BRPI0715036A2 - mÉtodo de expansço para cÉlulas-tronco adultas do sangue, particulamente sangue perifÉrico, e aplicaÇço relativa no campo mÉdico - Google Patents
mÉtodo de expansço para cÉlulas-tronco adultas do sangue, particulamente sangue perifÉrico, e aplicaÇço relativa no campo mÉdico Download PDFInfo
- Publication number
- BRPI0715036A2 BRPI0715036A2 BRPI0715036-9A BRPI0715036A BRPI0715036A2 BR PI0715036 A2 BRPI0715036 A2 BR PI0715036A2 BR PI0715036 A BRPI0715036 A BR PI0715036A BR PI0715036 A2 BRPI0715036 A2 BR PI0715036A2
- Authority
- BR
- Brazil
- Prior art keywords
- stem cells
- treatment
- mammals
- medication
- pharmaceutical composition
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 60
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 45
- 210000005259 peripheral blood Anatomy 0.000 title claims abstract description 21
- 239000011886 peripheral blood Substances 0.000 title claims abstract description 21
- 210000004369 blood Anatomy 0.000 title claims abstract description 20
- 239000008280 blood Substances 0.000 title claims abstract description 20
- 238000011282 treatment Methods 0.000 claims abstract description 48
- 238000000338 in vitro Methods 0.000 claims abstract description 17
- 238000000746 purification Methods 0.000 claims abstract description 14
- 230000010261 cell growth Effects 0.000 claims abstract description 3
- 102100028123 Macrophage colony-stimulating factor 1 Human genes 0.000 claims abstract 8
- 101710127797 Macrophage colony-stimulating factor 1 Proteins 0.000 claims abstract 8
- 210000004027 cell Anatomy 0.000 claims description 58
- 241000124008 Mammalia Species 0.000 claims description 22
- 239000003814 drug Substances 0.000 claims description 20
- 210000004504 adult stem cell Anatomy 0.000 claims description 18
- 229940079593 drug Drugs 0.000 claims description 18
- 230000003902 lesion Effects 0.000 claims description 18
- 239000008194 pharmaceutical composition Substances 0.000 claims description 16
- 241000282472 Canis lupus familiaris Species 0.000 claims description 13
- 241000283086 Equidae Species 0.000 claims description 12
- 208000027418 Wounds and injury Diseases 0.000 claims description 12
- 230000002860 competitive effect Effects 0.000 claims description 12
- 230000006378 damage Effects 0.000 claims description 11
- 210000002435 tendon Anatomy 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 9
- 230000000926 neurological effect Effects 0.000 claims description 9
- 206010017076 Fracture Diseases 0.000 claims description 8
- 239000004480 active ingredient Substances 0.000 claims description 8
- 208000014674 injury Diseases 0.000 claims description 8
- 210000003041 ligament Anatomy 0.000 claims description 8
- 230000003115 biocidal effect Effects 0.000 claims description 7
- 230000001684 chronic effect Effects 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 208000011580 syndromic disease Diseases 0.000 claims description 7
- 230000001154 acute effect Effects 0.000 claims description 6
- 239000002671 adjuvant Substances 0.000 claims description 6
- 230000004968 inflammatory condition Effects 0.000 claims description 6
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 6
- 208000007882 Gastritis Diseases 0.000 claims description 5
- 241000282414 Homo sapiens Species 0.000 claims description 5
- 208000012902 Nervous system disease Diseases 0.000 claims description 5
- 208000025966 Neurological disease Diseases 0.000 claims description 5
- 208000006735 Periostitis Diseases 0.000 claims description 5
- 208000010067 Pituitary ACTH Hypersecretion Diseases 0.000 claims description 5
- 208000020627 Pituitary-dependent Cushing syndrome Diseases 0.000 claims description 5
- 208000021945 Tendon injury Diseases 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 230000004054 inflammatory process Effects 0.000 claims description 5
- 210000004400 mucous membrane Anatomy 0.000 claims description 5
- 208000015122 neurodegenerative disease Diseases 0.000 claims description 5
- 201000008482 osteoarthritis Diseases 0.000 claims description 5
- 230000029058 respiratory gaseous exchange Effects 0.000 claims description 5
- 206010040882 skin lesion Diseases 0.000 claims description 5
- 231100000444 skin lesion Toxicity 0.000 claims description 5
- 208000001762 Gastric Dilatation Diseases 0.000 claims description 4
- 206010061218 Inflammation Diseases 0.000 claims description 4
