BRPI0516556A - certas plantas com nìveis "não saturados"ou reduzidos de ácidos graxos nas sementes e óleo derivado das sementes - Google Patents

certas plantas com nìveis "não saturados"ou reduzidos de ácidos graxos nas sementes e óleo derivado das sementes

Info

Publication number
BRPI0516556A
BRPI0516556A BRPI0516556-3A BRPI0516556A BRPI0516556A BR PI0516556 A BRPI0516556 A BR PI0516556A BR PI0516556 A BRPI0516556 A BR PI0516556A BR PI0516556 A BRPI0516556 A BR PI0516556A
Authority
BR
Brazil
Prior art keywords
present
seeds
unsaturated
plants
delta
Prior art date
Application number
BRPI0516556-3A
Other languages
English (en)
Inventor
Mark Thompson
Sam Reddy
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36148921&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0516556(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of BRPI0516556A publication Critical patent/BRPI0516556A/pt
Publication of BRPI0516556B1 publication Critical patent/BRPI0516556B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19001—Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Edible Oils And Fats (AREA)
  • Fats And Perfumes (AREA)

Abstract

CERTAS PLANTAS COM NìVEIS "NãO SATURADOS" OU REDUZIDOS DE áCIDOS GRAXOS NAS SEMENTES E óLEO DERIVADO DAS SEMENTES. A presente invenção fornece óleo de canola "não saturado". A presente invenção fornece ainda sementes que podem ser utilizadas para produzir tais óleos. As plantas que produzem estas sementes também estão incluídas dentro da presente invenção. Tudo isso foi surpreendentemente conseguido através da utilização de um gene da delta-9 dessaturase na canola. Esta tecnologia pode ser aplicada a outras plantas como divulgado aqui. Os óleos da presente invenção possuem características particularmente vantajosas e perfis de ácidos graxos, que não tinham sido atingidos até agora. A presente invenção fornece ainda adicionalmente um gene da delta-9 dessaturase otimizado para plantas. A presente invenção fornece ainda adicionalmente um gene da delta-9 dessaturase otimizado para plantas. Em algumas modalidades preferidas, uma planta preferida compreende pelo menos duas cópias de um gene da delta-9 dessaturase da presente invenção. As sementes produzidas por tais plantas surpreendentemente não exibem efeitos de silenciamento gênico, mas ao invés disso possui ainda reduções surpreendentes nos níveis de saturados totais.
BRPI0516556A 2004-10-08 2005-10-07 composição alimentícia frita, processo de obtenção de uma composição alimentícia frita, processo de redução da gordura saturada na fração de óleo de sementes de uma planta transgênica de canola e polinucleotídeo BRPI0516556B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61753204P 2004-10-08 2004-10-08
PCT/US2005/036052 WO2006042049A2 (en) 2004-10-08 2005-10-07 Oil and seeds with reduced saturate levels of fatty acids

Publications (2)

Publication Number Publication Date
BRPI0516556A true BRPI0516556A (pt) 2008-09-09
BRPI0516556B1 BRPI0516556B1 (pt) 2019-12-31

Family

ID=36148921

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0516556A BRPI0516556B1 (pt) 2004-10-08 2005-10-07 composição alimentícia frita, processo de obtenção de uma composição alimentícia frita, processo de redução da gordura saturada na fração de óleo de sementes de uma planta transgênica de canola e polinucleotídeo

Country Status (14)

