BRPI0409557A - proteìna inibidora recombinante de calicreìna, seqüência de dna, vetor de expressão, célula hospedeira, composição farmacêutica e seu uso, método de produção de proteìna inibidora recombinante de calicreìna e kit de diagnóstico para detecção de calicreìna - Google Patents

proteìna inibidora recombinante de calicreìna, seqüência de dna, vetor de expressão, célula hospedeira, composição farmacêutica e seu uso, método de produção de proteìna inibidora recombinante de calicreìna e kit de diagnóstico para detecção de calicreìna

Info

Publication number
BRPI0409557A
BRPI0409557A BRPI0409557-0A BRPI0409557A BRPI0409557A BR PI0409557 A BRPI0409557 A BR PI0409557A BR PI0409557 A BRPI0409557 A BR PI0409557A BR PI0409557 A BRPI0409557 A BR PI0409557A
Authority
BR
Brazil
Prior art keywords
recombinant
inhibitor protein
expression vector
dna sequence
kallikrein
Prior art date
Application number
BRPI0409557-0A
Other languages
English (en)
Inventor
David Deperthes
Sylvain Cloutier
Original Assignee
Univ Lausanne
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Lausanne filed Critical Univ Lausanne
Publication of BRPI0409557A publication Critical patent/BRPI0409557A/pt
Publication of BRPI0409557B1 publication Critical patent/BRPI0409557B1/pt
Publication of BRPI0409557B8 publication Critical patent/BRPI0409557B8/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00Drugs for disorders of the urinary system
    • A61P13/08Drugs for disorders of the urinary system of the prostate
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • A61P35/04Antineoplastic agents specific for metastasis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00Drugs for immunological or allergic disorders
    • A61P37/02Immunomodulators
    • A61P37/06Immunosuppressants, e.g. drugs for graft rejection
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/8107Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121Serpins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Oncology (AREA)
  • Rheumatology (AREA)
  • Urology & Nephrology (AREA)
  • Pain & Pain Management (AREA)
  • Transplantation (AREA)
  • Communicable Diseases (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"PROTEìNA INIBIDORA RECOMBINANTE DE CALICREìNA, SEQüêNCIA DE DNA, VETOR DE EXPRESSãO, CéLULA HOSPEDEIRA, COMPOSIçãO FARMACêUTICA E SEU USO, MéTODO DE PRODUçãO DE PROTEìNA INIBIDORA RECOMBINANTE DE CALICREìNA E KIT DE DIAGNóSTICO PARA DETECçãO DE CALICREìNA". A presente invenção refere-se a uma proteína inibidora quimérica de uma protease que compreende uma seqüência polipeptídica inibidora e pelo menos uma seqüência polipeptídica de um local de interação entre enzima e substrato específico para protease. Outros objetos da presente invenção são o fornecimento de uma seqüência de DNA isolada e purificada que codifica a proteína inibidora quimérica de uma protease, um vetor de expressão caracterizado pelo fato de que compreende a mencionada seqüência de DNA isolada e purificada, uma célula hospedeira eucariótica ou procariótica transformada com este vetor de expressão e um método de produção de uma proteína inibidora quimérica.
BRPI0409557-0 2003-04-04 2004-04-05 proteína recombinante inibidora de calicreína, sequência de dna, vetor de expressão, composição farmacêutica, uso da composição farmacêutica, método de produção da proteína recombinanete e kits de diagnóstico BRPI0409557B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US46034503P 2003-04-04 2003-04-04
US60/460,345 2003-04-04
PCT/IB2004/001040 WO2004087912A1 (en) 2003-04-04 2004-04-05 Inhibitor proteins of a protease and use thereof

Publications (3)

Publication Number Publication Date
BRPI0409557A true BRPI0409557A (pt) 2006-04-18
BRPI0409557B1 BRPI0409557B1 (pt) 2019-07-23
BRPI0409557B8 BRPI0409557B8 (pt) 2021-05-25

Family

ID=33131923

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0409557-0 BRPI0409557B8 (pt) 2003-04-04 2004-04-05 proteína recombinante inibidora de calicreína, sequência de dna, vetor de expressão, composição farmacêutica, uso da composição farmacêutica, método de produção da proteína recombinanete e kits de diagnóstico

Country Status (19)

Country Link
US (1) US8975370B2 (pt)
EP (1) EP1616007B1 (pt)
JP (1) JP4754478B2 (pt)
KR (1) KR101296367B1 (pt)
CN (1) CN1798838B (pt)
AU (1) AU2004225718B2 (pt)
BR (1) BRPI0409557B8 (pt)
CA (1) CA2521410C (pt)
CY (1) CY1114526T1 (pt)
DK (1) DK1616007T3 (pt)
ES (1) ES2431548T3 (pt)
IL (1) IL171158A (pt)
MX (1) MXPA05010574A (pt)
NO (1) NO334459B1 (pt)
PL (1) PL1616007T3 (pt)
PT (1) PT1616007E (pt)
SG (1) SG161106A1 (pt)
SI (1) SI1616007T1 (pt)
WO (1) WO2004087912A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2163652B2 (en) 2002-01-28 2023-05-31 Life Technologies Corporation Preparing crude biological extracts using protease, suitable for preparing cDNA
WO2006090282A2 (en) * 2005-02-28 2006-08-31 Universite De Lausanne Recombinant inhibitor proteins of an hk14 protease and use thereof
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
AU2009207394B2 (en) * 2008-01-21 2015-01-22 Dermadis Sa Use of serine protease inhibitors in the treatment of skin diseases
WO2010071833A1 (en) 2008-12-19 2010-06-24 Life Technologies Corporation Proteinase k inhibitors, methods and compositions therefor
JP5667094B2 (ja) * 2009-03-10 2015-02-12 エムイーディー ディスカバリー エスエー 好中球減少症の処置におけるセリンプロテアーゼ阻害剤の使用
SG195083A1 (en) * 2011-05-26 2013-12-30 Baxter Int Solid phase-bound elastase-binding assay for the measurement of alpha1,-antitrypsin activity
CN103044542B (zh) * 2012-08-17 2015-08-19 常熟理工学院 鲫鱼卵中丝氨酸蛋白酶抑制剂及其基因和应用
CN117169519B (zh) * 2023-10-26 2024-01-30 艾康生物技术(杭州)有限公司 用于检测样本中tt3和/或tt4的解离剂和试剂盒

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3773919A (en) 1969-10-23 1973-11-20 Du Pont Polylactide-drug mixtures
EP0462207B1 (en) 1989-03-06 2001-02-07 Board Of Regents, The University Of Texas System T-pa mutants resistant to inhibition by their cognate inhibitors
US5612194A (en) * 1989-06-23 1997-03-18 Trustees Of The University Of Pennsylvania Methods of producing effective recombinant serine protease inhibitors and uses of these inhibitors
US5827662A (en) * 1989-06-23 1998-10-27 The Trustees Of The University Of Pennsylvania Methods for detecting genetic mutations resulting in protease inhibitor insufficiencies
US5723316A (en) * 1989-06-23 1998-03-03 Trustees Of The University Of Pennsylvania α-1-antichymotrypsin analogues having chymase inhibiting activity
US6057287A (en) 1994-01-11 2000-05-02 Dyax Corp. Kallikrein-binding "Kunitz domain" proteins and analogues thereof
PT739355E (pt) 1994-01-11 2005-01-31 Dyax Corp Proteinas de 'dominio de kunitz' inibidoras de calicreina e seus analogos
US6284873B1 (en) 1999-03-12 2001-09-04 Hybritech Incorporated Complex of human kallikrein 2 (hK2) and protease inhibitor-6 (PI-6) in prostate tumor tissue and methods of using the complex