- 206010033799 Paralysis Diseases 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 208000000509 infertility Diseases 0.000 claims description 4
- 230000036512 infertility Effects 0.000 claims description 4
- 231100000535 infertility Toxicity 0.000 claims description 4
- 230000003071 parasitic effect Effects 0.000 claims description 4
- 230000003612 virological effect Effects 0.000 claims description 4
- 229920001917 Ficoll Polymers 0.000 claims description 3
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 claims description 3
- 229930182566 Gentamicin Natural products 0.000 claims description 3
- 210000000988 bone and bone Anatomy 0.000 claims description 3
- 238000005194 fractionation Methods 0.000 claims description 3
- 229960002518 gentamicin Drugs 0.000 claims description 3
- 241000282326 Felis catus Species 0.000 claims description 2
- 239000003242 anti bacterial agent Substances 0.000 claims description 2
- 238000010253 intravenous injection Methods 0.000 claims description 2
- 102000007651 Macrophage Colony-Stimulating Factor Human genes 0.000 description 14
- 108010046938 Macrophage Colony-Stimulating Factor Proteins 0.000 description 14
- 241001465754 Metazoa Species 0.000 description 10
- 238000001990 intravenous administration Methods 0.000 description 10
- 230000007170 pathology Effects 0.000 description 10
- 238000001727 in vivo Methods 0.000 description 8
- 239000002953 phosphate buffered saline Substances 0.000 description 8
- 210000001778 pluripotent stem cell Anatomy 0.000 description 8
- 238000002604 ultrasonography Methods 0.000 description 8
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 7
- 238000001356 surgical procedure Methods 0.000 description 6
- 208000024891 symptom Diseases 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 208000030175 lameness Diseases 0.000 description 5
- 210000001616 monocyte Anatomy 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 206010052428 Wound Diseases 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 208000004998 Abdominal Pain Diseases 0.000 description 3
- 208000002881 Colic Diseases 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 206010061223 Ligament injury Diseases 0.000 description 3
- 238000002617 apheresis Methods 0.000 description 3
- 210000001185 bone marrow Anatomy 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 210000003414 extremity Anatomy 0.000 description 3
- 239000003102 growth factor Substances 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 238000002660 stem cell treatment Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000002560 therapeutic procedure Methods 0.000 description 3
- 238000002054 transplantation Methods 0.000 description 3
- FUFLCEKSBBHCMO-UHFFFAOYSA-N 11-dehydrocorticosterone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)C(=O)CO)C4C3CCC2=C1 FUFLCEKSBBHCMO-UHFFFAOYSA-N 0.000 description 2
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 2
- 241001112696 Clostridia Species 0.000 description 2
- MFYSYFVPBJMHGN-ZPOLXVRWSA-N Cortisone Chemical compound O=C1CC[C@]2(C)[C@H]3C(=O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 MFYSYFVPBJMHGN-ZPOLXVRWSA-N 0.000 description 2
- MFYSYFVPBJMHGN-UHFFFAOYSA-N Cortisone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)(O)C(=O)CO)C4C3CCC2=C1 MFYSYFVPBJMHGN-UHFFFAOYSA-N 0.000 description 2
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 2
- 101000800116 Homo sapiens Thy-1 membrane glycoprotein Proteins 0.000 description 2
- 206010043276 Teratoma Diseases 0.000 description 2
- 102100033523 Thy-1 membrane glycoprotein Human genes 0.000 description 2
- 210000000577 adipose tissue Anatomy 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 229940127219 anticoagulant drug Drugs 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 210000004439 collateral ligament Anatomy 0.000 description 2
- 229960004544 cortisone Drugs 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 description 2
- 238000002073 fluorescence micrograph Methods 0.000 description 2
- 238000002575 gastroscopy Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 230000009191 jumping Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000004379 membrane Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 210000001872 metatarsal bone Anatomy 0.000 description 2
- 230000001537 neural effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 231100000241 scar Toxicity 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 206010061728 Bone lesion Diseases 0.000 description 1
