BRPI0512798A - molécula de ácido nucleico recombinante, célula, método para produzir uma proteìna de interesse, uso de uma seqüência de ácido nucleico tendo atividade anti-repressora, métodos para gerar uma célula hospedeira expressando dois polipeptìdios de interesse, e para expressar dois polipeptìdios de interesse - Google Patents

molécula de ácido nucleico recombinante, célula, método para produzir uma proteìna de interesse, uso de uma seqüência de ácido nucleico tendo atividade anti-repressora, métodos para gerar uma célula hospedeira expressando dois polipeptìdios de interesse, e para expressar dois polipeptìdios de interesse

Info

Publication number
BRPI0512798A
BRPI0512798A BRPI0512798-0A BRPI0512798A BRPI0512798A BR PI0512798 A BRPI0512798 A BR PI0512798A BR PI0512798 A BRPI0512798 A BR PI0512798A BR PI0512798 A BRPI0512798 A BR PI0512798A
Authority
BR
Brazil
Prior art keywords
interest
nucleic acid
polypeptides
expressing
protein
Prior art date
Application number
BRPI0512798-0A
Other languages
English (en)
Inventor
Arie Pieter Otte
Theodorus Hendrikus Jaco Kwaks
Richard George Antonius Sewalt
Henricus Johannes Mar Blokland
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34971366&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0512798(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Publication of BRPI0512798A publication Critical patent/BRPI0512798A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

MOLéCULA DE áCIDO NUCLEICO RECOMBINANTE, CéLULA, MéTODO PARA PRODUZIR UMA PROTEìNA DE INTERESSE, USO DE UMA SEQüêNCIA DE áCIDO NUCLEICO TENDO ATIVIDADE ANTI-REPRESSORA, MéTODOS PARA GERAR UMA CéLULA HOSPEDEIRA EXPRESSANDO DOIS POLIPEPTìDIOS DE INTERESSE, E PARA EXPRESSAR DOIS POLIPEPTìDIOS DE INTERESSE. A invenção provê um novo elemento anti-repressor, que é útil para melhorar a expressão de ácido nucleico em células hospedeiras. Também são providos métodos usando o novo elemento anti-repressor para produzir uma proteína de interesse. A invenção também provê novas configurações de cassetes de expressão compreendendo elementos anti-repressores.
BRPI0512798-0A 2004-07-08 2005-07-07 molécula de ácido nucleico recombinante, célula, método para produzir uma proteìna de interesse, uso de uma seqüência de ácido nucleico tendo atividade anti-repressora, métodos para gerar uma célula hospedeira expressando dois polipeptìdios de interesse, e para expressar dois polipeptìdios de interesse BRPI0512798A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US58700704P 2004-07-08 2004-07-08
EP2004051405 2004-07-08
PCT/EP2005/053251 WO2006005718A2 (en) 2004-07-08 2005-07-07 Novel sequence for improving expression of nucleic acid

Publications (1)

Publication Number Publication Date
BRPI0512798A true BRPI0512798A (pt) 2008-04-08

Family

ID=34971366

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0512798-0A BRPI0512798A (pt) 2004-07-08 2005-07-07 molécula de ácido nucleico recombinante, célula, método para produzir uma proteìna de interesse, uso de uma seqüência de ácido nucleico tendo atividade anti-repressora, métodos para gerar uma célula hospedeira expressando dois polipeptìdios de interesse, e para expressar dois polipeptìdios de interesse

Country Status (20)

Country Link
US (1) US7825232B2 (pt)
JP (1) JP4805262B2 (pt)
KR (1) KR101188013B1 (pt)
CN (1) CN1981057B (pt)
AT (1) ATE378426T1 (pt)
AU (1) AU2005261699B2 (pt)
BR (1) BRPI0512798A (pt)
CA (1) CA2573022C (pt)
DE (1) DE602005003369T2 (pt)
DK (1) DK1763586T3 (pt)
EA (1) EA011747B1 (pt)
ES (1) ES2296201T3 (pt)
HK (1) HK1097004A1 (pt)
IL (1) IL180531A (pt)
MX (1) MXPA06014553A (pt)
NO (1) NO341862B1 (pt)
NZ (1) NZ551510A (pt)
PT (1) PT1763586E (pt)
WO (1) WO2006005718A2 (pt)
ZA (1) ZA200610470B (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60312039T2 (de) * 2002-12-20 2007-11-08 Chromagenics B.V. Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
ES2384391T3 (es) * 2004-11-08 2012-07-04 Chromagenics B.V. Selección de células hospedantes que expresan proteína a altos niveles
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
JP5291341B2 (ja) * 2004-11-08 2013-09-18 クロマジェニックス ベー ヴェー タンパク質を高レベルで発現する宿主細胞の選定
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
WO2010147462A2 (en) 2009-06-15 2010-12-23 Cellagenics B.V. Novel stringent selectable markers
WO2011159157A1 (en) 2010-06-15 2011-12-22 Cellagenics B.V. Novel intergenic elements for enhancing gene expression
US9650630B2 (en) 2010-09-01 2017-05-16 Cellagenics B.V. Nucleic acid fragments from a ribosomal protein promoter for enhancing gene expression
CN110484563B (zh) * 2019-07-25 2023-04-07 新乡医学院 哺乳动物细胞组合表达载体、表达系统、制备方法和应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60236331D1 (de) * 2001-07-04 2010-06-17 Chromagenics Bv DNS-Sequenzen mit Anti-Repressor-Aktivität
ES2362273T3 (es) * 2002-06-14 2011-06-30 Chromagenics B.V. Método para la producción simultánea de múltiples proteínas; vectores y células para su uso en el mismo.
DE60312039T2 (de) 2002-12-20 2007-11-08 Chromagenics B.V. Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können

Also Published As

Publication number Publication date
CA2573022A1 (en) 2006-01-19
IL180531A (en) 2011-04-28
IL180531A0 (en) 2007-06-03
US20070212755A1 (en) 2007-09-13
CN1981057A (zh) 2007-06-13
CN1981057B (zh) 2012-07-04
ZA200610470B (en) 2008-09-25
EA200700233A1 (ru) 2007-06-29
ES2296201T3 (es) 2008-04-16
KR101188013B1 (ko) 2012-10-08
DE602005003369T2 (de) 2009-03-05
EA011747B1 (ru) 2009-06-30
DK1763586T3 (da) 2008-03-17
KR20070042122A (ko) 2007-04-20
AU2005261699B2 (en) 2009-07-23
DE602005003369D1 (de) 2007-12-27
PT1763586E (pt) 2008-02-25
JP4805262B2 (ja) 2011-11-02
NO20070757L (no) 2007-02-08
NZ551510A (en) 2009-10-30
CA2573022C (en) 2012-03-06
JP2008505626A (ja) 2008-02-28
AU2005261699A1 (en) 2006-01-19
ATE378426T1 (de) 2007-11-15
WO2006005718A3 (en) 2006-08-17
MXPA06014553A (es) 2007-03-12
NO341862B1 (no) 2018-02-12
HK1097004A1 (en) 2007-06-15
US7825232B2 (en) 2010-11-02
WO2006005718A2 (en) 2006-01-19

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

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