Also Published As

Publication number Publication date
JP2006523097A (ja) 2006-10-12
US8975370B2 (en) 2015-03-10
EP1616007B1 (en) 2013-07-24
AU2004225718A1 (en) 2004-10-14
PL1616007T3 (pl) 2013-12-31
CA2521410A1 (en) 2004-10-14
NO20055210D0 (no) 2005-11-04
WO2004087912A8 (en) 2005-01-20
WO2004087912A1 (en) 2004-10-14
BRPI0409557B8 (pt) 2021-05-25
JP4754478B2 (ja) 2011-08-24
KR20060010740A (ko) 2006-02-02
CY1114526T1 (el) 2016-10-05
CN1798838B (zh) 2012-01-04
SG161106A1 (en) 2010-05-27
BRPI0409557B1 (pt) 2019-07-23
MXPA05010574A (es) 2006-03-09
CA2521410C (en) 2014-06-03
DK1616007T3 (da) 2013-10-28
SI1616007T1 (sl) 2013-12-31
KR101296367B1 (ko) 2013-08-14
CN1798838A (zh) 2006-07-05
NO20055210L (no) 2005-12-23
IL171158A (en) 2011-05-31
PT1616007E (pt) 2013-10-28
ES2431548T3 (es) 2013-11-26
EP1616007A1 (en) 2006-01-18
AU2004225718B2 (en) 2009-11-05
NO334459B1 (no) 2014-03-10
US20060269536A1 (en) 2006-11-30

Similar Documents

Publication Publication Date Title
AR053063A2 (es) Proteina sustancialmente purificada y composicion farmaceutica que la comprende
IL217489A (en) U-pa polypeptides with specific substrate activity or activity and preparations and uses based on these polypeptides
ATE406439T1 (de) Eukaryontische signalsequenzen für polypeptid- expressions- und polypeptid- präsentationsbibliotheken
FI961886A (fi) Ihmisen Kunitz-tyyppiset proteaasi-inhibiittorit
NO20043891L (no) Anti-A-beta-antistoffer og deres anvendelse
DK1673457T3 (da) Nye svampeproteiner og nukleinsyrer, der koder for disse
ATE227739T1 (de) Hybridprotein zur hemmung der mastzelldegranulation und dessen verwendung
NO20055210D0 (no) Inhibitorproteiner av en protease og anvendelse derav
AR047729A1 (es) Anticuerpos terapeuticos que se unen a la apolipoproteina e (apoe)
BRPI0507613A (pt) expressão de plasminogênio e microplasminogênio em lentilha d'água
NO20073808L (no) BCMA polypeptider og anvendelser derav
BR112018003594A2 (pt) cadeia pesada de imunoglobulina recombinante, anticorpos, método de produção de conjugado e conjugados
ATE455792T1 (de) Neues phosphatbindendes protein, pharmazeutische zusammensetzungen, die dieses enthalten, und verwendung davon
PE20021098A1 (es) ANTICUERPOS ESPECIFICOS DE CD44v6
IL162568A0 (en) fluorescent protein from aequorea coerulscens and uses thereof
ATE412745T1 (de) Antiangiogene peptide vom n-terminus von endostatin
BRPI0413334A (pt) vetores de multiantìgenos para melanoma
Shibata et al. Improvement of biological activity and proteolytic stability of peptides by coupling with a cyclic peptide
Helmerhorst Whole saliva proteolysis: wealth of information for diagnostic exploitation
Ritchie et al. Characterization of crosslinking sites in fibrinogen for plasminogen activator inhibitor 2 (PAI‐2)
WO2006090282A3 (en) Recombinant inhibitor proteins of an hk14 protease and use thereof
ATE466876T1 (de) Immunogene konstrukte
WO2003010184A3 (en) A conserved xiap-interaction motif in caspase-9 and smac/diablo for mediating apoptosis
Kitano et al. Cotiarinase is a novel prothrombin activator from the venom of Bothrops cotiara
ATE213773T1 (de) In apoptosis beteiligte cysteine protease cmh-1 und dns-sequenzen dafür

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: AS CLASSIFICACOES ANTERIORES ERAM: C12N 15/09 , C12N 15/15 , C12N 9/64 , C12P 21/02

Ipc: C12N 15/15 (2006.01), C12N 9/64 (2006.01), C12P 21

B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notice of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06A Notification to applicant to reply to the report for non-patentability or inadequacy of the application [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 23/07/2019, OBSERVADAS AS CONDICOES LEGAIS. (CO) 10 (DEZ) ANOS CONTADOS A PARTIR DE 23/07/2019, OBSERVADAS AS CONDICOES LEGAIS

B16C Correction of notification of the grant

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 05/04/2004 OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF