
Publications (1)
Publication Number | Publication Date |
---|---|
BRPI0819929A2 true BRPI0819929A2 (pt) | 2014-10-14 |
Family
ID=40756044
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0819929-9A2A BRPI0819929A2 (pt) | 2007-12-11 | 2008-12-10 | Expressão de amino ácido não natural em vivo na levedura pichia pastoris metilotrófica |
Country Status (11)
Country | Link |
---|---|
US (2) | US8647840B2 (pt) |
EP (2) | EP2231856A4 (pt) |
JP (3) | JP2011527886A (pt) |
KR (1) | KR20100105846A (pt) |
CN (2) | CN105647824A (pt) |
AU (1) | AU2008335778B2 (pt) |
BR (1) | BRPI0819929A2 (pt) |
CA (1) | CA2708760A1 (pt) |
IL (2) | IL206300A (pt) |
RU (1) | RU2010125104A (pt) |
WO (1) | WO2009075847A2 (pt) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2708760A1 (en) | 2007-12-11 | 2009-06-18 | The Scripps Research Institute | In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris |
WO2010053636A1 (en) * | 2008-11-06 | 2010-05-14 | Tate & Lyle Technology Ltd | Enhanced production and purification of a natural high intensity sweetener |
US8609383B2 (en) | 2008-12-10 | 2013-12-17 | The Scripps Research Institute | Production of carrier-peptide conjugates using chemically reactive unnatural amino acids |
DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
CN103145845A (zh) * | 2011-12-06 | 2013-06-12 | 吉林圣元科技有限责任公司 | Tat与人野生型P53融合蛋白在毕赤酵母中的表达 |
CN103060411B (zh) * | 2013-01-05 | 2014-02-05 | 义马煤业集团煤生化高科技工程有限公司 | 一种甲醇蛋白肽的生产方法 |
US10189892B2 (en) | 2013-03-15 | 2019-01-29 | Alderbio Holdings Llc | Fermentation process for antibody production |
CN104312932B (zh) * | 2014-10-24 | 2018-03-27 | 北京科兴中维生物技术有限公司 | 一种甲醇营养型酵母的蛋白诱导方法 |
US11008370B2 (en) * | 2015-10-26 | 2021-05-18 | Council Of Scientific & Industrial Research | Genetically modified yeast cell and improved process for production of clot-specific streptokinase |
EP3384021A1 (en) * | 2015-11-30 | 2018-10-10 | European Molecular Biology Laboratory | Means and methods for preparing engineered proteins by genetic code expansion in insect cells |
CN106770866A (zh) * | 2015-12-17 | 2017-05-31 | 中国医科大学 | 可实现人血清白蛋白鉴定及绝对定量的试剂盒及测定方法 |
CN105385693B (zh) * | 2015-12-25 | 2018-09-18 | 安阳工学院 | 一种高效表达多拷贝人表皮生长因子的方法 |
CN112142846B (zh) * | 2020-10-10 | 2022-07-08 | 西北大学 | Aox特异性抗体组合及其制备方法和用途 |
CN112940956A (zh) * | 2021-04-18 | 2021-06-11 | 江南大学 | 采用双启动子增强乳铁蛋白表达的毕赤酵母及其应用 |
Family Cites Families (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7229789B1 (en) | 2001-01-19 | 2007-06-12 | N.V. Organon | Methods and compositions for extracting proteins from cells |
JP2005502322A (ja) * | 2001-04-19 | 2005-01-27 | ザ スクリップス リサーチ インスティテュート | 非天然アミノ酸のインビボ組込み |
US6645739B2 (en) | 2001-07-26 | 2003-11-11 | Phoenix Pharmacologies, Inc. | Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same |
AU2003286436B2 (en) | 2002-10-16 | 2010-08-19 | The Scripps Research Institute | Glycoprotein synthesis |
CA2508939A1 (en) * | 2002-12-22 | 2004-07-15 | The Scripps Research Institute | Protein arrays |
EP1620491B1 (en) | 2003-04-03 | 2007-04-25 | Dow Corning Corporation | Moisture curable organosiloxane compositions |
CN101223272B (zh) * | 2003-04-17 | 2013-04-03 | 斯克利普斯研究院 | 扩展真核生物遗传密码 |
EP2793027B1 (en) * | 2003-06-18 | 2019-11-13 | The Scripps Research Institute | Unnatural reactive amino acid genetic code additions |
EP1704242A4 (en) | 2003-07-07 | 2008-06-18 | Scripps Research Inst | COMPOSITIONS WITH PAIRS OF ORTHOGONAL LEUCYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF |
US7527943B2 (en) * | 2003-07-07 | 2009-05-05 | The Scripps Research Institute | Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof |
PT1914314E (pt) | 2003-07-07 | 2010-09-08 | Scripps Research Inst | Composições de pares ortogonais de lisil-arnt e aminoacil-arnt-sintetase e suas utilizações |
EP1687401B1 (en) * | 2003-10-14 | 2012-11-21 | The Scripps Research Institute | Site-specific incorporation of redox active amino acids into proteins |
US7709604B2 (en) * | 2003-12-18 | 2010-05-04 | The Scripps Research Institute | Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells |
CA2567624A1 (en) * | 2004-05-25 | 2005-12-08 | The Scripps Research Institute | Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination |
EP1797177A4 (en) * | 2004-09-21 | 2008-07-09 | Scripps Research Inst | ADDITION OF PHOTOREGULATED AMINO ACIDS TO THE GENETIC CODE |
CN101151366B (zh) * | 2004-09-21 | 2012-12-19 | 斯克利普斯研究院 | 将炔基氨基酸体内掺入真细菌的蛋白 |
US20080171317A1 (en) * | 2004-09-22 | 2008-07-17 | The Scripps Research Institute | Site-Specific Labeling of Proteins for Nmr Studies |
JP2008516637A (ja) * | 2004-10-20 | 2008-05-22 | ザ スクリップス リサーチ インスティテュート | 真正細菌へのn−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み |
US7736872B2 (en) * | 2004-12-22 | 2010-06-15 | Ambrx, Inc. | Compositions of aminoacyl-TRNA synthetase and uses thereof |
SG158186A1 (en) * | 2004-12-22 | 2010-01-29 | Ambrx Inc | Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides |
EP2284191A3 (en) * | 2004-12-22 | 2011-07-20 | Ambrx, Inc. | Process for the preparation of hGH |
US7619128B2 (en) * | 2005-03-25 | 2009-11-17 | Exxonmobil Chemical Patents Inc. | Stabilizing catalyst activity during catalyst regeneration |
AU2006304110B2 (en) * | 2005-10-12 | 2012-02-02 | The Scripps Research Institute | Selective posttranslational modification of phage-displayed polypeptides |
EP2465870A1 (en) | 2005-11-07 | 2012-06-20 | Genentech, Inc. | Binding polypeptides with diversified and consensus VH/VL hypervariable sequences |
US8957187B2 (en) | 2005-12-02 | 2015-02-17 | Genentech, Inc. | Binding polypeptides and uses thereof |
AU2007223830A1 (en) * | 2006-03-09 | 2007-09-13 | The Scripps Research Institute | System for the expression of orthogonal translation components in eubacterial host cells |
US8217145B2 (en) * | 2006-03-16 | 2012-07-10 | The Scrips Research Institute | Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine |
JP5225266B2 (ja) | 2006-05-19 | 2013-07-03 | テバ ファーマシューティカル インダストリーズ リミティド | 融合タンパク質、その使用、およびその製造方法 |
EP2035554B1 (en) | 2006-06-29 | 2013-04-24 | The Board of Trustees of The Leland Stanford Junior University | Cell-free synthesis of proteins containing unnatural amino acids |
AU2008247815B2 (en) | 2007-05-02 | 2012-09-06 | Ambrx, Inc. | Modified interferon beta polypeptides and their uses |
CA2708760A1 (en) | 2007-12-11 | 2009-06-18 | The Scripps Research Institute | In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris |
US8609383B2 (en) * | 2008-12-10 | 2013-12-17 | The Scripps Research Institute | Production of carrier-peptide conjugates using chemically reactive unnatural amino acids |
-
2008
- 2008-12-10 CA CA2708760A patent/CA2708760A1/en not_active Abandoned
- 2008-12-10 RU RU2010125104/10A patent/RU2010125104A/ru not_active Application Discontinuation
- 2008-12-10 KR KR1020107015262A patent/KR20100105846A/ko not_active Application Discontinuation
- 2008-12-10 BR BRPI0819929-9A2A patent/BRPI0819929A2/pt not_active IP Right Cessation
- 2008-12-10 EP EP08860799A patent/EP2231856A4/en not_active Ceased
- 2008-12-10 AU AU2008335778A patent/AU2008335778B2/en not_active Ceased
- 2008-12-10 JP JP2010537955A patent/JP2011527886A/ja active Pending
- 2008-12-10 US US12/734,995 patent/US8647840B2/en active Active
- 2008-12-10 CN CN201610056340.5A patent/CN105647824A/zh active Pending
- 2008-12-10 EP EP14194373.8A patent/EP2889370A1/en not_active Withdrawn
- 2008-12-10 WO PCT/US2008/013568 patent/WO2009075847A2/en active Application Filing
- 2008-12-10 CN CN2008801266965A patent/CN101939410A/zh active Pending
-
2010
- 2010-06-10 IL IL206300A patent/IL206300A/en not_active IP Right Cessation
-
2013
- 2013-09-02 IL IL228238A patent/IL228238A/en not_active IP Right Cessation
- 2013-10-02 JP JP2013207554A patent/JP6073205B2/ja active Active
-
2014
- 2014-01-27 US US14/165,199 patent/US9732349B2/en active Active
-
2016
- 2016-04-15 JP JP2016082136A patent/JP2016144469A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
US20110014650A1 (en) | 2011-01-20 |
US9732349B2 (en) | 2017-08-15 |
RU2010125104A (ru) | 2012-01-20 |
JP6073205B2 (ja) | 2017-02-01 |
WO2009075847A3 (en) | 2009-09-11 |
JP2011527886A (ja) | 2011-11-10 |
JP2016144469A (ja) | 2016-08-12 |
US20140287464A1 (en) | 2014-09-25 |
CN105647824A (zh) | 2016-06-08 |
EP2889370A1 (en) | 2015-07-01 |
IL228238A (en) | 2014-09-30 |
EP2231856A2 (en) | 2010-09-29 |
AU2008335778B2 (en) | 2014-04-10 |
CN101939410A (zh) | 2011-01-05 |
US8647840B2 (en) | 2014-02-11 |
CA2708760A1 (en) | 2009-06-18 |
IL206300A0 (en) | 2010-12-30 |
EP2231856A4 (en) | 2011-05-18 |
KR20100105846A (ko) | 2010-09-30 |
JP2014039554A (ja) | 2014-03-06 |
AU2008335778A1 (en) | 2009-06-18 |
IL206300A (en) | 2014-07-31 |
WO2009075847A2 (en) | 2009-06-18 |
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Legal Events
Date | Code | Title | Description |
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B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE AS 5A E 6A ANUIDADES. |
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B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2329 DE 25-08-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |
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B350 | Update of information on the portal [chapter 15.35 patent gazette] |