Country Link
US (1) US20180030463A1 (pt)
EP (3) EP2338328B1 (pt)
JP (2) JP5072597B2 (pt)
CN (2) CN102978153A (pt)
AR (2) AR051932A1 (pt)
AU (1) AU2005294298C1 (pt)
BR (1) BRPI0516556B1 (pt)
CA (1) CA2583380C (pt)
DK (1) DK1799027T3 (pt)
ES (2) ES2645787T3 (pt)
HU (1) HUE034217T2 (pt)
MX (2) MX2007004231A (pt)
PL (2) PL1799027T3 (pt)
WO (1) WO2006042049A2 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2545083T3 (es) * 2007-07-09 2015-09-08 Bayer Cropscience Nv Planta de Brassica que comprende alelos mutantes de acil graso-ACP tioesterasa
WO2011150028A2 (en) 2010-05-25 2011-12-01 Cargill, Incorporated Brassica plants yielding oils with a low alpha linolenic acid content
US9695434B2 (en) 2010-05-25 2017-07-04 Cargill, Incorporated Brassica plants yielding oils with a low alpha linolenic acid content
AR081302A1 (es) 2010-06-24 2012-08-01 Brookhaven Science Ass Llc Acumulacion de acidos grasos omega-7 ( -7) en semillas de plantas
DK2585600T3 (da) * 2010-06-24 2016-05-09 Dow Agrosciences Llc Reduktion af indholdet af mættet fedtsyre i plantefrø
JP2017537633A (ja) * 2014-12-19 2017-12-21 ダウ アグロサイエンシィズ エルエルシー 低飽和脂肪酸または無飽和脂肪酸トランスジェニックキャノーラの生成
US11237100B2 (en) * 2016-06-09 2022-02-01 Corteva Agriscience Llc Methods using FTIR for plant trait detection and trait introgression
CN108342371B (zh) * 2018-04-12 2022-06-24 南京农业大学 一种新型阿魏酸酯酶及其编码基因和应用
CN113151318B (zh) * 2021-03-17 2022-08-16 云南中烟工业有限责任公司 一种烟草淀粉分支酶基因NtGBE1及其应用

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4627192B1 (en) 1984-11-16 1995-10-17 Sigco Res Inc Sunflower products and methods for their production
US5638637A (en) 1987-12-31 1997-06-17 Pioneer Hi-Bred International, Inc. Production of improved rapeseed exhibiting an enhanced oleic acid content
ES2141125T3 (es) 1987-12-31 2000-03-16 Pioneer Hi Bred Int Colza con contenido en acido oleico mejorado.
US4948811A (en) 1988-01-26 1990-08-14 The Procter & Gamble Company Salad/cooking oil balanced for health benefits
US5057419A (en) 1988-09-22 1991-10-15 Rutgers University Genetically engineered plasmid and organisms for the production of specialized oils
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US6680426B2 (en) 1991-01-07 2004-01-20 Auburn University Genetic engineering of plant chloroplasts
US5932479A (en) 1988-09-26 1999-08-03 Auburn University Genetic engineering of plant chloroplasts
US5674485A (en) 1988-11-01 1997-10-07 The Regents Of The University Of California Insect diagnostic and control compositions with truncated JHE
US7037692B1 (en) 1990-03-16 2006-05-02 Calgene, Inc. Plant desaturases compositions and uses
DK0476093T3 (da) 1990-04-04 1997-06-02 Pioneer Hi Bred Int Fremstilling af forbedret rapsfrø, der udviser et reduceret indhold af mættet fedtsyre
US5861187A (en) 1990-08-30 1999-01-19 Cargill, Incorporated Oil from canola seed with altered fatty acid profiles and a method of producing oil
ATE195053T1 (de) 1990-08-30 2000-08-15 Cargill Inc Brassica-öle mit geänderten fettsäuregehalten
CA2106960C (en) 1991-04-09 2005-03-08 Jacqueline De Silva Plant promoter involved in controlling lipid biosynthesis in seeds
CA2340611C (en) 1991-09-30 2006-02-07 Cargill, Incorporated Defatted canola meal with reduced glucosilonate content
US6270828B1 (en) 1993-11-12 2001-08-07 Cargrill Incorporated Canola variety producing a seed with reduced glucosinolates and linolenic acid yielding an oil with low sulfur, improved sensory characteristics and increased oxidative stability
DK0616644T3 (da) 1991-12-04 2003-10-27 Du Pont Fedtsyredesaturase-gener fra planter
EP0628051B1 (en) 1992-02-12 2003-07-02 Chromagen, Inc. Applications of fluorescent n-nucleosides and fluorescent structural analogs of n-nucleosides
JPH0614667A (ja) * 1992-03-13 1994-01-25 Lubrizol Corp:The デサチュラーゼを使用しての植物油の改変
JP3527242B2 (ja) * 1993-04-27 2004-05-17 カージル，インコーポレーテッド 食用の非水素化カノラ油
US5912041A (en) * 1993-06-17 1999-06-15 Cargill, Incorporated Canola shortening for food applications
AU692791B2 (en) 1993-10-12 1998-06-18 Agrigenetics, Inc. Brassica napus variety AG019
BR9408435A (pt) 1993-12-28 1997-08-05 Kirin Brewery Gene para dessaturase de ácido graxo vetor contendo o dito gene planta transformada com o dito gene e processo para criar a dita planta
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5625130A (en) * 1995-03-07 1997-04-29 Pioneer Hi-Bred International, Inc. Oilseed Brassica bearing an endogenous oil wherein the levels of oleic, alpha-linolenic, and saturated fatty acids are simultaneously provided in an atypical highly beneficial distribution via genetic control
US6004782A (en) 1995-04-14 1999-12-21 Bioelastics Research Ltd. Hyperexpression of bioelastic polypeptides
DE69635905T2 (de) * 1995-12-14 2006-12-21 Cargill, Inc., Wayzata Pflanzen mit mutierten Sequenzen, welche ein veränderten Fettsäuregehalt vermitteln
US5850026A (en) 1996-07-03 1998-12-15 Cargill, Incorporated Canola oil having increased oleic acid and decreased linolenic acid content
US6100450A (en) 1997-10-22 2000-08-08 Rhone-Poulenc Agrochimie Seed specific promoters based on arabidopsis genes
US6586658B1 (en) 1998-03-06 2003-07-01 Metabolix, Inc. Modification of fatty acid metabolism in plants
DK1068342T3 (da) * 1998-03-30 2009-11-02 Dow Agrosciences Llc Modifikation af fedtsyresammensætning i planter ved ekspression af en Aspergillus nidulans delta-9 CoA desaturase
AU5785199A (en) 1998-08-24 2000-03-14 Rutgers, The State University Of New Jersey Synthetic fatty acid desaturase gene for expression in plants
NZ517467A (en) 1999-08-27 2004-10-29 Commw Scient Ind Res Org Flax seed specific promoters and methods for specifically expressing genes of interest in flax seeds
DE19950589A1 (de) 1999-10-20 2001-05-23 Gvs Ges Fuer Erwerb Und Verwer Elongasepromotoren für gewebespezifische Expression von Transgenen in Pflanzen
US6706950B2 (en) 2000-07-25 2004-03-16 Calgene Llc Nucleic acid sequences encoding β-ketoacyl-ACP synthase and uses thereof
WO2003014347A2 (en) 2000-12-01 2003-02-20 Michigan State University Plant seed specific promoters
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
US7294759B2 (en) 2001-06-29 2007-11-13 E. I. Du Pont De Nemours And Company Alteration of oil traits in plants
BR0309869A (pt) 2002-05-03 2005-04-26 Monsanto Technology Llc Promotores temporais de sementes para a expressão de genes em plantas