- 201000006474 Brain Ischemia Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010008120 Cerebral ischaemia Diseases 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 241000777300 Congiopodidae Species 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 241000283074 Equus asinus Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- 206010021639 Incontinence Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102000004218 Insulin-Like Growth Factor I Human genes 0.000 description 1
- 208000002260 Keloid Diseases 0.000 description 1
- 208000034693 Laceration Diseases 0.000 description 1
- 208000011644 Neurologic Gait disease Diseases 0.000 description 1
- 208000007117 Oral Ulcer Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 208000018737 Parkinson disease Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 206010034960 Photophobia Diseases 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 206010061363 Skeletal injury Diseases 0.000 description 1
- 208000031353 Systolic Murmurs Diseases 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 1
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 1
- 206010048049 Wrist fracture Diseases 0.000 description 1
- 229960004821 amikacin Drugs 0.000 description 1
- LKCWBDHBTVXHDL-RMDFUYIESA-N amikacin Chemical compound O([C@@H]1[C@@H](N)C[C@H]([C@@H]([C@H]1O)O[C@@H]1[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O1)O)NC(=O)[C@@H](O)CCN)[C@H]1O[C@H](CN)[C@@H](O)[C@H](O)[C@H]1O LKCWBDHBTVXHDL-RMDFUYIESA-N 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000002555 auscultation Methods 0.000 description 1
- 210000002459 blastocyst Anatomy 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 206010008118 cerebral infarction Diseases 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 208000023652 chronic gastritis Diseases 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 150000001887 cortisones Chemical class 0.000 description 1
- 238000002316 cosmetic surgery Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- 229960003638 dopamine Drugs 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 210000002683 foot Anatomy 0.000 description 1
- 208000020694 gallbladder disease Diseases 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 208000010726 hind limb paralysis Diseases 0.000 description 1
- 210000004124 hock Anatomy 0.000 description 1
- 210000003016 hypothalamus Anatomy 0.000 description 1
- 230000007124 immune defense Effects 0.000 description 1
- 229940127121 immunoconjugate Drugs 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 150000002540 isothiocyanates Chemical class 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 210000001117 keloid Anatomy 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 208000018883 loss of balance Diseases 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 230000000527 lymphocytic effect Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000002297 mitogenic effect Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000000450 navicular bone Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- VOFUROIFQGPCGE-UHFFFAOYSA-N nile red Chemical compound C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=CC(=O)C2=C1 VOFUROIFQGPCGE-UHFFFAOYSA-N 0.000 description 1
- 238000009806 oophorectomy Methods 0.000 description 1
- 210000004197 pelvis Anatomy 0.000 description 1
- 210000004976 peripheral blood cell Anatomy 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000008196 pharmacological composition Substances 0.000 description 1
- 238000011458 pharmacological treatment Methods 0.000 description 1
- 230000001817 pituitary effect Effects 0.000 description 1
- 230000000135 prohibitive effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 210000003131 sacroiliac joint Anatomy 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 210000003954 umbilical cord Anatomy 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 210000000689 upper leg Anatomy 0.000 description 1
- 210000003932 urinary bladder Anatomy 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/04—Drugs for skeletal disorders for non-specific disorders of the connective tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Physical Education & Sports Medicine (AREA)
- Rheumatology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Reproductive Health (AREA)
- Neurology (AREA)
