NO20060078L - Forbedrede elektrofysiologiske tester som benytter oocytter som uttrykker human ENaC og anvendelse av fenamil for a forbedre effekten av ENaC-forsterkere i tester som benytter membran potensial rapporterende fargestoffer - Google Patents

Forbedrede elektrofysiologiske tester som benytter oocytter som uttrykker human ENaC og anvendelse av fenamil for a forbedre effekten av ENaC-forsterkere i tester som benytter membran potensial rapporterende fargestoffer

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Publication number
NO20060078L
NO20060078L NO20060078A NO20060078A NO20060078L NO 20060078 L NO20060078 L NO 20060078L NO 20060078 A NO20060078 A NO 20060078A NO 20060078 A NO20060078 A NO 20060078A NO 20060078 L NO20060078 L NO 20060078L
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Norway
Prior art keywords
enac
tests
human
enhance
compounds
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NO20060078A
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English (en)
Inventor
Guy Servant
Hong Chang
Cyril Redcrow
Ray Sumita
Imran Clark
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Senomyx Inc
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Publication date
Application filed by Senomyx Inc filed Critical Senomyx Inc
Publication of NO20060078L publication Critical patent/NO20060078L/no

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

I ett aspekt angår foreliggende oppfinnelse en mammaisk celle-basert høykapasitets test for profilering og screening av human epitelial natriumkanal (ENaC) klonet fra et humant nyre cDNA-bibliotek og uttrykkes også i andre vev inkludert humant smaksvev. Foreliggende oppfinnelse angår videre amfibie-oocyttbaserte medium-throughput elektrofysiologiske tester for å identifisere human ENaC-modulatorer, foretrukket ENaC-forsterkere. Forbindelser som modulerer ENaC-funksjon i en cellebasert ENaC-test forventes å påvirke saltsmak hos mennesker. Testene beskrevet heri har fordeler foran eksisterende cellulære ekspresjonssystemer. l tilfelle av mammaiske celler kan slike tester gjennomføres i standard 96- eller 384-brønners dyrkingsplater i høykapasitets metode hvor forsterkede testresultater oppnås ved anvendelse av en forbindelse som hemmer ENaC-funksjon, foretrukket et amilorid-derivat slik som fenamil. l tilfelle av de oocytt elektrofysiologiske testene ifølge foreliggende oppfinnelse (toelektroders spennings clamp-teknikk), letter disse testene identifikasjon av forbindelser som spesifikt modulerer human ENaC. Testene ifølge oppfinnelsen tilveiebringer et robust screen anvendelig for å detektere forbindelser som letter (forsterker) eller hemmer hENaC-funksjon. Forbindelse som forsterker eller blokkerer human EnaC kanal-aktivitet skulle derved modulere saltsmak hos mennesker.
NO20060078A 2003-07-10 2006-01-05 Forbedrede elektrofysiologiske tester som benytter oocytter som uttrykker human ENaC og anvendelse av fenamil for a forbedre effekten av ENaC-forsterkere i tester som benytter membran potensial rapporterende fargestoffer NO20060078L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48574503P 2003-07-10 2003-07-10
PCT/US2004/021853 WO2005014848A2 (en) 2003-07-10 2004-07-09 IMPROVED ELECTROPHYSIOLOGICAL ASSAYS USING OOCYTES THAT EXPRESS HUMAN ENaC AND THE USE OF PHENAMIL TO IMPROVE THE EFFECT OF ENaC ENHANCERS IN ASSAYS USING MEMBRANE POTENTIAL REPORTING DYES

Publications (1)

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NO20060078L true NO20060078L (no) 2006-04-07

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NO20060078A NO20060078L (no) 2003-07-10 2006-01-05 Forbedrede elektrofysiologiske tester som benytter oocytter som uttrykker human ENaC og anvendelse av fenamil for a forbedre effekten av ENaC-forsterkere i tester som benytter membran potensial rapporterende fargestoffer

Country Status (12)

Country Link
US (5) US9459259B2 (no)
EP (1) EP1644738A2 (no)
JP (1) JP2007528712A (no)
KR (1) KR20060029680A (no)
CN (1) CN1842709A (no)
AU (1) AU2004263845A1 (no)
BR (1) BRPI0412471A (no)
CA (1) CA2530497A1 (no)
IL (1) IL172933A0 (no)
MX (1) MXPA06000266A (no)
NO (1) NO20060078L (no)
WO (1) WO2005014848A2 (no)

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Also Published As

Publication number Publication date
WO2005014848A2 (en) 2005-02-17
EP1644738A2 (en) 2006-04-12
US9459259B2 (en) 2016-10-04
US8105792B2 (en) 2012-01-31
MXPA06000266A (es) 2006-03-30
WO2005014848A3 (en) 2005-05-06
US10215759B2 (en) 2019-02-26
AU2004263845A1 (en) 2005-02-17
US20120070857A1 (en) 2012-03-22
KR20060029680A (ko) 2006-04-06
US20190242905A1 (en) 2019-08-08
JP2007528712A (ja) 2007-10-18
BRPI0412471A (pt) 2006-09-19
CA2530497A1 (en) 2005-02-17
IL172933A0 (en) 2006-06-11
US20170175160A1 (en) 2017-06-22
US10048274B2 (en) 2018-08-14
US20090123942A1 (en) 2009-05-14
US20160069899A9 (en) 2016-03-10
US20050059094A1 (en) 2005-03-17
CN1842709A (zh) 2006-10-04

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