Also Published As

Publication number Publication date
CN101065013B (zh) 2012-11-07
JP2008515433A (ja) 2008-05-15
MX2007004231A (es) 2007-06-14
EP2338328B1 (en) 2017-09-20
WO2006042049A2 (en) 2006-04-20
PL1799027T3 (pl) 2012-10-31
ES2388066T3 (es) 2012-10-08
CN102978153A (zh) 2013-03-20
HUE034217T2 (en) 2018-01-29
DK1799027T3 (da) 2012-07-23
BRPI0516556B1 (pt) 2019-12-31
WO2006042049A3 (en) 2007-03-15
CN101065013A (zh) 2007-10-31
AR051932A1 (es) 2007-02-21
EP1799027A4 (en) 2008-08-27
EP1799027A2 (en) 2007-06-27
PL2338328T3 (pl) 2018-02-28
EP1799027B1 (en) 2012-06-20
CA2583380C (en) 2016-08-02
EP2338328A2 (en) 2011-06-29
AR108902A2 (es) 2018-10-10
MX348676B (es) 2017-06-23
AU2005294298B2 (en) 2012-02-16
JP2012210217A (ja) 2012-11-01
JP5072597B2 (ja) 2012-11-14
ES2645787T3 (es) 2017-12-07
AU2005294298A1 (en) 2006-04-20
US20180030463A1 (en) 2018-02-01
JP5753820B2 (ja) 2015-07-22
EP2338328A3 (en) 2011-07-27
CA2583380A1 (en) 2006-04-20
AU2005294298C1 (en) 2017-03-09
EP3318121A1 (en) 2018-05-09

