CA2346825A1 - Method for production of stroma-free hemoglobin - Google Patents

Method for production of stroma-free hemoglobin Download PDF

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Publication number
CA2346825A1
CA2346825A1 CA002346825A CA2346825A CA2346825A1 CA 2346825 A1 CA2346825 A1 CA 2346825A1 CA 002346825 A CA002346825 A CA 002346825A CA 2346825 A CA2346825 A CA 2346825A CA 2346825 A1 CA2346825 A1 CA 2346825A1
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CA
Canada
Prior art keywords
blood
stroma
red
solution
centrifuge
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002346825A
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French (fr)
Other versions
CA2346825C (en
Inventor
Robert M. Winslow
Kim D. Vandegriff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sangart Inc
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2346825A1 publication Critical patent/CA2346825A1/en
Application granted granted Critical
Publication of CA2346825C publication Critical patent/CA2346825C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3692—Washing or rinsing blood or blood constituents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • A61M1/3696—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • A61M1/3698—Expressing processed fluid out from the turning rotor using another fluid compressing the treatment chamber; Variable volume rotors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/08—Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2202/00—Special media to be introduced, removed or treated
    • A61M2202/04—Liquids
    • A61M2202/0413—Blood
    • A61M2202/0429—Red blood cells; Erythrocytes
    • A61M2202/0433—Free haemoglobin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2205/00—General characteristics of the apparatus
    • A61M2205/33—Controlling, regulating or measuring
    • A61M2205/3306—Optical measuring means
    • A61M2205/331—Optical measuring means used as turbidity change detectors, e.g. for priming-blood or plasma-hemoglubine-interface detection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2205/00—General characteristics of the apparatus
    • A61M2205/33—Controlling, regulating or measuring
    • A61M2205/3317—Electromagnetic, inductive or dielectric measuring means

Landscapes

  • Health & Medical Sciences (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Anesthesiology (AREA)
  • Cardiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • External Artificial Organs (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Centrifugal Separators (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The method employs a commercially-available blood cell separator comprising a computer-controlled centrifuge (2) having a rotor (24) into which a blood processing bag (6) containing donor blood is placed. Once the blood is collected, the process is performed entirely within the enclosed centrifuge bowl, preferably in situ at the donor collection site. In the first step, the blood is centrifuged to separate the plasma from the cellular components.
After isolation of the red blood cells from other blood components, the red cells are washed with normal saline or other solution. The red blood cells are then lysed by hypotonic shock to separate the red cell membranes (stroma) and the lysate is collected in a sterile container (44), leaving only the stroma in the centrifuge bowl. The final product can be used as raw material for any of the hemoglobin-based oxygen carriers currently being developed as red cell substitutes. All of the steps are performed within a processing container or blood bag (6) in the bowl centrifuge to minimize handling and maintain sterility. A method for preparing a modified hemoglobin solution incorporates the steps for producing stroma-free hemoglobin, then adding pre-measured reagents to react with the solution and filtering the solution.
CA002346825A 1998-10-15 1999-10-15 Method for production of stroma-free hemoglobin Expired - Fee Related CA2346825C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US10431998P 1998-10-15 1998-10-15
US60/104,319 1998-10-15
US12218099P 1999-03-01 1999-03-01
US60/122,180 1999-03-01
PCT/US1999/024149 WO2000021591A1 (en) 1998-10-15 1999-10-15 Method for production of stroma-free hemoglobin

Publications (2)

Publication Number Publication Date
CA2346825A1 true CA2346825A1 (en) 2000-04-20
CA2346825C CA2346825C (en) 2008-07-29

Family

ID=26801402

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002346825A Expired - Fee Related CA2346825C (en) 1998-10-15 1999-10-15 Method for production of stroma-free hemoglobin

Country Status (9)

Country Link
EP (1) EP1121165A1 (en)
JP (1) JP2002527149A (en)
KR (1) KR20010099700A (en)
CN (1) CN1332646A (en)
AU (1) AU771465B2 (en)
BR (1) BR9915734A (en)
CA (1) CA2346825C (en)
MX (1) MXPA01003836A (en)
WO (1) WO2000021591A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080138790A1 (en) * 2004-04-13 2008-06-12 Winslow Robert M Methods and Compositions for Simultaneously Isolating Hemoglobin from Red Blood Cells and Inactivating Viruses
JP2006021121A (en) * 2004-07-08 2006-01-26 Hitachi Koki Co Ltd Centrifugal separator
ITLU20070013A1 (en) * 2007-06-22 2008-12-23 Kedrion Spa PROCESS FOR PURIFICATION OF HUMAN APROGENAL HEMOGLOBIN AND INACTIVATED VIRUS.
NO2227271T3 (en) * 2007-12-07 2018-06-23
CA2791122C (en) * 2010-02-25 2019-08-27 Sangart, Inc. Methods for preparing peg-hemoglobin conjugates using reduced reactant ratios
US20150273132A1 (en) * 2012-11-05 2015-10-01 Haemonetics Corporation System and Method for Continuous Separation of Whole Blood
CN103091149B (en) * 2013-01-10 2015-08-05 济南金域医学检验中心有限公司 The preparation method of HE liquid
JP6067889B2 (en) 2013-02-26 2017-01-25 イー・エム・デイー・ミリポア・コーポレイシヨン Selective removal of one protein from a protein mixture using activated carbon by adjusting solution conditions
WO2015158776A1 (en) * 2014-04-15 2015-10-22 Boehringer Ingelheim International Gmbh Methods, apparatuses, and systems for continuously inactivating a virus during manufacture of a biological product
EP3759208A1 (en) * 2018-03-02 2021-01-06 Thermo Electron LED GmbH Single-use centrifuge containers for separating biological suspensions and methods of use
CN109298171B (en) * 2018-11-15 2022-06-28 武汉中生毓晋生物医药有限责任公司 Method for removing endotoxin in aldehyde red blood cell and application thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4303193A (en) 1979-01-22 1981-12-01 Haemonetics Corporation Apparatus for separating blood into components thereof
US4401652A (en) * 1980-12-31 1983-08-30 Allied Corporation Process for the preparation of stroma-free hemoglobin solutions
US5464814A (en) * 1986-06-20 1995-11-07 Northfield Laboratories, Inc. Acellular red blood cell substitute
CA1312009C (en) 1986-11-10 1992-12-29 Carl W. Rausch Extra pure semi-synthetic blood substitute
JPH02121931A (en) * 1988-10-29 1990-05-09 Res Inst For Prod Dev Preparation of concentrated hemoglobin
US5646252A (en) * 1992-02-10 1997-07-08 Staat Der Nederlanden, De Minister Van Defensie, Voor Deze: Het Hoofd Van De Afdeling Militair Geneeskundig Beleid Hemoglobin composition and preparation thereof
US5676644A (en) 1995-06-07 1997-10-14 Cobe Laboratories, Inc. Extracorporeal blood processing methods and apparatus
US5814601A (en) 1997-02-28 1998-09-29 The Regents Of The University Of California Methods and compositions for optimization of oxygen transport by cell-free systems

Also Published As

Publication number Publication date
CN1332646A (en) 2002-01-23
WO2000021591A1 (en) 2000-04-20
CA2346825C (en) 2008-07-29
AU771465B2 (en) 2004-03-25
JP2002527149A (en) 2002-08-27
MXPA01003836A (en) 2002-09-18
AU1117000A (en) 2000-05-01
KR20010099700A (en) 2001-11-09
BR9915734A (en) 2001-10-02
EP1121165A1 (en) 2001-08-08

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