BRPI0412471A - ensaio de alta eficácia à base de células mamìferas para a determinação do perfil e a seleção de moduladores putativos de um canal de sódio epitelial, métodos de monitorar a atividade de um canal de sódio epitelial, e de identificar um composto modulador de sabor salgado, um composto que modula henac, e um modulador de enac humano, e, oócito - Google Patents

ensaio de alta eficácia à base de células mamìferas para a determinação do perfil e a seleção de moduladores putativos de um canal de sódio epitelial, métodos de monitorar a atividade de um canal de sódio epitelial, e de identificar um composto modulador de sabor salgado, um composto que modula henac, e um modulador de enac humano, e, oócito

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Publication number
BRPI0412471A
BRPI0412471A BRPI0412471-5A BRPI0412471A BRPI0412471A BR PI0412471 A BRPI0412471 A BR PI0412471A BR PI0412471 A BRPI0412471 A BR PI0412471A BR PI0412471 A BRPI0412471 A BR PI0412471A
Authority
BR
Brazil
Prior art keywords
enac
sodium channel
henac
modulating compound
epithelial sodium
Prior art date
Application number
BRPI0412471-5A
Other languages
English (en)
Inventor
Guy Sevant
Hong Chang
Cyril Redcrow
Ray Sumita
Imran Clark
Original Assignee
Senomyx Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Senomyx Inc filed Critical Senomyx Inc
Publication of BRPI0412471A publication Critical patent/BRPI0412471A/pt

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Abstract

"ENSAIO DE ALTA EFICáCIA à BASE DE CéLULAS MAMìFERAS PARA A DETERMINAçãO DO PERFIL E A SELEçãO DE MODULADORES PUTATIVOS DE UM CANAL DE SóDIO EPITELIAL, MéTODOS DE MONITORAR A ATIVIDADE DE UM CANAL DE SóDIO EPITELIAL, E DE IDENTIFICAR UM COMPOSTO MODULADOR DE SABOR SALGADO, UM COMPOSTO QUE MODULA HENAC, E UM MODULADOR DE ENAC HUMANO, E, OóCITO". Em um aspecto, a presente invenção refere-se a um ensaio de alta eficácia baseado em células mamíferas para a formação de perfil e seleção de canal de sódio epitelial humano (hENaC, human epithelial sodium channel clonado de uma biblioteca de c-DNA de rim humano e também é expresso em outros tecidos, incluindo tecido do paladar humano. A presente invenção refere-se adicionalmente a ensaios eletrofisiológicos de média eficácia baseados em oócitos anfíbios para identificar moduladores de ENAC humano, de preferência, acentuadores de ENaC. Espera-se que compostos que modulam função de ENaC em um ensaio de ENaC à base de células venham a afetar o sabor salgado em humanos. Os ensaios aqui descritos apresentam vantagens importantes sobre sistemas de expressão celular existentes. No caso de células mamíferas, referidos ensaios podem ser realizados em placas de cultura convencionais de 96 ou 384 poços em modo de alta eficácia, sendo que se obtém resultados de ensaio acentuados com o uso de um composto que inibe função de ENaC, de preferência, um derivado de amilorida, como fenamila. No caso dos ensaios eletrofisiológicos de oócitos inventivos (técnica de pinça de voltagem com dois eletrodos), estes ensaios facilitam a identificação de compostos que modulam especificamente ENaC humano. Os ensaios da invenção proporcionam uma seleção robusta útil para detectar compostos que facilitam (acentuam) ou inibem função de hENaC. Compostos que acentuam ou bloqueiam atividade de canal ENaC humano deveriam, portanto, modular o sabor salgado em humanos.
BRPI0412471-5A 2003-07-10 2004-07-09 ensaio de alta eficácia à base de células mamìferas para a determinação do perfil e a seleção de moduladores putativos de um canal de sódio epitelial, métodos de monitorar a atividade de um canal de sódio epitelial, e de identificar um composto modulador de sabor salgado, um composto que modula henac, e um modulador de enac humano, e, oócito BRPI0412471A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48574503P 2003-07-10 2003-07-10
PCT/US2004/021853 WO2005014848A2 (en) 2003-07-10 2004-07-09 IMPROVED ELECTROPHYSIOLOGICAL ASSAYS USING OOCYTES THAT EXPRESS HUMAN ENaC AND THE USE OF PHENAMIL TO IMPROVE THE EFFECT OF ENaC ENHANCERS IN ASSAYS USING MEMBRANE POTENTIAL REPORTING DYES

Publications (1)

Publication Number Publication Date
BRPI0412471A true BRPI0412471A (pt) 2006-09-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0412471-5A BRPI0412471A (pt) 2003-07-10 2004-07-09 ensaio de alta eficácia à base de células mamìferas para a determinação do perfil e a seleção de moduladores putativos de um canal de sódio epitelial, métodos de monitorar a atividade de um canal de sódio epitelial, e de identificar um composto modulador de sabor salgado, um composto que modula henac, e um modulador de enac humano, e, oócito

Country Status (12)

Country Link
US (5) US8105792B2 (pt)
EP (1) EP1644738A2 (pt)
JP (1) JP2007528712A (pt)
KR (1) KR20060029680A (pt)
CN (1) CN1842709A (pt)
AU (1) AU2004263845A1 (pt)
BR (1) BRPI0412471A (pt)
CA (1) CA2530497A1 (pt)
IL (1) IL172933A0 (pt)
MX (1) MXPA06000266A (pt)
NO (1) NO20060078L (pt)
WO (1) WO2005014848A2 (pt)

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AU2007309516B2 (en) * 2006-10-19 2013-10-24 Monell Chemical Senses Center Human salty taste receptor and methods of modulating salty taste perception
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CN109364248B (zh) * 2018-10-16 2021-05-18 哈尔滨医科大学 ENaC及其抑制剂在预防、缓解和/或治疗动脉粥样硬化中的应用
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Also Published As

Publication number Publication date
US20120070857A1 (en) 2012-03-22
CN1842709A (zh) 2006-10-04
US9459259B2 (en) 2016-10-04
MXPA06000266A (es) 2006-03-30
US10048274B2 (en) 2018-08-14
US20190242905A1 (en) 2019-08-08
JP2007528712A (ja) 2007-10-18
US20160069899A9 (en) 2016-03-10
WO2005014848A2 (en) 2005-02-17
AU2004263845A1 (en) 2005-02-17
US8105792B2 (en) 2012-01-31
US20050059094A1 (en) 2005-03-17
US10215759B2 (en) 2019-02-26
IL172933A0 (en) 2006-06-11
NO20060078L (no) 2006-04-07
US20090123942A1 (en) 2009-05-14
KR20060029680A (ko) 2006-04-06
EP1644738A2 (en) 2006-04-12
CA2530497A1 (en) 2005-02-17
US20170175160A1 (en) 2017-06-22
WO2005014848A3 (en) 2005-05-06

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