Similar Documents

Publication Publication Date Title
BRPI0516556A (pt) certas plantas com nìveis "não saturados"ou reduzidos de ácidos graxos nas sementes e óleo derivado das sementes
BRPI0802241A2 (pt) células-tronco mesenquimais e seus usos
BRPI0600157A (pt) método para tratar uma planta, colheita, e, composição lìquida adequada para tratar plantas
BRPI0713357A2 (pt) Inibidores de proteína tirosina fosfatase humana e método de utilização.
BR112013002601A2 (pt) corticosteróides para o tratamento de dor em articulação
BRPI0512481A (pt) polinucleotìdeo isolado, polipeptìdeo, construção recombinante, célula, yarrowia sp.transformada, métodos para transformar uma célula, produzir uma planta transformada, produzir levedura, produzir ácidos graxos poliinsaturados e produzir pelo menos um ácido graxo poliinsaturados, sementes, óleos, plantas de sementes oleaginosa e alimentos ou rações
BRPI0604534A (pt) composição, e, método para tratamento de planta
BRPI0511355A (pt) composição para aplicação tópica na pele de mamìferos, seu uso, métodos para confecção de composições, método para o tratamento da psorìase e método para o tratamento do eczema
BR112015018113A2 (pt) composições compreendendo um agente de controle biológico baseado em streptomyces e um fungicida
BRPI0411479A (pt) métodos para a produção de insulina em plantas
HN2006042227A (es) Metodos y composiciones para ingenieria de cultivo y control de enfermedades
BR0106126A (pt) Processo para a preparação de ésteres de ácido graxo partindo de sementes ou de frutos
BR112014009097A2 (pt) composição para uso na promoção de crescimento de osso saudável e/ou na prevenção e/ou tratamento das doenças dos ossos
ES2180845T3 (es) Composiciones de silicona dispersables.
WO2008090474A3 (en) Production of rosmarinic acid from spearmint and uses thereof
BR112012001048A8 (pt) Composições adjuvantes agrícolas, composições de pesticidas, e métodos de utilização de tais composições.
CL2008001264A1 (es) Metodo para estimular el crecimiento de las plantas que comprende contactar el suelo o sustrato con una composicion biofertilizante que comprende una cepa de la bacteria tsukamurella parometabola.
BR112017011810A2 (pt) geração de canola transgênica com baixo ou sem ácidos graxos saturados
BR112016007902A2 (pt) rnas não codificantes regulatórios como determinantes de macho-esterilidade em gramas e outras plantas monocotiledôneas
BR0212246A (pt) Composição indutora da resistência a doença de planta, método para controlar uma doença de planta, e, processo para a produção de uma composição
BRPI0409139A (pt) combinação de ingredientes ativos de óleos que contêm ácido graxo w3 com extratos de plantas que contêm polifenol e uso destes
AR025222A1 (es) Agente con accion desodorante, producto, procedimiento para desodorizar, empleo del agente y procedimiento de elaboracion
BR0318602A (pt) óleo de girassol, planta de girassol, sementes de girassol, método para produção de uma planta que forma sementes contendo um óleo endógeno, planta hìbrida, progenitura de planta hìbrida, óleo, e produto alimentìcio
AR085212A1 (es) Metodos y composiciones para reducir la aglutinacion de semillas
BR112012018801A2 (pt) composição e processo para o controle de doenças de plantas

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06H Technical and formal requirements: requirement cancelled [chapter 6.8 patent gazette]

Free format text: ANULACAO DA EXIGENCIA 6.6 POR TER SIDO INDEVIDA REFERENTE A RPI NO 2199 DE 26/02/2013.

B15K Others concerning applications: alteration of classification

Ipc: C12N 15/82 (2006.01), A23D 9/00 (2006.01), A23L 33

B06T Formal requirements before examination [chapter 6.20 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 31/12/2019, OBSERVADAS AS CONDICOES LEGAIS.

B25D Requested change of name of applicant approved

Owner name: CORTEVA AGRISCIENCE LLC (US)

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 18A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2743 DE 01-08-2023 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.