BR9915734A - Método para a produção de hemoglobina livre de estroma - Google Patents
Método para a produção de hemoglobina livre de estromaInfo
- Publication number
- BR9915734A BR9915734A BR9915734-9A BR9915734A BR9915734A BR 9915734 A BR9915734 A BR 9915734A BR 9915734 A BR9915734 A BR 9915734A BR 9915734 A BR9915734 A BR 9915734A
- Authority
- BR
- Brazil
- Prior art keywords
- blood
- stroma
- red
- solution
- centrifuge
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 108010001708 stroma free hemoglobin Proteins 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 210000004369 blood Anatomy 0.000 abstract 6
- 239000008280 blood Substances 0.000 abstract 6
- 210000003743 erythrocyte Anatomy 0.000 abstract 2
- 108010054147 Hemoglobins Proteins 0.000 abstract 1
- 102000001554 Hemoglobins Human genes 0.000 abstract 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 1
- 210000000601 blood cell Anatomy 0.000 abstract 1
- 239000003633 blood substitute Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000000170 cell membrane Anatomy 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 239000012467 final product Substances 0.000 abstract 1
- 238000011065 in-situ storage Methods 0.000 abstract 1
- 230000036512 infertility Effects 0.000 abstract 1
- 239000006166 lysate Substances 0.000 abstract 1
- 229910052760 oxygen Inorganic materials 0.000 abstract 1
- 239000001301 oxygen Substances 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 230000035939 shock Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3692—Washing or rinsing blood or blood constituents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
- A61M1/3696—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
- A61M1/3698—Expressing processed fluid out from the turning rotor using another fluid compressing the treatment chamber; Variable volume rotors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/08—Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/04—Liquids
- A61M2202/0413—Blood
- A61M2202/0429—Red blood cells; Erythrocytes
- A61M2202/0433—Free haemoglobin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2205/00—General characteristics of the apparatus
- A61M2205/33—Controlling, regulating or measuring
- A61M2205/3306—Optical measuring means
- A61M2205/331—Optical measuring means used as turbidity change detectors, e.g. for priming-blood or plasma-hemoglubine-interface detection
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2205/00—General characteristics of the apparatus
- A61M2205/33—Controlling, regulating or measuring
- A61M2205/3317—Electromagnetic, inductive or dielectric measuring means
Landscapes
- Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- Anesthesiology (AREA)
- Cardiology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Diabetes (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- External Artificial Organs (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Centrifugal Separators (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
Patente de Invenção: <B>"MéTODO PARA A PRODUçãO DE HEMOGLOBINA LIVRE DE ESTROMA"<D>. O método emprega um separador de célula de sangue comercialmente disponível compreendendo uma centrífuga controlada por computador (2) tendo um rotor (24) no qual uma bolsa de processamento de sangue (6) contendo sangue de doador é colocado. Uma vez que o sangue é coletado, o processo é realizado totalmente dentro da tigela da centrífuga fechada, de preferência in situ no sítio de coleta do doador. Na primeira etapa, o sangue é centrifugado para separar o plasma dos componentes celulares. Depois do isolamento dos glóbulos vermelhos de outros componentes do sangue, as células vermelhas são lavadas com solução salina normal ou outra solução. Os glóbulos vermelhos são então lisados por choque hipotónico para separar as membranas de células vermelhas (estroma) e o lisado é coletado em um recipiente estéril(44), deixando apenas o estroma na tigela de centrífuga. O produto final pode ser usado como matéria-prima para qualquer um dos veículos de oxigênio à base de hemoglobulina atualmente sendo desenvolvido como substitutos de células vermelhas. Todas as etapas são realizadas dentro de um recipiente de processamento ou bolsa de sangue (6) na centrífuga de tigela para minimizar a manipulação e manter esterilidade. Um método para a preparação de uma solução hemoglobina modificada incorpora as etapas para a produção de hemoglobina livre de estroma, então adição de reagentes pré-medidos para reagir com a solução e filtração da solução.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10431998P | 1998-10-15 | 1998-10-15 | |
US12218099P | 1999-03-01 | 1999-03-01 | |
PCT/US1999/024149 WO2000021591A1 (en) | 1998-10-15 | 1999-10-15 | Method for production of stroma-free hemoglobin |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9915734A true BR9915734A (pt) | 2001-10-02 |
Family
ID=26801402
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9915734-9A BR9915734A (pt) | 1998-10-15 | 1999-10-15 | Método para a produção de hemoglobina livre de estroma |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP1121165A1 (pt) |
JP (1) | JP2002527149A (pt) |
KR (1) | KR20010099700A (pt) |
CN (1) | CN1332646A (pt) |
AU (1) | AU771465B2 (pt) |
BR (1) | BR9915734A (pt) |
CA (1) | CA2346825C (pt) |
MX (1) | MXPA01003836A (pt) |
WO (1) | WO2000021591A1 (pt) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005099858A1 (en) * | 2004-04-13 | 2005-10-27 | Sangart, Inc. | Methods and compositions for simultaneously isolating hemoglobin from red blood cells and inactivating viruses |
JP2006021121A (ja) | 2004-07-08 | 2006-01-26 | Hitachi Koki Co Ltd | 遠心分離機 |
ITLU20070013A1 (it) * | 2007-06-22 | 2008-12-23 | Kedrion Spa | Processo per la purificazione di emoglobina umana apirogena e virus inattivata. |
DK2520643T3 (da) * | 2007-12-07 | 2020-01-20 | Miltenyi Biotec Bv & Co Kg | Prøvebehandlingssystemer og -fremgangsmåder |
CA2791122C (en) * | 2010-02-25 | 2019-08-27 | Sangart, Inc. | Methods for preparing peg-hemoglobin conjugates using reduced reactant ratios |
US20150273132A1 (en) * | 2012-11-05 | 2015-10-01 | Haemonetics Corporation | System and Method for Continuous Separation of Whole Blood |
CN103091149B (zh) * | 2013-01-10 | 2015-08-05 | 济南金域医学检验中心有限公司 | 血红蛋白电泳液的制备方法 |
JP6067889B2 (ja) | 2013-02-26 | 2017-01-25 | イー・エム・デイー・ミリポア・コーポレイシヨン | 溶液条件を調整することによる活性化炭素を使用したタンパク質混合物からの1種のタンパク質の選択的除去 |
CN106456813B (zh) * | 2014-04-15 | 2020-06-30 | 勃林格殷格翰国际公司 | 在制造生物制品期间连续灭活病毒的方法、装置和系统 |
CN111801412A (zh) * | 2018-03-02 | 2020-10-20 | 赛默电子Led有限公司 | 用于分离生物悬浮液的一次性离心容器和使用方法 |
CN109298171B (zh) * | 2018-11-15 | 2022-06-28 | 武汉中生毓晋生物医药有限责任公司 | 醛化红细胞中内毒素的去除方法与应用 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4303193A (en) | 1979-01-22 | 1981-12-01 | Haemonetics Corporation | Apparatus for separating blood into components thereof |
US4401652A (en) * | 1980-12-31 | 1983-08-30 | Allied Corporation | Process for the preparation of stroma-free hemoglobin solutions |
US5464814A (en) * | 1986-06-20 | 1995-11-07 | Northfield Laboratories, Inc. | Acellular red blood cell substitute |
CA1312009C (en) | 1986-11-10 | 1992-12-29 | Carl W. Rausch | Extra pure semi-synthetic blood substitute |
JPH02121931A (ja) * | 1988-10-29 | 1990-05-09 | Res Inst For Prod Dev | 濃縮ヘモグロビンの製造方法 |
US5646252A (en) * | 1992-02-10 | 1997-07-08 | Staat Der Nederlanden, De Minister Van Defensie, Voor Deze: Het Hoofd Van De Afdeling Militair Geneeskundig Beleid | Hemoglobin composition and preparation thereof |
US5676644A (en) | 1995-06-07 | 1997-10-14 | Cobe Laboratories, Inc. | Extracorporeal blood processing methods and apparatus |
US5814601A (en) | 1997-02-28 | 1998-09-29 | The Regents Of The University Of California | Methods and compositions for optimization of oxygen transport by cell-free systems |
-
1999
- 1999-10-15 AU AU11170/00A patent/AU771465B2/en not_active Ceased
- 1999-10-15 CA CA002346825A patent/CA2346825C/en not_active Expired - Fee Related
- 1999-10-15 JP JP2000575563A patent/JP2002527149A/ja not_active Withdrawn
- 1999-10-15 WO PCT/US1999/024149 patent/WO2000021591A1/en not_active Application Discontinuation
- 1999-10-15 EP EP99954950A patent/EP1121165A1/en not_active Withdrawn
- 1999-10-15 BR BR9915734-9A patent/BR9915734A/pt not_active IP Right Cessation
- 1999-10-15 CN CN99813429A patent/CN1332646A/zh active Pending
- 1999-10-15 MX MXPA01003836A patent/MXPA01003836A/es unknown
- 1999-10-15 KR KR1020017004781A patent/KR20010099700A/ko not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
WO2000021591A1 (en) | 2000-04-20 |
CA2346825C (en) | 2008-07-29 |
EP1121165A1 (en) | 2001-08-08 |
CN1332646A (zh) | 2002-01-23 |
AU771465B2 (en) | 2004-03-25 |
KR20010099700A (ko) | 2001-11-09 |
AU1117000A (en) | 2000-05-01 |
MXPA01003836A (es) | 2002-09-18 |
CA2346825A1 (en) | 2000-04-20 |
JP2002527149A (ja) | 2002-08-27 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A E 7A ANUIDADES. |
|
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1865 DE 03/10/2006. |