- Cell Biology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- Hematology (AREA)
- Pulmonology (AREA)
- Neurosurgery (AREA)
- Pain & Pain Management (AREA)
- Pregnancy & Childbirth (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT000498A ITRM20060498A1 (it) | 2006-09-20 | 2006-09-20 | Metodo di espansione di cellule staminali adulte da sangue periferico e relativi usi in campo medico |
| ITRM2006A000498 | 2006-09-20 | ||
| PCT/EP2007/059531 WO2008034740A1 (en) | 2006-09-20 | 2007-09-11 | Expansion method for adult stem cells from blood, particularly peripheral blood, and relative application in medical field |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0715036A2 true BRPI0715036A2 (pt) | 2013-05-28 |
Family
ID=38126399
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0715036-9A BRPI0715036A2 (pt) | 2006-09-20 | 2007-09-11 | mÉtodo de expansço para cÉlulas-tronco adultas do sangue, particulamente sangue perifÉrico, e aplicaÇço relativa no campo mÉdico |
Country Status (20)
| Country | Link |
|---|---|
| US (2) | US8263400B2 (enExample) |
| EP (1) | EP2076588B1 (enExample) |
| JP (1) | JP5340941B2 (enExample) |
| KR (1) | KR101570446B1 (enExample) |
| CN (1) | CN101553565B (enExample) |
| AU (1) | AU2007299043B2 (enExample) |
| BR (1) | BRPI0715036A2 (enExample) |
| CA (1) | CA2663072C (enExample) |
| DK (1) | DK2076588T3 (enExample) |
| EA (1) | EA019305B1 (enExample) |
| ES (1) | ES2435080T3 (enExample) |
| HR (1) | HRP20131104T1 (enExample) |
| IL (1) | IL197575A (enExample) |
| IT (1) | ITRM20060498A1 (enExample) |
| MX (1) | MX2009003059A (enExample) |
| PL (1) | PL2076588T3 (enExample) |
| RS (1) | RS53039B (enExample) |
| SI (1) | SI2076588T1 (enExample) |
| WO (1) | WO2008034740A1 (enExample) |
| ZA (1) | ZA200901748B (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9132116B2 (en) | 2004-08-02 | 2015-09-15 | Willowcroft Pharm Inc. | Mast cell stabilizers to prevent or treat laminitis |
| ITUD20080058A1 (it) * | 2008-03-18 | 2009-09-19 | Thankstem S R L | Kit per la raccolta di sangue, preferibilmente periferico, per la produzione di cellule staminali |
| WO2010126544A1 (en) * | 2009-04-29 | 2010-11-04 | Willowcroft Pharm, Llc | Mast cell stabilizers to prevent or treat laminitis |
| KR101202836B1 (ko) * | 2010-08-27 | 2012-11-20 | 서울대학교산학협력단 | 세포응집을 이용한 인간 혈액 유래 혈구 세포괴의 유도와 이를 이용한 혈중 성체줄기세포 및 전구세포의 증폭방법 및 상기의 방법에 의해 제조된 줄기세포 |
| SG192254A1 (en) * | 2011-01-31 | 2013-08-30 | Royal Medical Group Pllc | Pluripotent stem cells and method of stimulating and extracting non-embryonic pluripotent stem cells from mammal blood and using reconstituted pluripotent stem cells to treat diseases including chronic obstructive pulmonary disease |
| BE1020480A5 (nl) | 2012-10-01 | 2013-11-05 | Global Stem Cell Technology | Werkwijze voor de isolatie van mesenchymale stamcellen uit bloed van zoogdieren, en gebruik ervan. |
| MX369097B (es) * | 2014-05-09 | 2019-10-29 | Thankstem S R L | Metodo para expandir las celulas madre adultas de sangre entera. |
| RU2644650C2 (ru) | 2014-12-01 | 2018-02-13 | Общество с ограниченной ответственностью "Т-Хелпер Клеточные Технологии" | Материал стволовых клеток и способ его получения |
| WO2016204230A1 (ja) * | 2015-06-17 | 2016-12-22 | 仁幸 小林 | 幹細胞投与方法、競走馬または競技馬の症状改善方法、注射用容器および幹細胞注射セット |
| RU2708329C2 (ru) | 2016-05-31 | 2019-12-05 | Общество с ограниченной ответственностью "Т-Хелпер Клеточные Технологии" | Материал стволовых клеток, композиции и способы применения |
| US11103537B2 (en) | 2016-06-23 | 2021-08-31 | Tithon Biotech Inc. | Cells expressing parathyroid hormone 1 receptor and uses thereof |
| RU2771328C2 (ru) * | 2016-06-23 | 2022-04-29 | Титон Биотек, Инк. | Клетки, экспрессирующие рецептор паратиреоидного гормона 1, и их применение |
| MX2021014118A (es) * | 2019-05-22 | 2021-12-10 | Thankstem S R L | Metodo para expandir celulas madre adultas a partir de sangre total. |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1436843A (zh) * | 2002-02-08 | 2003-08-20 | 刘颉 | 脐血造血干细胞的制作方法 |
| TWI288779B (en) * | 2002-03-28 | 2007-10-21 | Blasticon Biotech Forschung | Dedifferentiated, programmable stem cells of monocytic origin, and their production and use |
| CA2505394C (en) | 2002-11-07 | 2014-05-13 | University Of Chicago | Human stem cell materials and methods |
| CN1667119A (zh) * | 2004-03-11 | 2005-09-14 | 中国人民解放军第二军医大学 | 一种成体干细胞及其培养方法和用途 |
-
2006
- 2006-09-20 IT IT000498A patent/ITRM20060498A1/it unknown
-
2007
- 2007-09-11 HR HRP20131104TT patent/HRP20131104T1/hr unknown
- 2007-09-11 EP EP07820134.0A patent/EP2076588B1/en active Active
- 2007-09-11 RS RS20130499A patent/RS53039B/sr unknown
- 2007-09-11 JP JP2009528679A patent/JP5340941B2/ja not_active Expired - Fee Related
- 2007-09-11 WO PCT/EP2007/059531 patent/WO2008034740A1/en not_active Ceased
- 2007-09-11 CN CN200780034935.XA patent/CN101553565B/zh active Active
- 2007-09-11 AU AU2007299043A patent/AU2007299043B2/en not_active Ceased
- 2007-09-11 SI SI200731352T patent/SI2076588T1/sl unknown
- 2007-09-11 EA EA200970294A patent/EA019305B1/ru not_active IP Right Cessation
- 2007-09-11 BR BRPI0715036-9A patent/BRPI0715036A2/pt not_active Application Discontinuation
- 2007-09-11 MX MX2009003059A patent/MX2009003059A/es active IP Right Grant
- 2007-09-11 PL PL07820134T patent/PL2076588T3/pl unknown
- 2007-09-11 ES ES07820134T patent/ES2435080T3/es active Active
- 2007-09-11 KR KR1020097007818A patent/KR101570446B1/ko active Active
- 2007-09-11 CA CA2663072A patent/CA2663072C/en active Active
- 2007-09-11 DK DK07820134.0T patent/DK2076588T3/da active
-
2009
- 2009-03-11 ZA ZA200901748A patent/ZA200901748B/xx unknown
- 2009-03-12 IL IL197575A patent/IL197575A/en active IP Right Grant
- 2009-03-20 US US12/408,145 patent/US8263400B2/en active Active
-
2012
- 2012-08-16 US US13/587,417 patent/US20120308535A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ITRM20060498A1 (it) | 2008-03-21 |
| CA2663072C (en) | 2016-01-19 |
| PL2076588T3 (pl) | 2014-01-31 |
| EA200970294A1 (ru) | 2009-10-30 |
| RS53039B (sr) | 2014-04-30 |
| CA2663072A1 (en) | 2008-03-27 |
| WO2008034740A1 (en) | 2008-03-27 |
| DK2076588T3 (da) | 2013-11-11 |
| IL197575A0 (en) | 2011-08-01 |
| AU2007299043B2 (en) | 2013-06-27 |
| EP2076588B1 (en) | 2013-08-21 |
| HRP20131104T1 (hr) | 2013-12-20 |
| IL197575A (en) | 2013-06-27 |
| CN101553565A (zh) | 2009-10-07 |
| HK1138315A1 (en) | 2010-08-20 |
| JP2010504083A (ja) | 2010-02-12 |
| KR20090074044A (ko) | 2009-07-03 |
| US20100068189A1 (en) | 2010-03-18 |
| EP2076588A1 (en) | 2009-07-08 |
| CN101553565B (zh) | 2015-05-06 |
| AU2007299043A1 (en) | 2008-03-27 |
| EA019305B1 (ru) | 2014-02-28 |
| MX2009003059A (es) | 2009-04-01 |
| ZA200901748B (en) | 2010-03-31 |
| JP5340941B2 (ja) | 2013-11-13 |
| KR101570446B1 (ko) | 2015-11-27 |
| US20120308535A1 (en) | 2012-12-06 |
| US8263400B2 (en) | 2012-09-11 |
| ES2435080T3 (es) | 2013-12-18 |
| SI2076588T1 (sl) | 2013-12-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BRPI0715036A2 (pt) | mÉtodo de expansço para cÉlulas-tronco adultas do sangue, particulamente sangue perifÉrico, e aplicaÇço relativa no campo mÉdico | |
| JP2010504083A5 (enExample) | ||
| WO2014053420A1 (en) | Method for the isolation of mesenchymal stem cells from mammalian blood and use thereof | |
| ES2550456T3 (es) | Uso de una composición que contiene células madre mesenquimatosas derivadas de sangre de cordón umbilical humano para inducir diferenciación y proliferación de células precursoras neurales o células madre neurales a células neurales | |
| Beerts et al. | Desmitis of the accessory ligament of the equine deep digital flexor tendon: a regenerative approach | |
| ES2690253T3 (es) | Método para expandir células madre adultas a partir de sangre entera | |
| US8277795B2 (en) | Methods and compositions for treating motor neuron diseases comprising mesenchymal stem cells | |
| Peng et al. | Hyperbaric oxygen therapy combined with Schwann cell transplantation promotes spinal cord injury recovery | |
| US11020436B2 (en) | Multipotent and immunocompatible stem cell concentrate | |
| RU2286160C1 (ru) | Способ лечения травматических повреждений спинного мозга | |
| HK1138315B (en) | Expansion method for adult stem cells from blood, particularly peripheral blood, and relative application in medical field | |
| Bogers et al. | 4 Veterinary Medicine’s | |
| HAFEEZ et al. | THERAPEUTIC EFFECT OF BONE MARROW MESENCHYMAL STEM CELLS FOR TREATMENT OF INDUCED MILD TESTICULAR DEGENERATION IN DOGS | |
| JP2018111650A (ja) | 幹細胞投与方法、および競走馬または競技馬の症状改善方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B25A | Requested transfer of rights approved |
Owner name: THANKSTEM SRL (IT) |
|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] | ||
| B07E | Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette] | ||
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements |